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Protein

Beta-mannosidase

Gene

Manba

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.By similarity

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.By similarity

Pathwayi: N-glycan degradation

This protein is involved in the pathway N-glycan degradation, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway N-glycan degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei457Proton donorBy similarity1

GO - Molecular functioni

  • beta-mannosidase activity Source: MGI
  • hydrolase activity Source: MGI
  • mannose binding Source: MGI

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • glycoprotein catabolic process Source: MGI

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

UniPathwayiUPA00280

Protein family/group databases

CAZyiGH2 Glycoside Hydrolase Family 2

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-mannosidase (EC:3.2.1.25)
Alternative name(s):
Lysosomal beta A mannosidase
Mannanase
Short name:
Mannase
Gene namesi
Name:ManbaImported
Synonyms:Bmn1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:88175 Manba

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Disruption phenotypei

Mice show vacuolation in the central nervous system and accumulation of disaccharides in brain and epididymis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000025061320 – 879Beta-mannosidaseSequence analysisAdd BLAST860

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi35N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi77N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi89N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi113N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi226N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi297N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi302N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi736N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi803N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi807N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ8K2I4
MaxQBiQ8K2I4
PaxDbiQ8K2I4
PeptideAtlasiQ8K2I4
PRIDEiQ8K2I4

PTM databases

iPTMnetiQ8K2I4
PhosphoSitePlusiQ8K2I4

Expressioni

Tissue specificityi

Highest level in liver, high levels in lung, testis, skin and spleen, moderate level in thymus. Activity found in plasma, kidney, liver, spleen, pancreas, brain, testis, epididymis, heart, lung and skeletal muscle.2 Publications

Gene expression databases

CleanExiMM_MANBA

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029814

Structurei

3D structure databases

ProteinModelPortaliQ8K2I4
SMRiQ8K2I4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Sequence analysis

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2230 Eukaryota
COG3250 LUCA
HOGENOMiHOG000186862
HOVERGENiHBG052404
InParanoidiQ8K2I4
KOiK01192
PhylomeDBiQ8K2I4
TreeFamiTF105723

