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Protein

Inactive serine/threonine-protein kinase VRK3

Gene

Vrk3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inactive kinase that suppresses ERK activity by promoting phosphatase activity of DUSP3 which specifically dephosphorylates and inactivates ERK in the nucleus.2 Publications

GO - Molecular functioni

  • ATP binding Source: InterPro
  • protein phosphatase binding Source: MGI
  • protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

Keywordsi

LigandNucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-202670. ERKs are inactivated.

Names & Taxonomyi

Protein namesi
Recommended name:
Inactive serine/threonine-protein kinase VRK3
Alternative name(s):
Serine/threonine-protein pseudokinase VRK3
Vaccinia-related kinase 3
Gene namesi
Name:Vrk3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2182465. Vrk3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000868091 – 453Inactive serine/threonine-protein kinase VRK3Add BLAST453

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54PhosphoserineBy similarity1
Modified residuei55PhosphoserineBy similarity1
Modified residuei59PhosphoserineCombined sources1
Modified residuei82PhosphoserineBy similarity1
Modified residuei83PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8K3G5.
MaxQBiQ8K3G5.
PaxDbiQ8K3G5.
PRIDEiQ8K3G5.

PTM databases

iPTMnetiQ8K3G5.
PhosphoSitePlusiQ8K3G5.

Expressioni

Tissue specificityi

Expressed in liver, kidney, muscle, thymus, and bone marrow. Weakly expressed in spleen.1 Publication

Developmental stagei

Weakly expressed in embryo compared to VRK1 and VRK3. Expressed from E10.5 to E13.5 in developing liver and then decreases. It increases again from E17.5 and remains thereafter. Highly expressed in hematopoietic embryonic tissues from E10.5 to E14.5. Strongly expressed in the yolk-sac.1 Publication

Gene expression databases

BgeeiENSMUSG00000002205.
CleanExiMM_VRK3.
ExpressionAtlasiQ8K3G5. baseline and differential.
GenevisibleiQ8K3G5. MM.

Interactioni

Subunit structurei

Interacts with DUSP3. Interacts with RAN (By similarity).By similarity

GO - Molecular functioni

  • protein phosphatase binding Source: MGI

Protein-protein interaction databases

BioGridi221691. 2 interactors.
IntActiQ8K3G5. 1 interactor.
STRINGi10090.ENSMUSP00000002275.

Structurei

3D structure databases

ProteinModelPortaliQ8K3G5.
SMRiQ8K3G5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini125 – 436Protein kinasePROSITE-ProRule annotationAdd BLAST312

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi49 – 64Nuclear localization signal1 PublicationAdd BLAST16

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1164. Eukaryota.
ENOG410XPGP. LUCA.
GeneTreeiENSGT00760000119040.
HOGENOMiHOG000293191.
HOVERGENiHBG047756.
InParanoidiQ8K3G5.
KOiK08816.
OMAiYEEKPPY.
OrthoDBiEOG091G05V5.
PhylomeDBiQ8K3G5.
TreeFamiTF106473.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS50011. PROTEIN_KINASE_DOM. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8K3G5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISFCPVCGK SVKVSFKFCP YCGKALPVEE DGGTQSAVTP HVSSVPGSRR
60 70 80 90 100
DLNSSFETSP KKVKCSHTVT SLPLSRHSDC DSSGSDNTLT SPDRATGTRS
110 120 130 140 150
RPLTPKGSPL SNRQSPQTLK RTRVTTSLQA LATGTELTDQ NGKHWTLGAL
160 170 180 190 200
QIRDDQGILY EAEPTSAVPS ESRTQKWRFS LKLDSKDGRL FNEQNFFQRV
210 220 230 240 250
AKPLQVNKWK KQFLLPLLAI PTCIGFGIHQ DKYRFLVFPS LGRSLQSALD
260 270 280 290 300
DNPKHVVSER CVLQVACRLL DALEYLHENE YVHGNLTAEN VFVNPEDLSQ
310 320 330 340 350
VTLVGYGFTY RYCPGGKHVA YKEGSRSPHD GDLEFISMDL HKGCGPSRRS
360 370 380 390 400
DLQTLGYCML KWLYGSLPWT NCLPNTEKIT RQKQKYLDSP ERLVGLCGRW
410 420 430 440 450
NKASETLREY LKVVMALNYE EKPPYATLRN SLEALLQDMR VSPYDPLDLQ

MVP
Length:453
Mass (Da):50,830
Last modified:October 10, 2003 - v2
Checksum:iB882A3F60802C432
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44S → P in AAM74471 (PubMed:11986327).Curated1
Sequence conflicti168V → I in AAM74471 (PubMed:11986327).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY115107 Genomic DNA. Translation: AAM74471.1.
BC010473 mRNA. Translation: AAH10473.1.
BC024782 mRNA. Translation: AAH24782.1.
BC024839 mRNA. Translation: AAH24839.1.
BC034196 mRNA. Translation: AAH34196.1.
CCDSiCCDS21214.1.
RefSeqiNP_598706.1. NM_133945.1.
XP_006540601.1. XM_006540538.3.
UniGeneiMm.28816.

Genome annotation databases

EnsembliENSMUST00000002275; ENSMUSP00000002275; ENSMUSG00000002205.
GeneIDi101568.
KEGGimmu:101568.
UCSCiuc009gqr.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiVRK3_MOUSE
AccessioniPrimary (citable) accession number: Q8K3G5
Secondary accession number(s): Q921W6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 10, 2003
Last modified: October 25, 2017
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Inactive as a kinase due to its inability to bind ATP.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families