Reviewed, UniProtKB/Swiss-Prot Q8K2Z8 (UB2Q2_MOUSE)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 Q2
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase Q2
    Ubiquitin carrier protein Q2

Gene names

Name:

Ube2q2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8K2Z8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8K2Z8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Ubiquitin-conjugating enzyme E2 Q2		PRO_0000223880
Regions
	Compositional bias	41 – 56	16	Pro-rich
	Compositional bias	138 – 181	44	Glu-rich
Sites
	Active site	307	1	Glycyl thioester intermediate By similarity
Amino acid modifications
	Modified residue	372	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	1 – 123	123	Missing in isoform 2.		VSP_017300
	Natural variant	235	1	I → T in strain: FVB/N. Ref.2
Experimental info
	Sequence conflict	246	1	L → Q in BAC38813. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 21, 2006. Version 2. Checksum: 4A81D0AC4BD302E3	FASTA	378	42,935
	10 20 30 40 50 60 MSVSGLKAEL KFLASIFDKN HERFRIVSWK LDELHCQFLV PPPPPPPGSS LSPPPPLTLH 70 80 90 100 110 120 CNITESYPSS SPIWFVDSDD PNLTSVLERL EDTKNNSSLR QQLKWLICDL CRLYNLPKHL 130 140 150 160 170 180 DVEMLDQPLP TGQNGTTEEV TSEEEEEEEM AEDIEDLDHY EMKEEEPING KKSEDEGIEK 190 200 210 220 230 240 ENLAILEKIR KTQRQDHLNG AVSGSVQASD RLMKELRDVY RSQSYKAGIY SVELINDSLY 250 260 270 280 290 300 DWHVKLHKVD SDSPLHSDLQ ILKEKEGIEY ILLNFSFKDN FPFDPPFVRV VLPVLSGGYV 310 320 330 340 350 360 LGGGALCMEL LTKQGWSSAY SIESVIMQIN ATLVKGKARV QFGANKNQYN LARAQQSYNS 370 IVQIHEKNGW YTPPKEDG
	Isoform 2. Checksum: 2F5077B3FAC2D6DF	FASTA	255	28,916
	10 20 30 40 50 60 MLDQPLPTGQ NGTTEEVTSE EEEEEEMAED IEDLDHYEMK EEEPINGKKS EDEGIEKENL 70 80 90 100 110 120 AILEKIRKTQ RQDHLNGAVS GSVQASDRLM KELRDVYRSQ SYKAGIYSVE LINDSLYDWH 130 140 150 160 170 180 VKLHKVDSDS PLHSDLQILK EKEGIEYILL NFSFKDNFPF DPPFVRVVLP VLSGGYVLGG 190 200 210 220 230 240 GALCMELLTK QGWSSAYSIE SVIMQINATL VKGKARVQFG ANKNQYNLAR AQQSYNSIVQ 250 IHEKNGWYTP PKEDG

References

[1]

"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Bone marrow, Fetal head, Hippocampus and Thymus.

[2]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-235. Strain: FVB/N. Tissue: Mammary tumor.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK083216 mRNA. Translation: BAC38813.1. AK076148 mRNA. Translation: BAC36218.1. AK042515 mRNA. Translation: BAE20633.1. AK150776 mRNA. Translation: BAE29841.1. BC029111 mRNA. Translation: AAH29111.1.
IPI	IPI00345026. IPI00719933.
RefSeq	NP_850931.1.
UniGene	Mm.207894
3D structure databases
SMR	Q8K2Z8. Positions 204-365.
ModBase	Search...
PTM databases
PhosphoSite	Q8K2Z8.
Genome annotation databases
Ensembl	ENSMUSG00000032307. Mus musculus. [Contig view]
GeneID	109161.
KEGG	mmu:109161.
Organism-specific databases
MGI	MGI:2388672. Ube2q2.
Phylogenomic databases
HOGENOM	Q8K2Z8.
HOVERGEN	Q8K2Z8.
OMA	Q8K2Z8. YKAGIYS.
Enzyme and pathway databases
BRENDA	6.3.2.19. 244.
Gene expression databases
ArrayExpress	Q8K2Z8.
Bgee	Q8K2Z8.
CleanEx	MM_UBE2Q2.
GermOnline	ENSMUSG00000032307. Mus musculus.
Family and domain databases
InterPro	IPR006575. RWD. IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF05773. RWD. 1 hit. PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00591. RWD. 1 hit. SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	361712.
SOURCE	Search...

Entry information

Entry name

UB2Q2_MOUSE

Accession

Primary (citable) accession number: Q8K2Z8
Secondary accession number(s): Q3UBX3 , Q3V3A5, Q8BUN2, Q8BVX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	February 21, 2006
Last modified:	June 16, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)