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Q8K330

- SSH3_MOUSE

UniProt

Q8K330 - SSH3_MOUSE

Protein

Protein phosphatase Slingshot homolog 3

Gene

Ssh3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly.1 Publication

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei410 – 4101Phosphocysteine intermediateCurated

    GO - Molecular functioni

    1. actin binding Source: RefGenome
    2. DNA binding Source: InterPro
    3. protein tyrosine/serine/threonine phosphatase activity Source: RefGenome
    4. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein dephosphorylation Source: RefGenome
    2. regulation of actin polymerization or depolymerization Source: RefGenome
    3. regulation of axonogenesis Source: RefGenome
    4. regulation of lamellipodium assembly Source: RefGenome

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein phosphatase Slingshot homolog 3 (EC:3.1.3.16, EC:3.1.3.48)
    Alternative name(s):
    SSH-like protein 3
    Short name:
    SSH-3L
    Short name:
    mSSH-3L
    Gene namesi
    Name:Ssh3
    Synonyms:Ssh3l
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:2683546. Ssh3.

    Subcellular locationi

    Cytoplasmcytoskeleton 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome
    2. cytoskeleton Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi410 – 4101C → S: Abrogates phosphatase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 649648Protein phosphatase Slingshot homolog 3PRO_0000094846Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei88 – 881PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ8K330.
    PRIDEiQ8K330.

    PTM databases

    PhosphoSiteiQ8K330.

    Expressioni

    Tissue specificityi

    Expressed in brain, small intestine and testis. Also expressed at lower levels in heart, kidney, liver, spleen and thymus.1 Publication

    Developmental stagei

    Expressed in the nervous system and epithelial tissues of the trachea at E14.5.1 Publication

    Gene expression databases

    BgeeiQ8K330.
    CleanExiMM_SSH3.
    GenevestigatoriQ8K330.

    Interactioni

    Subunit structurei

    Does not bind to, or colocalize with, filamentous actin.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K330.
    SMRiQ8K330. Positions 327-466.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini325 – 465141Tyrosine-protein phosphataseAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2453.
    GeneTreeiENSGT00750000117282.
    HOGENOMiHOG000154428.
    HOVERGENiHBG089321.
    KOiK05766.
    OMAiPHWKETH.
    OrthoDBiEOG7B8S33.
    PhylomeDBiQ8K330.
    TreeFamiTF319444.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    3.90.190.10. 1 hit.
    InterProiIPR014876. DEK_C.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR009057. Homeodomain-like.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF08766. DEK_C. 1 hit.
    PF00782. DSPc. 1 hit.
    [Graphical view]
    SMARTiSM00195. DSPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8K330-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MALVTVSRSP PASGHSTPVG PTQDRVVRRR GRLQRRQSFA VLRGAVLGLQ    50
    DGGDSNVASE ADSEPMEEPS GEEQPTEDQT DKGQGLQSPW KQVQKRHLHL 100
    MVELLRPQDD IRLAAQLEAA RPPRLRYLLV VSTGEELSEE AILLGVDFPD 150
    SSSHSCTLGL VLPLWSDTQV YLDGDGGFSV TSGGQSRIFK PVSIQTMWAT 200
    LQVLHQACEV ALGSGLVPGG SALAWATYYQ EKLNSDQGCL NEWMAMSDLE 250
    SFRPPNAEPG QASEQEKMEQ AILAELWQVL DTSDLDSVTS KEIRQALELR 300
    LGCPLQQYRD FIDNQMLLLM AQQDRASRIF PHLYLGSEWN AANLEELQKN 350
    RVSHILNMAR EIDNFFPERF TYYNVRVWDE ESAQLLPHWK ETHRFIEDAR 400
    AQGTRVLVHC KMGVSRSAAT VLAYAMKQYG WDLEQALIHV QELRPIVRPN 450
    HGFLRQLRTY QGILTASRQS HVWEQKVGVV SPEEPLAPEV STPLPPLPPE 500
    PGGSGEVMVM GLEGSQETPK EELGLRPRIN LRGVMRSISL LEPSESESTP 550
    EAGGLPEVFS SDEEPLHPFS QLSRAKGGQR VRKGPWPALK SRQSVVALHS 600
    AALVASRTRA FQEQGQGQEQ SEPGMSSTPR LRKVMRQASV DDSREEDKA 649
    Length:649
    Mass (Da):72,227
    Last modified:October 1, 2002 - v1
    Checksum:iDF8628B5E007E6F0
    GO
    Isoform 2 (identifier: Q8K330-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         261-261: Q → QITTR

    Note: No experimental confirmation available.

    Show »
    Length:653
    Mass (Da):72,699
    Checksum:iA9674B3180E0648A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti74 – 741Q → R in BAE29141. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei261 – 2611Q → QITTR in isoform 2. 1 PublicationVSP_016337

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB099289 mRNA. Translation: BAC97812.1.
    BC028922 mRNA. Translation: AAH28922.1.
    AK149880 mRNA. Translation: BAE29141.1.
    CCDSiCCDS29425.1. [Q8K330-1]
    RefSeqiNP_932781.1. NM_198113.2. [Q8K330-1]
    XP_006531811.1. XM_006531748.1. [Q8K330-2]
    UniGeneiMm.248388.

    Genome annotation databases

    EnsembliENSMUST00000037992; ENSMUSP00000047718; ENSMUSG00000034616. [Q8K330-1]
    ENSMUST00000113852; ENSMUSP00000109483; ENSMUSG00000034616. [Q8K330-2]
    GeneIDi245857.
    KEGGimmu:245857.
    UCSCiuc008fzo.2. mouse. [Q8K330-1]
    uc012bgi.2. mouse. [Q8K330-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB099289 mRNA. Translation: BAC97812.1 .
    BC028922 mRNA. Translation: AAH28922.1 .
    AK149880 mRNA. Translation: BAE29141.1 .
    CCDSi CCDS29425.1. [Q8K330-1 ]
    RefSeqi NP_932781.1. NM_198113.2. [Q8K330-1 ]
    XP_006531811.1. XM_006531748.1. [Q8K330-2 ]
    UniGenei Mm.248388.

    3D structure databases

    ProteinModelPortali Q8K330.
    SMRi Q8K330. Positions 327-466.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8K330.

    Proteomic databases

    PaxDbi Q8K330.
    PRIDEi Q8K330.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000037992 ; ENSMUSP00000047718 ; ENSMUSG00000034616 . [Q8K330-1 ]
    ENSMUST00000113852 ; ENSMUSP00000109483 ; ENSMUSG00000034616 . [Q8K330-2 ]
    GeneIDi 245857.
    KEGGi mmu:245857.
    UCSCi uc008fzo.2. mouse. [Q8K330-1 ]
    uc012bgi.2. mouse. [Q8K330-2 ]

    Organism-specific databases

    CTDi 54961.
    MGIi MGI:2683546. Ssh3.

    Phylogenomic databases

    eggNOGi COG2453.
    GeneTreei ENSGT00750000117282.
    HOGENOMi HOG000154428.
    HOVERGENi HBG089321.
    KOi K05766.
    OMAi PHWKETH.
    OrthoDBi EOG7B8S33.
    PhylomeDBi Q8K330.
    TreeFami TF319444.

    Miscellaneous databases

    NextBioi 386960.
    PROi Q8K330.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8K330.
    CleanExi MM_SSH3.
    Genevestigatori Q8K330.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    3.90.190.10. 1 hit.
    InterProi IPR014876. DEK_C.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR009057. Homeodomain-like.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view ]
    PANTHERi PTHR10159. PTHR10159. 1 hit.
    Pfami PF08766. DEK_C. 1 hit.
    PF00782. DSPc. 1 hit.
    [Graphical view ]
    SMARTi SM00195. DSPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Differential activities, subcellular distribution and tissue expression patterns of three members of Slingshot family phosphatases that dephosphorylate cofilin."
      Ohta Y., Kousaka K., Nagata-Ohashi K., Ohashi K., Muramoto A., Shima Y., Niwa R., Uemura T., Mizuno K.
      Genes Cells 8:811-824(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF CYS-410.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: Czech II.
      Tissue: Mammary tumor.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-582 (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Bone marrow.
    4. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiSSH3_MOUSE
    AccessioniPrimary (citable) accession number: Q8K330
    Secondary accession number(s): Q3UDX0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Tyrosine phosphatase activity has not been demonstrated for this protein to date.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3