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Reviewed, UniProtKB/Swiss-Prot Q8K3J1 (NDUS8_MOUSE)

Last modified November 3, 2009. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    NADH-ubiquinone oxidoreductase 23 kDa subunit
    Complex I-23kD
      Short name=CI-23kD
Gene names
Name: Ndufs8
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length212 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.

Catalytic activity

NADH + ubiquinone = NAD+ + ubiquinol.

NADH + acceptor = NAD+ + reduced acceptor.

Cofactor

Binds 2 4Fe-4S clusters per subunit By similarity.

Subunit structure

Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.

Subcellular location

Mitochondrion By similarity.

Sequence similarities

Belongs to the complex I 23 kDa subunit family.

Contains 2 4Fe-4S ferredoxin-type domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3434Mitochondrion By similarity
Chain35 – 212178NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
PRO_0000020014

Regions

Domain104 – 133304Fe-4S ferredoxin-type 1
Domain143 – 172304Fe-4S ferredoxin-type 2

Sites

Metal binding1131Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1161Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1191Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1231Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1521Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1551Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1581Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1621Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K3J1-1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 1E06E024EA7829CD

FASTA21224,038
        10         20         30         40         50         60 
MYRLSSSMLP RALAQAMRTG HLNGQSLHSS AVAATYKYVN KKEQESEVDM KSATDNAARI 

        70         80         90        100        110        120 
LMWTELIRGL GMTLSYLFRE PATINYPFEK GPLSPRFRGE HALRRYPSGE ERCIACKLCE 

       130        140        150        160        170        180 
AICPAQAITI EAEPRADGSR RTTRYDIDMT KCIYCGFCQE ACPVDAIVEG PNFEFSTETH 

       190        200        210 
EELLYNKEKL LNNGDKWEAE IAANIQADYL YR 

« Hide

References

[1]"Genomic organization of the mouse NDUFS8 gene encoding the NADH dehydrogenase:ubiquinone Fe-S protein 8."
Lucas-Teixeira V.A., Marques S., Belo J.A.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 19-37; 60-90 AND 118-135, MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

AY096002 Genomic DNA. Translation: AAM34451.1.
IPIIPI00170093.
PIRPC7079.
RefSeqNP_659119.2.
UniGeneMm.44227

3D structure databases

HSSPHSSP built from PDB template 2FDN based on UniProtKB P00198.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ8K3J1.

PTM databases

PhosphoSiteQ8K3J1.

2-D gel databases

REPRODUCTION-2DPAGEQ8K3J1.

Proteomic databases

PRIDEQ8K3J1.

Genome annotation databases

EnsemblENSMUST00000075092; ENSMUSP00000074600; ENSMUSG00000059734; Mus musculus. [Genome view]
GeneID225887.
KEGGmmu:225887.
NMPDRfig|10090.3.peg.4372.
UCSCuc008fxn.1. mouse.

Organism-specific databases

CTD225887.
MGIMGI:2385079. Ndufs8.

Phylogenomic databases

HOVERGENQ8K3J1.
OMAQADFLYR.

Enzyme and pathway databases

BRENDA1.6.5.3. 244.
1.6.99.3. 244.

Gene expression databases

ArrayExpressQ8K3J1.
BgeeQ8K3J1.
CleanExMM_NDUFS8.
GenevestigatorQ8K3J1.
GermOnlineENSMUSG00000059734. Mus musculus.

Family and domain databases

InterProIPR017896. 4Fe4S_Fe-S-bd.
IPR001450. 4Fe4S_Fe_S_bd_subgr.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamPF00037. Fer4. 2 hits.
[Graphical view]
TIGRFAMsTIGR01971. NuoI. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio377849.
SOURCESearch...

Entry information

Entry nameNDUS8_MOUSE
AccessionPrimary (citable) accession number: Q8K3J1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: October 1, 2002
Last modified: November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents