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Protein

CST complex subunit STN1

Gene

Obfc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere duplex. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. Initially the CST complex has been proposed to protect telomeres from DNA degradation (PubMed:19854130). However, the CST complex has been shown to be involved in several aspects of telomere replication. The CST complex inhibits telomerase and is involved in telomere length homeostasis; it is proposed to bind to newly telomerase-synthesized 3' overhangs and to terminate telomerase action implicating the association with the ACD:POT1 complex thus interfering with its telomerase stimulation activity. The CST complex is also proposed to be involved in fill-in synthesis of the telomeric C-strand probably implicating recruitment and activation of DNA polymerase alpha (PubMed:22748632). The CST complex facilitates recovery from many forms of exogenous DNA damage; seems to be involved in the re-initiation of DNA replication at repaired forks and/or dormant origins. Required for efficicient replication of the duplex region of the telomere. Promotes efficient replication of lagging-strand telomeres. Promotes general replication start following replication-fork stalling implicating new origin firing. May be in involved in C-strand fill-in during late S/G2 phase independent of ot its role in telomere duplex replication (By similarity).By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi64 – 165102OBAdd
BLAST

GO - Molecular functioni

  • single-stranded DNA binding Source: UniProtKB
  • single-stranded telomeric DNA binding Source: UniProtKB
  • telomerase inhibitor activity Source: Ensembl
  • telomeric DNA binding Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CST complex subunit STN1
Alternative name(s):
Alpha-accessory factor of 44 kDa
Short name:
AAF-44
Short name:
AAF44
Oligonucleotide/oligosaccharide-binding fold-containing protein 1
Suppressor of cdc thirteen homolog
Gene namesi
Name:Obfc1
Synonyms:Stn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1915581. Obfc1.

Subcellular locationi

GO - Cellular componenti

  • CST complex Source: UniProtKB
  • intermediate filament cytoskeleton Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • nuclear chromosome, telomeric region Source: UniProtKB
  • nucleolus Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi96 – 961W → A: Impairs single-stranded DNA binding; when associated with A-151. 1 Publication
Mutagenesisi151 – 1511F → A: Impairs single-stranded DNA binding; when associated with A-96. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 378377CST complex subunit STN1PRO_0000058021Add
BLAST

Proteomic databases

EPDiQ8K2X3.
MaxQBiQ8K2X3.
PaxDbiQ8K2X3.
PeptideAtlasiQ8K2X3.
PRIDEiQ8K2X3.

PTM databases

iPTMnetiQ8K2X3.
PhosphoSiteiQ8K2X3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000042694.
ExpressionAtlasiQ8K2X3. baseline and differential.
GenevisibleiQ8K2X3. MM.

Interactioni

Subunit structurei

Component of the CST complex, composed of TEN1/C17orf106, CTC1/C17orf68 and STN1/OBFC1; in the complex interacts directly with TEN1 and CTC1 (PubMed:19119139, PubMed:19854130). Interacts with ACD/TPP1 (PubMed:19648609). Interacts with POT1 and POLA1 (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AcdQ5EE382EBI-2553883,EBI-6258642

Protein-protein interaction databases

BioGridi224373. 3 interactions.
IntActiQ8K2X3. 7 interactions.
STRINGi10090.ENSMUSP00000040944.

Structurei

Secondary structure

1
378
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi211 – 22919Combined sources
Helixi236 – 2405Combined sources
Helixi243 – 2497Combined sources
Helixi271 – 28717Combined sources
Beta strandi289 – 2913Combined sources
Beta strandi296 – 2983Combined sources
Beta strandi301 – 3033Combined sources
Beta strandi305 – 3073Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WJ5NMR-A205-311[»]
ProteinModelPortaliQ8K2X3.
SMRiQ8K2X3. Positions 31-192, 201-378.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8K2X3.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni8 – 195188Interaction with CTC1By similarityAdd
BLAST
Regioni201 – 305105Winged helix-turn-helix (wHTH) 1By similarityAdd
BLAST
Regioni306 – 37873Winged helix-turn-helix (wHTH) 2By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the STN1 family.Curated
Contains 1 OB DNA-binding domain.Curated

Phylogenomic databases

eggNOGiKOG3108. Eukaryota.
COG5235. LUCA.
GeneTreeiENSGT00390000000909.
HOGENOMiHOG000088638.
HOVERGENiHBG053364.
InParanoidiQ8K2X3.
OMAiQTFYQQE.
OrthoDBiEOG091G0A0K.
PhylomeDBiQ8K2X3.
TreeFamiTF328623.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.40.50.140. 1 hit.
InterProiIPR015253. CST_STN1_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
IPR014647. Stn1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR13989:SF14. PTHR13989:SF14. 1 hit.
PfamiPF09170. STN1_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PIRSFiPIRSF036950. UCP036950. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50249. SSF50249. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K2X3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQPCLLMEC ESSPREEEIP PLFWGLDPVF LAFAKLYIKD ILEMKESQQV
60 70 80 90 100
PGTYFYNGHP IRRVDIMGAV ISVKERETFY SYGVDDATGV INCVCWKKLS
110 120 130 140 150
NAESSSDPAI LSTARELSMT SQLKKLQETI EQKTRIGIGD IIRVRGSVRM
160 170 180 190 200
FREEREICAN IYYKVDDPVW NMQIARMLEL PKLYQKVYDQ PFRNPALQEE
210 220 230 240 250
EALNNKDNLD LAGLTSLLSE KIKEFLQEKK MQSFYQQELE TVESLQSLAS
260 270 280 290 300
RPVTHSTGSD QVELKDSGTS GVAQRVFKNA LQLLQEKGLV FQRDSGSDKL
310 320 330 340 350
YYVTTKDKDL QQKIYHIIKE DCQKPNHMEK GCHLLHILNC VHLNLRWDLS
360 370
KAVLQRVLEL LEDQSDIVST ADHYYAAF
Length:378
Mass (Da):43,486
Last modified:March 23, 2010 - v2
Checksum:i23C7FC13A10C6F1F
GO

Sequence cautioni

The sequence AAH29548 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti339 – 3391N → K in AAH29548 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075574 mRNA. Translation: BAC35830.1.
AK146929 mRNA. Translation: BAE27540.1.
AK164386 mRNA. Translation: BAE37766.1.
CH466534 Genomic DNA. Translation: EDL42035.1.
BC029548 mRNA. Translation: AAH29548.1. Different initiation.
CCDSiCCDS29892.1.
RefSeqiNP_780569.1. NM_175360.2.
XP_006526660.1. XM_006526597.2.
UniGeneiMm.218048.

Genome annotation databases

EnsembliENSMUST00000049369; ENSMUSP00000040944; ENSMUSG00000042694.
GeneIDi108689.
KEGGimmu:108689.
UCSCiuc008hvb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075574 mRNA. Translation: BAC35830.1.
AK146929 mRNA. Translation: BAE27540.1.
AK164386 mRNA. Translation: BAE37766.1.
CH466534 Genomic DNA. Translation: EDL42035.1.
BC029548 mRNA. Translation: AAH29548.1. Different initiation.
CCDSiCCDS29892.1.
RefSeqiNP_780569.1. NM_175360.2.
XP_006526660.1. XM_006526597.2.
UniGeneiMm.218048.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WJ5NMR-A205-311[»]
ProteinModelPortaliQ8K2X3.
SMRiQ8K2X3. Positions 31-192, 201-378.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi224373. 3 interactions.
IntActiQ8K2X3. 7 interactions.
STRINGi10090.ENSMUSP00000040944.

PTM databases

iPTMnetiQ8K2X3.
PhosphoSiteiQ8K2X3.

Proteomic databases

EPDiQ8K2X3.
MaxQBiQ8K2X3.
PaxDbiQ8K2X3.
PeptideAtlasiQ8K2X3.
PRIDEiQ8K2X3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049369; ENSMUSP00000040944; ENSMUSG00000042694.
GeneIDi108689.
KEGGimmu:108689.
UCSCiuc008hvb.1. mouse.

Organism-specific databases

CTDi79991.
MGIiMGI:1915581. Obfc1.

Phylogenomic databases

eggNOGiKOG3108. Eukaryota.
COG5235. LUCA.
GeneTreeiENSGT00390000000909.
HOGENOMiHOG000088638.
HOVERGENiHBG053364.
InParanoidiQ8K2X3.
OMAiQTFYQQE.
OrthoDBiEOG091G0A0K.
PhylomeDBiQ8K2X3.
TreeFamiTF328623.

Miscellaneous databases

EvolutionaryTraceiQ8K2X3.
PROiQ8K2X3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042694.
ExpressionAtlasiQ8K2X3. baseline and differential.
GenevisibleiQ8K2X3. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.40.50.140. 1 hit.
InterProiIPR015253. CST_STN1_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
IPR014647. Stn1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR13989:SF14. PTHR13989:SF14. 1 hit.
PfamiPF09170. STN1_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PIRSFiPIRSF036950. UCP036950. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50249. SSF50249. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiSTN1_MOUSE
AccessioniPrimary (citable) accession number: Q8K2X3
Secondary accession number(s): Q3TPH0, Q8C6I6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: March 23, 2010
Last modified: September 7, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

According to PubMed:19119139, it acts as a regulator of DNA replication. According to PubMed:19854130, this effect is indirect and it rather acts as a general regulator of DNA metabolism.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.