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Protein

Probable phospholipid-transporting ATPase VD

Gene

Atp10d

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules (By similarity).By similarity

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei438 – 43814-aspartylphosphate intermediateBy similarity
Metal bindingi1053 – 10531MagnesiumBy similarity
Metal bindingi1057 – 10571MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi993 – 10008ATPCurated
Nucleotide bindingi1361 – 13688ATPCurated

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • phospholipid-translocating ATPase activity Source: UniProtKB

GO - Biological processi

  • cation transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phospholipid-transporting ATPase VD (EC:3.6.3.1)
Alternative name(s):
ATPase class V type 10D
P4-ATPase flippase complex alpha subunit ATP10D
Gene namesi
Name:Atp10d
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2450125. Atp10d.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9797CytoplasmicSequence analysisAdd
BLAST
Transmembranei98 – 11821HelicalSequence analysisAdd
BLAST
Topological domaini119 – 1202Exoplasmic loopSequence analysis
Transmembranei121 – 14121HelicalSequence analysisAdd
BLAST
Topological domaini142 – 321180CytoplasmicSequence analysisAdd
BLAST
Transmembranei322 – 34221HelicalSequence analysisAdd
BLAST
Topological domaini343 – 36523Exoplasmic loopSequence analysisAdd
BLAST
Transmembranei366 – 38621HelicalSequence analysisAdd
BLAST
Topological domaini387 – 1110724CytoplasmicSequence analysisAdd
BLAST
Transmembranei1111 – 113121HelicalSequence analysisAdd
BLAST
Topological domaini1132 – 114211Exoplasmic loopSequence analysisAdd
BLAST
Transmembranei1143 – 116321HelicalSequence analysisAdd
BLAST
Topological domaini1164 – 119229CytoplasmicSequence analysisAdd
BLAST
Transmembranei1193 – 121321HelicalSequence analysisAdd
BLAST
Topological domaini1214 – 12218Exoplasmic loopSequence analysis
Transmembranei1222 – 124221HelicalSequence analysisAdd
BLAST
Topological domaini1243 – 125210CytoplasmicSequence analysis
Transmembranei1253 – 127321HelicalSequence analysisAdd
BLAST
Topological domaini1274 – 128916Exoplasmic loopSequence analysisAdd
BLAST
Transmembranei1290 – 131021HelicalSequence analysisAdd
BLAST
Topological domaini1311 – 1416106CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14161416Probable phospholipid-transporting ATPase VDPRO_0000046384Add
BLAST

Proteomic databases

MaxQBiQ8K2X1.
PRIDEiQ8K2X1.

PTM databases

iPTMnetiQ8K2X1.
PhosphoSiteiQ8K2X1.

Expressioni

Tissue specificityi

Expressed in placenta and kidney.1 Publication

Gene expression databases

CleanExiMM_ATP10D.

Interactioni

Subunit structurei

Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit and an accessory beta subunit.By similarity

Protein-protein interaction databases

MINTiMINT-4997745.

