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Q8K2I4

- MANBA_MOUSE

UniProt

Q8K2I4 - MANBA_MOUSE

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Protein

Beta-mannosidase

Gene

Manba

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.By similarity

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei457 – 4571Proton donorBy similarity

GO - Molecular functioni

  1. beta-mannosidase activity Source: MGI
  2. mannose binding Source: Ensembl

GO - Biological processi

  1. glycoprotein catabolic process Source: MGI
  2. mannan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

UniPathwayiUPA00280.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-mannosidase (EC:3.2.1.25)
Alternative name(s):
Lysosomal beta A mannosidase
Mannanase
Short name:
Mannase
Gene namesi
Name:ManbaImported
Synonyms:Bmn1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:88175. Manba.

Subcellular locationi

Lysosome By similarity

GO - Cellular componenti

  1. lysosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Disruption phenotypei

Mice show vacuolation in the central nervous system and accumulation of disaccharides in brain and epididymis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 879860Beta-mannosidaseSequence AnalysisPRO_0000250613Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi226 – 2261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi302 – 3021N-linked (GlcNAc...)Sequence Analysis
Glycosylationi736 – 7361N-linked (GlcNAc...)Sequence Analysis
Glycosylationi803 – 8031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi807 – 8071N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8K2I4.
PaxDbiQ8K2I4.
PRIDEiQ8K2I4.

PTM databases

PhosphoSiteiQ8K2I4.

Expressioni

Tissue specificityi

Highest level in liver, high levels in lung, testis, skin and spleen, moderate level in thymus. Activity found in plasma, kidney, liver, spleen, pancreas, brain, testis, epididymis, heart, lung and skeletal muscle.2 Publications

Gene expression databases

BgeeiQ8K2I4.
CleanExiMM_MANBA.
ExpressionAtlasiQ8K2I4. baseline and differential.
GenevestigatoriQ8K2I4.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029814.

Structurei

3D structure databases

ProteinModelPortaliQ8K2I4.
SMRiQ8K2I4. Positions 25-879.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Sequence Analysis

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3250.
GeneTreeiENSGT00390000001670.
HOGENOMiHOG000186862.
HOVERGENiHBG052404.
InParanoidiQ8K2I4.
KOiK01192.
OrthoDBiEOG7S7SD2.
PhylomeDBiQ8K2I4.
TreeFamiTF105723.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view]
PANTHERiPTHR10066:SF12. PTHR10066:SF12. 1 hit.
PfamiPF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 3 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K2I4 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHLHLLLILA LFRAGCVVAG PSYSLSGSWR VSNGNGSLEL PATVPGYVHS
60 70 80 90 100
ALHQHGLIQD PYYRFNDLNY RWISLDNWTY STEFKIPFNL SEWQKVKLIF
110 120 130 140 150
DGVDTVAEIL FNNVTIGKTD NMFTGYSFDI TNVVKDVNSL KLQFRSAVQY
160 170 180 190 200
AECQSKAHTS YRVPPECPPV EQKGECHVNF IRKAQCSFSW DWGPSFPSQG
210 220 230 240 250
IWKDVRIEAY NIAHLDYLTF LPVYDNASQA WNIEIKASFD VASSKSVGGQ
260 270 280 290 300
VTVAIPQLKT QQTNDIELQQ EQRIVKLLVK IRKDVAVETW WPRGHGNQTG
310 320 330 340 350
YNMTILFALD GGLKIEKAAK VYFRTVQLIE EGIKGSPGLS FYFKINGLPI
360 370 380 390 400
FLKGSNWIPA DSFQDKVTSD RLQLLFQSVV DANMNTLRVW GGGIYEQDEF
410 420 430 440 450
YALCDELGIM VWQDFMFASA LYPTEPGFLA SVRKEVTYQV RRLKSHPSII
460 470 480 490 500
IWSGNNENEV ALSVNWFHVN PRDMKTYIDD YVTLYVKNIR KIVLSEDKSR
510 520 530 540 550
PFIASSPTNG MKTMEEGWIS YDPYSIQYGD IHFYNYADDC WNWKIFPKAR
560 570 580 590 600
LVSEYGYQSW PSFSTLEKVS SQEDWAYNSR FSLHRQHHED GNHQMLHQVK
610 620 630 640 650
MHFKLPQGTD PLRTFKDTIY LTQVMQAQCI KTETEFYLRS RSEIVDGKGH
660 670 680 690 700
TMGALYWQLN DIWQAPSWAS LEYGGKWKML HYFARRFFAP LLPVGFEDEG
710 720 730 740 750
VFYVYGVSDL HKDHHTQLTV RLHHWSSPKP LCSLVNSSIV VKAGEAVVLF
760 770 780 790 800
QMPVSELLKR CRGCTRETCV VSFYFSTDKE LFSPTNYHFL SSLKDAKGLL
810 820 830 840 850
EANITVNISQ KGNVFVFDLE TSAVAPFVWL DVGSIPGRFS DNGFLMIRKK
860 870
LSVLFYPWKP TSKSELQQAF SVTSLTDTY
Length:879
Mass (Da):100,831
Last modified:October 1, 2002 - v1
Checksum:i1DE203C44557719B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti130 – 1301I → V in AAK18177. (PubMed:11892998)Curated
Sequence conflicti130 – 1301I → V in BAE24954. (PubMed:16141072)Curated
Sequence conflicti130 – 1301I → V in BAE38715. (PubMed:16141072)Curated
Sequence conflicti143 – 1453QFR → RFW in BAE38715. (PubMed:16141072)Curated
Sequence conflicti153 – 1531C → R in BAE38715. (PubMed:16141072)Curated
Sequence conflicti160 – 1623SYR → RYP in BAE38715. (PubMed:16141072)Curated
Sequence conflicti236 – 2361K → E in AAK18177. (PubMed:11892998)Curated
Sequence conflicti236 – 2361K → E in BAE24954. (PubMed:16141072)Curated
Sequence conflicti236 – 2361K → E in BAE38715. (PubMed:16141072)Curated
Sequence conflicti236 – 2361K → E in BAE41690. (PubMed:16141072)Curated
Sequence conflicti248 – 2481G → S in AAK18177. (PubMed:11892998)Curated
Sequence conflicti248 – 2481G → S in BAE24954. (PubMed:16141072)Curated
Sequence conflicti264 – 2641N → H in BAE38715. (PubMed:16141072)Curated
Sequence conflicti376 – 3761F → L in BAE24954. (PubMed:16141072)Curated
Sequence conflicti491 – 4911K → N in BAE38715. (PubMed:16141072)Curated
Sequence conflicti511 – 5111M → I in BAE38715. (PubMed:16141072)Curated
Sequence conflicti714 – 7141H → Y in BAE38715. (PubMed:16141072)Curated
Sequence conflicti744 – 7441G → R in BAE38715. (PubMed:16141072)Curated
Sequence conflicti834 – 8341S → G in BAE38715. (PubMed:16141072)Curated
Sequence conflicti848 – 8481R → Q in BAE38715. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF306557 mRNA. Translation: AAK18177.1.
AK010623 mRNA. Translation: BAB27069.1.
AK142165 mRNA. Translation: BAE24954.1.
AK166340 mRNA. Translation: BAE38715.1.
AK170290 mRNA. Translation: BAE41690.1.
BC031409 mRNA. Translation: AAH31409.1.
CCDSiCCDS17857.1.
RefSeqiNP_081564.3. NM_027288.3.
UniGeneiMm.280536.

