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Q8K2P7

- S38A1_MOUSE

UniProt

Q8K2P7 - S38A1_MOUSE

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Protein

Sodium-coupled neutral amino acid transporter 1

Gene

Slc38a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA.1 Publication

Enzyme regulationi

Inhibited by lithium, potassium, choline ions, N-methyl-D-glucamine and 2-methylamino-isobutyric acid (MeAIB).By similarity

Kineticsi

Decrease in pH from 8.0 to 7.0 results in decreased affinity for L-glutamine.

  1. KM=2400 µM for L-glutamine (at pH 7.0)1 Publication
  2. KM=890 µM for L-glutamine (at pH 7.5)1 Publication
  3. KM=540 µM for L-glutamine (at pH 8.0)1 Publication

GO - Molecular functioni

  1. L-glutamine transmembrane transporter activity Source: MGI
  2. symporter activity Source: UniProtKB-KW

GO - Biological processi

  1. glutamine transport Source: MGI
  2. sodium ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-coupled neutral amino acid transporter 1
Alternative name(s):
Amino acid transporter A1
MNat2
N-system amino acid transporter 2
Solute carrier family 38 member 1
System A amino acid transporter 1
System N amino acid transporter 1
Gene namesi
Name:Slc38a1
Synonyms:Nat2, Snat1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:2145895. Slc38a1.

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity
Note: Restricted to the somatodendritic compartment of neurons. Found in the cellular processes of neurons in the developing brain.By similarity

GO - Cellular componenti

  1. axon Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane Source: MGI
  4. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485Sodium-coupled neutral amino acid transporter 1PRO_0000310476Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei25 – 251PhosphoserineBy similarity
Modified residuei28 – 281PhosphoserineBy similarity
Modified residuei52 – 521PhosphoserineBy similarity
Modified residuei54 – 541PhosphothreonineBy similarity
Modified residuei56 – 561PhosphoserineBy similarity
Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi257 – 2571N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8K2P7.
PRIDEiQ8K2P7.

PTM databases

PhosphoSiteiQ8K2P7.

Expressioni

Tissue specificityi

Specifically expressed in brain and retina (at protein level). Also detected in spleen, small intestine and lung.1 Publication

Inductioni

Up-regulated by BDNF.1 Publication

Gene expression databases

BgeeiQ8K2P7.
CleanExiMM_SLC38A1.
GenevestigatoriQ8K2P7.

Structurei

3D structure databases

ProteinModelPortaliQ8K2P7.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7474CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini98 – 11215ExtracellularSequence AnalysisAdd
BLAST
Topological domaini134 – 14815CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini170 – 18819ExtracellularSequence AnalysisAdd
BLAST
Topological domaini212 – 2165CytoplasmicSequence Analysis
Topological domaini238 – 27336ExtracellularSequence AnalysisAdd
BLAST
Topological domaini295 – 31016CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini332 – 34817ExtracellularSequence AnalysisAdd
BLAST
Topological domaini370 – 39122CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini413 – 4142ExtracellularSequence Analysis
Topological domaini436 – 45015CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini472 – 48514ExtracellularSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei75 – 9723HelicalSequence AnalysisAdd
BLAST
Transmembranei113 – 13321HelicalSequence AnalysisAdd
BLAST
Transmembranei149 – 16921HelicalSequence AnalysisAdd
BLAST
Transmembranei189 – 21123HelicalSequence AnalysisAdd
BLAST
Transmembranei217 – 23721HelicalSequence AnalysisAdd
BLAST
Transmembranei274 – 29421HelicalSequence AnalysisAdd
BLAST
Transmembranei311 – 33121HelicalSequence AnalysisAdd
BLAST
Transmembranei349 – 36921HelicalSequence AnalysisAdd
BLAST
Transmembranei392 – 41221HelicalSequence AnalysisAdd
BLAST
Transmembranei415 – 43521HelicalSequence AnalysisAdd
BLAST
Transmembranei451 – 47121HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0814.
GeneTreeiENSGT00760000119147.
HOVERGENiHBG059571.
InParanoidiQ8K2P7.
KOiK14990.
OMAiIPCIVSE.
OrthoDBiEOG7WHH9J.
PhylomeDBiQ8K2P7.
TreeFamiTF328787.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8K2P7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMHFKSGLEL TELQNMTVPE DDNVSNDSND FTEVENGQIN SKFISDRESR
60 70 80 90 100
RSLTNSHLEK RKCDEYIPGT TSLGMSVFNL SNAIMGSGIL GLAFALANTG
110 120 130 140 150
ILLFLILLTS VTLLSIYSIN LLLICSKETG CMVYEKLGEQ VFGTTGKLVI
160 170 180 190 200
FGATSLQNTG AMLSYLFIVK NELPSAIKSL MGEEDAFSAW YVDGRVLVVM
210 220 230 240 250
VTFGIILPLC LLKNLGYLGY TSGFSLSCMM FFLIVVIYKK FQTPCMSVEQ
260 270 280 290 300
NSTVSANVTD ACTPKYVTFN SKTVYALPTI AFAFVCHPSV LPIYSELKDR
310 320 330 340 350
SQKKMQMVSN ISFFAMFVMY FLTAIFGYLT FYEKVQSDLL HKYQSTGDIL
360 370 380 390 400
ILTVRLAVIV AVILTVPVLF FTVRSSLFEL AKKTKFHLCR HVLVTIILLI
410 420 430 440 450
IINLLVIFIP SMKDIFGVVG VTSANMLIFI LPSSLYLKIT NQDGDKGTQR
460 470 480
IWAALFLGLG VLFSLISIPL VIYDWACSSG TDEGH
Length:485
Mass (Da):53,795
Last modified:October 1, 2002 - v1
Checksum:i1171E1458818977C
GO
Isoform 2 (identifier: Q8K2P7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     333-344: EKVQSDLLHKYQ → GKLLPLAMPICT
     345-485: Missing.

