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Q8K2P7

- S38A1_MOUSE

UniProt

Q8K2P7 - S38A1_MOUSE

Protein

Sodium-coupled neutral amino acid transporter 1

Gene

Slc38a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA.1 Publication

    Enzyme regulationi

    Inhibited by lithium, potassium, choline ions, N-methyl-D-glucamine and 2-methylamino-isobutyric acid (MeAIB).By similarity

    Kineticsi

    Decrease in pH from 8.0 to 7.0 results in decreased affinity for L-glutamine.

    1. KM=2400 µM for L-glutamine (at pH 7.0)1 Publication
    2. KM=890 µM for L-glutamine (at pH 7.5)1 Publication
    3. KM=540 µM for L-glutamine (at pH 8.0)1 Publication

    GO - Molecular functioni

    1. L-glutamine transmembrane transporter activity Source: MGI
    2. symporter activity Source: UniProtKB-KW

    GO - Biological processi

    1. glutamine transport Source: MGI
    2. sodium ion transport Source: UniProtKB-KW

    Keywords - Biological processi

    Amino-acid transport, Ion transport, Sodium transport, Symport, Transport

    Keywords - Ligandi

    Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium-coupled neutral amino acid transporter 1
    Alternative name(s):
    Amino acid transporter A1
    MNat2
    N-system amino acid transporter 2
    Solute carrier family 38 member 1
    System A amino acid transporter 1
    System N amino acid transporter 1
    Gene namesi
    Name:Slc38a1
    Synonyms:Nat2, Snat1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:2145895. Slc38a1.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity
    Note: Restricted to the somatodendritic compartment of neurons. Found in the cellular processes of neurons in the developing brain.By similarity

    GO - Cellular componenti

    1. axon Source: MGI
    2. integral component of membrane Source: UniProtKB-KW
    3. membrane Source: MGI
    4. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 485485Sodium-coupled neutral amino acid transporter 1PRO_0000310476Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei25 – 251PhosphoserineBy similarity
    Modified residuei28 – 281PhosphoserineBy similarity
    Modified residuei52 – 521PhosphoserineBy similarity
    Modified residuei54 – 541PhosphothreonineBy similarity
    Modified residuei56 – 561PhosphoserineBy similarity
    Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi257 – 2571N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiQ8K2P7.

    PTM databases

    PhosphoSiteiQ8K2P7.

    Expressioni

    Tissue specificityi

    Specifically expressed in brain and retina (at protein level). Also detected in spleen, small intestine and lung.1 Publication

    Inductioni

    Up-regulated by BDNF.1 Publication

    Gene expression databases

    BgeeiQ8K2P7.
    CleanExiMM_SLC38A1.
    GenevestigatoriQ8K2P7.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K2P7.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 7474CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini98 – 11215ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini134 – 14815CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini170 – 18819ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini212 – 2165CytoplasmicSequence Analysis
    Topological domaini238 – 27336ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini295 – 31016CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini332 – 34817ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini370 – 39122CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini413 – 4142ExtracellularSequence Analysis
    Topological domaini436 – 45015CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini472 – 48514ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei75 – 9723HelicalSequence AnalysisAdd
    BLAST
    Transmembranei113 – 13321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei149 – 16921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei189 – 21123HelicalSequence AnalysisAdd
    BLAST
    Transmembranei217 – 23721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei274 – 29421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei311 – 33121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei349 – 36921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei392 – 41221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei415 – 43521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei451 – 47121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0814.
    GeneTreeiENSGT00710000106531.
    HOVERGENiHBG059571.
    InParanoidiQ8K2P7.
    KOiK14990.
    OMAiIPCIVSE.
    OrthoDBiEOG7WHH9J.
    PhylomeDBiQ8K2P7.
    TreeFamiTF328787.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8K2P7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMHFKSGLEL TELQNMTVPE DDNVSNDSND FTEVENGQIN SKFISDRESR    50
    RSLTNSHLEK RKCDEYIPGT TSLGMSVFNL SNAIMGSGIL GLAFALANTG 100
    ILLFLILLTS VTLLSIYSIN LLLICSKETG CMVYEKLGEQ VFGTTGKLVI 150
    FGATSLQNTG AMLSYLFIVK NELPSAIKSL MGEEDAFSAW YVDGRVLVVM 200
    VTFGIILPLC LLKNLGYLGY TSGFSLSCMM FFLIVVIYKK FQTPCMSVEQ 250
    NSTVSANVTD ACTPKYVTFN SKTVYALPTI AFAFVCHPSV LPIYSELKDR 300
    SQKKMQMVSN ISFFAMFVMY FLTAIFGYLT FYEKVQSDLL HKYQSTGDIL 350
    ILTVRLAVIV AVILTVPVLF FTVRSSLFEL AKKTKFHLCR HVLVTIILLI 400
    IINLLVIFIP SMKDIFGVVG VTSANMLIFI LPSSLYLKIT NQDGDKGTQR 450
    IWAALFLGLG VLFSLISIPL VIYDWACSSG TDEGH 485
    Length:485
    Mass (Da):53,795
    Last modified:October 1, 2002 - v1
    Checksum:i1171E1458818977C
    GO
    Isoform 2 (identifier: Q8K2P7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         333-344: EKVQSDLLHKYQ → GKLLPLAMPICT
         345-485: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:344
    Mass (Da):38,112
    Checksum:i1AFA524A4E9C22AE
    GO
    Isoform 3 (identifier: Q8K2P7-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         67-72: IPGTTS → VSIICM
         73-485: Missing.

    Show »
    Length:72
    Mass (Da):8,358
    Checksum:i01A6F7B92D9D8741
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti48 – 481E → G in BAE38067. (PubMed:16141072)Curated
    Sequence conflicti244 – 2441P → H in BAC33064. (PubMed:16141072)Curated
    Sequence conflicti354 – 3541V → A in AAG43433. (PubMed:11325958)Curated
    Sequence conflicti360 – 3601V → A in AAG43433. (PubMed:11325958)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei67 – 726IPGTTS → VSIICM in isoform 3. 1 PublicationVSP_029305
    Alternative sequencei73 – 485413Missing in isoform 3. 1 PublicationVSP_029306Add
    BLAST
    Alternative sequencei333 – 34412EKVQS…LHKYQ → GKLLPLAMPICT in isoform 2. 1 PublicationVSP_029307Add
    BLAST
    Alternative sequencei345 – 485141Missing in isoform 2. 1 PublicationVSP_029308Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF184240 mRNA. Translation: AAG43433.2.
    AK029189 mRNA. Translation: BAC26341.1.
    AK034130 mRNA. Translation: BAC28597.1.
    AK035098 mRNA. Translation: BAC28944.1.
    AK047459 mRNA. Translation: BAC33064.1.
    AK049294 mRNA. Translation: BAC33663.1.
    AK050914 mRNA. Translation: BAC34457.1.
    AK081724 mRNA. Translation: BAC38310.1.
    AK162612 mRNA. Translation: BAE36989.1.
    AK163914 mRNA. Translation: BAE37532.1.
    AK165188 mRNA. Translation: BAE38067.1.
    BC030378 mRNA. Translation: AAH30378.1.
    BC066815 mRNA. Translation: AAH66815.1.
    CCDSiCCDS27777.1. [Q8K2P7-1]
    RefSeqiNP_001159928.1. NM_001166456.1. [Q8K2P7-1]
    NP_001159930.1. NM_001166458.1. [Q8K2P7-1]
    NP_598847.2. NM_134086.4. [Q8K2P7-1]
    XP_006520298.1. XM_006520235.1. [Q8K2P7-1]
    UniGeneiMm.103568.