Family and domain databases

Gene3Di2.60.120.260, 1 hit
2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR036156 Beta-gal/glucu_dom_sf
IPR008979 Galactose-bd-like_sf
IPR006103 Glyco_hydro_2_cat
IPR006104 Glyco_hydro_2_N
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF02836 Glyco_hydro_2_C, 1 hit
PF02837 Glyco_hydro_2_N, 1 hit
SUPFAMiSSF49303 SSF49303, 3 hits
SSF49785 SSF49785, 1 hit
SSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K2I4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLHLLLILA LFRAGCVVAG PSYSLSGSWR VSNGNGSLEL PATVPGYVHS
60 70 80 90 100
ALHQHGLIQD PYYRFNDLNY RWISLDNWTY STEFKIPFNL SEWQKVKLIF
110 120 130 140 150
DGVDTVAEIL FNNVTIGKTD NMFTGYSFDI TNVVKDVNSL KLQFRSAVQY
160 170 180 190 200
AECQSKAHTS YRVPPECPPV EQKGECHVNF IRKAQCSFSW DWGPSFPSQG
210 220 230 240 250
IWKDVRIEAY NIAHLDYLTF LPVYDNASQA WNIEIKASFD VASSKSVGGQ
260 270 280 290 300
VTVAIPQLKT QQTNDIELQQ EQRIVKLLVK IRKDVAVETW WPRGHGNQTG
310 320 330 340 350
YNMTILFALD GGLKIEKAAK VYFRTVQLIE EGIKGSPGLS FYFKINGLPI
360 370 380 390 400
FLKGSNWIPA DSFQDKVTSD RLQLLFQSVV DANMNTLRVW GGGIYEQDEF
410 420 430 440 450
YALCDELGIM VWQDFMFASA LYPTEPGFLA SVRKEVTYQV RRLKSHPSII
460 470 480 490 500
IWSGNNENEV ALSVNWFHVN PRDMKTYIDD YVTLYVKNIR KIVLSEDKSR
510 520 530 540 550
PFIASSPTNG MKTMEEGWIS YDPYSIQYGD IHFYNYADDC WNWKIFPKAR
560 570 580 590 600
LVSEYGYQSW PSFSTLEKVS SQEDWAYNSR FSLHRQHHED GNHQMLHQVK
610 620 630 640 650
MHFKLPQGTD PLRTFKDTIY LTQVMQAQCI KTETEFYLRS RSEIVDGKGH
660 670 680 690 700
TMGALYWQLN DIWQAPSWAS LEYGGKWKML HYFARRFFAP LLPVGFEDEG
710 720 730 740 750
VFYVYGVSDL HKDHHTQLTV RLHHWSSPKP LCSLVNSSIV VKAGEAVVLF
760 770 780 790 800
QMPVSELLKR CRGCTRETCV VSFYFSTDKE LFSPTNYHFL SSLKDAKGLL
810 820 830 840 850
EANITVNISQ KGNVFVFDLE TSAVAPFVWL DVGSIPGRFS DNGFLMIRKK
860 870
LSVLFYPWKP TSKSELQQAF SVTSLTDTY
Length:879
Mass (Da):100,831
Last modified:October 1, 2002 - v1
Checksum:i1DE203C44557719B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti130I → V in AAK18177 (PubMed:11892998).Curated1
Sequence conflicti130I → V in BAE24954 (PubMed:16141072).Curated1
Sequence conflicti130I → V in BAE38715 (PubMed:16141072).Curated1
Sequence conflicti143 – 145QFR → RFW in BAE38715 (PubMed:16141072).Curated3
Sequence conflicti153C → R in BAE38715 (PubMed:16141072).Curated1
Sequence conflicti160 – 162SYR → RYP in BAE38715 (PubMed:16141072).Curated3
Sequence conflicti236K → E in AAK18177 (PubMed:11892998).Curated1
Sequence conflicti236K → E in BAE24954 (PubMed:16141072).Curated1
Sequence conflicti236K → E in BAE38715 (PubMed:16141072).Curated1
Sequence conflicti236K → E in BAE41690 (PubMed:16141072).Curated1
Sequence conflicti248G → S in AAK18177 (PubMed:11892998).Curated1
Sequence conflicti248G → S in BAE24954 (PubMed:16141072).Curated1
Sequence conflicti264N → H in BAE38715 (PubMed:16141072).Curated1
Sequence conflicti376F → L in BAE24954 (PubMed:16141072).Curated1
Sequence conflicti491K → N in BAE38715 (PubMed:16141072).Curated1
Sequence conflicti511M → I in BAE38715 (PubMed:16141072).Curated1
Sequence conflicti714H → Y in BAE38715 (PubMed:16141072).Curated1
Sequence conflicti744G → R in BAE38715 (PubMed:16141072).Curated1
Sequence conflicti834S → G in BAE38715 (PubMed:16141072).Curated1
Sequence conflicti848R → Q in BAE38715 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF306557 mRNA Translation: AAK18177.1
AK010623 mRNA Translation: BAB27069.1
AK142165 mRNA Translation: BAE24954.1
AK166340 mRNA Translation: BAE38715.1
AK170290 mRNA Translation: BAE41690.1
BC031409 mRNA Translation: AAH31409.1
CCDSiCCDS17857.1
RefSeqiNP_081564.3, NM_027288.3
UniGeneiMm.280536

Genome annotation databases

GeneIDi110173
KEGGimmu:110173
UCSCiuc008rlu.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMANBA_MOUSE
AccessioniPrimary (citable) accession number: Q8K2I4
Secondary accession number(s): Q3TDB3
, Q3TLS7, Q3UQT5, Q99MS1, Q9CRH3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 1, 2002
Last modified: June 20, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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