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Protein

Sodium-coupled neutral amino acid transporter 1

Gene

Slc38a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA.1 Publication

Enzyme regulationi

Inhibited by lithium, potassium, choline ions, N-methyl-D-glucamine and 2-methylamino-isobutyric acid (MeAIB).By similarity

Kineticsi

Decrease in pH from 8.0 to 7.0 results in decreased affinity for L-glutamine.

  1. KM=2400 µM for L-glutamine (at pH 7.0)1 Publication
  2. KM=890 µM for L-glutamine (at pH 7.5)1 Publication
  3. KM=540 µM for L-glutamine (at pH 8.0)1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Biological processi

    Amino-acid transport, Ion transport, Sodium transport, Symport, Transport

    Keywords - Ligandi

    Sodium

    Enzyme and pathway databases

    ReactomeiR-MMU-210455. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
    R-MMU-352230. Amino acid transport across the plasma membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium-coupled neutral amino acid transporter 1
    Alternative name(s):
    Amino acid transporter A1
    MNat2
    N-system amino acid transporter 2
    Solute carrier family 38 member 1
    System A amino acid transporter 1
    System N amino acid transporter 1
    Gene namesi
    Name:Slc38a1
    Synonyms:Nat2, Snat1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 15

    Organism-specific databases

    MGIiMGI:2145895. Slc38a1.

    Subcellular locationi

    • Cell membrane By similarity; Multi-pass membrane protein By similarity

    • Note: Restricted to the somatodendritic compartment of neurons. Found in the cellular processes of neurons in the developing brain.By similarity

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 74CytoplasmicSequence analysisAdd BLAST74
    Transmembranei75 – 97HelicalSequence analysisAdd BLAST23
    Topological domaini98 – 112ExtracellularSequence analysisAdd BLAST15
    Transmembranei113 – 133HelicalSequence analysisAdd BLAST21
    Topological domaini134 – 148CytoplasmicSequence analysisAdd BLAST15
    Transmembranei149 – 169HelicalSequence analysisAdd BLAST21
    Topological domaini170 – 188ExtracellularSequence analysisAdd BLAST19
    Transmembranei189 – 211HelicalSequence analysisAdd BLAST23
    Topological domaini212 – 216CytoplasmicSequence analysis5
    Transmembranei217 – 237HelicalSequence analysisAdd BLAST21
    Topological domaini238 – 273ExtracellularSequence analysisAdd BLAST36
    Transmembranei274 – 294HelicalSequence analysisAdd BLAST21
    Topological domaini295 – 310CytoplasmicSequence analysisAdd BLAST16
    Transmembranei311 – 331HelicalSequence analysisAdd BLAST21
    Topological domaini332 – 348ExtracellularSequence analysisAdd BLAST17
    Transmembranei349 – 369HelicalSequence analysisAdd BLAST21
    Topological domaini370 – 391CytoplasmicSequence analysisAdd BLAST22
    Transmembranei392 – 412HelicalSequence analysisAdd BLAST21
    Topological domaini413 – 414ExtracellularSequence analysis2
    Transmembranei415 – 435HelicalSequence analysisAdd BLAST21
    Topological domaini436 – 450CytoplasmicSequence analysisAdd BLAST15
    Transmembranei451 – 471HelicalSequence analysisAdd BLAST21
    Topological domaini472 – 485ExtracellularSequence analysisAdd BLAST14

    GO - Cellular componenti

    • axon Source: MGI
    • extracellular exosome Source: MGI
    • integral component of plasma membrane Source: GO_Central
    • membrane Source: MGI
    • plasma membrane Source: MGI
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003104761 – 485Sodium-coupled neutral amino acid transporter 1Add BLAST485

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei6PhosphoserineCombined sources1
    Modified residuei11PhosphothreonineCombined sources1
    Modified residuei25PhosphoserineBy similarity1
    Modified residuei28PhosphoserineBy similarity1
    Modified residuei49PhosphoserineBy similarity1
    Modified residuei52PhosphoserineBy similarity1
    Modified residuei54PhosphothreonineBy similarity1
    Modified residuei56PhosphoserineBy similarity1
    Glycosylationi251N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi257N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ8K2P7.
    PeptideAtlasiQ8K2P7.
    PRIDEiQ8K2P7.

    PTM databases

    iPTMnetiQ8K2P7.
    PhosphoSitePlusiQ8K2P7.

    Expressioni

    Tissue specificityi

    Specifically expressed in brain and retina (at protein level). Also detected in spleen, small intestine and lung.1 Publication

    Inductioni

    Up-regulated by BDNF.1 Publication

    Gene expression databases

    BgeeiENSMUSG00000023169.
    CleanExiMM_SLC38A1.
    GenevisibleiQ8K2P7. MM.

    Interactioni

    Protein-protein interaction databases

    BioGridi222905. 1 interactor.
    STRINGi10090.ENSMUSP00000085799.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K2P7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1305. Eukaryota.
    COG0814. LUCA.
    GeneTreeiENSGT00760000119147.
    HOVERGENiHBG059571.
    InParanoidiQ8K2P7.
    KOiK14990.
    OMAiQILCLES.
    OrthoDBiEOG091G0GP8.
    PhylomeDBiQ8K2P7.
    TreeFamiTF328787.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q8K2P7-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MMHFKSGLEL TELQNMTVPE DDNVSNDSND FTEVENGQIN SKFISDRESR
    60 70 80 90 100
    RSLTNSHLEK RKCDEYIPGT TSLGMSVFNL SNAIMGSGIL GLAFALANTG
    110 120 130 140 150
    ILLFLILLTS VTLLSIYSIN LLLICSKETG CMVYEKLGEQ VFGTTGKLVI
    160 170 180 190 200
    FGATSLQNTG AMLSYLFIVK NELPSAIKSL MGEEDAFSAW YVDGRVLVVM
    210 220 230 240 250
    VTFGIILPLC LLKNLGYLGY TSGFSLSCMM FFLIVVIYKK FQTPCMSVEQ
    260 270 280 290 300
    NSTVSANVTD ACTPKYVTFN SKTVYALPTI AFAFVCHPSV LPIYSELKDR
    310 320 330 340 350
    SQKKMQMVSN ISFFAMFVMY FLTAIFGYLT FYEKVQSDLL HKYQSTGDIL
    360 370 380 390 400
    ILTVRLAVIV AVILTVPVLF FTVRSSLFEL AKKTKFHLCR HVLVTIILLI
    410 420 430 440 450
    IINLLVIFIP SMKDIFGVVG VTSANMLIFI LPSSLYLKIT NQDGDKGTQR
    460 470 480
    IWAALFLGLG VLFSLISIPL VIYDWACSSG TDEGH
    Length:485
    Mass (Da):53,795
    Last modified:October 1, 2002 - v1
    Checksum:i1171E1458818977C
    GO
    Isoform 2 (identifier: Q8K2P7-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         333-344: EKVQSDLLHKYQ → GKLLPLAMPICT
         345-485: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:344
    Mass (Da):38,112
    Checksum:i1AFA524A4E9C22AE
    GO
    Isoform 3 (identifier: Q8K2P7-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         67-72: IPGTTS → VSIICM
         73-485: Missing.

    Show »
    Length:72
    Mass (Da):8,358
    Checksum:i01A6F7B92D9D8741
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti48E → G in BAE38067 (PubMed:16141072).Curated1
    Sequence conflicti244P → H in BAC33064 (PubMed:16141072).Curated1
    Sequence conflicti354V → A in AAG43433 (PubMed:11325958).Curated1
    Sequence conflicti360V → A in AAG43433 (PubMed:11325958).Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_02930567 – 72IPGTTS → VSIICM in isoform 3. 1 Publication6
    Alternative sequenceiVSP_02930673 – 485Missing in isoform 3. 1 PublicationAdd BLAST413
    Alternative sequenceiVSP_029307333 – 344EKVQS…LHKYQ → GKLLPLAMPICT in isoform 2. 1 PublicationAdd BLAST12
    Alternative sequenceiVSP_029308345 – 485Missing in isoform 2. 1 PublicationAdd BLAST141

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF184240 mRNA. Translation: AAG43433.2.
    AK029189 mRNA. Translation: BAC26341.1.
    AK034130 mRNA. Translation: BAC28597.1.
    AK035098 mRNA. Translation: BAC28944.1.
    AK047459 mRNA. Translation: BAC33064.1.
    AK049294 mRNA. Translation: BAC33663.1.
    AK050914 mRNA. Translation: BAC34457.1.
    AK081724 mRNA. Translation: BAC38310.1.
    AK162612 mRNA. Translation: BAE36989.1.
    AK163914 mRNA. Translation: BAE37532.1.
    AK165188 mRNA. Translation: BAE38067.1.
    BC030378 mRNA. Translation: AAH30378.1.
    BC066815 mRNA. Translation: AAH66815.1.
    CCDSiCCDS27777.1. [Q8K2P7-1]
    RefSeqiNP_001159928.1. NM_001166456.1. [Q8K2P7-1]
    NP_001159930.1. NM_001166458.1. [Q8K2P7-1]
    NP_598847.2. NM_134086.4. [Q8K2P7-1]
    XP_006520298.1. XM_006520235.3. [Q8K2P7-1]
    UniGeneiMm.103568.

    Genome annotation databases

    EnsembliENSMUST00000088452; ENSMUSP00000085799; ENSMUSG00000023169. [Q8K2P7-1]
    ENSMUST00000088454; ENSMUSP00000085801; ENSMUSG00000023169. [Q8K2P7-1]
    ENSMUST00000100262; ENSMUSP00000097833; ENSMUSG00000023169. [Q8K2P7-1]
    GeneIDi105727.
    KEGGimmu:105727.
    UCSCiuc007xkh.2. mouse. [Q8K2P7-1]
    uc007xkk.2. mouse. [Q8K2P7-2]
    uc007xkl.2. mouse. [Q8K2P7-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF184240 mRNA. Translation: AAG43433.2.
    AK029189 mRNA. Translation: BAC26341.1.
    AK034130 mRNA. Translation: BAC28597.1.
    AK035098 mRNA. Translation: BAC28944.1.
    AK047459 mRNA. Translation: BAC33064.1.
    AK049294 mRNA. Translation: BAC33663.1.
    AK050914 mRNA. Translation: BAC34457.1.
    AK081724 mRNA. Translation: BAC38310.1.
    AK162612 mRNA. Translation: BAE36989.1.
    AK163914 mRNA. Translation: BAE37532.1.
    AK165188 mRNA. Translation: BAE38067.1.
    BC030378 mRNA. Translation: AAH30378.1.
    BC066815 mRNA. Translation: AAH66815.1.
    CCDSiCCDS27777.1. [Q8K2P7-1]
    RefSeqiNP_001159928.1. NM_001166456.1. [Q8K2P7-1]
    NP_001159930.1. NM_001166458.1. [Q8K2P7-1]
    NP_598847.2. NM_134086.4. [Q8K2P7-1]
    XP_006520298.1. XM_006520235.3. [Q8K2P7-1]
    UniGeneiMm.103568.

    3D structure databases

    ProteinModelPortaliQ8K2P7.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi222905. 1 interactor.
    STRINGi10090.ENSMUSP00000085799.

    PTM databases

    iPTMnetiQ8K2P7.
    PhosphoSitePlusiQ8K2P7.

    Proteomic databases

    PaxDbiQ8K2P7.
    PeptideAtlasiQ8K2P7.
    PRIDEiQ8K2P7.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000088452; ENSMUSP00000085799; ENSMUSG00000023169. [Q8K2P7-1]
    ENSMUST00000088454; ENSMUSP00000085801; ENSMUSG00000023169. [Q8K2P7-1]
    ENSMUST00000100262; ENSMUSP00000097833; ENSMUSG00000023169. [Q8K2P7-1]
    GeneIDi105727.
    KEGGimmu:105727.
    UCSCiuc007xkh.2. mouse. [Q8K2P7-1]
    uc007xkk.2. mouse. [Q8K2P7-2]
    uc007xkl.2. mouse. [Q8K2P7-3]

    Organism-specific databases

    CTDi81539.
    MGIiMGI:2145895. Slc38a1.

    Phylogenomic databases

    eggNOGiKOG1305. Eukaryota.
    COG0814. LUCA.
    GeneTreeiENSGT00760000119147.
    HOVERGENiHBG059571.
    InParanoidiQ8K2P7.
    KOiK14990.
    OMAiQILCLES.
    OrthoDBiEOG091G0GP8.
    PhylomeDBiQ8K2P7.
    TreeFamiTF328787.

    Enzyme and pathway databases

    ReactomeiR-MMU-210455. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
    R-MMU-352230. Amino acid transport across the plasma membrane.

    Miscellaneous databases

    ChiTaRSiSlc38a1. mouse.
    PROiQ8K2P7.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000023169.
    CleanExiMM_SLC38A1.
    GenevisibleiQ8K2P7. MM.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiS38A1_MOUSE
    AccessioniPrimary (citable) accession number: Q8K2P7
    Secondary accession number(s): Q3TNM1
    , Q3TQ53, Q6NXZ2, Q8BHI3, Q8BXE2, Q99PR1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: October 1, 2002
    Last modified: November 2, 2016
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.