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Protein

Matrix metalloproteinase-21

Gene

Mmp21

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a specialized role in the generation of left-right asymmetry during embryogenesis (PubMed:26437028). May act as a negative regulator of the NOTCH-signaling pathway. Cleaves alpha-1-antitrypsin (By similarity).By similarity1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
  • Ca2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi112Zinc; in inhibited formBy similarity1
Metal bindingi282Zinc; catalyticPROSITE-ProRule annotation1
Active sitei283PROSITE-ProRule annotation1
Metal bindingi286Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi292Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • coronary vasculature development Source: MGI
  • determination of heart left/right asymmetry Source: UniProtKB
  • determination of left/right symmetry Source: UniProtKB
  • hematopoietic progenitor cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM10.026.

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix metalloproteinase-21 (EC:3.4.24.-)
Short name:
MMP-21
Gene namesi
Name:Mmp21
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2664387. Mmp21.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi225I → T: Embryos have congenital heart defects with transposition of the great artery, 61% exhibiting situs inversus or heterotaxy and 39% exhibiting situs solitus. This mutation corresponds to the pathological variant 'T-226' identified in humans. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000043708925 – 143By similarityAdd BLAST119
ChainiPRO_0000028841144 – 568Matrix metalloproteinase-21Add BLAST425

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi328 ↔ 559By similarity
Glycosylationi371N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ8K3F2.
PRIDEiQ8K3F2.

PTM databases

PhosphoSitePlusiQ8K3F2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030981.
CleanExiMM_MMP21.
ExpressionAtlasiQ8K3F2. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033278.

Structurei

3D structure databases

ProteinModelPortaliQ8K3F2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati329 – 388Hemopexin 1Add BLAST60
Repeati390 – 446Hemopexin 2Add BLAST57
Repeati447 – 495Hemopexin 3Add BLAST49
Repeati502 – 558Hemopexin 4Add BLAST57

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi110 – 117Cysteine switchBy similarity8

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated
Contains 4 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00860000133713.
HOGENOMiHOG000113608.
HOVERGENiHBG052483.
InParanoidiQ8K3F2.
KOiK08000.
OMAiEFAHAWR.
OrthoDBiEOG091G0C9G.
PhylomeDBiQ8K3F2.
TreeFamiTF315428.

Family and domain databases

CDDicd00094. HX. 1 hit.
cd04278. ZnMc_MMP. 1 hit.
Gene3Di1.10.101.10. 1 hit.
2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamiPF00045. Hemopexin. 3 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K3F2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAASVLRLT LPLCWLVAPQ PTQPERLFHS RDRSDLEPSP LSQAKPIADL
60 70 80 90 100
HDAQSFLLKY GWSEIPSPKE SAGVPVGFTL AQAVRRFQKA NRLPASGELD
110 120 130 140 150
SPTLAAMNKP RCGVPDTRLP PRAALPTPPA LLTSLGLRPR ARQKRFLQML
160 170 180 190 200
FPKPDGQQED ASDTGASRAF SKKTLTWRLV GDAYSSQLSG DEQRYIFRLA
210 220 230 240 250
FRMWSEVTPL DFREDRTAPG TMVDIKLGFG RGRHLGCPRV FDGSGQEFAH
260 270 280 290 300
AWRLGEIHFD DDEHFTPLSS DTGISLLKVA VHEIGHVLGL PHTYRVGSIM
310 320 330 340 350
QPNYIPQEPA FELDWADRKA IQRLYGSCKG SFDTVFDWIR KERNQYGEVR
360 370 380 390 400
VRFNTYFFRN SWYWLYENRN NRTRYGDPLQ ILTGWRGIPT QSIDAYVHVW
410 420 430 440 450
SWGKDERYFF KGSQYWRYDS ENDQAHTEDE QGRSYPKLIS EGFPGIPSPL
460 470 480 490 500
DTAFYDRRQQ LIYFFKESLV FAFDVNRNQV LNSYPKKMSQ VFPAIMPQNH
510 520 530 540 550
PFRNLDSAYY SYAHNSIFFF KGNSYWKVVS DKDKQQNTRL PLNGLFPKKP
560
VSEKWFDVCD VHTSTLNM
Length:568
Mass (Da):65,454
Last modified:October 1, 2002 - v1
Checksum:iEDDBDDD9B6E00546
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY124569 mRNA. Translation: AAM94032.1.
AF507967 mRNA. Translation: AAN09805.1.
CCDSiCCDS21933.1.
RefSeqiNP_694423.1. NM_152944.1.
UniGeneiMm.222952.

Genome annotation databases

EnsembliENSMUST00000033278; ENSMUSP00000033278; ENSMUSG00000030981.
GeneIDi214766.
KEGGimmu:214766.
UCSCiuc009kdd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY124569 mRNA. Translation: AAM94032.1.
AF507967 mRNA. Translation: AAN09805.1.
CCDSiCCDS21933.1.
RefSeqiNP_694423.1. NM_152944.1.
UniGeneiMm.222952.

3D structure databases

ProteinModelPortaliQ8K3F2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033278.

Protein family/group databases

MEROPSiM10.026.

PTM databases

PhosphoSitePlusiQ8K3F2.

Proteomic databases

PaxDbiQ8K3F2.
PRIDEiQ8K3F2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033278; ENSMUSP00000033278; ENSMUSG00000030981.
GeneIDi214766.
KEGGimmu:214766.
UCSCiuc009kdd.1. mouse.

Organism-specific databases

CTDi118856.
MGIiMGI:2664387. Mmp21.

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00860000133713.
HOGENOMiHOG000113608.
HOVERGENiHBG052483.
InParanoidiQ8K3F2.
KOiK08000.
OMAiEFAHAWR.
OrthoDBiEOG091G0C9G.
PhylomeDBiQ8K3F2.
TreeFamiTF315428.

Miscellaneous databases

PROiQ8K3F2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030981.
CleanExiMM_MMP21.
ExpressionAtlasiQ8K3F2. baseline and differential.

Family and domain databases

CDDicd00094. HX. 1 hit.
cd04278. ZnMc_MMP. 1 hit.
Gene3Di1.10.101.10. 1 hit.
2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamiPF00045. Hemopexin. 3 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMMP21_MOUSE
AccessioniPrimary (citable) accession number: Q8K3F2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.