    Genome annotation databases

    EnsembliENSMUST00000088452; ENSMUSP00000085799; ENSMUSG00000023169. [Q8K2P7-1]
    ENSMUST00000088454; ENSMUSP00000085801; ENSMUSG00000023169. [Q8K2P7-1]
    ENSMUST00000100262; ENSMUSP00000097833; ENSMUSG00000023169. [Q8K2P7-1]
    GeneIDi105727.
    KEGGimmu:105727.
    UCSCiuc007xkh.2. mouse. [Q8K2P7-1]
    uc007xkk.2. mouse. [Q8K2P7-2]
    uc007xkl.2. mouse. [Q8K2P7-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF184240 mRNA. Translation: AAG43433.2 .
    AK029189 mRNA. Translation: BAC26341.1 .
    AK034130 mRNA. Translation: BAC28597.1 .
    AK035098 mRNA. Translation: BAC28944.1 .
    AK047459 mRNA. Translation: BAC33064.1 .
    AK049294 mRNA. Translation: BAC33663.1 .
    AK050914 mRNA. Translation: BAC34457.1 .
    AK081724 mRNA. Translation: BAC38310.1 .
    AK162612 mRNA. Translation: BAE36989.1 .
    AK163914 mRNA. Translation: BAE37532.1 .
    AK165188 mRNA. Translation: BAE38067.1 .
    BC030378 mRNA. Translation: AAH30378.1 .
    BC066815 mRNA. Translation: AAH66815.1 .
    CCDSi CCDS27777.1. [Q8K2P7-1 ]
    RefSeqi NP_001159928.1. NM_001166456.1. [Q8K2P7-1 ]
    NP_001159930.1. NM_001166458.1. [Q8K2P7-1 ]
    NP_598847.2. NM_134086.4. [Q8K2P7-1 ]
    XP_006520298.1. XM_006520235.1. [Q8K2P7-1 ]
    UniGenei Mm.103568.

    3D structure databases

    ProteinModelPortali Q8K2P7.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8K2P7.

    Proteomic databases

    PRIDEi Q8K2P7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000088452 ; ENSMUSP00000085799 ; ENSMUSG00000023169 . [Q8K2P7-1 ]
    ENSMUST00000088454 ; ENSMUSP00000085801 ; ENSMUSG00000023169 . [Q8K2P7-1 ]
    ENSMUST00000100262 ; ENSMUSP00000097833 ; ENSMUSG00000023169 . [Q8K2P7-1 ]
    GeneIDi 105727.
    KEGGi mmu:105727.
    UCSCi uc007xkh.2. mouse. [Q8K2P7-1 ]
    uc007xkk.2. mouse. [Q8K2P7-2 ]
    uc007xkl.2. mouse. [Q8K2P7-3 ]

    Organism-specific databases

    CTDi 81539.
    MGIi MGI:2145895. Slc38a1.

    Phylogenomic databases

    eggNOGi COG0814.
    GeneTreei ENSGT00710000106531.
    HOVERGENi HBG059571.
    InParanoidi Q8K2P7.
    KOi K14990.
    OMAi IPCIVSE.
    OrthoDBi EOG7WHH9J.
    PhylomeDBi Q8K2P7.
    TreeFami TF328787.

    Miscellaneous databases

    NextBioi 357848.
    PROi Q8K2P7.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8K2P7.
    CleanExi MM_SLC38A1.
    Genevestigatori Q8K2P7.

    Family and domain databases

    InterProi IPR013057. AA_transpt_TM.
    [Graphical view ]
    Pfami PF01490. Aa_trans. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of an N-system amino acid transporter expressed in retina and its involvement in glutamine transport."
      Gu S., Roderick H.L., Camacho P., Jiang J.X.
      J. Biol. Chem. 276:24137-24144(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Cerebellum, Diencephalon, Embryo, Embryonic head, Embryonic stem cell and Head.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Strain: CD-1 and FVB/N-3.
      Tissue: Mammary tumor and Neural stem cell.
    4. "A critical role for system A amino acid transport in the regulation of dendritic development by brain-derived neurotrophic factor (BDNF)."
      Burkhalter J., Fiumelli H., Erickson J.D., Martin J.-L.
      J. Biol. Chem. 282:5152-5159(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY BDNF.

    Entry informationi

    Entry nameiS38A1_MOUSE
    AccessioniPrimary (citable) accession number: Q8K2P7
    Secondary accession number(s): Q3TNM1
    , Q3TQ53, Q6NXZ2, Q8BHI3, Q8BXE2, Q99PR1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3