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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene

Ndufs8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi113Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi116Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi119Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi123Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi152Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi155Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi158Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi162Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiR-MMU-611105. Respiratory electron transport.
R-MMU-6799198. Complex I biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-23kD
Short name:
CI-23kD
NADH-ubiquinone oxidoreductase 23 kDa subunit
Gene namesi
Name:Ndufs8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2385079. Ndufs8.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial respiratory chain complex I Source: MGI
  • mitochondrion Source: MGI

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 34MitochondrionBy similarityAdd BLAST34
ChainiPRO_000002001435 – 212NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialAdd BLAST178

Proteomic databases

EPDiQ8K3J1.
MaxQBiQ8K3J1.
PaxDbiQ8K3J1.
PRIDEiQ8K3J1.

2D gel databases

REPRODUCTION-2DPAGEiQ8K3J1.

PTM databases

iPTMnetiQ8K3J1.
PhosphoSitePlusiQ8K3J1.
SwissPalmiQ8K3J1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000059734.
CleanExiMM_NDUFS8.
ExpressionAtlasiQ8K3J1. baseline and differential.
GenevisibleiQ8K3J1. MM.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.By similarity

Protein-protein interaction databases

BioGridi230440. 1 interactor.
IntActiQ8K3J1. 2 interactors.
MINTiMINT-1859976.
STRINGi10090.ENSMUSP00000074600.

Structurei

3D structure databases

ProteinModelPortaliQ8K3J1.
SMRiQ8K3J1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 1334Fe-4S ferredoxin-type 1Add BLAST30
Domaini143 – 1724Fe-4S ferredoxin-type 2Add BLAST30

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG3256. Eukaryota.
COG1143. LUCA.
GeneTreeiENSGT00390000003049.
HOGENOMiHOG000228289.
HOVERGENiHBG006547.
InParanoidiQ8K3J1.
KOiK03941.
OMAiLCGLCIE.
OrthoDBiEOG091G0OQD.
PhylomeDBiQ8K3J1.
TreeFamiTF105610.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI. 1 hit.
InterProiView protein in InterPro
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
PfamiView protein in Pfam
PF12838. Fer4_7. 1 hit.
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiView protein in PROSITE
PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K3J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRLSSSMLP RALAQAMRTG HLNGQSLHSS AVAATYKYVN KKEQESEVDM
60 70 80 90 100
KSATDNAARI LMWTELIRGL GMTLSYLFRE PATINYPFEK GPLSPRFRGE
110 120 130 140 150
HALRRYPSGE ERCIACKLCE AICPAQAITI EAEPRADGSR RTTRYDIDMT
160 170 180 190 200
KCIYCGFCQE ACPVDAIVEG PNFEFSTETH EELLYNKEKL LNNGDKWEAE
210
IAANIQADYL YR
Length:212
Mass (Da):24,038
Last modified:October 1, 2002 - v1
Checksum:i1E06E024EA7829CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY096002 Genomic DNA. Translation: AAM34451.1.
CCDSiCCDS29402.1.
PIRiPC7079.
RefSeqiNP_001258372.1. NM_001271443.1.
NP_001258373.1. NM_001271444.1.
NP_659119.2. NM_144870.5.
UniGeneiMm.44227.

Genome annotation databases

EnsembliENSMUST00000075092; ENSMUSP00000074600; ENSMUSG00000059734.
GeneIDi225887.
KEGGimmu:225887.
UCSCiuc008fxn.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNDUS8_MOUSE
AccessioniPrimary (citable) accession number: Q8K3J1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: October 1, 2002
Last modified: June 7, 2017
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families