NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial precursor

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Q8K3J1 (NDUS8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 89. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
Complex I-23kD
Short name=CI-23kD
NADH-ubiquinone oxidoreductase 23 kDa subunit

Gene names

Name:

Ndufs8

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD⁺ + ubiquinol. NADH + acceptor = NAD⁺ + reduced acceptor.
Cofactor	Binds 2 4Fe-4S clusters per subunit By similarity.
Subunit structure	Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the complex I 23 kDa subunit family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Domain	Repeat Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial respiratory chain complex I assembly Inferred from electronic annotation. Source: Compara response to oxidative stress Inferred from electronic annotation. Source: Compara
Cellular_component	mitochondrial respiratory chain complex I Inferred from electronic annotation. Source: Compara mitochondrion Inferred from direct assay PubMed 14651853 PubMed 18614015. Source: MGI
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 34

Mitochondrion By similarity

Chain

35 – 212

178

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

PRO_0000020014

Regions

Domain

104 – 133

4Fe-4S ferredoxin-type 1

Domain

143 – 172

4Fe-4S ferredoxin-type 2

Sites

Metal binding

113

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

116

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

119

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

123

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

152

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

155

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

158

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

162

Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q8K3J1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 1E06E024EA7829CD
Show »

FASTA

212

24,038

        10         20         30         40         50         60 
MYRLSSSMLP RALAQAMRTG HLNGQSLHSS AVAATYKYVN KKEQESEVDM KSATDNAARI 

        70         80         90        100        110        120 
LMWTELIRGL GMTLSYLFRE PATINYPFEK GPLSPRFRGE HALRRYPSGE ERCIACKLCE 

       130        140        150        160        170        180 
AICPAQAITI EAEPRADGSR RTTRYDIDMT KCIYCGFCQE ACPVDAIVEG PNFEFSTETH 

       190        200        210 
EELLYNKEKL LNNGDKWEAE IAANIQADYL YR

« Hide

References

[1]	"Genomic organization of the mouse NDUFS8 gene encoding the NADH dehydrogenase:ubiquinone Fe-S protein 8." Lucas-Teixeira V.A., Marques S., Belo J.A. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 19-37; 60-90 AND 118-135, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY096002 Genomic DNA. Translation: AAM34451.1.
IPI	IPI00170093.
PIR	PC7079.
RefSeq	NP_001258372.1. NM_001271443.1. NP_001258373.1. NM_001271444.1. NP_659119.2. NM_144870.5.
UniGene	Mm.44227.
3D structure databases
ProteinModelPortal	Q8K3J1.
SMR	Q8K3J1. Positions 86-191.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8K3J1. 1 interaction.
PTM databases
PhosphoSite	Q8K3J1.
2D gel databases
REPRODUCTION-2DPAGE	Q8K3J1.
Proteomic databases
PaxDb	Q8K3J1.
PRIDE	Q8K3J1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000075092; ENSMUSP00000074600; ENSMUSG00000059734.
GeneID	225887.
KEGG	mmu:225887.
Organism-specific databases
CTD	4728.
MGI	MGI:2385079. Ndufs8.
Phylogenomic databases
eggNOG	COG1143.
GeneTree	ENSGT00390000003049.
HOGENOM	HOG000228289.
HOVERGEN	HBG006547.
InParanoid	Q8K3J1.
KO	K03941.
OMA	CPAMAIT.
OrthoDB	EOG43R3NR.
Gene expression databases
ArrayExpress	Q8K3J1.
Bgee	Q8K3J1.
CleanEx	MM_NDUFS8.
Genevestigator	Q8K3J1.
GermOnline	ENSMUSG00000059734. Mus musculus.
Family and domain databases
Gene3D	1.10.1060.10. 1 hit.
InterPro	IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR012285. Fum_reductase_C. IPR010226. NADH_quinone_OxRdtase_chainI. [Graphical view]
Pfam	PF12838. Fer4_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR01971. NuoI. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	377849.
SOURCE	Search...

Entry information

Entry name

NDUS8_MOUSE

Accession

Primary (citable) accession number: Q8K3J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents