SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8K2P7

- S38A1_MOUSE

UniProt

Q8K2P7 - S38A1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Sodium-coupled neutral amino acid transporter 1

Gene
Slc38a1, Nat2, Snat1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA.1 Publication

Enzyme regulationi

Inhibited by lithium, potassium, choline ions, N-methyl-D-glucamine and 2-methylamino-isobutyric acid (MeAIB) By similarity.

Kineticsi

Decrease in pH from 8.0 to 7.0 results in decreased affinity for L-glutamine.

  1. KM=2400 µM for L-glutamine (at pH 7.0)1 Publication
  2. KM=890 µM for L-glutamine (at pH 7.5)
  3. KM=540 µM for L-glutamine (at pH 8.0)

GO - Molecular functioni

  1. L-glutamine transmembrane transporter activity Source: MGI
  2. symporter activity Source: UniProtKB-KW

GO - Biological processi

  1. glutamine transport Source: MGI
  2. sodium ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-coupled neutral amino acid transporter 1
Alternative name(s):
Amino acid transporter A1
MNat2
N-system amino acid transporter 2
Solute carrier family 38 member 1
System A amino acid transporter 1
System N amino acid transporter 1
Gene namesi
Name:Slc38a1
Synonyms:Nat2, Snat1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:2145895. Slc38a1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein By similarity
Note: Restricted to the somatodendritic compartment of neurons By similarity. Found in the cellular processes of neurons in the developing brain By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7474Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei75 – 9723Helical; Reviewed predictionAdd
BLAST
Topological domaini98 – 11215Extracellular Reviewed predictionAdd
BLAST
Transmembranei113 – 13321Helical; Reviewed predictionAdd
BLAST
Topological domaini134 – 14815Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei149 – 16921Helical; Reviewed predictionAdd
BLAST
Topological domaini170 – 18819Extracellular Reviewed predictionAdd
BLAST
Transmembranei189 – 21123Helical; Reviewed predictionAdd
BLAST
Topological domaini212 – 2165Cytoplasmic Reviewed prediction
Transmembranei217 – 23721Helical; Reviewed predictionAdd
BLAST
Topological domaini238 – 27336Extracellular Reviewed predictionAdd
BLAST
Transmembranei274 – 29421Helical; Reviewed predictionAdd
BLAST
Topological domaini295 – 31016Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei311 – 33121Helical; Reviewed predictionAdd
BLAST
Topological domaini332 – 34817Extracellular Reviewed predictionAdd
BLAST
Transmembranei349 – 36921Helical; Reviewed predictionAdd
BLAST
Topological domaini370 – 39122Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei392 – 41221Helical; Reviewed predictionAdd
BLAST
Topological domaini413 – 4142Extracellular Reviewed prediction
Transmembranei415 – 43521Helical; Reviewed predictionAdd
BLAST
Topological domaini436 – 45015Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei451 – 47121Helical; Reviewed predictionAdd
BLAST
Topological domaini472 – 48514Extracellular Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. axon Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane Source: MGI
  4. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485Sodium-coupled neutral amino acid transporter 1PRO_0000310476Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei25 – 251Phosphoserine By similarity
Modified residuei28 – 281Phosphoserine By similarity
Modified residuei52 – 521Phosphoserine By similarity
Modified residuei54 – 541Phosphothreonine By similarity
Modified residuei56 – 561Phosphoserine By similarity
Glycosylationi251 – 2511N-linked (GlcNAc...) Reviewed prediction
Glycosylationi257 – 2571N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ8K2P7.

PTM databases

PhosphoSiteiQ8K2P7.

Expressioni

Tissue specificityi

Specifically expressed in brain and retina (at protein level). Also detected in spleen, small intestine and lung.1 Publication

Inductioni

Up-regulated by BDNF.1 Publication

Gene expression databases

BgeeiQ8K2P7.
CleanExiMM_SLC38A1.
GenevestigatoriQ8K2P7.

Structurei

3D structure databases

ProteinModelPortaliQ8K2P7.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0814.
GeneTreeiENSGT00710000106531.
HOVERGENiHBG059571.
InParanoidiQ8K2P7.
KOiK14990.
OMAiIPCIVSE.
OrthoDBiEOG7WHH9J.
PhylomeDBiQ8K2P7.
TreeFamiTF328787.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8K2P7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MMHFKSGLEL TELQNMTVPE DDNVSNDSND FTEVENGQIN SKFISDRESR    50
RSLTNSHLEK RKCDEYIPGT TSLGMSVFNL SNAIMGSGIL GLAFALANTG 100
ILLFLILLTS VTLLSIYSIN LLLICSKETG CMVYEKLGEQ VFGTTGKLVI 150
FGATSLQNTG AMLSYLFIVK NELPSAIKSL MGEEDAFSAW YVDGRVLVVM 200
VTFGIILPLC LLKNLGYLGY TSGFSLSCMM FFLIVVIYKK FQTPCMSVEQ 250
NSTVSANVTD ACTPKYVTFN SKTVYALPTI AFAFVCHPSV LPIYSELKDR 300
SQKKMQMVSN ISFFAMFVMY FLTAIFGYLT FYEKVQSDLL HKYQSTGDIL 350
ILTVRLAVIV AVILTVPVLF FTVRSSLFEL AKKTKFHLCR HVLVTIILLI 400
IINLLVIFIP SMKDIFGVVG VTSANMLIFI LPSSLYLKIT NQDGDKGTQR 450
IWAALFLGLG VLFSLISIPL VIYDWACSSG TDEGH 485
Length:485
Mass (Da):53,795
Last modified:October 1, 2002 - v1
Checksum:i1171E1458818977C
GO
Isoform 2 (identifier: Q8K2P7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     333-344: EKVQSDLLHKYQ → GKLLPLAMPICT
     345-485: Missing.

Note: No experimental confirmation available.

Show »
Length:344
Mass (Da):38,112
Checksum:i1AFA524A4E9C22AE
GO
Isoform 3 (identifier: Q8K2P7-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     67-72: IPGTTS → VSIICM
     73-485: Missing.

Show »
Length:72
Mass (Da):8,358
Checksum:i01A6F7B92D9D8741
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei67 – 726IPGTTS → VSIICM in isoform 3. VSP_029305
Alternative sequencei73 – 485413Missing in isoform 3. VSP_029306Add
BLAST
Alternative sequencei333 – 34412EKVQS…LHKYQ → GKLLPLAMPICT in isoform 2. VSP_029307Add
BLAST
Alternative sequencei345 – 485141Missing in isoform 2. VSP_029308Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti48 – 481E → G in BAE38067. 1 Publication
Sequence conflicti244 – 2441P → H in BAC33064. 1 Publication
Sequence conflicti354 – 3541V → A in AAG43433. 1 Publication
Sequence conflicti360 – 3601V → A in AAG43433. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF184240 mRNA. Translation: AAG43433.2.
AK029189 mRNA. Translation: BAC26341.1.
AK034130 mRNA. Translation: BAC28597.1.
AK035098 mRNA. Translation: BAC28944.1.
AK047459 mRNA. Translation: BAC33064.1.
AK049294 mRNA. Translation: BAC33663.1.
AK050914 mRNA. Translation: BAC34457.1.
AK081724 mRNA. Translation: BAC38310.1.
AK162612 mRNA. Translation: BAE36989.1.
AK163914 mRNA. Translation: BAE37532.1.
AK165188 mRNA. Translation: BAE38067.1.
BC030378 mRNA. Translation: AAH30378.1.
BC066815 mRNA. Translation: AAH66815.1.
CCDSiCCDS27777.1. [Q8K2P7-1]
RefSeqiNP_001159928.1. NM_001166456.1. [Q8K2P7-1]
NP_001159930.1. NM_001166458.1. [Q8K2P7-1]
NP_598847.2. NM_134086.4. [Q8K2P7-1]
XP_006520298.1. XM_006520235.1. [Q8K2P7-1]
UniGeneiMm.103568.

