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Q8K387 (UBP45_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 45

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 45
Ubiquitin thioesterase 45
Ubiquitin-specific-processing protease 45
Gene names
Name:Usp45
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length813 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Sequence caution

The sequence BAC29631.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Coding sequence diversityAlternative splicing
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8K387-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 2 (identifier: Q8K387-2)

The sequence of this isoform differs from the canonical sequence as follows:
     390-437: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 813813Ubiquitin carboxyl-terminal hydrolase 45
PRO_0000280562

Regions

Domain191 – 812622USP
Zinc finger60 – 13677UBP-type

Sites

Active site2001Nucleophile By similarity
Active site7451Proton acceptor By similarity

Natural variations

Alternative sequence390 – 43748Missing in isoform 2.
VSP_023794

Experimental info

Sequence conflict5951R → G in BAC40129. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: ED1DD9B9F007B178

FASTA81390,361
        10         20         30         40         50         60 
MRVKDPSKDL PEKGKRNKRP LLPHDEDSSD DIAVGLTCQH VSYAVSVNHV KKAVAESLWS 

        70         80         90        100        110        120 
VCSECLKERR FCDGQPVLPA DVWLCLKCGL QGCGKNSESQ HSLRHFKSSG TESHCVVISL 

       130        140        150        160        170        180 
STWVIWCYEC NEKLSTHCNK KVLAQIVDFL QKHAFKTQTG AFSRIIKLCE EKREAGEIKK 

       190        200        210        220        230        240 
GKKGCTVPSV KGITNLGNTC FFNAVIQNLA QTYILFELMN EIKEDGTKFK ISLSSAPQLE 

       250        260        270        280        290        300 
PLVVELSSPG PLTSALFLFL HSMKEAEKGP LSPKVLFNQL CQKAPRFKGF QQQDSQELLH 

       310        320        330        340        350        360 
HLLDAVRTEE TKRIQASILK AFNNPTTKTA DDETRKKVKA YGKEGVKMNF IDRIFIGELT 

       370        380        390        400        410        420 
STVMCEECAN ISTMKDPFID ISLPIIEERV SKPVLLGKMS KCRSLQETDQ DHNKGTVTVG 

       430        440        450        460        470        480 
NAHQPRASRK HSSPNDKNQL SHDRKHLRKW PSEEEKTVVT HPKNDNLEAS PPASTLSTEA 

       490        500        510        520        530        540 
SLNESLTDGS ERDASLESSV DADSEASEPE IASKQPVLLR SRGDSCGHAE QHPHLPLASE 

       550        560        570        580        590        600 
LPQAKETHGG EEEMAEAIAE LHLSGTVTGN RDFHREKQPL NVPNNLCFSE GKHTRLHSAQ 

       610        620        630        640        650        660 
NAFQTLSQSY VTTSKECSVQ SCLYQFTSME LLMGNNKLLC EDCTEKRRKC HKETSSAEKK 

       670        680        690        700        710        720 
AGGVYTNARK QLLISAVPAI LILHLKRFHQ AGLSLRKVNR HVDFPLTLDL APFCAATCKN 

       730        740        750        760        770        780 
ISVGEKVLYG LYGIVEHSGS MRGGHYTAYV KVRVPSRKLS ECITGRKTAA GLKEPDGELG 

       790        800        810 
GHWVHVSDTY VQVVPESRAL SAQAYLLFYE RIL 

« Hide

Isoform 2 [UniParc].

Checksum: 08254CA4B7DEE012
Show »

FASTA76585,110

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J and NOD.
Tissue: Thymus and Vagina.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK036903 mRNA. Translation: BAC29631.1. Different initiation.
AK088073 mRNA. Translation: BAC40129.1.
AL772187 Genomic DNA. Translation: CAM22881.1.
BC027768 mRNA. Translation: AAH27768.1.
RefSeqNP_690038.1. NM_152825.2.
XP_006538424.1. XM_006538361.1.
XP_006538426.1. XM_006538363.1.
UniGeneMm.154306.

3D structure databases

ProteinModelPortalQ8K387.
SMRQ8K387. Positions 38-389, 602-810.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid218782. 3 interactions.

Protein family/group databases

MEROPSC19.064.

PTM databases

PhosphoSiteQ8K387.

Proteomic databases

PaxDbQ8K387.
PRIDEQ8K387.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000040429; ENSMUSP00000048324; ENSMUSG00000040455. [Q8K387-2]
ENSMUST00000065111; ENSMUSP00000067109; ENSMUSG00000040455. [Q8K387-1]
ENSMUST00000108232; ENSMUSP00000103867; ENSMUSG00000040455. [Q8K387-1]
GeneID77593.
KEGGmmu:77593.
UCSCuc008sda.1. mouse. [Q8K387-1]
uc008sdc.1. mouse. [Q8K387-2]

Organism-specific databases

CTD85015.
MGIMGI:101850. Usp45.

Phylogenomic databases

eggNOGCOG5560.
GeneTreeENSGT00750000117419.
HOGENOMHOG000154755.
HOVERGENHBG062704.
InParanoidQ8K387.
KOK11844.
OMANNLCFSE.
OrthoDBEOG7J9VNZ.
PhylomeDBQ8K387.
TreeFamTF326075.

Gene expression databases

ArrayExpressQ8K387.
BgeeQ8K387.
CleanExMM_USP45.
GenevestigatorQ8K387.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio347172.
PROQ8K387.
SOURCESearch...

Entry information

Entry nameUBP45_MOUSE
AccessionPrimary (citable) accession number: Q8K387
Secondary accession number(s): A2AJT1, Q8BU19, Q8BZ19
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot