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Protein

Ubiquitin-conjugating enzyme E2 Q2

Gene

Ube2q2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination (By similarity).By similarity

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei307 – 3071Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. ubiquitin conjugating enzyme activity Source: MGI
  4. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. protein K48-linked ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 Q2 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein Q2
Ubiquitin-protein ligase Q2
Gene namesi
Name:Ube2q2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2388672. Ube2q2.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 378378Ubiquitin-conjugating enzyme E2 Q2PRO_0000223880Add
BLAST

Proteomic databases

MaxQBiQ8K2Z8.
PaxDbiQ8K2Z8.
PRIDEiQ8K2Z8.

PTM databases

PhosphoSiteiQ8K2Z8.

Expressioni

Gene expression databases

BgeeiQ8K2Z8.
CleanExiMM_UBE2Q2.
ExpressionAtlasiQ8K2Z8. baseline and differential.
GenevestigatoriQ8K2Z8.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000059798.

Structurei

3D structure databases

ProteinModelPortaliQ8K2Z8.
SMRiQ8K2Z8. Positions 204-365.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi41 – 5616Pro-richAdd
BLAST
Compositional biasi138 – 18144Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG320521.
GeneTreeiENSGT00390000018573.
HOGENOMiHOG000007757.
HOVERGENiHBG061368.
InParanoidiQ8K2Z8.
KOiK10582.
OMAiSPLRCEQ.
OrthoDBiEOG76DTSS.
PhylomeDBiQ8K2Z8.
TreeFamiTF313338.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR006575. RWD-domain.
IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF05773. RWD. 1 hit.
PF00179. UQ_con. 1 hit.
[Graphical view]
SMARTiSM00591. RWD. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 2 hits.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K2Z8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVSGLKAEL KFLASIFDKN HERFRIVSWK LDELHCQFLV PPPPPPPGSS
60 70 80 90 100
LSPPPPLTLH CNITESYPSS SPIWFVDSDD PNLTSVLERL EDTKNNSSLR
110 120 130 140 150
QQLKWLICDL CRLYNLPKHL DVEMLDQPLP TGQNGTTEEV TSEEEEEEEM
160 170 180 190 200
AEDIEDLDHY EMKEEEPING KKSEDEGIEK ENLAILEKIR KTQRQDHLNG
210 220 230 240 250
AVSGSVQASD RLMKELRDVY RSQSYKAGIY SVELINDSLY DWHVKLHKVD
260 270 280 290 300
SDSPLHSDLQ ILKEKEGIEY ILLNFSFKDN FPFDPPFVRV VLPVLSGGYV
310 320 330 340 350
LGGGALCMEL LTKQGWSSAY SIESVIMQIN ATLVKGKARV QFGANKNQYN
360 370
LARAQQSYNS IVQIHEKNGW YTPPKEDG
Length:378
Mass (Da):42,935
Last modified:February 20, 2006 - v2
Checksum:i4A81D0AC4BD302E3
GO
Isoform 2 (identifier: Q8K2Z8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.

Note: No experimental confirmation available.

Show »
Length:255
Mass (Da):28,916
Checksum:i2F5077B3FAC2D6DF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti246 – 2461L → Q in BAC38813 (PubMed:16141072).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti235 – 2351I → T in strain: FVB/N. 1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 123123Missing in isoform 2. 1 PublicationVSP_017300Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK083216 mRNA. Translation: BAC38813.1.
AK076148 mRNA. Translation: BAC36218.1.
AK042515 mRNA. Translation: BAE20633.1.
AK150776 mRNA. Translation: BAE29841.1.
BC029111 mRNA. Translation: AAH29111.1.
CCDSiCCDS23201.1. [Q8K2Z8-1]
RefSeqiNP_850931.2. NM_180600.3. [Q8K2Z8-1]
XP_006510831.1. XM_006510768.2. [Q8K2Z8-2]
UniGeneiMm.207894.

Genome annotation databases

EnsembliENSMUST00000059555; ENSMUSP00000059798; ENSMUSG00000032307. [Q8K2Z8-1]
ENSMUST00000122441; ENSMUSP00000112745; ENSMUSG00000032307. [Q8K2Z8-2]
GeneIDi109161.
KEGGimmu:109161.
UCSCiuc009psb.2. mouse. [Q8K2Z8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK083216 mRNA. Translation: BAC38813.1.
AK076148 mRNA. Translation: BAC36218.1.
AK042515 mRNA. Translation: BAE20633.1.
AK150776 mRNA. Translation: BAE29841.1.
BC029111 mRNA. Translation: AAH29111.1.
CCDSiCCDS23201.1. [Q8K2Z8-1]
RefSeqiNP_850931.2. NM_180600.3. [Q8K2Z8-1]
XP_006510831.1. XM_006510768.2. [Q8K2Z8-2]
UniGeneiMm.207894.

3D structure databases

ProteinModelPortaliQ8K2Z8.
SMRiQ8K2Z8. Positions 204-365.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000059798.

PTM databases

PhosphoSiteiQ8K2Z8.

Proteomic databases

MaxQBiQ8K2Z8.
PaxDbiQ8K2Z8.
PRIDEiQ8K2Z8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000059555; ENSMUSP00000059798; ENSMUSG00000032307. [Q8K2Z8-1]
ENSMUST00000122441; ENSMUSP00000112745; ENSMUSG00000032307. [Q8K2Z8-2]
GeneIDi109161.
KEGGimmu:109161.
UCSCiuc009psb.2. mouse. [Q8K2Z8-1]

Organism-specific databases

CTDi92912.
MGIiMGI:2388672. Ube2q2.

Phylogenomic databases

eggNOGiNOG320521.
GeneTreeiENSGT00390000018573.
HOGENOMiHOG000007757.
HOVERGENiHBG061368.
InParanoidiQ8K2Z8.
KOiK10582.
OMAiSPLRCEQ.
OrthoDBiEOG76DTSS.
PhylomeDBiQ8K2Z8.
TreeFamiTF313338.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

NextBioi361712.
PROiQ8K2Z8.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K2Z8.
CleanExiMM_UBE2Q2.
ExpressionAtlasiQ8K2Z8. baseline and differential.
GenevestigatoriQ8K2Z8.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR006575. RWD-domain.
IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF05773. RWD. 1 hit.
PF00179. UQ_con. 1 hit.
[Graphical view]
SMARTiSM00591. RWD. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 2 hits.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Bone marrow, Fetal head, Hippocampus and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-235.
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiUB2Q2_MOUSE
AccessioniPrimary (citable) accession number: Q8K2Z8
Secondary accession number(s): Q3UBX3
, Q3V3A5, Q8BUN2, Q8BVX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2006
Last sequence update: February 20, 2006
Last modified: March 31, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.