Q8K2X1 (AT10D_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable phospholipid-transporting ATPase VD
EC=3.6.3.1

Alternative name(s):
ATPase class V type 10D

Gene names

Name:

Atp10d

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1416 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out).
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Expressed in placenta and kidney. Ref.1
Polymorphism	In strain C57BL/6, a polymorphism generates a premature stop codon at position 764. Ref.1
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. [View classification]
Sequence caution	The sequence AAH29551.1 differs from that shown. Reason: Frameshift at position 1136.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cation transport Non-traceable author statement Ref.1. Source: UniProtKB
Cellular component	integral to membrane Non-traceable author statement Ref.1. Source: UniProtKB mitochondrion Inferred from direct assay. Source: MGI
Molecular function	ATP binding Non-traceable author statement Ref.1. Source: UniProtKB ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Inferred from electronic annotation. Source: InterPro magnesium ion binding Non-traceable author statement Ref.1. Source: UniProtKB phospholipid-translocating ATPase activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8K2X1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8K2X1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1120-1135: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1416	1416	Probable phospholipid-transporting ATPase VD		PRO_0000046384
Regions
	Topological domain	1 – 97	97	Cytoplasmic Potential
	Transmembrane	98 – 118	21	Helical; Potential
	Topological domain	119 – 120	2	Extracellular Potential
	Transmembrane	121 – 141	21	Helical; Potential
	Topological domain	142 – 321	180	Cytoplasmic Potential
	Transmembrane	322 – 342	21	Helical; Potential
	Topological domain	343 – 365	23	Extracellular Potential
	Transmembrane	366 – 386	21	Helical; Potential
	Topological domain	387 – 1110	724	Cytoplasmic Potential
	Transmembrane	1111 – 1131	21	Helical; Potential
	Topological domain	1132 – 1142	11	Extracellular Potential
	Transmembrane	1143 – 1163	21	Helical; Potential
	Topological domain	1164 – 1192	29	Cytoplasmic Potential
	Transmembrane	1193 – 1213	21	Helical; Potential
	Topological domain	1214 – 1221	8	Extracellular Potential
	Transmembrane	1222 – 1242	21	Helical; Potential
	Topological domain	1243 – 1252	10	Cytoplasmic Potential
	Transmembrane	1253 – 1273	21	Helical; Potential
	Topological domain	1274 – 1289	16	Extracellular Potential
	Transmembrane	1290 – 1310	21	Helical; Potential
	Topological domain	1311 – 1416	106	Cytoplasmic Potential
	Nucleotide binding	993 – 1000	8	ATP Potential
	Nucleotide binding	1361 – 1368	8	ATP Potential
Sites
	Active site	438	1	4-aspartylphosphate intermediate By similarity
	Metal binding	1053	1	Magnesium By similarity UniProtKB Q9P241
	Metal binding	1057	1	Magnesium By similarity UniProtKB Q9P241
Natural variations
	Alternative sequence	1120 – 1135	16	Missing in isoform 2.		VSP_006959
	Natural variant	700	1	V → A in strain: CAST, MAI, MBT and PWK. Ref.1
	Natural variant	716	1	S → L in strain: CAST, MAI, MBT and PWK. Ref.1
Experimental info
	Sequence conflict	1239	1	L → F in AAH29551. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 25, 2003. Version 2. Checksum: 1799CCF062E22BB0	FASTA	1,416	158,330
	10 20 30 40 50 60 MTELLQWARH HWRRLSHGRA QGEDERPYNY ASLLACGGKS SRTPRPAGKH RVVIPHLQCF 70 80 90 100 110 120 KDEYERFSGT YVNNRIRTTK YTLLNFVPRN LFEQFHRAAN LYFLFLVVLN WVPLVEAFQK 130 140 150 160 170 180 EITMLPLVVV LTIIAIKDGL EDYRKYKIDK QINNLITKVY SRKEKKYIDC CWKNVTVGDF 190 200 210 220 230 240 IRLSCNEIIP ADMVLLFSTD PDGICHIETS GLDGESNLKQ RQVVRGYTEQ DSEVDPEKFS 250 260 270 280 290 300 SRIECESPNN DLSRFRGFLE HANKERVGLS KENLLLRGCT IRNTEAVVGI VVYAGHETKA 310 320 330 340 350 360 MLNNSGPRYK RSKLERRANT DVLWCVLLLI VMCLTGALGH GIWLSRYENM LFFNIPEPDG 370 380 390 400 410 420 RVISPVLTGF YVFWTMIILL QVLIPISLYV SIEIVKLGQI YFIQSDVDFY NEKMDSTIQC 430 440 450 460 470 480 RALNITEDLG QIQYLFSDKT GTLTENKMVF RRCSVAGFDY CHEENAKRLE SYQEAVSEEE 490 500 510 520 530 540 ECTDTLGGSL SNMARPRAQG CRTVPSGPLG KPSAQLSGST SAVGNGEGSG EVPHSRQAAF 550 560 570 580 590 600 SSPMETDVVP DTRLLDKFSQ LTPQLLTGLD GTAQSSPLET LYIMDFFIAL AICNTVVVSA 610 620 630 640 650 660 PNQPRQKIGL SSLGGMPIKS LEEIKNIFQK LSVRRSSSPS LASGKDSSSG TPCAFVSRIS 670 680 690 700 710 720 FFSRPKLSPP MEDESSQMDE IPQASNSACC TETEAQNRAV GLSVSSAEAL SGPPPSASNL 730 740 750 760 770 780 CYEAESPDEA ALVYAARAYR CTLQSRTPEQ VMVDFAALGS LTFQLLHILP FDSVRKRMSV 790 800 810 820 830 840 VVRHPLSKQV VVYTKGADSV IMELLSVAAS DGTNPEQQMI IRERTQRHLD EYAKRGLRTL 850 860 870 880 890 900 CVAKKVMSDT EYAEWLRNHF LAETSIDNRE ELLVESAMRL ENKLTLLGAT GIEDRLQEGV 910 920 930 940 950 960 PESIEALHQA GIKIWMLTGD KQETAVNIAY ACKLLEPDDK LFILNTQSQD ACGMLMSAIL 970 980 990 1000 1010 1020 EELQKRAQVS PELASSRKNF PQPSDAQGQG RAGLVITGKT LEFALQESLQ RQFLELTAWC 1030 1040 1050 1060 1070 1080 QAVICCRATP LQKSEVVKLV RNHHHVLTLP IGDGANDVSM IQVADIGIGV SGQEGMQAVM 1090 1100 1110 1120 1130 1140 ASDFAISQFR HLSKLLLVHG HWCYTRLSNM ILYFFYKNVA YVNLLFWYQF FCGFSGTSMT 1150 1160 1170 1180 1190 1200 DYWVLIFFNL LFTSVPPIIY GVLEKDVSAE TLLQLPELYR SGQRSEEYLP LTFWITLLDA 1210 1220 1230 1240 1250 1260 FYQSLVCFFV PYFTYQGSDI DIFTFGNPLN TAALFIILLH LVIESKSLTW IHMLVTVGSI 1270 1280 1290 1300 1310 1320 LSYFFFALAF GALCVTCNPP SNPYGIMRKH MLDPVFYLVC VLTTFVALLP RFLYRVLQGS 1330 1340 1350 1360 1370 1380 VFPSPVLRAK YFDRLPPEER AEALKRWRGT AKVNHVASKH ASQSAAMSGR PTPGSSAVLA 1390 1400 1410 MKSATVSTVE QSTRETALDR GCSEPGASKM TGSSAS
	Isoform 2 [UniParc]. Checksum: 0694AA41D4A881A5	FASTA	1,400	156,343
	10 20 30 40 50 60 MTELLQWARH HWRRLSHGRA QGEDERPYNY ASLLACGGKS SRTPRPAGKH RVVIPHLQCF 70 80 90 100 110 120 KDEYERFSGT YVNNRIRTTK YTLLNFVPRN LFEQFHRAAN LYFLFLVVLN WVPLVEAFQK 130 140 150 160 170 180 EITMLPLVVV LTIIAIKDGL EDYRKYKIDK QINNLITKVY SRKEKKYIDC CWKNVTVGDF 190 200 210 220 230 240 IRLSCNEIIP ADMVLLFSTD PDGICHIETS GLDGESNLKQ RQVVRGYTEQ DSEVDPEKFS 250 260 270 280 290 300 SRIECESPNN DLSRFRGFLE HANKERVGLS KENLLLRGCT IRNTEAVVGI VVYAGHETKA 310 320 330 340 350 360 MLNNSGPRYK RSKLERRANT DVLWCVLLLI VMCLTGALGH GIWLSRYENM LFFNIPEPDG 370 380 390 400 410 420 RVISPVLTGF YVFWTMIILL QVLIPISLYV SIEIVKLGQI YFIQSDVDFY NEKMDSTIQC 430 440 450 460 470 480 RALNITEDLG QIQYLFSDKT GTLTENKMVF RRCSVAGFDY CHEENAKRLE SYQEAVSEEE 490 500 510 520 530 540 ECTDTLGGSL SNMARPRAQG CRTVPSGPLG KPSAQLSGST SAVGNGEGSG EVPHSRQAAF 550 560 570 580 590 600 SSPMETDVVP DTRLLDKFSQ LTPQLLTGLD GTAQSSPLET LYIMDFFIAL AICNTVVVSA 610 620 630 640 650 660 PNQPRQKIGL SSLGGMPIKS LEEIKNIFQK LSVRRSSSPS LASGKDSSSG TPCAFVSRIS 670 680 690 700 710 720 FFSRPKLSPP MEDESSQMDE IPQASNSACC TETEAQNRAV GLSVSSAEAL SGPPPSASNL 730 740 750 760 770 780 CYEAESPDEA ALVYAARAYR CTLQSRTPEQ VMVDFAALGS LTFQLLHILP FDSVRKRMSV 