Q8K3F7 (TDH_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-threonine 3-dehydrogenase, mitochondrial
EC=1.1.1.103

Gene names

Name:

Tdh

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH. UniProtKB Q8MIR0
Pathway	Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2.
Subcellular location	Mitochondrion By similarity UniProtKB Q8MIR0.
Sequence similarities	Belongs to the sugar epimerase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from direct assay. Source: MGI
Molecular function	L-threonine 3-dehydrogenase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential UniProtKB Q8MIR0
	Chain	? – 373		L-threonine 3-dehydrogenase, mitochondrial		PRO_0000298784
Regions
	Nucleotide binding	9 – 46	38	NAD By similarity UniProtKB Q8R059
Sites
	Active site	195	1	Proton acceptor By similarity UniProtKB Q8R059
Experimental info
	Sequence conflict	82	1	D → N in AAH58860. Ref.3
	Sequence conflict	214	1	G → V in AAF61395. Ref.1
	Sequence conflict	232	1	P → R in AAF61395. Ref.1
	Sequence conflict	318	1	Y → H in AAH58860. Ref.3
	Sequence conflict	361	1	N → S in AAF61395. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8K3F7 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 6A416A079A7EC381	FASTA	373	41,462
	10 20 30 40 50 60 MLFLGMLKQV VNGTAQSKAS SCRKLVLPLK FLGTSQHRIP ADANFHSTSI SEAEPPRVLI 70 80 90 100 110 120 TGGLGQLGVG LANLLRKRFG KDNVILSDIR KPPAHVFHSG PFVYANILDY KSLREIVVNH 130 140 150 160 170 180 RISWLFHYSA LLSAVGEANV SLARDVNITG LHNVLDVAAE YNVRLFVPST IGAFGPTSPR 190 200 210 220 230 240 NPAPDLCIQR PRTIYGVSKV HTELMGEYYY YRYGLDFRCL RYPGIISADS QPGGGTTDYA 250 260 270 280 290 300 VQIFHAAAKN GTFECNLEAG TRLPMMYISD CLRATLEVME APAERLSMRT YNISAMSFTP 310 320 330 340 350 360 EELAQALRKH APDFQITYCV DPLRQAIAES WPMILDDSNA RKDWGWKHDF DLPELVATML 370 NFHGVSTRVA QVN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and tissue distribution of mammalian L-threonine 3-dehydrogenases." Edgar A.J. BMC Biochem. 3:19-19(2002) [PubMed: 12097150] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Liver.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF134346 mRNA. Translation: AAF61395.2. AY116662 mRNA. Translation: AAM51557.1. AK077571 mRNA. Translation: BAC36870.1. BC058860 mRNA. Translation: AAH58860.1.
IPI	IPI00331660.
RefSeq	NP_067455.5. NM_021480.5.
UniGene	Mm.354229.
3D structure databases
ProteinModelPortal	Q8K3F7.
SMR	Q8K3F7. Positions 55-363.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8K3F7.
Proteomic databases
PRIDE	Q8K3F7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022522; ENSMUSP00000022522; ENSMUSG00000021953.
GeneID	58865.
KEGG	mmu:58865.
UCSC	uc007uhs.1. mouse.
Organism-specific databases
CTD	157739.
MGI	MGI:1926231. Tdh.
Phylogenomic databases
eggNOG	roNOG14653.
GeneTree	ENSGT00390000014037.
HOGENOM	HBG304215.
HOVERGEN	HBG062086.
InParanoid	Q8K3F7.
OMA	FRCLRFP.
OrthoDB	EOG4B2SXX.
PhylomeDB	Q8K3F7.
Enzyme and pathway databases
BRENDA	1.1.1.103. 3474.
Gene expression databases
ArrayExpress	Q8K3F7.
Bgee	Q8K3F7.
CleanEx	MM_TDH.
Genevestigator	Q8K3F7.
Family and domain databases
InterPro	IPR001509. Epimerase_deHydtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00060.
Pfam	PF01370. Epimerase. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	314424.
SOURCE	Search...

Entry information

Entry name

TDH_MOUSE

Accession

Primary (citable) accession number: Q8K3F7
Secondary accession number(s): Q6PD91, Q9JLU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program