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Protein

Arf-GAP domain and FG repeat-containing protein 1

Gene

Agfg1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for vesicle docking or fusion during acrosome biogenesis. May play a role in RNA trafficking or localization.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri29 – 52C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

  • acrosome assembly Source: MGI
  • intermediate filament organization Source: MGI
  • multicellular organism development Source: UniProtKB-KW
  • spermatid nucleus differentiation Source: MGI

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processDifferentiation, Spermatogenesis, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP domain and FG repeat-containing protein 1
Alternative name(s):
HIV-1 Rev-binding protein homolog
Nucleoporin-like protein RIP
Gene namesi
Name:Agfg1
Synonyms:Hrb, Rip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1333754 Agfg1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice have a normal life-span and show no apparent abnormalities. Females display normal fertility but males are infertile due to a lack of acrosome in their spermatozoa.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002049051 – 561Arf-GAP domain and FG repeat-containing protein 1Add BLAST561

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei167PhosphoserineBy similarity1
Modified residuei177PhosphothreonineCombined sources1
Modified residuei181PhosphoserineCombined sources1
Modified residuei362PhosphoserineBy similarity1
Glycosylationi367O-linked (GlcNAc) serineBy similarity1

Post-translational modificationi

O-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8K2K6
PaxDbiQ8K2K6
PeptideAtlasiQ8K2K6
PRIDEiQ8K2K6

PTM databases

iPTMnetiQ8K2K6
PhosphoSitePlusiQ8K2K6

Expressioni

Developmental stagei

Highly expressed during spermiogenesis.1 Publication

Gene expression databases

BgeeiENSMUSG00000026159
ExpressionAtlasiQ8K2K6 baseline and differential
GenevisibleiQ8K2K6 MM

Interactioni

Subunit structurei

Interacts with FCHO1 (By similarity). Interacts with EPS15R and EPS15.By similarity2 Publications

Protein-protein interaction databases

BioGridi200417, 2 interactors
ELMiQ8K2K6
IntActiQ8K2K6, 1 interactor
MINTiQ8K2K6
STRINGi10090.ENSMUSP00000070250

Structurei

3D structure databases

ProteinModelPortaliQ8K2K6
SMRiQ8K2K6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 135Arf-GAPPROSITE-ProRule annotationAdd BLAST125

Domaini

Contains FG repeats.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri29 – 52C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IUMA Eukaryota
COG5347 LUCA
GeneTreeiENSGT00560000077249
HOGENOMiHOG000253009
HOVERGENiHBG006551
InParanoidiQ8K2K6
KOiK15044
PhylomeDBiQ8K2K6
TreeFamiTF325357

Family and domain databases

Gene3Di3.30.40.160, 1 hit
InterProiView protein in InterPro
IPR037278 ARFGAP/RecO
IPR001164 ArfGAP_dom
IPR038508 ArfGAP_dom_sf
PfamiView protein in Pfam
PF01412 ArfGap, 1 hit
PRINTSiPR00405 REVINTRACTNG
SMARTiView protein in SMART
SM00105 ArfGap, 1 hit
SUPFAMiSSF57863 SSF57863, 1 hit
PROSITEiView protein in PROSITE
PS50115 ARFGAP, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 4 (identifier: Q8K2K6-4) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASAKRKQE EKHLKMLRDM TGLPHNRKCF DCDQRGPTYV NMTVGSFVCT
60 70 80 90 100
SCSGSLRGLN PPHRVKSISM TTFTQQEIEF LQKHGNEVCK QIWLGLFDDR
110 120 130 140 150
SSAIPDFRDP QKVKEFLQEK YEKKRWYVPP EQAKVVASVH ASISGSSASS
160 170 180 190 200
TSSTPEVKPL KSLLGESAPA LHLNKGTPSQ SPVVGRSQGQ QQEKKQFDLL
210 220 230 240 250
SDLGSDIFAA PAPQSTATAN FANFAHFNSH AAQNSANADF ANFDAFGQSS
260 270 280 290 300
GSSNFGGFPT ASHSSFQPQT TGGSAGSVNA NFAHFDNFPK SSSADFGTFS
310 320 330 340 350
TSQSHQTAST VSKVSTNKAG LQTADKYAAL ANLDNIFSAG QGGDQGSGFG
360 370 380 390 400
TTGKAPVGSV VSVPSHSSAS SDKYAALAEL DSVFSSAATS SNAYTPTSNA
410 420 430 440 450
SSSVFGTVPV GASAQTQPAS SGPAPFGATP STNPFVAATG PSAASSTNPF
460 470 480 490 500
QTNARGATAA TFGTASMSMP AGFGTPAQYS LPTSFSGSFQ QPAFPAQAAF
510 520 530 540 550
PQQTAFSQQP NGAGFATFGQ TKPVVTPFGQ VAAAGVSSNP FMTGAPTGQL
560
PTGSSSTNPF L
Length:561
Mass (Da):58,043
Last modified:March 21, 2006 - v2
Checksum:iB612D82F0051ECFF
GO
Isoform 1 (identifier: Q8K2K6-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     513-514: Missing.

