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Q8K387

- UBP45_MOUSE

UniProt

Q8K387 - UBP45_MOUSE

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Protein
Ubiquitin carboxyl-terminal hydrolase 45
Gene
Usp45
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei200 – 2001Nucleophile By similarity
Active sitei745 – 7451Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri60 – 13677UBP-type
Add
BLAST

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. ubiquitinyl hydrolase activity Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.064.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 45 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 45
Ubiquitin thioesterase 45
Ubiquitin-specific-processing protease 45
Gene namesi
Name:Usp45
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:101850. Usp45.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 813813Ubiquitin carboxyl-terminal hydrolase 45
PRO_0000280562Add
BLAST

Proteomic databases

PaxDbiQ8K387.
PRIDEiQ8K387.

PTM databases

PhosphoSiteiQ8K387.

Expressioni

Gene expression databases

ArrayExpressiQ8K387.
BgeeiQ8K387.
CleanExiMM_USP45.
GenevestigatoriQ8K387.

Interactioni

Protein-protein interaction databases

BioGridi218782. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8K387.
SMRiQ8K387. Positions 38-389, 602-810.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini191 – 812622USP
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 1 USP domain.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5560.
GeneTreeiENSGT00750000117419.
HOGENOMiHOG000154755.
HOVERGENiHBG062704.
InParanoidiQ8K387.
KOiK11844.
OMAiECEHISA.
OrthoDBiEOG7J9VNZ.
PhylomeDBiQ8K387.
TreeFamiTF326075.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8K387-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MRVKDPSKDL PEKGKRNKRP LLPHDEDSSD DIAVGLTCQH VSYAVSVNHV    50
KKAVAESLWS VCSECLKERR FCDGQPVLPA DVWLCLKCGL QGCGKNSESQ 100
HSLRHFKSSG TESHCVVISL STWVIWCYEC NEKLSTHCNK KVLAQIVDFL 150
QKHAFKTQTG AFSRIIKLCE EKREAGEIKK GKKGCTVPSV KGITNLGNTC 200
FFNAVIQNLA QTYILFELMN EIKEDGTKFK ISLSSAPQLE PLVVELSSPG 250
PLTSALFLFL HSMKEAEKGP LSPKVLFNQL CQKAPRFKGF QQQDSQELLH 300
HLLDAVRTEE TKRIQASILK AFNNPTTKTA DDETRKKVKA YGKEGVKMNF 350
IDRIFIGELT STVMCEECAN ISTMKDPFID ISLPIIEERV SKPVLLGKMS 400
KCRSLQETDQ DHNKGTVTVG NAHQPRASRK HSSPNDKNQL SHDRKHLRKW 450
PSEEEKTVVT HPKNDNLEAS PPASTLSTEA SLNESLTDGS ERDASLESSV 500
DADSEASEPE IASKQPVLLR SRGDSCGHAE QHPHLPLASE LPQAKETHGG 550
EEEMAEAIAE LHLSGTVTGN RDFHREKQPL NVPNNLCFSE GKHTRLHSAQ 600
NAFQTLSQSY VTTSKECSVQ SCLYQFTSME LLMGNNKLLC EDCTEKRRKC 650
HKETSSAEKK AGGVYTNARK QLLISAVPAI LILHLKRFHQ AGLSLRKVNR 700
HVDFPLTLDL APFCAATCKN ISVGEKVLYG LYGIVEHSGS MRGGHYTAYV 750
KVRVPSRKLS ECITGRKTAA GLKEPDGELG GHWVHVSDTY VQVVPESRAL 800
SAQAYLLFYE RIL 813

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Length:813
Mass (Da):90,361
Last modified:October 1, 2002 - v1
Checksum:iED1DD9B9F007B178
GO
Isoform 2 (identifier: Q8K387-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     390-437: Missing.

Note: No experimental confirmation available.