Genome annotation databases

EnsembliENSMUST00000029814; ENSMUSP00000029814; ENSMUSG00000028164.
GeneIDi110173.
KEGGimmu:110173.
UCSCiuc008rlu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF306557 mRNA. Translation: AAK18177.1 .
AK010623 mRNA. Translation: BAB27069.1 .
AK142165 mRNA. Translation: BAE24954.1 .
AK166340 mRNA. Translation: BAE38715.1 .
AK170290 mRNA. Translation: BAE41690.1 .
BC031409 mRNA. Translation: AAH31409.1 .
CCDSi CCDS17857.1.
RefSeqi NP_081564.3. NM_027288.3.
UniGenei Mm.280536.

3D structure databases

ProteinModelPortali Q8K2I4.
SMRi Q8K2I4. Positions 25-879.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000029814.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

PTM databases

PhosphoSitei Q8K2I4.

Proteomic databases

MaxQBi Q8K2I4.
PaxDbi Q8K2I4.
PRIDEi Q8K2I4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000029814 ; ENSMUSP00000029814 ; ENSMUSG00000028164 .
GeneIDi 110173.
KEGGi mmu:110173.
UCSCi uc008rlu.2. mouse.

Organism-specific databases

CTDi 4126.
MGIi MGI:88175. Manba.

Phylogenomic databases

eggNOGi COG3250.
GeneTreei ENSGT00390000001670.
HOGENOMi HOG000186862.
HOVERGENi HBG052404.
InParanoidi Q8K2I4.
KOi K01192.
OrthoDBi EOG7S7SD2.
PhylomeDBi Q8K2I4.
TreeFami TF105723.

Enzyme and pathway databases

UniPathwayi UPA00280 .

Miscellaneous databases

NextBioi 363463.
PROi Q8K2I4.
SOURCEi Search...

Gene expression databases

Bgeei Q8K2I4.
CleanExi MM_MANBA.
ExpressionAtlasi Q8K2I4. baseline and differential.
Genevestigatori Q8K2I4.

Family and domain databases

Gene3Di 2.60.120.260. 2 hits.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view ]
PANTHERi PTHR10066:SF12. PTHR10066:SF12. 1 hit.
Pfami PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 3 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse beta-mannosidase, cDNA cloning, expression, and chromosomal localization."
    Beccari T., Bibi L., Stinchi S., Stirling J.L., Orlacchio A.
    Biosci. Rep. 21:315-323(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: C57BL/6Imported.
    Tissue: Spleen1 Publication.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6JImported and NODImported.
    Tissue: Dendritic cell, Embryonic heartImported, Embryonic stem cell and Mammary glandImported.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/NImported.
    Tissue: Mammary glandImported.
  4. "Beta-mannosidosis mice: a model for the human lysosomal storage disease."
    Zhu M., Lovell K.L., Patterson J.S., Saunders T.L., Hughes E.D., Friderici K.H.
    Hum. Mol. Genet. 15:493-500(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiMANBA_MOUSE
AccessioniPrimary (citable) accession number: Q8K2I4
Secondary accession number(s): Q3TDB3
, Q3TLS7, Q3UQT5, Q99MS1, Q9CRH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3