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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene

Ndufs8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi113 – 1131Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi116 – 1161Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi119 – 1191Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi123 – 1231Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi152 – 1521Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi155 – 1551Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi158 – 1581Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi162 – 1621Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

GO - Biological processi

  1. mitochondrial respiratory chain complex I assembly Source: MGI
  2. response to oxidative stress Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiREACT_278797. Respiratory electron transport.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-23kD
Short name:
CI-23kD
NADH-ubiquinone oxidoreductase 23 kDa subunit
Gene namesi
Name:Ndufs8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2385079. Ndufs8.

Subcellular locationi

  1. Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrial respiratory chain complex I Source: MGI
  2. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434MitochondrionBy similarityAdd
BLAST
Chaini35 – 212178NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialPRO_0000020014Add
BLAST

Proteomic databases

MaxQBiQ8K3J1.
PaxDbiQ8K3J1.
PRIDEiQ8K3J1.

2D gel databases

REPRODUCTION-2DPAGEQ8K3J1.

PTM databases

PhosphoSiteiQ8K3J1.

Expressioni

Gene expression databases

BgeeiQ8K3J1.
CleanExiMM_NDUFS8.
ExpressionAtlasiQ8K3J1. baseline and differential.
GenevestigatoriQ8K3J1.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.By similarity

Protein-protein interaction databases

BioGridi230440. 1 interaction.
IntActiQ8K3J1. 2 interactions.
MINTiMINT-1859976.

Structurei

3D structure databases

ProteinModelPortaliQ8K3J1.
SMRiQ8K3J1. Positions 66-191.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini104 – 133304Fe-4S ferredoxin-type 1Add
BLAST
Domaini143 – 172304Fe-4S ferredoxin-type 2Add
BLAST

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated
Contains 2 4Fe-4S ferredoxin-type domains.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiCOG1143.
GeneTreeiENSGT00390000003049.
HOGENOMiHOG000228289.
HOVERGENiHBG006547.
InParanoidiQ8K3J1.
KOiK03941.
OMAiDLFKCIY.
OrthoDBiEOG7J9VQT.
PhylomeDBiQ8K3J1.
TreeFamiTF105610.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K3J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRLSSSMLP RALAQAMRTG HLNGQSLHSS AVAATYKYVN KKEQESEVDM
60 70 80 90 100
KSATDNAARI LMWTELIRGL GMTLSYLFRE PATINYPFEK GPLSPRFRGE
110 120 130 140 150
HALRRYPSGE ERCIACKLCE AICPAQAITI EAEPRADGSR RTTRYDIDMT
160 170 180 190 200
KCIYCGFCQE ACPVDAIVEG PNFEFSTETH EELLYNKEKL LNNGDKWEAE
210
IAANIQADYL YR
Length:212
Mass (Da):24,038
Last modified:October 1, 2002 - v1
Checksum:i1E06E024EA7829CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY096002 Genomic DNA. Translation: AAM34451.1.
CCDSiCCDS29402.1.
PIRiPC7079.
RefSeqiNP_001258372.1. NM_001271443.1.
NP_001258373.1. NM_001271444.1.
NP_659119.2. NM_144870.5.
UniGeneiMm.44227.

Genome annotation databases

EnsembliENSMUST00000075092; ENSMUSP00000074600; ENSMUSG00000059734.
GeneIDi225887.
KEGGimmu:225887.
UCSCiuc008fxn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY096002 Genomic DNA. Translation: AAM34451.1.
CCDSiCCDS29402.1.
PIRiPC7079.
RefSeqiNP_001258372.1. NM_001271443.1.
NP_001258373.1. NM_001271444.1.
NP_659119.2. NM_144870.5.
UniGeneiMm.44227.

3D structure databases

ProteinModelPortaliQ8K3J1.
SMRiQ8K3J1. Positions 66-191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230440. 1 interaction.
IntActiQ8K3J1. 2 interactions.
MINTiMINT-1859976.

PTM databases

PhosphoSiteiQ8K3J1.

2D gel databases

REPRODUCTION-2DPAGEQ8K3J1.

Proteomic databases

MaxQBiQ8K3J1.
PaxDbiQ8K3J1.
PRIDEiQ8K3J1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075092; ENSMUSP00000074600; ENSMUSG00000059734.
GeneIDi225887.
KEGGimmu:225887.
UCSCiuc008fxn.1. mouse.

Organism-specific databases

CTDi4728.
MGIiMGI:2385079. Ndufs8.

Phylogenomic databases

eggNOGiCOG1143.
GeneTreeiENSGT00390000003049.
HOGENOMiHOG000228289.
HOVERGENiHBG006547.
InParanoidiQ8K3J1.
KOiK03941.
OMAiDLFKCIY.
OrthoDBiEOG7J9VQT.
PhylomeDBiQ8K3J1.
TreeFamiTF105610.

Enzyme and pathway databases

ReactomeiREACT_278797. Respiratory electron transport.

Miscellaneous databases

NextBioi377849.
PROiQ8K3J1.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K3J1.
CleanExiMM_NDUFS8.
ExpressionAtlasiQ8K3J1. baseline and differential.
GenevestigatoriQ8K3J1.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic organization of the mouse NDUFS8 gene encoding the NADH dehydrogenase:ubiquinone Fe-S protein 8."
    Lucas-Teixeira V.A., Marques S., Belo J.A.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 19-37; 60-90 AND 118-135, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiNDUS8_MOUSE
AccessioniPrimary (citable) accession number: Q8K3J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: October 1, 2002
Last modified: April 1, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.