Show »
Length:559
Mass (Da):57,915
Checksum:iE478ABCCD75F011F
GO
Isoform 2 (identifier: Q8K2K6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     342-362: Missing.

Note: No experimental confirmation available.
Show »
Length:540
Mass (Da):56,196
Checksum:i0A3C8342CF763265
GO
Isoform 3 (identifier: Q8K2K6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     428-458: Missing.

Show »
Length:530
Mass (Da):55,068
Checksum:iDFFDBA80DF922AA0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2 – 4AAS → GGR in BAC33736 (PubMed:16141072).Curated3
Sequence conflicti9Q → P in BAC33736 (PubMed:16141072).Curated1
Sequence conflicti34Q → H in BAC33736 (PubMed:16141072).Curated1
Sequence conflicti43T → R in BAC33736 (PubMed:16141072).Curated1
Sequence conflicti57R → K in BAC33736 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010664342 – 362Missing in isoform 2. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_010665428 – 458Missing in isoform 3. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_017602513 – 514Missing in isoform 1. 2 Publications2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049400 mRNA Translation: BAC33736.1
AK029917 mRNA Translation: BAC26675.1
AK048326 mRNA Translation: BAC33303.1
AF057287 mRNA Translation: AAC97477.1
BC031154 mRNA Translation: AAH31154.1
CCDSiCCDS15100.1 [Q8K2K6-1]
CCDS78634.1 [Q8K2K6-3]
RefSeqiNP_001297642.1, NM_001310713.1 [Q8K2K6-3]
NP_034602.1, NM_010472.3 [Q8K2K6-1]
XP_006496496.1, XM_006496433.3 [Q8K2K6-4]
UniGeneiMm.392569
Mm.433409

Genome annotation databases

EnsembliENSMUST00000063380; ENSMUSP00000070250; ENSMUSG00000026159 [Q8K2K6-2]
ENSMUST00000113444; ENSMUSP00000109071; ENSMUSG00000026159 [Q8K2K6-3]
ENSMUST00000186302; ENSMUSP00000140785; ENSMUSG00000026159 [Q8K2K6-4]
ENSMUST00000189220; ENSMUSP00000140170; ENSMUSG00000026159 [Q8K2K6-1]
GeneIDi15463
KEGGimmu:15463
UCSCiuc007bse.1 mouse [Q8K2K6-1]
uc007bsf.1 mouse [Q8K2K6-2]
uc007bsg.1 mouse [Q8K2K6-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAGFG1_MOUSE
AccessioniPrimary (citable) accession number: Q8K2K6
Secondary accession number(s): O70448, Q8BQL5, Q8CDK9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: March 21, 2006
Last modified: March 28, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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