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Q8K2I4

- MANBA_MOUSE

UniProt

Q8K2I4 - MANBA_MOUSE

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Protein

Beta-mannosidase

Gene
Manba, Bmn
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides By similarity.By similarity

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei457 – 4571Proton donor By similarityBy similarity

GO - Molecular functioni

  1. beta-mannosidase activity Source: MGI
  2. mannose binding Source: Ensembl

GO - Biological processi

  1. glycoprotein catabolic process Source: MGI
  2. mannan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

UniPathwayiUPA00280.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-mannosidase (EC:3.2.1.25)
Alternative name(s):
Lysosomal beta A mannosidase
Mannanase
Short name:
Mannase
Gene namesi
Name:Manba
Synonyms:Bmn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:88175. Manba.

Subcellular locationi

Lysosome By similarity By similarity

GO - Cellular componenti

  1. lysosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Disruption phenotypei

Mice show vacuolation in the central nervous system and accumulation of disaccharides in brain and epididymis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed predictionAdd
BLAST
Chaini20 – 879860Beta-mannosidasePRO_0000250613Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...) Reviewed prediction
Glycosylationi77 – 771N-linked (GlcNAc...) Reviewed prediction
Glycosylationi89 – 891N-linked (GlcNAc...) Reviewed prediction
Glycosylationi113 – 1131N-linked (GlcNAc...) Reviewed prediction
Glycosylationi226 – 2261N-linked (GlcNAc...) Reviewed prediction
Glycosylationi297 – 2971N-linked (GlcNAc...) Reviewed prediction
Glycosylationi302 – 3021N-linked (GlcNAc...) Reviewed prediction
Glycosylationi736 – 7361N-linked (GlcNAc...) Reviewed prediction
Glycosylationi803 – 8031N-linked (GlcNAc...) Reviewed prediction
Glycosylationi807 – 8071N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8K2I4.
PaxDbiQ8K2I4.
PRIDEiQ8K2I4.

PTM databases

PhosphoSiteiQ8K2I4.

Expressioni

Tissue specificityi

Highest level in liver, high levels in lung, testis, skin and spleen, moderate level in thymus. Activity found in plasma, kidney, liver, spleen, pancreas, brain, testis, epididymis, heart, lung and skeletal muscle.2 Publications

Gene expression databases

ArrayExpressiQ8K2I4.
BgeeiQ8K2I4.
CleanExiMM_MANBA.
GenevestigatoriQ8K2I4.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029814.

Structurei

3D structure databases

ProteinModelPortaliQ8K2I4.
SMRiQ8K2I4. Positions 25-879.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3250.
GeneTreeiENSGT00390000001670.
HOGENOMiHOG000186862.
HOVERGENiHBG052404.
InParanoidiQ8K2I4.
KOiK01192.
OrthoDBiEOG7S7SD2.
PhylomeDBiQ8K2I4.
TreeFamiTF105723.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view]
PANTHERiPTHR10066:SF12. PTHR10066:SF12. 1 hit.
PfamiPF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 3 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K2I4-1 [UniParc]FASTAAdd to Basket

