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Q8K2P7 (S38A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium-coupled neutral amino acid transporter 1
Alternative name(s):
Amino acid transporter A1
MNat2
N-system amino acid transporter 2
Solute carrier family 38 member 1
System A amino acid transporter 1
System N amino acid transporter 1
Gene names
Name:Slc38a1
Synonyms:Nat2, Snat1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length485 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA. Ref.1

Enzyme regulation

Inhibited by lithium, potassium, choline ions, N-methyl-D-glucamine and 2-methylamino-isobutyric acid (MeAIB) By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Note: Restricted to the somatodendritic compartment of neurons By similarity. Found in the cellular processes of neurons in the developing brain By similarity.

Tissue specificity

Specifically expressed in brain and retina (at protein level). Also detected in spleen, small intestine and lung. Ref.1

Induction

Up-regulated by BDNF. Ref.4

Sequence similarities

Belongs to the amino acid/polyamine transporter 2 family.

Biophysicochemical properties

Kinetic parameters:

Decrease in pH from 8.0 to 7.0 results in decreased affinity for L-glutamine.

KM=2400 µM for L-glutamine (at pH 7.0) Ref.1

KM=890 µM for L-glutamine (at pH 7.5)

KM=540 µM for L-glutamine (at pH 8.0)

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8K2P7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8K2P7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     333-344: EKVQSDLLHKYQ → GKLLPLAMPICT
     345-485: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q8K2P7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     67-72: IPGTTS → VSIICM
     73-485: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 485485Sodium-coupled neutral amino acid transporter 1
PRO_0000310476

Regions

Topological domain1 – 7474Cytoplasmic Potential
Transmembrane75 – 9723Helical; Potential
Topological domain98 – 11215Extracellular Potential
Transmembrane113 – 13321Helical; Potential
Topological domain134 – 14815Cytoplasmic Potential
Transmembrane149 – 16921Helical; Potential
Topological domain170 – 18819Extracellular Potential
Transmembrane189 – 21123Helical; Potential
Topological domain212 – 2165Cytoplasmic Potential
Transmembrane217 – 23721Helical; Potential
Topological domain238 – 27336Extracellular Potential
Transmembrane274 – 29421Helical; Potential
Topological domain295 – 31016Cytoplasmic Potential
Transmembrane311 – 33121Helical; Potential
Topological domain332 – 34817Extracellular Potential
Transmembrane349 – 36921Helical; Potential
Topological domain370 – 39122Cytoplasmic Potential
Transmembrane392 – 41221Helical; Potential
Topological domain413 – 4142Extracellular Potential
Transmembrane415 – 43521Helical; Potential
Topological domain436 – 45015Cytoplasmic Potential
Transmembrane451 – 47121Helical; Potential
Topological domain472 – 48514Extracellular Potential

Amino acid modifications

Modified residue251Phosphoserine By similarity
Modified residue281Phosphoserine By similarity
Modified residue521Phosphoserine By similarity
Modified residue541Phosphothreonine By similarity
Modified residue561Phosphoserine By similarity
Glycosylation2511N-linked (GlcNAc...) Potential
Glycosylation2571N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence67 – 726IPGTTS → VSIICM in isoform 3.
VSP_029305
Alternative sequence73 – 485413Missing in isoform 3.
VSP_029306
Alternative sequence333 – 34412EKVQS…LHKYQ → GKLLPLAMPICT in isoform 2.
VSP_029307
Alternative sequence345 – 485141Missing in isoform 2.
VSP_029308

Experimental info

Sequence conflict481E → G in BAE38067. Ref.2
Sequence conflict2441P → H in BAC33064. Ref.2
Sequence conflict3541V → A in AAG43433. Ref.1
Sequence conflict3601V → A in AAG43433. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 1171E1458818977C

FASTA48553,795
        10         20         30         40         50         60 
MMHFKSGLEL TELQNMTVPE DDNVSNDSND FTEVENGQIN SKFISDRESR RSLTNSHLEK 

        70         80         90        100        110        120 
RKCDEYIPGT TSLGMSVFNL SNAIMGSGIL GLAFALANTG ILLFLILLTS VTLLSIYSIN 

       130        140        150        160        170        180 
LLLICSKETG CMVYEKLGEQ VFGTTGKLVI FGATSLQNTG AMLSYLFIVK NELPSAIKSL 

       190        200        210        220        230        240 
MGEEDAFSAW YVDGRVLVVM VTFGIILPLC LLKNLGYLGY TSGFSLSCMM FFLIVVIYKK 

