ID   NDUS8_MOUSE             Reviewed;         212 AA.
AC   Q8K3J1;
DT   10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 57.
DE   RecName: Full=NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial;
DE            EC=1.6.5.3;
DE            EC=1.6.99.3;
DE   AltName: Full=NADH-ubiquinone oxidoreductase 23 kDa subunit;
DE   AltName: Full=Complex I-23kD;
DE            Short=CI-23kD;
DE   Flags: Precursor;
GN   Name=Ndufs8;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Lucas-Teixeira V.A., Marques S., Belo J.A.;
RT   "Genomic organization of the mouse NDUFS8 gene encoding the NADH
RT   dehydrogenase:ubiquinone Fe-S protein 8.";
RL   Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 19-37; 60-90 AND 118-135, AND MASS SPECTROMETRY.
RC   STRAIN=C57BL/6; TISSUE=Brain;
RA   Lubec G., Kang S.U.;
RL   Submitted (APR-2007) to UniProtKB.
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory
CC       chain NADH dehydrogenase (Complex I) that is believed to belong to
CC       the minimal assembly required for catalysis. Complex I functions
CC       in the transfer of electrons from NADH to the respiratory chain.
CC       The immediate electron acceptor for the enzyme is believed to be
CC       ubiquinone (By similarity).
CC   -!- CATALYTIC ACTIVITY: NADH + ubiquinone = NAD(+) + ubiquinol.
CC   -!- CATALYTIC ACTIVITY: NADH + acceptor = NAD(+) + reduced acceptor.
CC   -!- COFACTOR: Binds 2 4Fe-4S clusters per subunit (By similarity).
CC   -!- SUBUNIT: Complex I is composed of 45 different subunits This is a
CC       component of the iron-sulfur (IP) fragment of the enzyme (By
CC       similarity).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion (By similarity).
CC   -!- SIMILARITY: Belongs to the complex I 23 kDa subunit family.
CC   -!- SIMILARITY: Contains 2 4Fe-4S ferredoxin-type domains.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AY096002; AAM34451.1; -; Genomic_DNA.
DR   IPI; IPI00170093; -.
DR   PIR; PC7079; PC7079.
DR   RefSeq; NP_659119.2; -.
DR   UniGene; Mm.44227; -.
DR   HSSP; P00198; 2FDN.
DR   PhosphoSite; Q8K3J1; -.
DR   REPRODUCTION-2DPAGE; Q8K3J1; -.
DR   PRIDE; Q8K3J1; -.
DR   Ensembl; ENSMUSG00000059734; Mus musculus.
DR   GeneID; 225887; -.
DR   KEGG; mmu:225887; -.
DR   NMPDR; fig|10090.3.peg.4372; -.
DR   MGI; MGI:2385079; Ndufs8.
DR   HOVERGEN; Q8K3J1; -.
DR   OMA; Q8K3J1; QADFLYR.
DR   BRENDA; 1.6.5.3; 244.
DR   BRENDA; 1.6.99.3; 244.
DR   NextBio; 377849; -.
DR   ArrayExpress; Q8K3J1; -.
DR   Bgee; Q8K3J1; -.
DR   CleanEx; MM_NDUFS8; -.
DR   GermOnline; ENSMUSG00000059734; Mus musculus.
DR   GO; GO:0005739; C:mitochondrion; IDA:MGI.
DR   GO; GO:0070469; C:respiratory chain; IEA:UniProtKB-KW.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:EC.
DR   GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
DR   GO; GO:0006810; P:transport; IEA:UniProtKB-KW.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR001450; 4Fe4S_Fe_S_bd_subgr.
DR   InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR   InterPro; IPR010226; NADH_quinone_OxRdtase_chainI.
DR   Pfam; PF00037; Fer4; 2.
DR   TIGRFAMs; TIGR01971; NuoI; 1.
DR   PROSITE; PS00198; 4FE4S_FER_1; 2.
DR   PROSITE; PS51379; 4FE4S_FER_2; 2.
PE   1: Evidence at protein level;
KW   4Fe-4S; Direct protein sequencing; Electron transport; Iron;
KW   Iron-sulfur; Metal-binding; Mitochondrion; NAD; Oxidoreductase;
KW   Repeat; Respiratory chain; Transit peptide; Transport; Ubiquinone.
FT   TRANSIT       1     34       Mitochondrion (By similarity).
FT   CHAIN        35    212       NADH dehydrogenase [ubiquinone] iron-
FT                                sulfur protein 8, mitochondrial.
FT                                /FTId=PRO_0000020014.
FT   DOMAIN      104    133       4Fe-4S ferredoxin-type 1.
FT   DOMAIN      143    172       4Fe-4S ferredoxin-type 2.
FT   METAL       113    113       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL       116    116       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL       119    119       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL       123    123       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       152    152       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       155    155       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       158    158       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       162    162       Iron-sulfur 1 (4Fe-4S) (By similarity).
SQ   SEQUENCE   212 AA;  24038 MW;  1E06E024EA7829CD CRC64;
     MYRLSSSMLP RALAQAMRTG HLNGQSLHSS AVAATYKYVN KKEQESEVDM KSATDNAARI
     LMWTELIRGL GMTLSYLFRE PATINYPFEK GPLSPRFRGE HALRRYPSGE ERCIACKLCE
     AICPAQAITI EAEPRADGSR RTTRYDIDMT KCIYCGFCQE ACPVDAIVEG PNFEFSTETH
     EELLYNKEKL LNNGDKWEAE IAANIQADYL YR
//