Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Condensin complex subunit 1

Gene

Ncapd2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. May target the condensin complex to DNA via its C-terminal domain (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA condensation, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Condensin complex subunit 1
Alternative name(s):
Chromosome condensation-related SMC-associated protein 1
Chromosome-associated protein D2
Short name:
mCAP-D2
Non-SMC condensin I complex subunit D2
XCAP-D2 homolog
Gene namesi
Name:Ncapd2
Synonyms:Capd2, Cnap1, Kiaa0159
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1915548. Ncapd2.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity
  • Chromosome By similarity

  • Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDK1, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase (By similarity).By similarity

GO - Cellular componenti

  • condensed chromosome Source: MGI
  • condensed chromosome, centromeric region Source: GO_Central
  • condensin complex Source: MGI
  • cytoplasm Source: MGI
  • membrane Source: MGI
  • nuclear condensin complex Source: GO_Central
  • nucleoplasm Source: MGI
  • pronucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000950361 – 1392Condensin complex subunit 1Add BLAST1392

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20PhosphoserineBy similarity1
Modified residuei575PhosphoserineBy similarity1
Modified residuei1300PhosphoserineCombined sources1
Modified residuei1305PhosphoserineBy similarity1
Modified residuei1320PhosphoserineCombined sources1
Modified residuei1323PhosphoserineCombined sources1
Modified residuei1329PhosphothreonineCombined sources1
Modified residuei1358PhosphoserineCombined sources1
Modified residuei1361PhosphoserineCombined sources1
Modified residuei1362PhosphoserineCombined sources1
Modified residuei1367PhosphoserineBy similarity1
Modified residuei1375Phosphothreonine; by CDK1By similarity1
Modified residuei1380Phosphothreonine; by CDK1By similarity1
Modified residuei1386PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPH and NCAPG subunits, activates the condensin complex and is required for chromosome condensation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8K2Z4.
PaxDbiQ8K2Z4.
PeptideAtlasiQ8K2Z4.
PRIDEiQ8K2Z4.

PTM databases

iPTMnetiQ8K2Z4.
PhosphoSitePlusiQ8K2Z4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000038252.
CleanExiMM_NCAPD2.

Interactioni

Subunit structurei

Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: NCAPH/BRRN1, NCAPD2/CAPD2 and NCAPG. Interacts with histones H1 and H3 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212791. 2 interactors.
STRINGi10090.ENSMUSP00000042260.

Structurei

3D structure databases

ProteinModelPortaliQ8K2Z4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 593Interaction with SMC2 and SMC4By similarityAdd BLAST593

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1332 – 1353Bipartite nuclear localization signalBy similarityAdd BLAST22

Domaini

The C-terminal domain interacts with histones H1 and H3, and may be responsible for condensin complex targeting to mitotic chromosomes. This domain is independent from the bipartite nuclear localization signal, although they are contained within the same region (By similarity).By similarity

Sequence similaritiesi

Belongs to the CND1 (condensin subunit 1) family.Curated

Phylogenomic databases

eggNOGiKOG0414. Eukaryota.
COG5098. LUCA.
HOVERGENiHBG038048.
InParanoidiQ8K2Z4.
KOiK06677.
PhylomeDBiQ8K2Z4.
TreeFamiTF105712.

