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Q8K2I4

- MANBA_MOUSE

UniProt

Q8K2I4 - MANBA_MOUSE

Protein

Beta-mannosidase

Gene

Manba

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.By similarity

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei457 – 4571Proton donorBy similarity

    GO - Molecular functioni

    1. beta-mannosidase activity Source: MGI
    2. mannose binding Source: Ensembl

    GO - Biological processi

    1. glycoprotein catabolic process Source: MGI
    2. mannan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    UniPathwayiUPA00280.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-mannosidase (EC:3.2.1.25)
    Alternative name(s):
    Lysosomal beta A mannosidase
    Mannanase
    Short name:
    Mannase
    Gene namesi
    Name:ManbaImported
    Synonyms:Bmn1 Publication
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:88175. Manba.

    Subcellular locationi

    Lysosome By similarity

    GO - Cellular componenti

    1. lysosome Source: MGI

    Keywords - Cellular componenti

    Lysosome

    Pathology & Biotechi

    Disruption phenotypei

    Mice show vacuolation in the central nervous system and accumulation of disaccharides in brain and epididymis.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 879860Beta-mannosidaseSequence AnalysisPRO_0000250613Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi77 – 771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi226 – 2261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi302 – 3021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi736 – 7361N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi803 – 8031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi807 – 8071N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ8K2I4.
    PaxDbiQ8K2I4.
    PRIDEiQ8K2I4.

    PTM databases

    PhosphoSiteiQ8K2I4.

    Expressioni

    Tissue specificityi

    Highest level in liver, high levels in lung, testis, skin and spleen, moderate level in thymus. Activity found in plasma, kidney, liver, spleen, pancreas, brain, testis, epididymis, heart, lung and skeletal muscle.2 Publications

    Gene expression databases

    ArrayExpressiQ8K2I4.
    BgeeiQ8K2I4.
    CleanExiMM_MANBA.
    GenevestigatoriQ8K2I4.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000029814.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K2I4.
    SMRiQ8K2I4. Positions 25-879.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.Sequence Analysis

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3250.
    GeneTreeiENSGT00390000001670.
    HOGENOMiHOG000186862.
    HOVERGENiHBG052404.
    InParanoidiQ8K2I4.
    KOiK01192.
    OrthoDBiEOG7S7SD2.
    PhylomeDBiQ8K2I4.
    TreeFamiTF105723.

    Family and domain databases

    Gene3Di2.60.120.260. 2 hits.
    2.60.40.320. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR008979. Galactose-bd-like.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR028369. Mannanase.
    [Graphical view]
    PANTHERiPTHR10066:SF12. PTHR10066:SF12. 1 hit.
    PfamiPF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 3 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8K2I4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHLHLLLILA LFRAGCVVAG PSYSLSGSWR VSNGNGSLEL PATVPGYVHS    50
    ALHQHGLIQD PYYRFNDLNY RWISLDNWTY STEFKIPFNL SEWQKVKLIF 100
    DGVDTVAEIL FNNVTIGKTD NMFTGYSFDI TNVVKDVNSL KLQFRSAVQY 150
    AECQSKAHTS YRVPPECPPV EQKGECHVNF IRKAQCSFSW DWGPSFPSQG 200
    IWKDVRIEAY NIAHLDYLTF LPVYDNASQA WNIEIKASFD VASSKSVGGQ 250
    VTVAIPQLKT QQTNDIELQQ EQRIVKLLVK IRKDVAVETW WPRGHGNQTG 300
    YNMTILFALD GGLKIEKAAK VYFRTVQLIE EGIKGSPGLS FYFKINGLPI 350
    FLKGSNWIPA DSFQDKVTSD RLQLLFQSVV DANMNTLRVW GGGIYEQDEF 400
    YALCDELGIM VWQDFMFASA LYPTEPGFLA SVRKEVTYQV RRLKSHPSII 450
    IWSGNNENEV ALSVNWFHVN PRDMKTYIDD YVTLYVKNIR KIVLSEDKSR 500
    PFIASSPTNG MKTMEEGWIS YDPYSIQYGD IHFYNYADDC WNWKIFPKAR 550
    LVSEYGYQSW PSFSTLEKVS SQEDWAYNSR FSLHRQHHED GNHQMLHQVK 600
    MHFKLPQGTD PLRTFKDTIY LTQVMQAQCI KTETEFYLRS RSEIVDGKGH 650
    TMGALYWQLN DIWQAPSWAS LEYGGKWKML HYFARRFFAP LLPVGFEDEG 700
    VFYVYGVSDL HKDHHTQLTV RLHHWSSPKP LCSLVNSSIV VKAGEAVVLF 750
    QMPVSELLKR CRGCTRETCV VSFYFSTDKE LFSPTNYHFL SSLKDAKGLL 800
    EANITVNISQ KGNVFVFDLE TSAVAPFVWL DVGSIPGRFS DNGFLMIRKK 850
    LSVLFYPWKP TSKSELQQAF SVTSLTDTY 879
    Length:879
    Mass (Da):100,831
    Last modified:October 1, 2002 - v1
    Checksum:i1DE203C44557719B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti130 – 1301I → V in AAK18177. (PubMed:11892998)Curated
    Sequence conflicti130 – 1301I → V in BAE24954. (PubMed:16141072)Curated
    Sequence conflicti130 – 1301I → V in BAE38715. (PubMed:16141072)Curated
    Sequence conflicti143 – 1453QFR → RFW in BAE38715. (PubMed:16141072)Curated
    Sequence conflicti153 – 1531C → R in BAE38715. (PubMed:16141072)Curated
    Sequence conflicti160 – 1623SYR → RYP in BAE38715. (PubMed:16141072)Curated
    Sequence conflicti236 – 2361K → E in AAK18177. (PubMed:11892998)Curated
    Sequence conflicti236 – 2361K → E in BAE24954. (PubMed:16141072)Curated
    Sequence conflicti236 – 2361K → E in BAE38715. (PubMed:16141072)Curated
    Sequence conflicti236 – 2361K → E in BAE41690. (PubMed:16141072)Curated
    Sequence conflicti248 – 2481G → S in AAK18177. (PubMed:11892998)Curated
    Sequence conflicti248 – 2481G → S in BAE24954. (PubMed:16141072)Curated
    Sequence conflicti264 – 2641N → H in BAE38715. (PubMed:16141072)Curated
    Sequence conflicti376 – 3761F → L in BAE24954. (PubMed:16141072)Curated
    Sequence conflicti491 – 4911K → N in BAE38715. (PubMed:16141072)Curated
    Sequence conflicti511 – 5111M → I in BAE38715. (PubMed:16141072)Curated
    Sequence conflicti714 – 7141H → Y in BAE38715. (PubMed:16141072)Curated
    Sequence conflicti744 – 7441G → R in BAE38715. (PubMed:16141072)Curated
    Sequence conflicti834 – 8341S → G in BAE38715. (PubMed:16141072)Curated
    Sequence conflicti848 – 8481R → Q in BAE38715. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF306557 mRNA. Translation: AAK18177.1.
    AK010623 mRNA. Translation: BAB27069.1.
    AK142165 mRNA. Translation: BAE24954.1.
    AK166340 mRNA. Translation: BAE38715.1.
    AK170290 mRNA. Translation: BAE41690.1.
    BC031409 mRNA. Translation: AAH31409.1.
    CCDSiCCDS17857.1.
    RefSeqiNP_081564.3. NM_027288.3.
    UniGeneiMm.280536.

    Genome annotation databases

    EnsembliENSMUST00000029814; ENSMUSP00000029814; ENSMUSG00000028164.
    GeneIDi110173.
    KEGGimmu:110173.
    UCSCiuc008rlu.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF306557 mRNA. Translation: AAK18177.1 .
    AK010623 mRNA. Translation: BAB27069.1 .
    AK142165 mRNA. Translation: BAE24954.1 .
    AK166340 mRNA. Translation: BAE38715.1 .
    AK170290 mRNA. Translation: BAE41690.1 .
    BC031409 mRNA. Translation: AAH31409.1 .
    CCDSi CCDS17857.1.
    RefSeqi NP_081564.3. NM_027288.3.
    UniGenei Mm.280536.

    3D structure databases

    ProteinModelPortali Q8K2I4.
    SMRi Q8K2I4. Positions 25-879.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000029814.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    PTM databases

    PhosphoSitei Q8K2I4.

    Proteomic databases

    MaxQBi Q8K2I4.
    PaxDbi Q8K2I4.
    PRIDEi Q8K2I4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029814 ; ENSMUSP00000029814 ; ENSMUSG00000028164 .
    GeneIDi 110173.
    KEGGi mmu:110173.
    UCSCi uc008rlu.2. mouse.

    Organism-specific databases

    CTDi 4126.
    MGIi MGI:88175. Manba.

    Phylogenomic databases

    eggNOGi COG3250.
    GeneTreei ENSGT00390000001670.
    HOGENOMi HOG000186862.
    HOVERGENi HBG052404.
    InParanoidi Q8K2I4.
    KOi K01192.
    OrthoDBi EOG7S7SD2.
    PhylomeDBi Q8K2I4.
    TreeFami TF105723.

    Enzyme and pathway databases

    UniPathwayi UPA00280 .

    Miscellaneous databases

    NextBioi 363463.
    PROi Q8K2I4.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8K2I4.
    Bgeei Q8K2I4.
    CleanExi MM_MANBA.
    Genevestigatori Q8K2I4.

    Family and domain databases

    Gene3Di 2.60.120.260. 2 hits.
    2.60.40.320. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR008979. Galactose-bd-like.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR028369. Mannanase.
    [Graphical view ]
    PANTHERi PTHR10066:SF12. PTHR10066:SF12. 1 hit.
    Pfami PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 3 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Mouse beta-mannosidase, cDNA cloning, expression, and chromosomal localization."
      Beccari T., Bibi L., Stinchi S., Stirling J.L., Orlacchio A.
      Biosci. Rep. 21:315-323(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: C57BL/6Imported.
      Tissue: Spleen1 Publication.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6JImported and NODImported.
      Tissue: Dendritic cell, Embryonic heartImported, Embryonic stem cell and Mammary glandImported.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/NImported.
      Tissue: Mammary glandImported.
    4. "Beta-mannosidosis mice: a model for the human lysosomal storage disease."
      Zhu M., Lovell K.L., Patterson J.S., Saunders T.L., Hughes E.D., Friderici K.H.
      Hum. Mol. Genet. 15:493-500(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiMANBA_MOUSE
    AccessioniPrimary (citable) accession number: Q8K2I4
    Secondary accession number(s): Q3TDB3
    , Q3TLS7, Q3UQT5, Q99MS1, Q9CRH3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3