Genome annotation databases

EnsembliENSMUST00000088452; ENSMUSP00000085799; ENSMUSG00000023169. [Q8K2P7-1]
ENSMUST00000088454; ENSMUSP00000085801; ENSMUSG00000023169. [Q8K2P7-1]
ENSMUST00000100262; ENSMUSP00000097833; ENSMUSG00000023169. [Q8K2P7-1]
GeneIDi105727.
KEGGimmu:105727.
UCSCiuc007xkh.2. mouse. [Q8K2P7-1]
uc007xkk.2. mouse. [Q8K2P7-2]
uc007xkl.2. mouse. [Q8K2P7-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF184240 mRNA. Translation: AAG43433.2 .
AK029189 mRNA. Translation: BAC26341.1 .
AK034130 mRNA. Translation: BAC28597.1 .
AK035098 mRNA. Translation: BAC28944.1 .
AK047459 mRNA. Translation: BAC33064.1 .
AK049294 mRNA. Translation: BAC33663.1 .
AK050914 mRNA. Translation: BAC34457.1 .
AK081724 mRNA. Translation: BAC38310.1 .
AK162612 mRNA. Translation: BAE36989.1 .
AK163914 mRNA. Translation: BAE37532.1 .
AK165188 mRNA. Translation: BAE38067.1 .
BC030378 mRNA. Translation: AAH30378.1 .
BC066815 mRNA. Translation: AAH66815.1 .
CCDSi CCDS27777.1. [Q8K2P7-1 ]
RefSeqi NP_001159928.1. NM_001166456.1. [Q8K2P7-1 ]
NP_001159930.1. NM_001166458.1. [Q8K2P7-1 ]
NP_598847.2. NM_134086.4. [Q8K2P7-1 ]
XP_006520298.1. XM_006520235.1. [Q8K2P7-1 ]
UniGenei Mm.103568.

3D structure databases

ProteinModelPortali Q8K2P7.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q8K2P7.

Proteomic databases

PRIDEi Q8K2P7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000088452 ; ENSMUSP00000085799 ; ENSMUSG00000023169 . [Q8K2P7-1 ]
ENSMUST00000088454 ; ENSMUSP00000085801 ; ENSMUSG00000023169 . [Q8K2P7-1 ]
ENSMUST00000100262 ; ENSMUSP00000097833 ; ENSMUSG00000023169 . [Q8K2P7-1 ]
GeneIDi 105727.
KEGGi mmu:105727.
UCSCi uc007xkh.2. mouse. [Q8K2P7-1 ]
uc007xkk.2. mouse. [Q8K2P7-2 ]
uc007xkl.2. mouse. [Q8K2P7-3 ]

Organism-specific databases

CTDi 81539.
MGIi MGI:2145895. Slc38a1.

Phylogenomic databases

eggNOGi COG0814.
GeneTreei ENSGT00710000106531.
HOVERGENi HBG059571.
InParanoidi Q8K2P7.
KOi K14990.
OMAi IPCIVSE.
OrthoDBi EOG7WHH9J.
PhylomeDBi Q8K2P7.
TreeFami TF328787.

Miscellaneous databases

NextBioi 357848.
PROi Q8K2P7.
SOURCEi Search...

Gene expression databases

Bgeei Q8K2P7.
CleanExi MM_SLC38A1.
Genevestigatori Q8K2P7.

Family and domain databases

InterProi IPR013057. AA_transpt_TM.
[Graphical view ]
Pfami PF01490. Aa_trans. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of an N-system amino acid transporter expressed in retina and its involvement in glutamine transport."
    Gu S., Roderick H.L., Camacho P., Jiang J.X.
    J. Biol. Chem. 276:24137-24144(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Cerebellum, Diencephalon, Embryo, Embryonic head, Embryonic stem cell and Head.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: CD-1 and FVB/N-3.
    Tissue: Mammary tumor and Neural stem cell.
  4. "A critical role for system A amino acid transport in the regulation of dendritic development by brain-derived neurotrophic factor (BDNF)."
    Burkhalter J., Fiumelli H., Erickson J.D., Martin J.-L.
    J. Biol. Chem. 282:5152-5159(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY BDNF.

Entry informationi

Entry nameiS38A1_MOUSE
AccessioniPrimary (citable) accession number: Q8K2P7
Secondary accession number(s): Q3TNM1
, Q3TQ53, Q6NXZ2, Q8BHI3, Q8BXE2, Q99PR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi