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Q8K387

- UBP45_MOUSE

UniProt

Q8K387 - UBP45_MOUSE

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Protein

Ubiquitin carboxyl-terminal hydrolase 45

Gene

Usp45

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei200 – 2001NucleophilePROSITE-ProRule annotation
Active sitei745 – 7451Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri60 – 13677UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: Ensembl
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. protein deubiquitination Source: Ensembl
  2. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.064.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 45 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 45
Ubiquitin thioesterase 45
Ubiquitin-specific-processing protease 45
Gene namesi
Name:Usp45
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:101850. Usp45.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 813813Ubiquitin carboxyl-terminal hydrolase 45PRO_0000280562Add
BLAST

Proteomic databases

MaxQBiQ8K387.
PaxDbiQ8K387.
PRIDEiQ8K387.

PTM databases

PhosphoSiteiQ8K387.

Expressioni

Gene expression databases

BgeeiQ8K387.
CleanExiMM_USP45.
ExpressionAtlasiQ8K387. baseline and differential.
GenevestigatoriQ8K387.

Interactioni

Protein-protein interaction databases

BioGridi218782. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8K387.
SMRiQ8K387. Positions 38-389, 602-810.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini191 – 812622USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri60 – 13677UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5560.
GeneTreeiENSGT00760000119203.
HOGENOMiHOG000154755.
HOVERGENiHBG062704.
InParanoidiQ8K387.
KOiK11844.
OMAiECEHISA.
OrthoDBiEOG7J9VNZ.
PhylomeDBiQ8K387.
TreeFamiTF326075.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8K387-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRVKDPSKDL PEKGKRNKRP LLPHDEDSSD DIAVGLTCQH VSYAVSVNHV
60 70 80 90 100
KKAVAESLWS VCSECLKERR FCDGQPVLPA DVWLCLKCGL QGCGKNSESQ
110 120 130 140 150
HSLRHFKSSG TESHCVVISL STWVIWCYEC NEKLSTHCNK KVLAQIVDFL
160 170 180 190 200
QKHAFKTQTG AFSRIIKLCE EKREAGEIKK GKKGCTVPSV KGITNLGNTC
210 220 230 240 250
FFNAVIQNLA QTYILFELMN EIKEDGTKFK ISLSSAPQLE PLVVELSSPG
260 270 280 290 300
PLTSALFLFL HSMKEAEKGP LSPKVLFNQL CQKAPRFKGF QQQDSQELLH
310 320 330 340 350
HLLDAVRTEE TKRIQASILK AFNNPTTKTA DDETRKKVKA YGKEGVKMNF
360 370 380 390 400
IDRIFIGELT STVMCEECAN ISTMKDPFID ISLPIIEERV SKPVLLGKMS
410 420 430 440 450
KCRSLQETDQ DHNKGTVTVG NAHQPRASRK HSSPNDKNQL SHDRKHLRKW
460 470 480 490 500
PSEEEKTVVT HPKNDNLEAS PPASTLSTEA SLNESLTDGS ERDASLESSV
510 520 530 540 550
DADSEASEPE IASKQPVLLR SRGDSCGHAE QHPHLPLASE LPQAKETHGG
560 570 580 590 600
EEEMAEAIAE LHLSGTVTGN RDFHREKQPL NVPNNLCFSE GKHTRLHSAQ
610 620 630 640 650
NAFQTLSQSY VTTSKECSVQ SCLYQFTSME LLMGNNKLLC EDCTEKRRKC
660 670 680 690 700
HKETSSAEKK AGGVYTNARK QLLISAVPAI LILHLKRFHQ AGLSLRKVNR
710 720 730 740 750
HVDFPLTLDL APFCAATCKN ISVGEKVLYG LYGIVEHSGS MRGGHYTAYV
760 770 780 790 800
KVRVPSRKLS ECITGRKTAA GLKEPDGELG GHWVHVSDTY VQVVPESRAL
810
SAQAYLLFYE RIL

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Length:813
Mass (Da):90,361
Last modified:October 1, 2002 - v1
Checksum:iED1DD9B9F007B178
GO
Isoform 2 (identifier: Q8K387-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     390-437: Missing.

Note: No experimental confirmation available.

Show »
Length:765
Mass (Da):85,110
Checksum:i08254CA4B7DEE012
GO

Sequence cautioni

The sequence BAC29631.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti595 – 5951R → G in BAC40129. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei390 – 43748Missing in isoform 2. 1 PublicationVSP_023794Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK036903 mRNA. Translation: BAC29631.1. Different initiation.
AK088073 mRNA. Translation: BAC40129.1.
AL772187 Genomic DNA. Translation: CAM22881.1.
BC027768 mRNA. Translation: AAH27768.1.
CCDSiCCDS38700.1. [Q8K387-1]
CCDS71351.1. [Q8K387-2]
RefSeqiNP_001277354.1. NM_001290425.1. [Q8K387-2]
NP_690038.1. NM_152825.2. [Q8K387-1]
XP_006538424.1. XM_006538361.1. [Q8K387-1]
UniGeneiMm.154306.

Genome annotation databases

EnsembliENSMUST00000040429; ENSMUSP00000048324; ENSMUSG00000040455. [Q8K387-2]
ENSMUST00000065111; ENSMUSP00000067109; ENSMUSG00000040455. [Q8K387-1]
ENSMUST00000108232; ENSMUSP00000103867; ENSMUSG00000040455. [Q8K387-1]
GeneIDi77593.
KEGGimmu:77593.
UCSCiuc008sda.1. mouse. [Q8K387-1]
uc008sdc.1. mouse. [Q8K387-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK036903 mRNA. Translation: BAC29631.1 . Different initiation.
AK088073 mRNA. Translation: BAC40129.1 .
AL772187 Genomic DNA. Translation: CAM22881.1 .
BC027768 mRNA. Translation: AAH27768.1 .
CCDSi CCDS38700.1. [Q8K387-1 ]
CCDS71351.1. [Q8K387-2 ]
RefSeqi NP_001277354.1. NM_001290425.1. [Q8K387-2 ]
NP_690038.1. NM_152825.2. [Q8K387-1 ]
XP_006538424.1. XM_006538361.1. [Q8K387-1 ]
UniGenei Mm.154306.

3D structure databases

ProteinModelPortali Q8K387.
SMRi Q8K387. Positions 38-389, 602-810.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 218782. 3 interactions.

Protein family/group databases

MEROPSi C19.064.

PTM databases

PhosphoSitei Q8K387.

Proteomic databases

MaxQBi Q8K387.
PaxDbi Q8K387.
PRIDEi Q8K387.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000040429 ; ENSMUSP00000048324 ; ENSMUSG00000040455 . [Q8K387-2 ]
ENSMUST00000065111 ; ENSMUSP00000067109 ; ENSMUSG00000040455 . [Q8K387-1 ]
ENSMUST00000108232 ; ENSMUSP00000103867 ; ENSMUSG00000040455 . [Q8K387-1 ]
GeneIDi 77593.
KEGGi mmu:77593.
UCSCi uc008sda.1. mouse. [Q8K387-1 ]
uc008sdc.1. mouse. [Q8K387-2 ]

Organism-specific databases

CTDi 85015.
MGIi MGI:101850. Usp45.

Phylogenomic databases

eggNOGi COG5560.
GeneTreei ENSGT00760000119203.
HOGENOMi HOG000154755.
HOVERGENi HBG062704.
InParanoidi Q8K387.
KOi K11844.
OMAi ECEHISA.
OrthoDBi EOG7J9VNZ.
PhylomeDBi Q8K387.
TreeFami TF326075.

Miscellaneous databases

NextBioi 347172.
PROi Q8K387.
SOURCEi Search...

Gene expression databases

Bgeei Q8K387.
CleanExi MM_USP45.
ExpressionAtlasi Q8K387. baseline and differential.
Genevestigatori Q8K387.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J and NOD.
    Tissue: Thymus and Vagina.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiUBP45_MOUSE
AccessioniPrimary (citable) accession number: Q8K387
Secondary accession number(s): A2AJT1, Q8BU19, Q8BZ19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3