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Protein

Ubiquitin carboxyl-terminal hydrolase 45

Gene

Usp45

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei200NucleophilePROSITE-ProRule annotation1
Active sitei745Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri60 – 136UBP-typePROSITE-ProRule annotationAdd BLAST77

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5696395. Formation of Incision Complex in GG-NER.

Protein family/group databases

MEROPSiC19.064.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 45 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 45
Ubiquitin thioesterase 45
Ubiquitin-specific-processing protease 45
Gene namesi
Name:Usp45
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:101850. Usp45.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002805621 – 813Ubiquitin carboxyl-terminal hydrolase 45Add BLAST813

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28PhosphoserineCombined sources1
Modified residuei29PhosphoserineCombined sources1
Modified residuei507PhosphoserineCombined sources1
Modified residuei525PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8K387.
PeptideAtlasiQ8K387.
PRIDEiQ8K387.

PTM databases

iPTMnetiQ8K387.
PhosphoSitePlusiQ8K387.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040455.
CleanExiMM_USP45.
ExpressionAtlasiQ8K387. baseline and differential.
GenevisibleiQ8K387. MM.

Interactioni

Protein-protein interaction databases

BioGridi218782. 5 interactors.
STRINGi10090.ENSMUSP00000067109.

Structurei

3D structure databases

ProteinModelPortaliQ8K387.
SMRiQ8K387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini191 – 812USPAdd BLAST622

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri60 – 136UBP-typePROSITE-ProRule annotationAdd BLAST77

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1873. Eukaryota.
COG5560. LUCA.
GeneTreeiENSGT00860000133682.
HOGENOMiHOG000154755.
HOVERGENiHBG062704.
InParanoidiQ8K387.
KOiK11844.
OMAiQHVSHAI.
OrthoDBiEOG091G01WT.
PhylomeDBiQ8K387.
TreeFamiTF326075.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
PfamiView protein in Pfam
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
SMARTiView protein in SMART
SM00290. ZnF_UBP. 1 hit.
PROSITEiView protein in PROSITE
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K387-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRVKDPSKDL PEKGKRNKRP LLPHDEDSSD DIAVGLTCQH VSYAVSVNHV
60 70 80 90 100
KKAVAESLWS VCSECLKERR FCDGQPVLPA DVWLCLKCGL QGCGKNSESQ
110 120 130 140 150
HSLRHFKSSG TESHCVVISL STWVIWCYEC NEKLSTHCNK KVLAQIVDFL
160 170 180 190 200
QKHAFKTQTG AFSRIIKLCE EKREAGEIKK GKKGCTVPSV KGITNLGNTC
210 220 230 240 250
FFNAVIQNLA QTYILFELMN EIKEDGTKFK ISLSSAPQLE PLVVELSSPG
260 270 280 290 300
PLTSALFLFL HSMKEAEKGP LSPKVLFNQL CQKAPRFKGF QQQDSQELLH
310 320 330 340 350
HLLDAVRTEE TKRIQASILK AFNNPTTKTA DDETRKKVKA YGKEGVKMNF
360 370 380 390 400
IDRIFIGELT STVMCEECAN ISTMKDPFID ISLPIIEERV SKPVLLGKMS
410 420 430 440 450
KCRSLQETDQ DHNKGTVTVG NAHQPRASRK HSSPNDKNQL SHDRKHLRKW
460 470 480 490 500
PSEEEKTVVT HPKNDNLEAS PPASTLSTEA SLNESLTDGS ERDASLESSV
510 520 530 540 550
DADSEASEPE IASKQPVLLR SRGDSCGHAE QHPHLPLASE LPQAKETHGG
560 570 580 590 600
EEEMAEAIAE LHLSGTVTGN RDFHREKQPL NVPNNLCFSE GKHTRLHSAQ
610 620 630 640 650
NAFQTLSQSY VTTSKECSVQ SCLYQFTSME LLMGNNKLLC EDCTEKRRKC
660 670 680 690 700
HKETSSAEKK AGGVYTNARK QLLISAVPAI LILHLKRFHQ AGLSLRKVNR
710 720 730 740 750
HVDFPLTLDL APFCAATCKN ISVGEKVLYG LYGIVEHSGS MRGGHYTAYV
760 770 780 790 800
KVRVPSRKLS ECITGRKTAA GLKEPDGELG GHWVHVSDTY VQVVPESRAL
810
SAQAYLLFYE RIL
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Length:813
Mass (Da):90,361
Last modified:October 1, 2002 - v1
Checksum:iED1DD9B9F007B178
GO
Isoform 2 (identifier: Q8K387-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     390-437: Missing.

Note: No experimental confirmation available.
Show »
Length:765
Mass (Da):85,110
Checksum:i08254CA4B7DEE012
GO

Sequence cautioni

The sequence BAC29631 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti595R → G in BAC40129 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023794390 – 437Missing in isoform 2. 1 PublicationAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036903 mRNA. Translation: BAC29631.1. Different initiation.
AK088073 mRNA. Translation: BAC40129.1.
AL772187 Genomic DNA. Translation: CAM22881.1.
BC027768 mRNA. Translation: AAH27768.1.
CCDSiCCDS38700.1. [Q8K387-1]
CCDS71351.1. [Q8K387-2]
RefSeqiNP_001277354.1. NM_001290425.1. [Q8K387-2]
NP_690038.1. NM_152825.2. [Q8K387-1]
XP_006538424.1. XM_006538361.3. [Q8K387-1]
XP_011248434.1. XM_011250132.1. [Q8K387-1]
UniGeneiMm.154306.

Genome annotation databases

EnsembliENSMUST00000040429; ENSMUSP00000048324; ENSMUSG00000040455. [Q8K387-2]
ENSMUST00000065111; ENSMUSP00000067109; ENSMUSG00000040455. [Q8K387-1]
ENSMUST00000108232; ENSMUSP00000103867; ENSMUSG00000040455. [Q8K387-1]
GeneIDi77593.
KEGGimmu:77593.
UCSCiuc008sda.1. mouse. [Q8K387-1]
uc008sdc.1. mouse. [Q8K387-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBP45_MOUSE
AccessioniPrimary (citable) accession number: Q8K387
Secondary accession number(s): A2AJT1, Q8BU19, Q8BZ19
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: October 1, 2002
Last modified: October 25, 2017
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families