       250        260        270        280        290        300 
FQTPCMSVEQ NSTVSANVTD ACTPKYVTFN SKTVYALPTI AFAFVCHPSV LPIYSELKDR 

       310        320        330        340        350        360 
SQKKMQMVSN ISFFAMFVMY FLTAIFGYLT FYEKVQSDLL HKYQSTGDIL ILTVRLAVIV 

       370        380        390        400        410        420 
AVILTVPVLF FTVRSSLFEL AKKTKFHLCR HVLVTIILLI IINLLVIFIP SMKDIFGVVG 

       430        440        450        460        470        480 
VTSANMLIFI LPSSLYLKIT NQDGDKGTQR IWAALFLGLG VLFSLISIPL VIYDWACSSG 


TDEGH 

« Hide

Isoform 2 [UniParc].

Checksum: 1AFA524A4E9C22AE
Show »

FASTA34438,112
Isoform 3 [UniParc].

Checksum: 01A6F7B92D9D8741
Show »

FASTA728,358

References

« Hide 'large scale' references
[1]"Characterization of an N-system amino acid transporter expressed in retina and its involvement in glutamine transport."
Gu S., Roderick H.L., Camacho P., Jiang J.X.
J. Biol. Chem. 276:24137-24144(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Cerebellum, Diencephalon, Embryo, Embryonic head, Embryonic stem cell and Head.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Strain: CD-1 and FVB/N-3.
Tissue: Mammary tumor and Neural stem cell.
[4]"A critical role for system A amino acid transport in the regulation of dendritic development by brain-derived neurotrophic factor (BDNF)."
Burkhalter J., Fiumelli H., Erickson J.D., Martin J.-L.
J. Biol. Chem. 282:5152-5159(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY BDNF.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF184240 mRNA. Translation: AAG43433.2.
AK029189 mRNA. Translation: BAC26341.1.
AK034130 mRNA. Translation: BAC28597.1.
AK035098 mRNA. Translation: BAC28944.1.
AK047459 mRNA. Translation: BAC33064.1.
AK049294 mRNA. Translation: BAC33663.1.
AK050914 mRNA. Translation: BAC34457.1.
AK081724 mRNA. Translation: BAC38310.1.
AK162612 mRNA. Translation: BAE36989.1.
AK163914 mRNA. Translation: BAE37532.1.
AK165188 mRNA. Translation: BAE38067.1.
BC030378 mRNA. Translation: AAH30378.1.
BC066815 mRNA. Translation: AAH66815.1.
CCDSCCDS27777.1. [Q8K2P7-1]
RefSeqNP_001159928.1. NM_001166456.1. [Q8K2P7-1]
NP_001159930.1. NM_001166458.1. [Q8K2P7-1]
NP_598847.2. NM_134086.4. [Q8K2P7-1]
XP_006520298.1. XM_006520235.1. [Q8K2P7-1]
UniGeneMm.103568.

3D structure databases

ProteinModelPortalQ8K2P7.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8K2P7.

Proteomic databases

PRIDEQ8K2P7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000088452; ENSMUSP00000085799; ENSMUSG00000023169. [Q8K2P7-1]
ENSMUST00000088454; ENSMUSP00000085801; ENSMUSG00000023169. [Q8K2P7-1]
ENSMUST00000100262; ENSMUSP00000097833; ENSMUSG00000023169. [Q8K2P7-1]
GeneID105727.
KEGGmmu:105727.
UCSCuc007xkh.2. mouse. [Q8K2P7-1]
uc007xkk.2. mouse. [Q8K2P7-2]
uc007xkl.2. mouse. [Q8K2P7-3]

Organism-specific databases

CTD81539.
MGIMGI:2145895. Slc38a1.

Phylogenomic databases

eggNOGCOG0814.
GeneTreeENSGT00710000106531.
HOVERGENHBG059571.
InParanoidQ8K2P7.
KOK14990.
OMAIPCIVSE.
OrthoDBEOG7WHH9J.
PhylomeDBQ8K2P7.
TreeFamTF328787.

Gene expression databases

BgeeQ8K2P7.
CleanExMM_SLC38A1.
GenevestigatorQ8K2P7.

Family and domain databases

InterProIPR013057. AA_transpt_TM.
[Graphical view]
PfamPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio357848.
PROQ8K2P7.
SOURCESearch...

Entry information

Entry nameS38A1_MOUSE
AccessionPrimary (citable) accession number: Q8K2P7
Secondary accession number(s): Q3TNM1 expand/collapse secondary AC list , Q3TQ53, Q6NXZ2, Q8BHI3, Q8BXE2, Q99PR1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot