SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8K3J1

- NDUS8_MOUSE

UniProt

Q8K3J1 - NDUS8_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene
Ndufs8
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.UniRule annotation

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).UniRule annotation
NADH + acceptor = NAD+ + reduced acceptor.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi113 – 1131Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi116 – 1161Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi119 – 1191Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi123 – 1231Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi152 – 1521Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi155 – 1551Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi158 – 1581Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi162 – 1621Iron-sulfur 1 (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

GO - Biological processi

  1. mitochondrial respiratory chain complex I assembly Source: Ensembl
  2. response to oxidative stress Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-23kD
Short name:
CI-23kD
NADH-ubiquinone oxidoreductase 23 kDa subunit
Gene namesi
Name:Ndufs8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:2385079. Ndufs8.

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrial respiratory chain complex I Source: Ensembl
  2. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434Mitochondrion By similarityAdd
BLAST
Chaini35 – 212178NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialUniRule annotationPRO_0000020014Add
BLAST

Proteomic databases

MaxQBiQ8K3J1.
PaxDbiQ8K3J1.
PRIDEiQ8K3J1.

2D gel databases

REPRODUCTION-2DPAGEQ8K3J1.

PTM databases

PhosphoSiteiQ8K3J1.

Expressioni

Gene expression databases

ArrayExpressiQ8K3J1.
BgeeiQ8K3J1.
CleanExiMM_NDUFS8.
GenevestigatoriQ8K3J1.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.

Protein-protein interaction databases

BioGridi230440. 1 interaction.
IntActiQ8K3J1. 2 interactions.
MINTiMINT-1859976.

Structurei

3D structure databases

ProteinModelPortaliQ8K3J1.
SMRiQ8K3J1. Positions 66-191.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini104 – 133304Fe-4S ferredoxin-type 1Add
BLAST
Domaini143 – 172304Fe-4S ferredoxin-type 2Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiCOG1143.
GeneTreeiENSGT00390000003049.
HOGENOMiHOG000228289.
HOVERGENiHBG006547.
InParanoidiQ8K3J1.
KOiK03941.
OMAiTYKYVNL.
OrthoDBiEOG7J9VQT.
PhylomeDBiQ8K3J1.
TreeFamiTF105610.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K3J1-1 [UniParc]FASTAAdd to Basket

« Hide

MYRLSSSMLP RALAQAMRTG HLNGQSLHSS AVAATYKYVN KKEQESEVDM    50
KSATDNAARI LMWTELIRGL GMTLSYLFRE PATINYPFEK GPLSPRFRGE 100
HALRRYPSGE ERCIACKLCE AICPAQAITI EAEPRADGSR RTTRYDIDMT 150
KCIYCGFCQE ACPVDAIVEG PNFEFSTETH EELLYNKEKL LNNGDKWEAE 200
IAANIQADYL YR 212
Length:212
Mass (Da):24,038
Last modified:October 1, 2002 - v1
Checksum:i1E06E024EA7829CD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY096002 Genomic DNA. Translation: AAM34451.1.
CCDSiCCDS29402.1.
PIRiPC7079.
RefSeqiNP_001258372.1. NM_001271443.1.
NP_001258373.1. NM_001271444.1.
NP_659119.2. NM_144870.5.
UniGeneiMm.44227.

Genome annotation databases

EnsembliENSMUST00000075092; ENSMUSP00000074600; ENSMUSG00000059734.
GeneIDi225887.
KEGGimmu:225887.
UCSCiuc008fxn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY096002 Genomic DNA. Translation: AAM34451.1 .
CCDSi CCDS29402.1.
PIRi PC7079.
RefSeqi NP_001258372.1. NM_001271443.1.
NP_001258373.1. NM_001271444.1.
NP_659119.2. NM_144870.5.
UniGenei Mm.44227.

3D structure databases

ProteinModelPortali Q8K3J1.
SMRi Q8K3J1. Positions 66-191.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 230440. 1 interaction.
IntActi Q8K3J1. 2 interactions.
MINTi MINT-1859976.

PTM databases

PhosphoSitei Q8K3J1.

2D gel databases

REPRODUCTION-2DPAGE Q8K3J1.

Proteomic databases

MaxQBi Q8K3J1.
PaxDbi Q8K3J1.
PRIDEi Q8K3J1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000075092 ; ENSMUSP00000074600 ; ENSMUSG00000059734 .
GeneIDi 225887.
KEGGi mmu:225887.
UCSCi uc008fxn.1. mouse.

Organism-specific databases

CTDi 4728.
MGIi MGI:2385079. Ndufs8.

Phylogenomic databases

eggNOGi COG1143.
GeneTreei ENSGT00390000003049.
HOGENOMi HOG000228289.
HOVERGENi HBG006547.
InParanoidi Q8K3J1.
KOi K03941.
OMAi TYKYVNL.
OrthoDBi EOG7J9VQT.
PhylomeDBi Q8K3J1.
TreeFami TF105610.

Miscellaneous databases

NextBioi 377849.
PROi Q8K3J1.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8K3J1.
Bgeei Q8K3J1.
CleanExi MM_NDUFS8.
Genevestigatori Q8K3J1.

Family and domain databases

HAMAPi MF_01351. NDH1_NuoI.
InterProi IPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view ]
Pfami PF12838. Fer4_7. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01971. NuoI. 1 hit.
PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic organization of the mouse NDUFS8 gene encoding the NADH dehydrogenase:ubiquinone Fe-S protein 8."
    Lucas-Teixeira V.A., Marques S., Belo J.A.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 19-37; 60-90 AND 118-135, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiNDUS8_MOUSE
AccessioniPrimary (citable) accession number: Q8K3J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi