Q8K377 (LRRT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine-rich repeat transmembrane neuronal protein 1

Gene names

Name:

Lrrtm1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Exhibits strong synaptogenic activity, restricted to excitatory presynaptic differentiation, acting at both pre- and postsynaptic level By similarity.
Subcellular location	Cell membrane; Single-pass type I membrane protein By similarity. Cell junction › synapse › postsynaptic cell membrane; Single-pass type I membrane protein By similarity. Note: Accumulates extensively in the endoplasmic reticulum of transfected nonneuronal cells (Ref.6). Ref.5 Ref.6
Tissue specificity	Expressed predominantly in the nervous system by postmitotic neurons, but also in some non-neuronal tissues. In adult brain expression is most prominent in the forebraain, particularly in the thalamus and in the cortical areas including hippocampus, piriform and posterior cingulate. Ref.1 Ref.6
Developmental stage	Initially detected at 9 dpc with expression present in the overlying ectoderm of the limb bud in the presumptive apical ectodermal ridge. Expression is also seen in the dorsal otic vesicle in the presumptive endolymphatic appendage. Neural expression is present in the forebrain and midbrain with a sharp boundary across the central midbrain. Expression is also seen in the hindbrain. A stripe of expression can be detected in the neural tube. At 10 and 11 dpc expression is restricted to the apical ectodermal ridge. Expression persists in the endolymphatic diverticular appendage of the otic vesicle the forebrain/midbrain and the ventricular layer of the central neural tube through these stages. Ref.4
Disruption phenotype	Mutant animals survive in the expected Mendelian ratios, are fertile and display no overt phenotype. Brain morphology appears grossly normal, except for rare cases of anomalous ventroculomegaly. Increase in the presynaptic area occupied by SLC17A7/VGLUT1 in some strata of the hippocampal neuropil of the CA1 region. Ref.7
Sequence similarities	Belongs to the LRRTM family. Contains 10 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Leucine-rich repeat Repeat Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	behavior Inferred from mutant phenotype PubMed 21818371. Source: MGI protein localization to synapse Inferred from mutant phenotype Ref.7. Source: MGI synapse organization Inferred from mutant phenotype Ref.7PubMed 21818371. Source: MGI
Cellular_component	axon Inferred from electronic annotation. Source: Compara cell junction Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: Compara excitatory synapse Inferred from electronic annotation. Source: Compara growth cone Inferred from electronic annotation. Source: Compara integral to membrane Inferred from sequence or structural similarity Ref.1. Source: MGI plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Potential
	Chain	35 – 522	488	Leucine-rich repeat transmembrane neuronal protein 1		PRO_0000018351
Regions
	Topological domain	35 – 427	393	Extracellular Potential
	Transmembrane	428 – 448	21	Helical; Potential
	Topological domain	449 – 522	74	Cytoplasmic Potential
	Domain	35 – 63	29	LRRNT
	Repeat	64 – 87	24	LRR 1
	Repeat	89 – 111	23	LRR 2
	Repeat	112 – 135	24	LRR 3
	Repeat	136 – 159	24	LRR 4
	Repeat	161 – 183	23	LRR 5
	Repeat	184 – 207	24	LRR 6
	Repeat	209 – 231	23	LRR 7
	Repeat	233 – 255	23	LRR 8
	Repeat	256 – 278	23	LRR 9
	Repeat	280 – 302	23	LRR 10
	Domain	314 – 365	52	LRRCT
Amino acid modifications
	Glycosylation	56	1	N-linked (GlcNAc...) Potential
	Glycosylation	63	1	N-linked (GlcNAc...) Potential
	Glycosylation	130	1	N-linked (GlcNAc...) Potential
	Glycosylation	381	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8K377 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 6EBBA18034539DCC	FASTA	522	58,718
	10 20 30 40 50 60 MDFLLLGLCL HWLLRRPSGV VLCLLGACFQ MLPAAPSGCP GQCRCEGRLL YCEALNLTEA 70 80 90 100 110 120 PHNLSGLLGL SLRYNSLSEL RAGQFTGLMQ LTWLYLDHNH ICSVQGDAFQ KLRRVKELTL 130 140 150 160 170 180 SSNQITELAN TTFRPMPNLR SVDLSYNKLQ ALAPDLFHGL RKLTTLHMRA NAIQFVPVRI 190 200 210 220 230 240 FQDCRSLKFL DIGYNQLKSL ARNSFAGLFK LTELHLEHND LIKVNFAHFP RLISLHSLCL 250 260 270 280 290 300 RRNKVAIVVS SLDWVWNLEK MDLSGNEIEY MEPHVFETVP YLQTLQLDSN RLTYIEPRIL 310 320 330 340 350 360 NSWKSLTSIT LAGNLWDCGR NVCALASWLS NFQGRYDANL QCASPEYAQG EDVLDAVYAF 370 380 390 400 410 420 HLCEDGAEPT SGHLLSVAVT NRSDLTPPES SATTLVDGGE GHDGTFEPIT VALPGGEHAE 430 440 450 460 470 480 NAVQIHKVVT GTMALIFSFL IVVLVLYVSW KCFPASLRQL RQCFVTQRRK QKQKQTMHQM 490 500 510 520 AAMSAQEYYV DYKPNHIEGA LVIINEYGSC TCHQQPAREC EV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel gene family encoding leucine-rich repeat transmembrane proteins differentially expressed in the nervous system." Lauren J., Airaksinen M.S., Saarma M., Timmusk T.T. Genomics 81:411-421(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: C57BL/6.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Diencephalon and Eye.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[4]	"Developmentally regulated expression of the LRRTM gene family during mid-gestation mouse embryogenesis." Haines B.P., Rigby P.W.J. Gene Expr. Patterns 7:23-29(2007) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[5]	"LRRTM1 protein is located in the endoplasmic reticulum (ER) in mammalian cells." Francks C., Maegawa S., Lauren J., Abrahams B.S., Velayos-Baeza A., Medland S.E., Colella S., Groszer M., McAuley E.Z., Caffrey T.M., Timmusk T., Pruunsild P., Koppel I., Lind P.A., Matsumoto-Itaba N., Nicod J., Xiong L., Joober R. , Enard W., Krinsky B., Nanba E., Richardson A.J., Riley B.P., Martin N.G., Strittmatter S.M., Moeller H.J., Rujescu D., St Clair D., Muglia P., Roos J.L., Fisher S.E., Wade-Martins R., Rouleau G.A., Stein J.F., Karayiorgou M., Geschwind D.H., Ragoussis J., Kendler K.S., Airaksinen M.S., Oshimura M., DeLisi L.E., Monaco A.P. Mol. Psychiatry 12:1057-1057(2007) Cited for: SUBCELLULAR LOCATION.
[6]	"LRRTM1 on chromosome 2p12 is a maternally suppressed gene that is associated paternally with handedness and schizophrenia." Francks C., Maegawa S., Lauren J., Abrahams B.S., Velayos-Baeza A., Medland S.E., Colella S., Groszer M., McAuley E.Z., Caffrey T.M., Timmusk T., Pruunsild P., Koppel I., Lind P.A., Matsumoto-Itaba N., Nicod J., Xiong L., Joober R. , Enard W., Krinsky B., Nanba E., Richardson A.J., Riley B.P., Martin N.G., Strittmatter S.M., Moeller H.J., Rujescu D., St Clair D., Muglia P., Roos J.L., Fisher S.E., Wade-Martins R., Rouleau G.A., Stein J.F., Karayiorgou M., Geschwind D.H., Ragoussis J., Kendler K.S., Airaksinen M.S., Oshimura M., DeLisi L.E., Monaco A.P. Mol. Psychiatry 12:1129-1139(2007) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[7]	"An unbiased expression screen for synaptogenic proteins identifies the LRRTM protein family as synaptic organizers." Linhoff M.W., Lauren J., Cassidy R.M., Dobie F.A., Takahashi H., Nygaard H.B., Airaksinen M.S., Strittmatter S.M., Craig A.M. Neuron 61:734-749(2009) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

