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Q8K3J1

- NDUS8_MOUSE

UniProt

Q8K3J1 - NDUS8_MOUSE

Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene

Ndufs8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.By similarity

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi113 – 1131Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi116 – 1161Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi119 – 1191Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi123 – 1231Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi152 – 1521Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi155 – 1551Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi158 – 1581Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi162 – 1621Iron-sulfur 1 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

    GO - Biological processi

    1. mitochondrial respiratory chain complex I assembly Source: Ensembl
    2. response to oxidative stress Source: Ensembl

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Complex I-23kD
    Short name:
    CI-23kD
    NADH-ubiquinone oxidoreductase 23 kDa subunit
    Gene namesi
    Name:Ndufs8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:2385079. Ndufs8.

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrial respiratory chain complex I Source: Ensembl
    2. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3434MitochondrionBy similarityAdd
    BLAST
    Chaini35 – 212178NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialPRO_0000020014Add
    BLAST

    Proteomic databases

    MaxQBiQ8K3J1.
    PaxDbiQ8K3J1.
    PRIDEiQ8K3J1.

    2D gel databases

    REPRODUCTION-2DPAGEQ8K3J1.

    PTM databases

    PhosphoSiteiQ8K3J1.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8K3J1.
    BgeeiQ8K3J1.
    CleanExiMM_NDUFS8.
    GenevestigatoriQ8K3J1.

    Interactioni

    Subunit structurei

    Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.By similarity

    Protein-protein interaction databases

    BioGridi230440. 1 interaction.
    IntActiQ8K3J1. 2 interactions.
    MINTiMINT-1859976.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K3J1.
    SMRiQ8K3J1. Positions 66-191.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini104 – 133304Fe-4S ferredoxin-type 1Add
    BLAST
    Domaini143 – 172304Fe-4S ferredoxin-type 2Add
    BLAST

    Sequence similaritiesi

    Belongs to the complex I 23 kDa subunit family.Curated
    Contains 2 4Fe-4S ferredoxin-type domains.Curated

    Keywords - Domaini

    Repeat, Transit peptide

    Phylogenomic databases

    eggNOGiCOG1143.
    GeneTreeiENSGT00390000003049.
    HOGENOMiHOG000228289.
    HOVERGENiHBG006547.
    InParanoidiQ8K3J1.
    KOiK03941.
    OMAiTYKYVNL.
    OrthoDBiEOG7J9VQT.
    PhylomeDBiQ8K3J1.
    TreeFamiTF105610.

    Family and domain databases

    HAMAPiMF_01351. NDH1_NuoI.
    InterProiIPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR010226. NADH_quinone_OxRdtase_chainI.
    [Graphical view]
    PfamiPF12838. Fer4_7. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01971. NuoI. 1 hit.
    PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8K3J1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYRLSSSMLP RALAQAMRTG HLNGQSLHSS AVAATYKYVN KKEQESEVDM    50
    KSATDNAARI LMWTELIRGL GMTLSYLFRE PATINYPFEK GPLSPRFRGE 100
    HALRRYPSGE ERCIACKLCE AICPAQAITI EAEPRADGSR RTTRYDIDMT 150
    KCIYCGFCQE ACPVDAIVEG PNFEFSTETH EELLYNKEKL LNNGDKWEAE 200
    IAANIQADYL YR 212
    Length:212
    Mass (Da):24,038
    Last modified:October 1, 2002 - v1
    Checksum:i1E06E024EA7829CD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY096002 Genomic DNA. Translation: AAM34451.1.
    CCDSiCCDS29402.1.
    PIRiPC7079.
    RefSeqiNP_001258372.1. NM_001271443.1.
    NP_001258373.1. NM_001271444.1.
    NP_659119.2. NM_144870.5.
    UniGeneiMm.44227.

    Genome annotation databases

    EnsembliENSMUST00000075092; ENSMUSP00000074600; ENSMUSG00000059734.
    GeneIDi225887.
    KEGGimmu:225887.
    UCSCiuc008fxn.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY096002 Genomic DNA. Translation: AAM34451.1 .
    CCDSi CCDS29402.1.
    PIRi PC7079.
    RefSeqi NP_001258372.1. NM_001271443.1.
    NP_001258373.1. NM_001271444.1.
    NP_659119.2. NM_144870.5.
    UniGenei Mm.44227.

    3D structure databases

    ProteinModelPortali Q8K3J1.
    SMRi Q8K3J1. Positions 66-191.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 230440. 1 interaction.
    IntActi Q8K3J1. 2 interactions.
    MINTi MINT-1859976.

    PTM databases

    PhosphoSitei Q8K3J1.

    2D gel databases

    REPRODUCTION-2DPAGE Q8K3J1.

    Proteomic databases

    MaxQBi Q8K3J1.
    PaxDbi Q8K3J1.
    PRIDEi Q8K3J1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000075092 ; ENSMUSP00000074600 ; ENSMUSG00000059734 .
    GeneIDi 225887.
    KEGGi mmu:225887.
    UCSCi uc008fxn.1. mouse.

    Organism-specific databases

    CTDi 4728.
    MGIi MGI:2385079. Ndufs8.

    Phylogenomic databases

    eggNOGi COG1143.
    GeneTreei ENSGT00390000003049.
    HOGENOMi HOG000228289.
    HOVERGENi HBG006547.
    InParanoidi Q8K3J1.
    KOi K03941.
    OMAi TYKYVNL.
    OrthoDBi EOG7J9VQT.
    PhylomeDBi Q8K3J1.
    TreeFami TF105610.

    Miscellaneous databases

    NextBioi 377849.
    PROi Q8K3J1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8K3J1.
    Bgeei Q8K3J1.
    CleanExi MM_NDUFS8.
    Genevestigatori Q8K3J1.

    Family and domain databases

    HAMAPi MF_01351. NDH1_NuoI.
    InterProi IPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR010226. NADH_quinone_OxRdtase_chainI.
    [Graphical view ]
    Pfami PF12838. Fer4_7. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01971. NuoI. 1 hit.
    PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic organization of the mouse NDUFS8 gene encoding the NADH dehydrogenase:ubiquinone Fe-S protein 8."
      Lucas-Teixeira V.A., Marques S., Belo J.A.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 19-37; 60-90 AND 118-135, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    3. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiNDUS8_MOUSE
    AccessioniPrimary (citable) accession number: Q8K3J1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3