Show »
Length:765
Mass (Da):85,110
Checksum:i08254CA4B7DEE012
GO

Sequence cautioni

The sequence BAC29631.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei390 – 43748Missing in isoform 2.
VSP_023794Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti595 – 5951R → G in BAC40129. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK036903 mRNA. Translation: BAC29631.1. Different initiation.
AK088073 mRNA. Translation: BAC40129.1.
AL772187 Genomic DNA. Translation: CAM22881.1.
BC027768 mRNA. Translation: AAH27768.1.
CCDSiCCDS38700.1. [Q8K387-1]
CCDS71351.1. [Q8K387-2]
RefSeqiNP_001277354.1. NM_001290425.1. [Q8K387-2]
NP_690038.1. NM_152825.2. [Q8K387-1]
XP_006538424.1. XM_006538361.1. [Q8K387-1]
UniGeneiMm.154306.

Genome annotation databases

EnsembliENSMUST00000040429; ENSMUSP00000048324; ENSMUSG00000040455. [Q8K387-2]
ENSMUST00000065111; ENSMUSP00000067109; ENSMUSG00000040455. [Q8K387-1]
ENSMUST00000108232; ENSMUSP00000103867; ENSMUSG00000040455. [Q8K387-1]
GeneIDi77593.
KEGGimmu:77593.
UCSCiuc008sda.1. mouse. [Q8K387-1]
uc008sdc.1. mouse. [Q8K387-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK036903 mRNA. Translation: BAC29631.1 . Different initiation.
AK088073 mRNA. Translation: BAC40129.1 .
AL772187 Genomic DNA. Translation: CAM22881.1 .
BC027768 mRNA. Translation: AAH27768.1 .
CCDSi CCDS38700.1. [Q8K387-1 ]
CCDS71351.1. [Q8K387-2 ]
RefSeqi NP_001277354.1. NM_001290425.1. [Q8K387-2 ]
NP_690038.1. NM_152825.2. [Q8K387-1 ]
XP_006538424.1. XM_006538361.1. [Q8K387-1 ]
UniGenei Mm.154306.

3D structure databases

ProteinModelPortali Q8K387.
SMRi Q8K387. Positions 38-389, 602-810.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 218782. 3 interactions.

Protein family/group databases

MEROPSi C19.064.

PTM databases

PhosphoSitei Q8K387.

Proteomic databases

PaxDbi Q8K387.
PRIDEi Q8K387.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000040429 ; ENSMUSP00000048324 ; ENSMUSG00000040455 . [Q8K387-2 ]
ENSMUST00000065111 ; ENSMUSP00000067109 ; ENSMUSG00000040455 . [Q8K387-1 ]
ENSMUST00000108232 ; ENSMUSP00000103867 ; ENSMUSG00000040455 . [Q8K387-1 ]
GeneIDi 77593.
KEGGi mmu:77593.
UCSCi uc008sda.1. mouse. [Q8K387-1 ]
uc008sdc.1. mouse. [Q8K387-2 ]

Organism-specific databases

CTDi 85015.
MGIi MGI:101850. Usp45.

Phylogenomic databases

eggNOGi COG5560.
GeneTreei ENSGT00750000117419.
HOGENOMi HOG000154755.
HOVERGENi HBG062704.
InParanoidi Q8K387.
KOi K11844.
OMAi ECEHISA.
OrthoDBi EOG7J9VNZ.
PhylomeDBi Q8K387.
TreeFami TF326075.

Miscellaneous databases

NextBioi 347172.
PROi Q8K387.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8K387.
Bgeei Q8K387.
CleanExi MM_USP45.
Genevestigatori Q8K387.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J and NOD.
    Tissue: Thymus and Vagina.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiUBP45_MOUSE
AccessioniPrimary (citable) accession number: Q8K387
Secondary accession number(s): A2AJT1, Q8BU19, Q8BZ19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: October 1, 2002
Last modified: September 3, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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