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Protein

DCC-interacting protein 13-alpha

Gene

Appl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein that interacts with proteins involved in different cellular signaling pathways. Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction. Involved in the regulation of the insulin receptor signaling pathway.By similarity
Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle

Enzyme and pathway databases

ReactomeiR-MMU-418889. Ligand-independent caspase activation via DCC.

Names & Taxonomyi

Protein namesi
Recommended name:
DCC-interacting protein 13-alpha
Short name:
Dip13-alpha
Alternative name(s):
Adapter protein containing PH domain, PTB domain and leucine zipper motif 1
Gene namesi
Name:Appl1
Synonyms:Dip13a, Kiaa1428Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1920243. Appl1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000799861 – 707DCC-interacting protein 13-alphaAdd BLAST707

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei399PhosphothreonineBy similarity1
Modified residuei401PhosphoserineBy similarity1
Modified residuei410Phosphoserine; by PKABy similarity1
Modified residuei691PhosphoserineCombined sources1
Modified residuei694PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation at Ser-410 by PKA severely impairs binding to OCRL.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8K3H0.
MaxQBiQ8K3H0.
PaxDbiQ8K3H0.
PeptideAtlasiQ8K3H0.
PRIDEiQ8K3H0.

PTM databases

iPTMnetiQ8K3H0.
PhosphoSitePlusiQ8K3H0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040760.
GenevisibleiQ8K3H0. MM.

Interactioni

Subunit structurei

Binds RAB5A/Rab5 through an N-terminal domain. This interaction is essential for its recruitment to endosomal membranes as well as its role in cell proliferation. Binds DCC and the catalytic domain of the inactive form of AKT2 through its PID domain. Binds PIK3CA and subunits of the NuRD/MeCP1 complex (By similarity). Interacts with OCRL and INPP5B. Interacts with NTRK2 (PubMed:21849472).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi215698. 4 interactors.
IntActiQ8K3H0. 5 interactors.
MINTiMINT-136655.
STRINGi10090.ENSMUSP00000042875.

Structurei

3D structure databases

ProteinModelPortaliQ8K3H0.
SMRiQ8K3H0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini277 – 375PHPROSITE-ProRule annotationAdd BLAST99
Domaini495 – 655PIDPROSITE-ProRule annotationAdd BLAST161

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 428Required for RAB5A bindingBy similarityAdd BLAST428

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili234 – 257Sequence analysisAdd BLAST24
Coiled coili620 – 670Sequence analysisAdd BLAST51

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi403 – 414F&HBy similarityAdd BLAST12

Domaini

Overexpression of an N-terminal domain (residues 1-319) or a C-terminal region (residues 273-707) has a proapoptotic effect.By similarity
The F&H motif, an approximately 12-13 amino-acid sequence centered around Phe and His residues, is essential for binding to OCRL and INPP5B.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0521. Eukaryota.
KOG3536. Eukaryota.
ENOG410ZISK. LUCA.
GeneTreeiENSGT00710000106752.
HOGENOMiHOG000285988.
HOVERGENiHBG051394.
InParanoidiQ8K3H0.
KOiK08733.
OMAiSDVETMQ.
OrthoDBiEOG091G08AV.
TreeFamiTF328669.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
2.30.29.30. 2 hits.
InterProiView protein in InterPro
IPR027267. AH/BAR-dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR006020. PTB/PI_dom.
PfamiView protein in Pfam
PF00169. PH. 1 hit.
PF00640. PID. 1 hit.
SMARTiView protein in SMART
SM00233. PH. 1 hit.
SM00462. PTB. 1 hit.
SUPFAMiSSF103657. SSF103657. 1 hit.
SSF50729. SSF50729. 2 hits.
PROSITEiView protein in PROSITE
PS50003. PH_DOMAIN. 1 hit.
PS01179. PID. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8K3H0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGIDKLPIE ETLEDSPQTR SLLGVFEEDA TAISNYMNQL YQAMHRIYDA
60 70 80 90 100
QNELSAATHL TSKLLKEYEK QRFPLGGDDE VMSSTLQQFS KVIDELSSCH
110 120 130 140 150
AVLSTQLADA MMFPISQFKE RDLKEILTLK EVFQIASNDH DAAINRYSRL
160 170 180 190 200
SKKRENDKVK YEVTEDVYTS RKKQHQTMMH YFCALNTLQY KKKIALLEPL
210 220 230 240 250
LGYMQAQISF FKMGSENLNG QLEEFLANIG TSVQNVRREM DGDVETMQQT
260 270 280 290 300
IEDLEVASDP LYLPDPDPTK FPINRNLTRK AGYLNARNKT GLVSSTWDRQ
310 320 330 340 350
FYFTQGGNLM SQARGDVAGG LAMDIDNCSV MAVDCEDRRY CFQITSFDGK
360 370 380 390 400
KSSILQAESK KDHEEWICTI NNISKQIYLS ENPEETAARV NQSALEAVTP
410 420 430 440 450
SPSFQQRHES LRPGGQSRPP TARTSSSGSL GSESTNLAAL SLDSLVAPDT
460 470 480 490 500
PIQFDIISPV CEDQPGQAKA FGQGGRRTNP FGESGGSTKS ETEDSILHQL
510 520 530 540 550
FIVRFLGSME VKSDDHPDVV YETMRQILAA RAIHNIFRMT ESHLLVTCDC
560 570 580 590 600
LKLIDPQTQV TRLTFPLPCV VLYATHQENK RLFGFVLRTS GGRSESNLSS
610 620 630 640 650
VCYIFESNNE GEKICDSVGL AKQIALHAEL DRRASEKQKE IERVKEKQQK
660 670 680 690 700
ELSKQKQIEK DLEEQSRLIA ASSRPNQAGS EGQLVLSSSQ SEESDLGEEG

KKRESEA
Length:707
Mass (Da):79,328
Last modified:October 1, 2002 - v1
Checksum:i7B0F4BF4B6247A7F
GO

Sequence cautioni

Q8K3H0: The sequence AAH19708 differs from that shown. Reason: Erroneous initiation.Curated
Q8K3H0: The sequence AAH19708 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37M → K in BAB28966 (PubMed:16141072).Curated1
Sequence conflicti117Q → P in BAB28966 (PubMed:16141072).Curated1
Sequence conflicti136A → T in BAB28966 (PubMed:16141072).Curated1
Sequence conflicti151S → P in BAB28966 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY113705 mRNA. Translation: AAM55531.1.
AK013715 mRNA. Translation: BAB28966.3.
AK033566 mRNA. Translation: BAC28364.1.
AK045438 mRNA. Translation: BAC32367.1.
AK049307 mRNA. Translation: BAC33672.2.
AK146356 mRNA. Translation: BAE27108.1.
BC019708 mRNA. Translation: AAH19708.1. Different initiation.
BC063751 mRNA. Translation: AAH63751.1.
AK173169 mRNA. Translation: BAD32447.1.
CCDSiCCDS26883.1.
RefSeqiNP_660256.1. NM_145221.2.
UniGeneiMm.202322.

Genome annotation databases

EnsembliENSMUST00000036570; ENSMUSP00000042875; ENSMUSG00000040760.
GeneIDi72993.
KEGGimmu:72993.
UCSCiuc007sth.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiDP13A_MOUSE
AccessioniPrimary (citable) accession number: Q8K3H0
Secondary accession number(s): Q3UJP7
, Q69ZJ9, Q8BWZ8, Q8VCJ8, Q9CUW4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: October 1, 2002
Last modified: October 25, 2017
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot