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Protein

Arf-GAP domain and FG repeat-containing protein 1

Gene

Agfg1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for vesicle docking or fusion during acrosome biogenesis. May play a role in RNA trafficking or localization.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri29 – 52C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

  • acrosome assembly Source: MGI
  • intermediate filament organization Source: MGI
  • multicellular organism development Source: UniProtKB-KW
  • spermatid nucleus differentiation Source: MGI
  • transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis, Transport

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP domain and FG repeat-containing protein 1
Alternative name(s):
HIV-1 Rev-binding protein homolog
Nucleoporin-like protein RIP
Gene namesi
Name:Agfg1
Synonyms:Hrb, Rip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1333754. Agfg1.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasmic vesicle By similarity

  • Note: Associated with the cytosolic surface of proacrosomic vesicles of early round spermatids.1 Publication

GO - Cellular componenti

  • cell projection Source: MGI
  • cytoplasmic, membrane-bounded vesicle Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • neuronal cell body Source: MGI
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice have a normal life-span and show no apparent abnormalities. Females display normal fertility but males are infertile due to a lack of acrosome in their spermatozoa.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002049051 – 561Arf-GAP domain and FG repeat-containing protein 1Add BLAST561

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei167PhosphoserineBy similarity1
Modified residuei177PhosphothreonineCombined sources1
Modified residuei181PhosphoserineCombined sources1
Modified residuei362PhosphoserineBy similarity1
Glycosylationi367O-linked (GlcNAc)By similarity1

Post-translational modificationi

O-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8K2K6.
PaxDbiQ8K2K6.
PeptideAtlasiQ8K2K6.
PRIDEiQ8K2K6.

PTM databases

iPTMnetiQ8K2K6.
PhosphoSitePlusiQ8K2K6.

Expressioni

Developmental stagei

Highly expressed during spermiogenesis.1 Publication

Gene expression databases

BgeeiENSMUSG00000026159.
ExpressionAtlasiQ8K2K6. baseline and differential.
GenevisibleiQ8K2K6. MM.

Interactioni

Subunit structurei

Interacts with FCHO1 (By similarity). Interacts with EPS15R and EPS15.By similarity2 Publications

Protein-protein interaction databases

BioGridi200417. 2 interactors.
IntActiQ8K2K6. 1 interactor.
MINTiMINT-4104441.
STRINGi10090.ENSMUSP00000070250.

Structurei

3D structure databases

ProteinModelPortaliQ8K2K6.
SMRiQ8K2K6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 135Arf-GAPPROSITE-ProRule annotationAdd BLAST125

Domaini

Contains FG repeats.

Sequence similaritiesi

Contains 1 Arf-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri29 – 52C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IUMA. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00560000077249.
HOGENOMiHOG000253009.
HOVERGENiHBG006551.
InParanoidiQ8K2K6.
KOiK15044.
PhylomeDBiQ8K2K6.
TreeFamiTF325357.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 4 (identifier: Q8K2K6-4) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASAKRKQE EKHLKMLRDM TGLPHNRKCF DCDQRGPTYV NMTVGSFVCT
60 70 80 90 100
SCSGSLRGLN PPHRVKSISM TTFTQQEIEF LQKHGNEVCK QIWLGLFDDR
110 120 130 140 150
SSAIPDFRDP QKVKEFLQEK YEKKRWYVPP EQAKVVASVH ASISGSSASS
160 170 180 190 200
TSSTPEVKPL KSLLGESAPA LHLNKGTPSQ SPVVGRSQGQ QQEKKQFDLL
210 220 230 240 250
SDLGSDIFAA PAPQSTATAN FANFAHFNSH AAQNSANADF ANFDAFGQSS
260 270 280 290 300
GSSNFGGFPT ASHSSFQPQT TGGSAGSVNA NFAHFDNFPK SSSADFGTFS
310 320 330 340 350
TSQSHQTAST VSKVSTNKAG LQTADKYAAL ANLDNIFSAG QGGDQGSGFG
360 370 380 390 400
TTGKAPVGSV VSVPSHSSAS SDKYAALAEL DSVFSSAATS SNAYTPTSNA
410 420 430 440 450
SSSVFGTVPV GASAQTQPAS SGPAPFGATP STNPFVAATG PSAASSTNPF
460 470 480 490 500
QTNARGATAA TFGTASMSMP AGFGTPAQYS LPTSFSGSFQ QPAFPAQAAF
510 520 530 540 550
PQQTAFSQQP NGAGFATFGQ TKPVVTPFGQ VAAAGVSSNP FMTGAPTGQL
560
PTGSSSTNPF L
Length:561
Mass (Da):58,043
Last modified:March 21, 2006 - v2
Checksum:iB612D82F0051ECFF
GO
Isoform 1 (identifier: Q8K2K6-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     513-514: Missing.

Show »
Length:559
Mass (Da):57,915
Checksum:iE478ABCCD75F011F
GO
Isoform 2 (identifier: Q8K2K6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     342-362: Missing.

Note: No experimental confirmation available.
Show »
Length:540
Mass (Da):56,196
Checksum:i0A3C8342CF763265
GO
Isoform 3 (identifier: Q8K2K6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     428-458: Missing.

Show »
Length:530
Mass (Da):55,068
Checksum:iDFFDBA80DF922AA0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2 – 4AAS → GGR in BAC33736 (PubMed:16141072).Curated3
Sequence conflicti9Q → P in BAC33736 (PubMed:16141072).Curated1
Sequence conflicti34Q → H in BAC33736 (PubMed:16141072).Curated1
Sequence conflicti43T → R in BAC33736 (PubMed:16141072).Curated1
Sequence conflicti57R → K in BAC33736 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010664342 – 362Missing in isoform 2. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_010665428 – 458Missing in isoform 3. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_017602513 – 514Missing in isoform 1. 2 Publications2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049400 mRNA. Translation: BAC33736.1.
AK029917 mRNA. Translation: BAC26675.1.
AK048326 mRNA. Translation: BAC33303.1.
AF057287 mRNA. Translation: AAC97477.1.
BC031154 mRNA. Translation: AAH31154.1.
CCDSiCCDS15100.1. [Q8K2K6-1]
CCDS78634.1. [Q8K2K6-3]
RefSeqiNP_001297642.1. NM_001310713.1. [Q8K2K6-3]
NP_034602.1. NM_010472.3. [Q8K2K6-1]
XP_006496496.1. XM_006496433.3. [Q8K2K6-4]
UniGeneiMm.392569.
Mm.433409.

Genome annotation databases

EnsembliENSMUST00000063380; ENSMUSP00000070250; ENSMUSG00000026159. [Q8K2K6-2]
ENSMUST00000113444; ENSMUSP00000109071; ENSMUSG00000026159. [Q8K2K6-3]
ENSMUST00000186302; ENSMUSP00000140785; ENSMUSG00000026159. [Q8K2K6-4]
ENSMUST00000189220; ENSMUSP00000140170; ENSMUSG00000026159. [Q8K2K6-1]
GeneIDi15463.
KEGGimmu:15463.
UCSCiuc007bse.1. mouse. [Q8K2K6-1]
uc007bsf.1. mouse. [Q8K2K6-2]
uc007bsg.1. mouse. [Q8K2K6-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049400 mRNA. Translation: BAC33736.1.
AK029917 mRNA. Translation: BAC26675.1.
AK048326 mRNA. Translation: BAC33303.1.
AF057287 mRNA. Translation: AAC97477.1.
BC031154 mRNA. Translation: AAH31154.1.
CCDSiCCDS15100.1. [Q8K2K6-1]
CCDS78634.1. [Q8K2K6-3]
RefSeqiNP_001297642.1. NM_001310713.1. [Q8K2K6-3]
NP_034602.1. NM_010472.3. [Q8K2K6-1]
XP_006496496.1. XM_006496433.3. [Q8K2K6-4]
UniGeneiMm.392569.
Mm.433409.

3D structure databases

ProteinModelPortaliQ8K2K6.
SMRiQ8K2K6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200417. 2 interactors.
IntActiQ8K2K6. 1 interactor.
MINTiMINT-4104441.
STRINGi10090.ENSMUSP00000070250.

PTM databases

iPTMnetiQ8K2K6.
PhosphoSitePlusiQ8K2K6.

Proteomic databases

MaxQBiQ8K2K6.
PaxDbiQ8K2K6.
PeptideAtlasiQ8K2K6.
PRIDEiQ8K2K6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063380; ENSMUSP00000070250; ENSMUSG00000026159. [Q8K2K6-2]
ENSMUST00000113444; ENSMUSP00000109071; ENSMUSG00000026159. [Q8K2K6-3]
ENSMUST00000186302; ENSMUSP00000140785; ENSMUSG00000026159. [Q8K2K6-4]
ENSMUST00000189220; ENSMUSP00000140170; ENSMUSG00000026159. [Q8K2K6-1]
GeneIDi15463.
KEGGimmu:15463.
UCSCiuc007bse.1. mouse. [Q8K2K6-1]
uc007bsf.1. mouse. [Q8K2K6-2]
uc007bsg.1. mouse. [Q8K2K6-3]

Organism-specific databases

CTDi3267.
MGIiMGI:1333754. Agfg1.

Phylogenomic databases

eggNOGiENOG410IUMA. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00560000077249.
HOGENOMiHOG000253009.
HOVERGENiHBG006551.
InParanoidiQ8K2K6.
KOiK15044.
PhylomeDBiQ8K2K6.
TreeFamiTF325357.

Enzyme and pathway databases

ReactomeiR-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

PROiQ8K2K6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026159.
ExpressionAtlasiQ8K2K6. baseline and differential.
GenevisibleiQ8K2K6. MM.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGFG1_MOUSE
AccessioniPrimary (citable) accession number: Q8K2K6
Secondary accession number(s): O70448, Q8BQL5, Q8CDK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: March 21, 2006
Last modified: November 2, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.