Family and domain databases

Gene3Di1.25.10.10. 5 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR026971. CND1/NCAPD3.
IPR032682. Cnd1_C.
IPR007673. Condensin_cplx_su1.
IPR024324. Condensin_cplx_su1_N.
[Graphical view]
PANTHERiPTHR14222. PTHR14222. 2 hits.
PfamiPF12717. Cnd1. 1 hit.
PF12922. Cnd1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF017127. Condensin_D2. 1 hit.
SUPFAMiSSF48371. SSF48371. 5 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K2Z4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSPHNFEFHL PLSPEELLKS GGVNQYVVRE VLPVKHLSSQ LRAFQSAFRA
60 70 80 90 100
QGPLAILEHF DTVYSILHHF RSIEPGLKED TLEFLKKVVS RHSQELSSIL
110 120 130 140 150
DDAALSGSDR SAHLNALKMN CYALIRLLES FENMTSQTSL IDLDIGGKGK
160 170 180 190 200
RARAKATLGF DWEEERQPVL QLLTQLLQLD IRHLWNHSAI EEEFVSLVTG
210 220 230 240 250
CCYRLLENPT ISHQKNRSTK EAIAHLLGVA LVRYNHMLSA TVKIIQMLQH
260 270 280 290 300
FEHLPPVLVT AVSLWATDYG MKSIVGEIVR EIGQKCPQEL SRDTAGAKGF
310 320 330 340 350
AAFLTELAER IPAVLMANMC ILLDHLDGEN YMMRNAVLAA IAEMVLQVLN
360 370 380 390 400
GDQLEESARE TRDQFLDILQ AHGHDVNSFV RSRVLQLFAR IVQQKALPLT
410 420 430 440 450
RFQAVVALAV GRLADKSVLV CKNAIQLLAS FLANNPFSCK LSDIDLAGPL
460 470 480 490 500
QKEIQKLQEM RAQRRSAAAT AALDPEEEWD AMLPELKSTL QQLLKLPQEE
510 520 530 540 550
GDHQIADAET AEEVKGRIRQ LLAKASYKQA IVLTREATSH FQESEPFSHT
560 570 580 590 600
EPEENSFLNL LGLIFKGPEA STQDSHGDTD PGLTGSKDSP SVPEPEGSQS
610 620 630 640 650
NDELVKQEML VQYLQDAYGF SQKITEAIGI ISKMMYENTT TVVQEVIEFF
660 670 680 690 700
VMVFQFGVPQ ALFGVRRMLP LIWSKEPGVR EAVLNAYRQL YLNPKGDSAR
710 720 730 740 750
AKAQTLIHNL SLLLVDASVG TIQCLEEILC EFVQKDEVKP AVIQLLWERA
760 770 780 790 800
TEKVPSSPLE RCSSVMLLGM MARGKPEIVG SNLDALVRVG LDEKSPQDYR
810 820 830 840 850
LAQQVCLAIA NISDRRKPSL GERHPPFRLP QEHRLFERLQ DMVTKGFAHP
860 870 880 890 900
DPLWIPFKEV AVTLTYQLAE SPDVLCAQML QGCAKQVLEK LEKNATEADP
910 920 930 940 950
KETAPRLPTF LLMNLLSLAG DVALQQLVHL EQAVSGELGR RRVLREEQEH
960 970 980 990 1000
RAKEPKEKTA SSETTMEEEL GLVGGATADD TEAELIRSIC EKELLDGNQV
1010 1020 1030 1040 1050
LAAFVPLLLK VCNNPGLYSN PELCAAASLA LGKFCMISAP FCDSQLRLLF
1060 1070 1080 1090 1100
TMLEKSSLPT VRSNLMVATG DLAIRFPNLV DPWTPHLYAR LRDPAQQVRK
1110 1120 1130 1140 1150
TAGLVMTHLI LKDMVKVKGQ VSEMAVLLID PVPQIAALAK NFFNELSHKG
1160 1170 1180 1190 1200
NAIYNLLPDI ISRLSDPEGG VEEEPFHTIM KQLLSYITKD KQTESLVEKL
1210 1220 1230 1240 1250
CQRFRTARTE RQYRDLAYCM SQLPLTERGL QKMLDNFECF GDKLLDESVF
1260 1270 1280 1290 1300
SAFLSIVGKL RRGAKPEGKA IIDEFEQKLR ACHTRGMDGI EEFETGQGGS
1310 1320 1330 1340 1350
QRALSAKKPS AVSRLQPLTS VDSDNDFVTP KPRRTKPGRP QTQQRKKSQR
1360 1370 1380 1390
KAKVVFLSDE SSEDELSAEM TEEETPKRTT PIRRASGRRH RS
Length:1,392
Mass (Da):155,665
Last modified:April 23, 2003 - v2
Checksum:i192E117CC7172A87
GO
Isoform 2 (identifier: Q8K2Z4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1382-1392: IRRASGRRHRS → TLVEVLVVCGFNMEMAFALRSFS