Note: No experimental confirmation available.

Show »
Length:344
Mass (Da):38,112
Checksum:i1AFA524A4E9C22AE
GO
Isoform 3 (identifier: Q8K2P7-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     67-72: IPGTTS → VSIICM
     73-485: Missing.

Show »
Length:72
Mass (Da):8,358
Checksum:i01A6F7B92D9D8741
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti48 – 481E → G in BAE38067. (PubMed:16141072)Curated
Sequence conflicti244 – 2441P → H in BAC33064. (PubMed:16141072)Curated
Sequence conflicti354 – 3541V → A in AAG43433. (PubMed:11325958)Curated
Sequence conflicti360 – 3601V → A in AAG43433. (PubMed:11325958)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei67 – 726IPGTTS → VSIICM in isoform 3. 1 PublicationVSP_029305
Alternative sequencei73 – 485413Missing in isoform 3. 1 PublicationVSP_029306Add
BLAST
Alternative sequencei333 – 34412EKVQS…LHKYQ → GKLLPLAMPICT in isoform 2. 1 PublicationVSP_029307Add
BLAST
Alternative sequencei345 – 485141Missing in isoform 2. 1 PublicationVSP_029308Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF184240 mRNA. Translation: AAG43433.2.
AK029189 mRNA. Translation: BAC26341.1.
AK034130 mRNA. Translation: BAC28597.1.
AK035098 mRNA. Translation: BAC28944.1.
AK047459 mRNA. Translation: BAC33064.1.
AK049294 mRNA. Translation: BAC33663.1.
AK050914 mRNA. Translation: BAC34457.1.
AK081724 mRNA. Translation: BAC38310.1.
AK162612 mRNA. Translation: BAE36989.1.
AK163914 mRNA. Translation: BAE37532.1.
AK165188 mRNA. Translation: BAE38067.1.
BC030378 mRNA. Translation: AAH30378.1.
BC066815 mRNA. Translation: AAH66815.1.
CCDSiCCDS27777.1. [Q8K2P7-1]
RefSeqiNP_001159928.1. NM_001166456.1. [Q8K2P7-1]
NP_001159930.1. NM_001166458.1. [Q8K2P7-1]
NP_598847.2. NM_134086.4. [Q8K2P7-1]
XP_006520298.1. XM_006520235.1. [Q8K2P7-1]
UniGeneiMm.103568.