« Hide

MHLHLLLILA LFRAGCVVAG PSYSLSGSWR VSNGNGSLEL PATVPGYVHS    50
ALHQHGLIQD PYYRFNDLNY RWISLDNWTY STEFKIPFNL SEWQKVKLIF 100
DGVDTVAEIL FNNVTIGKTD NMFTGYSFDI TNVVKDVNSL KLQFRSAVQY 150
AECQSKAHTS YRVPPECPPV EQKGECHVNF IRKAQCSFSW DWGPSFPSQG 200
IWKDVRIEAY NIAHLDYLTF LPVYDNASQA WNIEIKASFD VASSKSVGGQ 250
VTVAIPQLKT QQTNDIELQQ EQRIVKLLVK IRKDVAVETW WPRGHGNQTG 300
YNMTILFALD GGLKIEKAAK VYFRTVQLIE EGIKGSPGLS FYFKINGLPI 350
FLKGSNWIPA DSFQDKVTSD RLQLLFQSVV DANMNTLRVW GGGIYEQDEF 400
YALCDELGIM VWQDFMFASA LYPTEPGFLA SVRKEVTYQV RRLKSHPSII 450
IWSGNNENEV ALSVNWFHVN PRDMKTYIDD YVTLYVKNIR KIVLSEDKSR 500
PFIASSPTNG MKTMEEGWIS YDPYSIQYGD IHFYNYADDC WNWKIFPKAR 550
LVSEYGYQSW PSFSTLEKVS SQEDWAYNSR FSLHRQHHED GNHQMLHQVK 600
MHFKLPQGTD PLRTFKDTIY LTQVMQAQCI KTETEFYLRS RSEIVDGKGH 650
TMGALYWQLN DIWQAPSWAS LEYGGKWKML HYFARRFFAP LLPVGFEDEG 700
VFYVYGVSDL HKDHHTQLTV RLHHWSSPKP LCSLVNSSIV VKAGEAVVLF 750
QMPVSELLKR CRGCTRETCV VSFYFSTDKE LFSPTNYHFL SSLKDAKGLL 800
EANITVNISQ KGNVFVFDLE TSAVAPFVWL DVGSIPGRFS DNGFLMIRKK 850
LSVLFYPWKP TSKSELQQAF SVTSLTDTY 879
Length:879
Mass (Da):100,831
Last modified:October 1, 2002 - v1
Checksum:i1DE203C44557719B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti130 – 1301I → V in AAK18177. 1 Publication
Sequence conflicti130 – 1301I → V in BAE24954. 1 Publication
Sequence conflicti130 – 1301I → V in BAE38715. 1 Publication
Sequence conflicti143 – 1453QFR → RFW in BAE38715. 1 Publication
Sequence conflicti153 – 1531C → R in BAE38715. 1 Publication
Sequence conflicti160 – 1623SYR → RYP in BAE38715. 1 Publication
Sequence conflicti236 – 2361K → E in AAK18177. 1 Publication
Sequence conflicti236 – 2361K → E in BAE24954. 1 Publication
Sequence conflicti236 – 2361K → E in BAE38715. 1 Publication
Sequence conflicti236 – 2361K → E in BAE41690. 1 Publication
Sequence conflicti248 – 2481G → S in AAK18177. 1 Publication
Sequence conflicti248 – 2481G → S in BAE24954. 1 Publication
Sequence conflicti264 – 2641N → H in BAE38715. 1 Publication
Sequence conflicti376 – 3761F → L in BAE24954. 1 Publication
Sequence conflicti491 – 4911K → N in BAE38715. 1 Publication
Sequence conflicti511 – 5111M → I in BAE38715. 1 Publication
Sequence conflicti714 – 7141H → Y in BAE38715. 1 Publication
Sequence conflicti744 – 7441G → R in BAE38715. 1 Publication
Sequence conflicti834 – 8341S → G in BAE38715. 1 Publication
Sequence conflicti848 – 8481R → Q in BAE38715. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF306557 mRNA. Translation: AAK18177.1.
AK010623 mRNA. Translation: BAB27069.1.
AK142165 mRNA. Translation: BAE24954.1.
AK166340 mRNA. Translation: BAE38715.1.
AK170290 mRNA. Translation: BAE41690.1.
BC031409 mRNA. Translation: AAH31409.1.
CCDSiCCDS17857.1.
RefSeqiNP_081564.3. NM_027288.3.
UniGeneiMm.280536.

Genome annotation databases

EnsembliENSMUST00000029814; ENSMUSP00000029814; ENSMUSG00000028164.
GeneIDi110173.
KEGGimmu:110173.
UCSCiuc008rlu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF306557 mRNA. Translation: AAK18177.1 .
AK010623 mRNA. Translation: BAB27069.1 .
AK142165 mRNA. Translation: BAE24954.1 .
AK166340 mRNA. Translation: BAE38715.1 .
AK170290 mRNA. Translation: BAE41690.1 .
BC031409 mRNA. Translation: AAH31409.1 .
CCDSi CCDS17857.1.
RefSeqi NP_081564.3. NM_027288.3.
UniGenei Mm.280536.

3D structure databases

ProteinModelPortali Q8K2I4.
SMRi Q8K2I4. Positions 25-879.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000029814.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

PTM databases

PhosphoSitei Q8K2I4.

Proteomic databases

MaxQBi Q8K2I4.
PaxDbi Q8K2I4.
PRIDEi Q8K2I4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000029814 ; ENSMUSP00000029814 ; ENSMUSG00000028164 .
GeneIDi 110173.
KEGGi mmu:110173.
UCSCi uc008rlu.2. mouse.

Organism-specific databases

CTDi 4126.
MGIi MGI:88175. Manba.

Phylogenomic databases

eggNOGi COG3250.
GeneTreei ENSGT00390000001670.
HOGENOMi HOG000186862.
HOVERGENi HBG052404.
InParanoidi Q8K2I4.
KOi K01192.
OrthoDBi EOG7S7SD2.
PhylomeDBi Q8K2I4.
TreeFami TF105723.

Enzyme and pathway databases

UniPathwayi UPA00280 .

Miscellaneous databases

NextBioi 363463.
PROi Q8K2I4.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8K2I4.
Bgeei Q8K2I4.
CleanExi MM_MANBA.
Genevestigatori Q8K2I4.

Family and domain databases

Gene3Di 2.60.120.260. 2 hits.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view ]
PANTHERi PTHR10066:SF12. PTHR10066:SF12. 1 hit.
Pfami PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 3 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse beta-mannosidase, cDNA cloning, expression, and chromosomal localization."
    Beccari T., Bibi L., Stinchi S., Stirling J.L., Orlacchio A.
    Biosci. Rep. 21:315-323(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Spleen.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Dendritic cell, Embryonic heart, Embryonic stem cell and Mammary gland.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  4. "Beta-mannosidosis mice: a model for the human lysosomal storage disease."
    Zhu M., Lovell K.L., Patterson J.S., Saunders T.L., Hughes E.D., Friderici K.H.
    Hum. Mol. Genet. 15:493-500(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiMANBA_MOUSE
AccessioniPrimary (citable) accession number: Q8K2I4
Secondary accession number(s): Q3TDB3
, Q3TLS7, Q3UQT5, Q99MS1, Q9CRH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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