Note: No experimental confirmation available.
Show »
Length:1,404
Mass (Da):156,878
Checksum:i717D40C6778889AF
GO

Sequence cautioni

The sequence AAH06673 differs from that shown. Contaminating sequence.Curated
The sequence BAC97878 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BC025460 differs from that shown. Reason: Erroneous termination at position 1245. Translated as Leu.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti574D → G in AAH26982 (PubMed:15489334).Curated1
Sequence conflicti1256I → V in AAH26982 (PubMed:15489334).Curated1
Sequence conflicti1256I → V in BAB31633 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0072461382 – 1392IRRASGRRHRS → TLVEVLVVCGFNMEMAFALR SFS in isoform 2. 1 PublicationAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129068 mRNA. Translation: BAC97878.1. Different initiation.
BC006673 mRNA. Translation: AAH06673.1. Sequence problems.
BC008592 mRNA. Translation: AAH08592.1.
BC025460 mRNA. No translation available.
BC026982 mRNA. Translation: AAH26982.1.
BC029133 mRNA. Translation: AAH29133.1.
AK011352 mRNA. Translation: BAB27560.1.
AK019259 mRNA. Translation: BAB31633.1.
CCDSiCCDS39636.1. [Q8K2Z4-1]
RefSeqiNP_666283.1. NM_146171.1.
XP_006506594.1. XM_006506531.3.
UniGeneiMm.257590.

Genome annotation databases

GeneIDi68298.
KEGGimmu:68298.
UCSCiuc009dtt.1. mouse. [Q8K2Z4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129068 mRNA. Translation: BAC97878.1. Different initiation.
BC006673 mRNA. Translation: AAH06673.1. Sequence problems.
BC008592 mRNA. Translation: AAH08592.1.
BC025460 mRNA. No translation available.
BC026982 mRNA. Translation: AAH26982.1.
BC029133 mRNA. Translation: AAH29133.1.
AK011352 mRNA. Translation: BAB27560.1.
AK019259 mRNA. Translation: BAB31633.1.
CCDSiCCDS39636.1. [Q8K2Z4-1]
RefSeqiNP_666283.1. NM_146171.1.
XP_006506594.1. XM_006506531.3.
UniGeneiMm.257590.

3D structure databases

ProteinModelPortaliQ8K2Z4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212791. 2 interactors.
STRINGi10090.ENSMUSP00000042260.

PTM databases

iPTMnetiQ8K2Z4.
PhosphoSitePlusiQ8K2Z4.

Proteomic databases

EPDiQ8K2Z4.
PaxDbiQ8K2Z4.
PeptideAtlasiQ8K2Z4.
PRIDEiQ8K2Z4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi68298.
KEGGimmu:68298.
UCSCiuc009dtt.1. mouse. [Q8K2Z4-1]

Organism-specific databases

CTDi9918.
MGIiMGI:1915548. Ncapd2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0414. Eukaryota.
COG5098. LUCA.
HOVERGENiHBG038048.
InParanoidiQ8K2Z4.
KOiK06677.
PhylomeDBiQ8K2Z4.
TreeFamiTF105712.

Miscellaneous databases

ChiTaRSiNcapd2. mouse.
PROiQ8K2Z4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038252.
CleanExiMM_NCAPD2.

Family and domain databases

Gene3Di1.25.10.10. 5 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR026971. CND1/NCAPD3.
IPR032682. Cnd1_C.
IPR007673. Condensin_cplx_su1.
IPR024324. Condensin_cplx_su1_N.
[Graphical view]
PANTHERiPTHR14222. PTHR14222. 2 hits.
PfamiPF12717. Cnd1. 1 hit.
PF12922. Cnd1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF017127. Condensin_D2. 1 hit.
SUPFAMiSSF48371. SSF48371. 5 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCND1_MOUSE
AccessioniPrimary (citable) accession number: Q8K2Z4
Secondary accession number(s): Q6ZQI1
, Q8R0F2, Q8R3H0, Q922B7, Q923A3, Q9CS25, Q9CT32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: April 23, 2003
Last modified: November 2, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.