Reviewed, UniProtKB/Swiss-Prot Q8K2Q7 (BROX_MOUSE)

Names and origin

Protein names

Recommended name:
    BRO1 domain-containing protein BROX

Gene names

Name:

Brox

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Subunit structure	INTERACTS WITH CHMP4B By similarity.
Subcellular location	Membrane; Lipid-anchor Potential.
Sequence similarities	Belongs to the BROX family. Contains 1 BRO1 domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
PTM	Lipoprotein Prenylation
Gene Ontology (GO)
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8K2Q7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8K2Q7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     102-103: SA → RF
     104-411: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	BRO1 domain-containing protein BROX		PRO_0000304613
Regions
	Domain	90 – 411	322	BRO1
Amino acid modifications
	Lipidation	408	1	S-farnesyl cysteine By similarity
Natural variations
	Alternative sequence	102 – 103	2	SA → RF in isoform 2.		VSP_028041
	Alternative sequence	104 – 411	308	Missing in isoform 2.		VSP_028042
Experimental info
	Sequence conflict	163	1	P → S in BAC34307. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 5079195FBA23DF87	FASTA	411	46,202
	10 20 30 40 50 60 MTHWFHRNPL KATAPVSFNY YGMITGPPAS KICNDLRSAR TRLLELFTDL SCNPETMKNA 70 80 90 100 110 120 ADLYFSLLQG FINSVGDSTQ ESKLRYIQNF KWTDTLQGHV PSAQQDAVFE LISMGFNVAL 130 140 150 160 170 180 WYTKYASRLA GKENITEDEA KEVHRSLKIA AGIFKHLKES HIPKLLTPAE KGRDLEARLI 190 200 210 220 230 240 DAYIIQCQAE AQEVTIARAI ELKHAPGLIA ALAYDTASFY QKADHTLSSL EPAHSAKWRK 250 260 270 280 290 300 YLHLKMCFYT AYAYCYHGQT LLASDKCGEA IRSLQEAEKL YAEAEALCKE YGETKGPGPT 310 320 330 340 350 360 AKPSGHLFFR KLGSLVKNTL DKCQRENGFI YFQKIPTEAP QLELKANYGL VEPVPFEFPP 370 380 390 400 410 MSAHWTPEAL AAFDLTKRPK DDSVKPKPEE DVKPVKEPDI RPQKDTGCSV S
	Isoform 2. Checksum: 8AD95D0E8FD5F7F5	FASTA	103	11,832
	10 20 30 40 50 60 MTHWFHRNPL KATAPVSFNY YGMITGPPAS KICNDLRSAR TRLLELFTDL SCNPETMKNA 70 80 90 100 ADLYFSLLQG FINSVGDSTQ ESKLRYIQNF KWTDTLQGHV PRF

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Egg, Kidney and Pancreas.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK002489 mRNA. Translation: BAB22138.1. AK050525 mRNA. Translation: BAC34307.1. AK162247 mRNA. Translation: BAE36814.1. BC030340 mRNA. Translation: AAH30340.1. BC120692 mRNA. Translation: AAI20693.1. BC125527 mRNA. Translation: AAI25528.1.
IPI	IPI00453679. IPI00857687.
RefSeq	NP_082137.1.
UniGene	Mm.392270
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q8K2Q7.
Proteomic databases
PRIDE	Q8K2Q7.
Genome annotation databases
Ensembl	ENSMUST00000057062; ENSMUSP00000061345; ENSMUSG00000046836; Mus musculus. [Genome view]
GeneID	71678.
KEGG	mmu:71678.
UCSC	uc008icq.1. mouse.
Organism-specific databases
MGI	MGI:1918928. 0610010K06Rik.
Phylogenomic databases
HOGENOM	HBG279654.
HOVERGEN	Q8K2Q7.
InParanoid	Q8K2Q7.
OMA	DTLQGQV.
OrthoDB	EOG9NP9NZ.
Gene expression databases
Bgee	Q8K2Q7.
CleanEx	MM_0610010K06RIK.
Genevestigator	Q8K2Q7.
Family and domain databases
InterPro	IPR004328. BRO1. [Graphical view]
Pfam	PF03097. BRO1. 1 hit. [Graphical view]
PROSITE	PS51180. BRO1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	334207.
SOURCE	Search...

Entry information

Entry name

BROX_MOUSE

Accession

Primary (citable) accession number: Q8K2Q7
Secondary accession number(s): Q8BWM8, Q9DCT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 1, 2002
Last modified:	December 15, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)