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Protein

Arf-GAP domain and FG repeat-containing protein 1

Gene

Agfg1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for vesicle docking or fusion during acrosome biogenesis. May play a role in RNA trafficking or localization.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri29 – 5224C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • acrosome assembly Source: MGI
  • intermediate filament organization Source: MGI
  • multicellular organism development Source: UniProtKB-KW
  • spermatid nucleus differentiation Source: MGI
  • transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis, Transport

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP domain and FG repeat-containing protein 1
Alternative name(s):
HIV-1 Rev-binding protein homolog
Nucleoporin-like protein RIP
Gene namesi
Name:Agfg1
Synonyms:Hrb, Rip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1333754. Agfg1.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasmic vesicle By similarity

  • Note: Associated with the cytosolic surface of proacrosomic vesicles of early round spermatids.1 Publication

GO - Cellular componenti

  • cell projection Source: MGI
  • cytoplasmic, membrane-bounded vesicle Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • neuronal cell body Source: MGI
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice have a normal life-span and show no apparent abnormalities. Females display normal fertility but males are infertile due to a lack of acrosome in their spermatozoa.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 561561Arf-GAP domain and FG repeat-containing protein 1PRO_0000204905Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei167 – 1671PhosphoserineBy similarity
Modified residuei177 – 1771PhosphothreonineCombined sources
Modified residuei181 – 1811PhosphoserineCombined sources
Modified residuei362 – 3621PhosphoserineBy similarity
Glycosylationi367 – 3671O-linked (GlcNAc)By similarity

Post-translational modificationi

O-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ8K2K6.
MaxQBiQ8K2K6.
PaxDbiQ8K2K6.
PeptideAtlasiQ8K2K6.
PRIDEiQ8K2K6.

PTM databases

iPTMnetiQ8K2K6.
PhosphoSiteiQ8K2K6.

Expressioni

Developmental stagei

Highly expressed during spermiogenesis.1 Publication

Gene expression databases

BgeeiQ8K2K6.
ExpressionAtlasiQ8K2K6. baseline and differential.
GenevisibleiQ8K2K6. MM.

Interactioni

Subunit structurei

Interacts with FCHO1 (By similarity). Interacts with EPS15R and EPS15.By similarity2 Publications

Protein-protein interaction databases

BioGridi200417. 2 interactions.
IntActiQ8K2K6. 1 interaction.
MINTiMINT-4104441.
STRINGi10090.ENSMUSP00000070250.

Structurei

3D structure databases

ProteinModelPortaliQ8K2K6.
SMRiQ8K2K6. Positions 14-134, 153-186.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 135125Arf-GAPPROSITE-ProRule annotationAdd
BLAST

Domaini

Contains FG repeats.

Sequence similaritiesi

Contains 1 Arf-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri29 – 5224C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IUMA. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00560000077249.
HOGENOMiHOG000253009.
HOVERGENiHBG006551.
InParanoidiQ8K2K6.
KOiK15044.
OrthoDBiEOG7W9RW0.
PhylomeDBiQ8K2K6.
TreeFamiTF325357.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 4 (identifier: Q8K2K6-4) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASAKRKQE EKHLKMLRDM TGLPHNRKCF DCDQRGPTYV NMTVGSFVCT
60 70 80 90 100
SCSGSLRGLN PPHRVKSISM TTFTQQEIEF LQKHGNEVCK QIWLGLFDDR
110 120 130 140 150
SSAIPDFRDP QKVKEFLQEK YEKKRWYVPP EQAKVVASVH ASISGSSASS
160 170 180 190 200
TSSTPEVKPL KSLLGESAPA LHLNKGTPSQ SPVVGRSQGQ QQEKKQFDLL
210 220 230 240 250
SDLGSDIFAA PAPQSTATAN FANFAHFNSH AAQNSANADF ANFDAFGQSS
260 270 280 290 300
GSSNFGGFPT ASHSSFQPQT TGGSAGSVNA NFAHFDNFPK SSSADFGTFS
310 320 330 340 350
TSQSHQTAST VSKVSTNKAG LQTADKYAAL ANLDNIFSAG QGGDQGSGFG
360 370 380 390 400
TTGKAPVGSV VSVPSHSSAS SDKYAALAEL DSVFSSAATS SNAYTPTSNA
410 420 430 440 450
SSSVFGTVPV GASAQTQPAS SGPAPFGATP STNPFVAATG PSAASSTNPF
460 470 480 490 500
QTNARGATAA TFGTASMSMP AGFGTPAQYS LPTSFSGSFQ QPAFPAQAAF
510 520 530 540 550
PQQTAFSQQP NGAGFATFGQ TKPVVTPFGQ VAAAGVSSNP FMTGAPTGQL
560
PTGSSSTNPF L
Length:561
Mass (Da):58,043
Last modified:March 21, 2006 - v2
Checksum:iB612D82F0051ECFF
GO
Isoform 1 (identifier: Q8K2K6-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     513-514: Missing.

Show »
Length:559
Mass (Da):57,915
Checksum:iE478ABCCD75F011F
GO
Isoform 2 (identifier: Q8K2K6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     342-362: Missing.

Note: No experimental confirmation available.
Show »
Length:540
Mass (Da):56,196
Checksum:i0A3C8342CF763265
GO
Isoform 3 (identifier: Q8K2K6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     428-458: Missing.

Show »
Length:530
Mass (Da):55,068
Checksum:iDFFDBA80DF922AA0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 43AAS → GGR in BAC33736 (PubMed:16141072).Curated
Sequence conflicti9 – 91Q → P in BAC33736 (PubMed:16141072).Curated
Sequence conflicti34 – 341Q → H in BAC33736 (PubMed:16141072).Curated
Sequence conflicti43 – 431T → R in BAC33736 (PubMed:16141072).Curated
Sequence conflicti57 – 571R → K in BAC33736 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei342 – 36221Missing in isoform 2. 1 PublicationVSP_010664Add
BLAST
Alternative sequencei428 – 45831Missing in isoform 3. 1 PublicationVSP_010665Add
BLAST
Alternative sequencei513 – 5142Missing in isoform 1. 2 PublicationsVSP_017602

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049400 mRNA. Translation: BAC33736.1.
AK029917 mRNA. Translation: BAC26675.1.
AK048326 mRNA. Translation: BAC33303.1.
AF057287 mRNA. Translation: AAC97477.1.
BC031154 mRNA. Translation: AAH31154.1.
CCDSiCCDS15100.1. [Q8K2K6-1]
CCDS78634.1. [Q8K2K6-3]
RefSeqiNP_001297642.1. NM_001310713.1. [Q8K2K6-3]
NP_034602.1. NM_010472.3. [Q8K2K6-1]
XP_006496496.1. XM_006496433.2. [Q8K2K6-4]
UniGeneiMm.392569.
Mm.433409.

Genome annotation databases

EnsembliENSMUST00000063380; ENSMUSP00000070250; ENSMUSG00000026159. [Q8K2K6-2]
ENSMUST00000113444; ENSMUSP00000109071; ENSMUSG00000026159. [Q8K2K6-3]
ENSMUST00000186302; ENSMUSP00000140785; ENSMUSG00000026159. [Q8K2K6-4]
ENSMUST00000189220; ENSMUSP00000140170; ENSMUSG00000026159. [Q8K2K6-1]
GeneIDi15463.
KEGGimmu:15463.
UCSCiuc007bse.1. mouse. [Q8K2K6-1]
uc007bsf.1. mouse. [Q8K2K6-2]
uc007bsg.1. mouse. [Q8K2K6-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049400 mRNA. Translation: BAC33736.1.
AK029917 mRNA. Translation: BAC26675.1.
AK048326 mRNA. Translation: BAC33303.1.
AF057287 mRNA. Translation: AAC97477.1.
BC031154 mRNA. Translation: AAH31154.1.
CCDSiCCDS15100.1. [Q8K2K6-1]
CCDS78634.1. [Q8K2K6-3]
RefSeqiNP_001297642.1. NM_001310713.1. [Q8K2K6-3]
NP_034602.1. NM_010472.3. [Q8K2K6-1]
XP_006496496.1. XM_006496433.2. [Q8K2K6-4]
UniGeneiMm.392569.
Mm.433409.

3D structure databases

ProteinModelPortaliQ8K2K6.
SMRiQ8K2K6. Positions 14-134, 153-186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200417. 2 interactions.
IntActiQ8K2K6. 1 interaction.
MINTiMINT-4104441.
STRINGi10090.ENSMUSP00000070250.

PTM databases

iPTMnetiQ8K2K6.
PhosphoSiteiQ8K2K6.

Proteomic databases

EPDiQ8K2K6.
MaxQBiQ8K2K6.
PaxDbiQ8K2K6.
PeptideAtlasiQ8K2K6.
PRIDEiQ8K2K6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063380; ENSMUSP00000070250; ENSMUSG00000026159. [Q8K2K6-2]
ENSMUST00000113444; ENSMUSP00000109071; ENSMUSG00000026159. [Q8K2K6-3]
ENSMUST00000186302; ENSMUSP00000140785; ENSMUSG00000026159. [Q8K2K6-4]
ENSMUST00000189220; ENSMUSP00000140170; ENSMUSG00000026159. [Q8K2K6-1]
GeneIDi15463.
KEGGimmu:15463.
UCSCiuc007bse.1. mouse. [Q8K2K6-1]
uc007bsf.1. mouse. [Q8K2K6-2]
uc007bsg.1. mouse. [Q8K2K6-3]

Organism-specific databases

CTDi3267.
MGIiMGI:1333754. Agfg1.

Phylogenomic databases

eggNOGiENOG410IUMA. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00560000077249.
HOGENOMiHOG000253009.
HOVERGENiHBG006551.
InParanoidiQ8K2K6.
KOiK15044.
OrthoDBiEOG7W9RW0.
PhylomeDBiQ8K2K6.
TreeFamiTF325357.

Miscellaneous databases

PROiQ8K2K6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K2K6.
ExpressionAtlasiQ8K2K6. baseline and differential.
GenevisibleiQ8K2K6. MM.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4).
    Strain: C57BL/6J.
    Tissue: Head and Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. "Eps15R is a tyrosine kinase substrate with characteristics of a docking protein possibly involved in coated pits-mediated internalization."
    Coda L., Salcini A.E., Confalonieri S., Pelicci G., Sorkina T., Sorkin A., Pelicci P.G., Di Fiore P.P.
    J. Biol. Chem. 273:3003-3012(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EPS15R.
  4. Cited for: FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, INTERACTION WITH EPS15, DISRUPTION PHENOTYPE.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-177 AND SER-181, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-177 AND SER-181, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-177 AND SER-181, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiAGFG1_MOUSE
AccessioniPrimary (citable) accession number: Q8K2K6
Secondary accession number(s): O70448, Q8BQL5, Q8CDK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: March 21, 2006
Last modified: July 6, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.