Reviewed, UniProtKB/Swiss-Prot Q8K3J1 (NDUS8_MOUSE)

Names and origin

Protein names

Recommended name:
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    NADH-ubiquinone oxidoreductase 23 kDa subunit
    Complex I-23kD
      Short name=CI-23kD

Gene names

Name:

Ndufs8

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Cofactor	Binds 2 4Fe-4S clusters per subunit By similarity.
Subunit structure	Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the complex I 23 kDa subunit family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Domain	Repeat Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: MGI respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 34	34	Mitochondrion By similarity
	Chain	35 – 212	178	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial		PRO_0000020014
Regions
	Domain	104 – 133	30	4Fe-4S ferredoxin-type 1
	Domain	143 – 172	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	113	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	116	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	119	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	123	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	152	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	155	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	158	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	162	1	Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K3J1-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 1E06E024EA7829CD	FASTA	212	24,038
	10 20 30 40 50 60 MYRLSSSMLP RALAQAMRTG HLNGQSLHSS AVAATYKYVN KKEQESEVDM KSATDNAARI 70 80 90 100 110 120 LMWTELIRGL GMTLSYLFRE PATINYPFEK GPLSPRFRGE HALRRYPSGE ERCIACKLCE 130 140 150 160 170 180 AICPAQAITI EAEPRADGSR RTTRYDIDMT KCIYCGFCQE ACPVDAIVEG PNFEFSTETH 190 200 210 EELLYNKEKL LNNGDKWEAE IAANIQADYL YR

References

[1]	"Genomic organization of the mouse NDUFS8 gene encoding the NADH dehydrogenase:ubiquinone Fe-S protein 8." Lucas-Teixeira V.A., Marques S., Belo J.A. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 19-37; 60-90 AND 118-135, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY096002 Genomic DNA. Translation: AAM34451.1.
IPI	IPI00170093.
PIR	PC7079.
RefSeq	NP_659119.2.
UniGene	Mm.44227
3D structure databases
HSSP	HSSP built from PDB template 2FDN based on UniProtKB P00198.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8K3J1.
PTM databases
PhosphoSite	Q8K3J1.
2-D gel databases
REPRODUCTION-2DPAGE	Q8K3J1.
Proteomic databases
PRIDE	Q8K3J1.
Genome annotation databases
Ensembl	ENSMUST00000075092; ENSMUSP00000074600; ENSMUSG00000059734; Mus musculus. [Genome view]
GeneID	225887.
KEGG	mmu:225887.
NMPDR	fig|10090.3.peg.4372.
UCSC	uc008fxn.1. mouse.
Organism-specific databases
CTD	225887.
MGI	MGI:2385079. Ndufs8.
Phylogenomic databases
HOVERGEN	Q8K3J1.
OMA	QADFLYR.
Enzyme and pathway databases
BRENDA	1.6.5.3. 244. 1.6.99.3. 244.
Gene expression databases
ArrayExpress	Q8K3J1.
Bgee	Q8K3J1.
CleanEx	MM_NDUFS8.
Genevestigator	Q8K3J1.
GermOnline	ENSMUSG00000059734. Mus musculus.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR001450. 4Fe4S_Fe_S_bd_subgr. IPR017900. 4Fe4S_Fe_S_CS. IPR010226. NADH_quinone_OxRdtase_chainI. [Graphical view]
Pfam	PF00037. Fer4. 2 hits. [Graphical view]
TIGRFAMs	TIGR01971. NuoI. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	377849.
SOURCE	Search...

Entry information

Entry name

NDUS8_MOUSE

Accession

Primary (citable) accession number: Q8K3J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)