790 800 810 820 830 840 VVRHPLSKQV VVYTKGADSV IMELLSVAAS DGTNPEQQMI IRERTQRHLD EYAKRGLRTL 850 860 870 880 890 900 CVAKKVMSDT EYAEWLRNHF LAETSIDNRE ELLVESAMRL ENKLTLLGAT GIEDRLQEGV 910 920 930 940 950 960 PESIEALHQA GIKIWMLTGD KQETAVNIAY ACKLLEPDDK LFILNTQSQD ACGMLMSAIL 970 980 990 1000 1010 1020 EELQKRAQVS PELASSRKNF PQPSDAQGQG RAGLVITGKT LEFALQESLQ RQFLELTAWC 1030 1040 1050 1060 1070 1080 QAVICCRATP LQKSEVVKLV RNHHHVLTLP IGDGANDVSM IQVADIGIGV SGQEGMQAVM 1090 1100 1110 1120 1130 1140 ASDFAISQFR HLSKLLLVHG HWCYTRLSNM ILYFFYKNVG TSMTDYWVLI FFNLLFTSVP 1150 1160 1170 1180 1190 1200 PIIYGVLEKD VSAETLLQLP ELYRSGQRSE EYLPLTFWIT LLDAFYQSLV CFFVPYFTYQ 1210 1220 1230 1240 1250 1260 GSDIDIFTFG NPLNTAALFI ILLHLVIESK SLTWIHMLVT VGSILSYFFF ALAFGALCVT 1270 1280 1290 1300 1310 1320 CNPPSNPYGI MRKHMLDPVF YLVCVLTTFV ALLPRFLYRV LQGSVFPSPV LRAKYFDRLP 1330 1340 1350 1360 1370 1380 PEERAEALKR WRGTAKVNHV ASKHASQSAA MSGRPTPGSS AVLAMKSATV STVEQSTRET 1390 1400 ALDRGCSEPG ASKMTGSSAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a putative type IV aminophospholipid transporter P-type ATPase." Flamant S., Pescher P., Lemercier B., Clement-Ziza M., Kepes F., Fellous M., Milon G., Marchal G., Besmond C. Mamm. Genome 14:21-30(2003) [PubMed: 12532265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, VARIANTS ALA-700 AND LEU-716. Strain: BALB/c, C57BL/6, CAST, MAI, MBT and PWK. Tissue: Monocyte.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1079-1416 (ISOFORM 2).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ441079 mRNA. Translation: CAD29578.1. BC029551 mRNA. Translation: AAH29551.1. Frameshift.
IPI	IPI00170015. IPI00227819.
RefSeq	NP_700438.3. NM_153389.3.
UniGene	Mm.32775. Mm.441066.
3D structure databases
ProteinModelPortal	Q8K2X1.
SMR	Q8K2X1. Positions 1024-1088.
ModBase	Search...
PTM databases
PhosphoSite	Q8K2X1.
Proteomic databases
PRIDE	Q8K2X1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	231287.
KEGG	mmu:231287.
UCSC	uc008xrh.2. mouse.
Organism-specific databases
CTD	57205.
MGI	MGI:2450125. Atp10d.
Phylogenomic databases
HOGENOM	HBG745019.
HOVERGEN	HBG107129.
InParanoid	Q8K2X1.
OrthoDB	EOG45DWNJ.
PhylomeDB	Q8K2X1.
Gene expression databases
ArrayExpress	Q8K2X1.
CleanEx	MM_ATP10D.
Genevestigator	Q8K2X1.
GermOnline	ENSMUSG00000046808. Mus musculus.
Family and domain databases
InterPro	IPR023306. ATPase_cation_domN. IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR023300. ATPase_P-typ_cyto_domA. IPR023299. ATPase_P-typ_cyto_domN. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR006539. ATPase_P-typ_Plipid-transl. IPR023214. HAD-like_dom. [Graphical view]
Gene3D	G3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 2 hits. G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 3 hits. G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
KO	K01530.
Pfam	PF00122. E1-E2_ATPase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
SUPFAM	SSF81660. ATPase_cation_domN. 1 hit. SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01652. ATPase-Plipid. 2 hits. TIGR01494. ATPase_P-type. 2 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	380484.
SOURCE	Search...

Entry information

Entry name

AT10D_MOUSE

Accession

Primary (citable) accession number: Q8K2X1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	March 25, 2003
Last modified:	December 14, 2011
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program