Genome annotation databases

EnsembliENSMUST00000088452; ENSMUSP00000085799; ENSMUSG00000023169. [Q8K2P7-1]
ENSMUST00000088454; ENSMUSP00000085801; ENSMUSG00000023169. [Q8K2P7-1]
ENSMUST00000100262; ENSMUSP00000097833; ENSMUSG00000023169. [Q8K2P7-1]
GeneIDi105727.
KEGGimmu:105727.
UCSCiuc007xkh.2. mouse. [Q8K2P7-1]
uc007xkk.2. mouse. [Q8K2P7-2]
uc007xkl.2. mouse. [Q8K2P7-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF184240 mRNA. Translation: AAG43433.2 .
AK029189 mRNA. Translation: BAC26341.1 .
AK034130 mRNA. Translation: BAC28597.1 .
AK035098 mRNA. Translation: BAC28944.1 .
AK047459 mRNA. Translation: BAC33064.1 .
AK049294 mRNA. Translation: BAC33663.1 .
AK050914 mRNA. Translation: BAC34457.1 .
AK081724 mRNA. Translation: BAC38310.1 .
AK162612 mRNA. Translation: BAE36989.1 .
AK163914 mRNA. Translation: BAE37532.1 .
AK165188 mRNA. Translation: BAE38067.1 .
BC030378 mRNA. Translation: AAH30378.1 .
BC066815 mRNA. Translation: AAH66815.1 .
CCDSi CCDS27777.1. [Q8K2P7-1 ]
RefSeqi NP_001159928.1. NM_001166456.1. [Q8K2P7-1 ]
NP_001159930.1. NM_001166458.1. [Q8K2P7-1 ]
NP_598847.2. NM_134086.4. [Q8K2P7-1 ]
XP_006520298.1. XM_006520235.1. [Q8K2P7-1 ]
UniGenei Mm.103568.

3D structure databases

ProteinModelPortali Q8K2P7.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q8K2P7.

Proteomic databases

MaxQBi Q8K2P7.
PRIDEi Q8K2P7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000088452 ; ENSMUSP00000085799 ; ENSMUSG00000023169 . [Q8K2P7-1 ]
ENSMUST00000088454 ; ENSMUSP00000085801 ; ENSMUSG00000023169 . [Q8K2P7-1 ]
ENSMUST00000100262 ; ENSMUSP00000097833 ; ENSMUSG00000023169 . [Q8K2P7-1 ]
GeneIDi 105727.
KEGGi mmu:105727.
UCSCi uc007xkh.2. mouse. [Q8K2P7-1 ]
uc007xkk.2. mouse. [Q8K2P7-2 ]
uc007xkl.2. mouse. [Q8K2P7-3 ]

Organism-specific databases

CTDi 81539.
MGIi MGI:2145895. Slc38a1.

Phylogenomic databases

eggNOGi COG0814.
GeneTreei ENSGT00760000119147.
HOVERGENi HBG059571.
InParanoidi Q8K2P7.
KOi K14990.
OMAi IPCIVSE.
OrthoDBi EOG7WHH9J.
PhylomeDBi Q8K2P7.
TreeFami TF328787.

Miscellaneous databases

NextBioi 357848.
PROi Q8K2P7.
SOURCEi Search...

Gene expression databases

Bgeei Q8K2P7.
CleanExi MM_SLC38A1.
Genevestigatori Q8K2P7.

Family and domain databases

InterProi IPR013057. AA_transpt_TM.
[Graphical view ]
Pfami PF01490. Aa_trans. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of an N-system amino acid transporter expressed in retina and its involvement in glutamine transport."
    Gu S., Roderick H.L., Camacho P., Jiang J.X.
    J. Biol. Chem. 276:24137-24144(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Cerebellum, Diencephalon, Embryo, Embryonic head, Embryonic stem cell and Head.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: CD-1 and FVB/N-3.
    Tissue: Mammary tumor and Neural stem cell.
  4. "A critical role for system A amino acid transport in the regulation of dendritic development by brain-derived neurotrophic factor (BDNF)."
    Burkhalter J., Fiumelli H., Erickson J.D., Martin J.-L.
    J. Biol. Chem. 282:5152-5159(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY BDNF.

Entry informationi

Entry nameiS38A1_MOUSE
AccessioniPrimary (citable) accession number: Q8K2P7
Secondary accession number(s): Q3TNM1
, Q3TQ53, Q6NXZ2, Q8BHI3, Q8BXE2, Q99PR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3