Structurei

3D structure databases

ProteinModelPortaliQ8K2X1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG107129.
InParanoidiQ8K2X1.
KOiK01530.
PhylomeDBiQ8K2X1.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 3 hits.
3.40.50.1000. 2 hits.
InterProiIPR030360. ATP10D.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 3 hits.
PTHR24092:SF84. PTHR24092:SF84. 3 hits.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 2 hits.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1Curated (identifier: Q8K2X1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTELLQWARH HWRRLSHGRA QGEDERPYNY ASLLACGGKS SRTPRPAGKH
60 70 80 90 100
RVVIPHLQCF KDEYERFSGT YVNNRIRTTK YTLLNFVPRN LFEQFHRAAN
110 120 130 140 150
LYFLFLVVLN WVPLVEAFQK EITMLPLVVV LTIIAIKDGL EDYRKYKIDK
160 170 180 190 200
QINNLITKVY SRKEKKYIDC CWKNVTVGDF IRLSCNEIIP ADMVLLFSTD
210 220 230 240 250
PDGICHIETS GLDGESNLKQ RQVVRGYTEQ DSEVDPEKFS SRIECESPNN
260 270 280 290 300
DLSRFRGFLE HANKERVGLS KENLLLRGCT IRNTEAVVGI VVYAGHETKA
310 320 330 340 350
MLNNSGPRYK RSKLERRANT DVLWCVLLLI VMCLTGALGH GIWLSRYENM
360 370 380 390 400
LFFNIPEPDG RVISPVLTGF YVFWTMIILL QVLIPISLYV SIEIVKLGQI
410 420 430 440 450
YFIQSDVDFY NEKMDSTIQC RALNITEDLG QIQYLFSDKT GTLTENKMVF
460 470 480 490 500
RRCSVAGFDY CHEENAKRLE SYQEAVSEEE ECTDTLGGSL SNMARPRAQG
510 520 530 540 550
CRTVPSGPLG KPSAQLSGST SAVGNGEGSG EVPHSRQAAF SSPMETDVVP
560 570 580 590 600
DTRLLDKFSQ LTPQLLTGLD GTAQSSPLET LYIMDFFIAL AICNTVVVSA
610 620 630 640 650
PNQPRQKIGL SSLGGMPIKS LEEIKNIFQK LSVRRSSSPS LASGKDSSSG
660 670 680 690 700
TPCAFVSRIS FFSRPKLSPP MEDESSQMDE IPQASNSACC TETEAQNRAV
710 720 730 740 750
GLSVSSAEAL SGPPPSASNL CYEAESPDEA ALVYAARAYR CTLQSRTPEQ
760 770 780 790 800
VMVDFAALGS LTFQLLHILP FDSVRKRMSV VVRHPLSKQV VVYTKGADSV
810 820 830 840 850
IMELLSVAAS DGTNPEQQMI IRERTQRHLD EYAKRGLRTL CVAKKVMSDT
860 870 880 890 900
EYAEWLRNHF LAETSIDNRE ELLVESAMRL ENKLTLLGAT GIEDRLQEGV
910 920 930 940 950
PESIEALHQA GIKIWMLTGD KQETAVNIAY ACKLLEPDDK LFILNTQSQD
960 970 980 990 1000
ACGMLMSAIL EELQKRAQVS PELASSRKNF PQPSDAQGQG RAGLVITGKT
1010 1020 1030 1040 1050
LEFALQESLQ RQFLELTAWC QAVICCRATP LQKSEVVKLV RNHHHVLTLP
1060 1070 1080 1090 1100
IGDGANDVSM IQVADIGIGV SGQEGMQAVM ASDFAISQFR HLSKLLLVHG
1110 1120 1130 1140 1150
HWCYTRLSNM ILYFFYKNVA YVNLLFWYQF FCGFSGTSMT DYWVLIFFNL
1160 1170 1180 1190 1200
LFTSVPPIIY GVLEKDVSAE TLLQLPELYR SGQRSEEYLP LTFWITLLDA
1210 1220 1230 1240 1250
FYQSLVCFFV PYFTYQGSDI DIFTFGNPLN TAALFIILLH LVIESKSLTW
1260 1270 1280 1290 1300
IHMLVTVGSI LSYFFFALAF GALCVTCNPP SNPYGIMRKH MLDPVFYLVC
1310 1320 1330 1340 1350
VLTTFVALLP RFLYRVLQGS VFPSPVLRAK YFDRLPPEER AEALKRWRGT
1360 1370 1380 1390 1400
AKVNHVASKH ASQSAAMSGR PTPGSSAVLA MKSATVSTVE QSTRETALDR
1410
GCSEPGASKM TGSSAS
Length:1,416
Mass (Da):158,330
Last modified:March 25, 2003 - v2
Checksum:i1799CCF062E22BB0
GO
Isoform 2Curated (identifier: Q8K2X1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1120-1135: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:1,400
Mass (Da):156,343
Checksum:i0694AA41D4A881A5
GO

Sequence cautioni

The sequence AAH29551.1 differs from that shown. Reason: Frameshift at position 1136. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1239 – 12391L → F in AAH29551 (PubMed:15489334).Curated

Polymorphismi

In strain C57BL/6, a polymorphism generates a premature stop codon at position 764.1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti700 – 7001V → A in strain: CAST, MAI, MBT and PWK. 1 Publication
Natural varianti716 – 7161S → L in strain: CAST, MAI, MBT and PWK. 1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1120 – 113516Missing in isoform 2. 1 PublicationVSP_006959Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ441079 mRNA. Translation: CAD29578.1.
BC029551 mRNA. Translation: AAH29551.1. Frameshift.
RefSeqiNP_700438.3. NM_153389.3.
UniGeneiMm.32775.
Mm.441066.

Genome annotation databases

GeneIDi231287.
KEGGimmu:231287.
UCSCiuc008xrh.2. mouse. [Q8K2X1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ441079 mRNA. Translation: CAD29578.1.
BC029551 mRNA. Translation: AAH29551.1. Frameshift.
RefSeqiNP_700438.3. NM_153389.3.
UniGeneiMm.32775.
Mm.441066.

3D structure databases

ProteinModelPortaliQ8K2X1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4997745.

PTM databases

iPTMnetiQ8K2X1.
PhosphoSiteiQ8K2X1.

Proteomic databases

MaxQBiQ8K2X1.
PRIDEiQ8K2X1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi231287.
KEGGimmu:231287.
UCSCiuc008xrh.2. mouse. [Q8K2X1-1]

Organism-specific databases

CTDi57205.
MGIiMGI:2450125. Atp10d.

Phylogenomic databases

HOVERGENiHBG107129.
InParanoidiQ8K2X1.
KOiK01530.
PhylomeDBiQ8K2X1.

Miscellaneous databases

PROiQ8K2X1.
SOURCEiSearch...

Gene expression databases

CleanExiMM_ATP10D.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 3 hits.
3.40.50.1000. 2 hits.
InterProiIPR030360. ATP10D.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 3 hits.
PTHR24092:SF84. PTHR24092:SF84. 3 hits.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 2 hits.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a putative type IV aminophospholipid transporter P-type ATPase."
    Flamant S., Pescher P., Lemercier B., Clement-Ziza M., Kepes F., Fellous M., Milon G., Marchal G., Besmond C.
    Mamm. Genome 14:21-30(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, VARIANTS ALA-700 AND LEU-716.
    Strain: BALB/cJ, C57BL/6J, CAST/EiJ, MAI, MBT and PWK.
    Tissue: Monocyte.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1079-1416 (ISOFORM 2).

Entry informationi

Entry nameiAT10D_MOUSE
AccessioniPrimary (citable) accession number: Q8K2X1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: March 25, 2003
Last modified: July 6, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.