The hands to say it - Issue 91 of February 2008

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY182025 mRNA. Translation: AAO67546.1. AK053345 mRNA. Translation: BAC35354.1. AK053364 mRNA. Translation: BAC35363.1. AK136857 mRNA. Translation: BAE23148.1. BC027803 mRNA. Translation: AAH27803.1.
IPI	IPI00170022.
RefSeq	NP_083156.2. NM_028880.3.
UniGene	Mm.292568.
3D structure databases
ProteinModelPortal	Q8K377.
SMR	Q8K377. Positions 39-399.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8K377. 1 interaction.
PTM databases
PhosphoSite	Q8K377.
Proteomic databases
PaxDb	Q8K377.
PRIDE	Q8K377.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000020400; ENSMUSP00000020400; ENSMUSG00000060780. ENSMUST00000159616; ENSMUSP00000125207; ENSMUSG00000060780. ENSMUST00000161677; ENSMUSP00000124373; ENSMUSG00000060780.
GeneID	74342.
KEGG	mmu:74342.
UCSC	uc009cjt.1. mouse.
Organism-specific databases
CTD	347730.
MGI	MGI:2389173. Lrrtm1.
Phylogenomic databases
eggNOG	COG4886.
GeneTree	ENSGT00700000104056.
HOGENOM	HOG000290681.
HOVERGEN	HBG052362.
InParanoid	Q8K377.
KO	K16665.
OMA	EPDLFHG.
OrthoDB	EOG4FN4HG.
Gene expression databases
ArrayExpress	Q8K377.
Bgee	Q8K377.
CleanEx	MM_LRRTM1.
Genevestigator	Q8K377.
GermOnline	ENSMUSG00000060780. Mus musculus.
Family and domain databases
InterPro	IPR001611. Leu-rich_rpt. IPR003591. Leu-rich_rpt_typical-subtyp. IPR000372. LRR-contain_N. [Graphical view]
SMART	SM00369. LRR_TYP. 3 hits. SM00013. LRRNT. 1 hit. [Graphical view]
PROSITE	PS51450. LRR. 9 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	340493.
SOURCE	Search...

Entry information

Entry name

LRRT1_MOUSE

Accession

Primary (citable) accession number: Q8K377
Secondary accession number(s): Q3UVX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program