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Protein

DNA/RNA-binding protein KIN17

Gene

Kin

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo. Binds via its C-terminal domain to RNA in vitro.By similarity4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 5023C2H2-typeSequence analysisAdd
BLAST

GO - Molecular functioni

  • double-stranded DNA binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • RNA binding Source: UniProtKB

GO - Biological processi

  • cellular response to DNA damage stimulus Source: UniProtKB
  • DNA recombination Source: UniProtKB-KW
  • DNA repair Source: UniProtKB-KW
  • DNA replication Source: UniProtKB
  • mRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, DNA replication, mRNA processing, Stress response

Keywords - Ligandi

DNA-binding, Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA/RNA-binding protein KIN17
Alternative name(s):
Binding to curved DNA
KIN, antigenic determinant of recA protein
Gene namesi
Name:KinImported
Synonyms:Btcd, Kin17
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:96676. Kin.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: MGI
  • nuclear matrix Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 391391DNA/RNA-binding protein KIN17PRO_0000289135Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei135 – 1351N6,N6,N6-trimethyllysine; by METTL22By similarity

Keywords - PTMi

Methylation

Proteomic databases

EPDiQ8K339.
MaxQBiQ8K339.
PaxDbiQ8K339.
PeptideAtlasiQ8K339.
PRIDEiQ8K339.

PTM databases

iPTMnetiQ8K339.
PhosphoSiteiQ8K339.

Expressioni

Tissue specificityi

Highly expressed in transformed mouse AtT20 neuroendocrine cells. Expressed at a lower level in testis, kidney, skeletal muscle, liver, lung, spleen, brain and heart and kidney. In testis, expressed at much higher levels in proliferating cells than in differentiating cells. Not detected in embryo.3 Publications

Developmental stagei

In testis, expression almost doubled from day 5 to days 17-22 postpartum (dpp) and then decreased by 28 dpp to reach a stable level in adult testis.1 Publication

Gene expression databases

BgeeiQ8K339.
CleanExiMM_KIN.
GenevisibleiQ8K339. MM.

Interactioni

Subunit structurei

Associated with DNA polymerase alpha, RFC1 and cyclin A, in multiprotein DNA replication complexes. Also associates with replication origins at the G1/S phase boundary and throughout the S phase in vivo (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8K339. 2 interactions.
MINTiMINT-4117607.
STRINGi10090.ENSMUSP00000043614.

Structurei

3D structure databases

ProteinModelPortaliQ8K339.
SMRiQ8K339. Positions 51-160, 272-391.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 160110Winged helix-turn-helix (wHTH)By similarityAdd
BLAST
Regioni282 – 33251C-terminal subdomain ABy similarityAdd
BLAST
Regioni338 – 38952C-terminal subdomain BBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili147 – 18034Sequence analysisAdd
BLAST
Coiled coili252 – 27524Sequence analysisAdd
BLAST

Domaini

The C-terminal domain (268-393) is organized into 2 subdomains that bear structural similarities to SH3-like domains. Both subdomains adopt a similar 5-stranded beta-barrel-like fold and are connected to each other by a short linker of 5 residues. The 5 beta-sheets are packed at approximately right angles against each other. A highly conserved groove formed at the interface between the 2 subdomains, comprised of Lys residues 302 and 391 and other positively charged residues, may possibly be the site of RNA-binding (By similarity).By similarity

Sequence similaritiesi

Belongs to the KIN17 family.Curated
Contains 1 C2H2-type zinc finger.Sequence analysis

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 5023C2H2-typeSequence analysisAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG2837. Eukaryota.
ENOG410XRJ7. LUCA.
GeneTreeiENSGT00390000005903.
HOGENOMiHOG000178663.
HOVERGENiHBG053068.
InParanoidiQ8K339.
KOiK13102.
OMAiESINEKA.
OrthoDBiEOG700887.
PhylomeDBiQ8K339.
TreeFamiTF314393.

Family and domain databases

InterProiIPR019447. DNA/RNA-bd_Kin17_cons_domain.
[Graphical view]
PfamiPF10357. Kin17_mid. 1 hit.
[Graphical view]
SMARTiSM01253. Kin17_mid. 1 hit.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K339-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKSDFLSPK AIANRIKSKG LQKLRWYCQM CQKQCRDENG FKCHCMSESH
60 70 80 90 100
QRQLLLASEN PQQFMDYFSE EFRNDFLELL RRRFGTKRVH NNIVYNEYIS
110 120 130 140 150
HREHIHMNAT QWETLTDFTK WLGREGLCKV DETPKGWYIQ YIDRDPETIR
160 170 180 190 200
RQLELEKKKK QDLDDEEKTA KFIEEQVRRG LEGKEQETPV FTELSRENEE
210 220 230 240 250
EKVTFNLNKG AGGSAGATTS KSSSLGPSAL KLLGSAASGK RKESSQSSAQ
260 270 280 290 300
PAKKKKSALD EIMELEEEKK RTARTDAWLQ PGIVVKIITK KLGEKYHKKK
310 320 330 340 350
GVVKEVIDRY TAVVKMTDSG DRLKLDQTHL ETVIPAPGKR VLVLNGGYRG
360 370 380 390
NEGTLESINE KAFSATIVIE TGPLKGRRVE GIQYEDISKL A
Length:391
Mass (Da):44,722
Last modified:October 1, 2002 - v1
Checksum:i955BC6A4FF4D3B6E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58472 mRNA. No translation available.
AK009429 mRNA. Translation: BAB26281.3.
AK167740 mRNA. Translation: BAE39778.1.
AL772367 Genomic DNA. Translation: CAM13915.1.
BC028860 mRNA. Translation: AAH28860.1.
CCDSiCCDS15676.1.
PIRiS18666.
RefSeqiNP_079556.1. NM_025280.2.
UniGeneiMm.35498.

Genome annotation databases

EnsembliENSMUST00000042512; ENSMUSP00000043614; ENSMUSG00000037262.
GeneIDi16588.
KEGGimmu:16588.
UCSCiuc008ihp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58472 mRNA. No translation available.
AK009429 mRNA. Translation: BAB26281.3.
AK167740 mRNA. Translation: BAE39778.1.
AL772367 Genomic DNA. Translation: CAM13915.1.
BC028860 mRNA. Translation: AAH28860.1.
CCDSiCCDS15676.1.
PIRiS18666.
RefSeqiNP_079556.1. NM_025280.2.
UniGeneiMm.35498.

3D structure databases

ProteinModelPortaliQ8K339.
SMRiQ8K339. Positions 51-160, 272-391.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8K339. 2 interactions.
MINTiMINT-4117607.
STRINGi10090.ENSMUSP00000043614.

PTM databases

iPTMnetiQ8K339.
PhosphoSiteiQ8K339.

Proteomic databases

EPDiQ8K339.
MaxQBiQ8K339.
PaxDbiQ8K339.
PeptideAtlasiQ8K339.
PRIDEiQ8K339.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042512; ENSMUSP00000043614; ENSMUSG00000037262.
GeneIDi16588.
KEGGimmu:16588.
UCSCiuc008ihp.1. mouse.

Organism-specific databases

CTDi22944.
MGIiMGI:96676. Kin.

Phylogenomic databases

eggNOGiKOG2837. Eukaryota.
ENOG410XRJ7. LUCA.
GeneTreeiENSGT00390000005903.
HOGENOMiHOG000178663.
HOVERGENiHBG053068.
InParanoidiQ8K339.
KOiK13102.
OMAiESINEKA.
OrthoDBiEOG700887.
PhylomeDBiQ8K339.
TreeFamiTF314393.

Miscellaneous databases

PROiQ8K339.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K339.
CleanExiMM_KIN.
GenevisibleiQ8K339. MM.

Family and domain databases

InterProiIPR019447. DNA/RNA-bd_Kin17_cons_domain.
[Graphical view]
PfamiPF10357. Kin17_mid. 1 hit.
[Graphical view]
SMARTiSM01253. Kin17_mid. 1 hit.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and expression of the cDNA of KIN17, a zinc-finger gene located on mouse chromosome 2, encoding a new DNA-binding protein."
    Angulo J.F., Rouer E., Mazin A., Mattei M.-G., Tissier A., Horellou P., Benarous R., Devoret R.
    Nucleic Acids Res. 19:5117-5123(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: Embryonic brain1 Publication.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6JImported and DBA/2Imported.
    Tissue: TongueImported.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/NImported.
    Tissue: Mammary tumorImported.
  5. "Identification of a mouse cDNA fragment whose expressed polypeptide reacts with anti-recA antibodies."
    Angulo J.F., Rouer E., Benarous R., Devoret R.
    Biochimie 73:251-256(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 74-273.
  6. "Btcd, a mouse protein that binds to curved DNA, can substitute in Escherichia coli for H-NS, a bacterial nucleoid protein."
    Timchenko T., Bailone A., Devoret R.
    EMBO J. 15:3986-3992(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Molecular cloning and characterization of the mouse Kin17 gene coding for a Zn-finger protein that preferentially recognizes bent DNA."
    Tissier A., Kannouche P., Mauffrey P., Allemand I., Frelat G., Devoret R., Angulo J.F.
    Genomics 38:238-242(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  8. "KIN17 encodes an RNA-binding protein and is expressed during mouse spermatogenesis."
    Pinon-Lataillade G., Masson C., Bernardino-Sgherri J., Henriot V., Mauffrey P., Frobert Y., Araneda S., Angulo J.F.
    J. Cell Sci. 117:3691-3702(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiKIN17_MOUSE
AccessioniPrimary (citable) accession number: Q8K339
Secondary accession number(s): Q9CV58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 1, 2002
Last modified: July 6, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Recognized by antibodies directed against the RecA protein.2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.