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Protein

F-box/SPRY domain-containing protein 1

Gene

Fbxo45

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of E3 ubiquitin ligase complexes. Required for normal neuromuscular synaptogenesis, axon pathfinding and neuronal migration. Plays a role in the regulation of neurotransmission at mature neurons (By similarity). May control synaptic activity by controlling UNC13A via ubiquitin dependent pathway. Specifically recognizes TP73, promoting its ubiquitination and degradation (By similarity).By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • anterior commissure morphogenesis Source: MGI
  • cellular response to DNA damage stimulus Source: MGI
  • cerebral cortex radially oriented cell migration Source: MGI
  • cerebral cortex tangential migration Source: MGI
  • corticospinal tract morphogenesis Source: MGI
  • innervation Source: MGI
  • neuron migration Source: MGI
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
  • protein ubiquitination Source: UniProtKB
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: MGI
  • synapse assembly involved in innervation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Neurogenesis, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box/SPRY domain-containing protein 1
Alternative name(s):
F-box only protein 45
Short name:
mFbxo45
Gene namesi
Name:Fbxo45Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:2447775. Fbxo45.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • postsynaptic density Source: UniProtKB
  • postsynaptic membrane Source: UniProtKB-SubCell
  • presynaptic membrane Source: UniProtKB-SubCell
  • synapse Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Homozygous animals die soon after birth due to respiratory distress. Embryos show abnormal innervation of the diaphragm, impaired synapse formation at neuromuscular junctions, and aberrant development of axon fiber tracts in the brain.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi42R → E: Allows binding to CUL1 and RBX1 and formation of an authentic SCF (SKP1-CUL1-F-box protein) complex. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001199472 – 286F-box/SPRY domain-containing protein 1Add BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8K3B1.
MaxQBiQ8K3B1.
PaxDbiQ8K3B1.
PeptideAtlasiQ8K3B1.
PRIDEiQ8K3B1.

PTM databases

PhosphoSitePlusiQ8K3B1.

Expressioni

Tissue specificityi

Expressed speciffically in the central nervous system, including cerebellum, medulla oblongata, olfactory bulb, hippocampus, cortex and brain stem.2 Publications

Gene expression databases

BgeeiENSMUSG00000035764.
CleanExiMM_FBXO45.
GenevisibleiQ8K3B1. MM.

Interactioni

Subunit structurei

Forms a complex with MYCBP2 and SKP1 but does not appear to form an authentic SCF (SKP1-CUL1-F-box protein) complex. Interacts (via B30.2/SPRY domain) with TP73 (via SAM domain); leading to its ubiquitination and degradation (By similarity). Interacts (via SRY domain) with UNC13A; leading to its degradation by the proteasome.By similarity2 Publications

Protein-protein interaction databases

BioGridi234572. 2 interactors.
STRINGi10090.ENSMUSP00000040168.

Structurei

3D structure databases

ProteinModelPortaliQ8K3B1.
SMRiQ8K3B1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 82F-boxPROSITE-ProRule annotationAdd BLAST50
Domaini92 – 284B30.2/SPRYPROSITE-ProRule annotationAdd BLAST193

Sequence similaritiesi

Belongs to the FBXO45/Fsn family.Curated
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 F-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3953. Eukaryota.
ENOG410XQC1. LUCA.
GeneTreeiENSGT00390000009402.
HOGENOMiHOG000230524.
HOVERGENiHBG056500.
InParanoidiQ8K3B1.
KOiK10319.
OMAiHAWEIWW.
OrthoDBiEOG091G0DF0.
PhylomeDBiQ8K3B1.
TreeFamiTF312822.

Family and domain databases

InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR001810. F-box_dom.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50181. FBOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K3B1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPGPGAGA ASGGASGGGA GAGGGASAGS GSSGVGGRLP SRVLELVFSY
60 70 80 90 100
LELSELRSCA LVCKHWYRCL HGDENSEVWR SLCARSLAEE ALRTDILCNL
110 120 130 140 150
PSYKAKVRAF QHAFSTNDCS RNVYIKKNGF TLHRNPIAQS TDGARTKIGF
160 170 180 190 200
SEGRHAWEVW WEGPLGTVAV IGIATKRAPM QCQGYVALLG SDDQSWGWNL
210 220 230 240 250
VDNNLLHNGE VNGSFPQCNN APKYQIGERI RVILDMEDKT LAFERGYEFL
260 270 280
GVAFRGLPKA CLYPAVSAVY GNTEVTLVYL GKPLDG
Length:286
Mass (Da):30,605
Last modified:October 25, 2005 - v2
Checksum:i78589911B5DA31B7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27S → C in AAH26799 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC026799 mRNA. Translation: AAH26799.1.
BC088738 mRNA. Translation: AAH88738.1.
BC098203 mRNA. Translation: AAH98203.1.
CCDSiCCDS28117.1.
RefSeqiNP_775615.2. NM_173439.2.
UniGeneiMm.256137.

Genome annotation databases

EnsembliENSMUST00000042732; ENSMUSP00000040168; ENSMUSG00000035764.
GeneIDi268882.
KEGGimmu:268882.
UCSCiuc007yyl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC026799 mRNA. Translation: AAH26799.1.
BC088738 mRNA. Translation: AAH88738.1.
BC098203 mRNA. Translation: AAH98203.1.
CCDSiCCDS28117.1.
RefSeqiNP_775615.2. NM_173439.2.
UniGeneiMm.256137.

3D structure databases

ProteinModelPortaliQ8K3B1.
SMRiQ8K3B1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234572. 2 interactors.
STRINGi10090.ENSMUSP00000040168.

PTM databases

PhosphoSitePlusiQ8K3B1.

Proteomic databases

EPDiQ8K3B1.
MaxQBiQ8K3B1.
PaxDbiQ8K3B1.
PeptideAtlasiQ8K3B1.
PRIDEiQ8K3B1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042732; ENSMUSP00000040168; ENSMUSG00000035764.
GeneIDi268882.
KEGGimmu:268882.
UCSCiuc007yyl.1. mouse.

Organism-specific databases

CTDi200933.
MGIiMGI:2447775. Fbxo45.

Phylogenomic databases

eggNOGiKOG3953. Eukaryota.
ENOG410XQC1. LUCA.
GeneTreeiENSGT00390000009402.
HOGENOMiHOG000230524.
HOVERGENiHBG056500.
InParanoidiQ8K3B1.
KOiK10319.
OMAiHAWEIWW.
OrthoDBiEOG091G0DF0.
PhylomeDBiQ8K3B1.
TreeFamiTF312822.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ8K3B1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035764.
CleanExiMM_FBXO45.
GenevisibleiQ8K3B1. MM.

Family and domain databases

InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR001810. F-box_dom.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFBSP1_MOUSE
AccessioniPrimary (citable) accession number: Q8K3B1
Secondary accession number(s): Q5M7B1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: November 2, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.