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Q8K2I3 (FMO2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dimethylaniline monooxygenase [N-oxide-forming] 2

EC=1.14.13.8
Alternative name(s):
Dimethylaniline oxidase 2
Pulmonary flavin-containing monooxygenase 2
Short name=FMO 2
Gene names
Name:Fmo2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length535 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. Shows catalytic activity towards methimazole, thiourea, trimethylamine, and the insecticide phorate.

Catalytic activity

N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.

Cofactor

FAD By similarity.

Magnesium By similarity.

Subcellular location

Microsome membrane By similarity. Endoplasmic reticulum membrane By similarity.

Sequence similarities

Belongs to the FMO family.

Biophysicochemical properties

pH dependence:

Optimum pH is 10.5.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 535534Dimethylaniline monooxygenase [N-oxide-forming] 2
PRO_0000147648

Regions

Nucleotide binding9 – 146FAD Potential
Nucleotide binding191 – 1966NADP Potential

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Cross-link492Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1) By similarity

Experimental info

Sequence conflict1691Q → R in AAD56413. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8K2I3 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 705FAD9D8EADB1B1

FASTA53560,974
        10         20         30         40         50         60 
MAKKVVVIGA GVSGLISLKC CVDEGLEPTC FERTEDIGGL WRFKENVEDG RASIYRSVIT 

        70         80         90        100        110        120 
NTSKEMSCFS DFPMPEDFPN FLHNSKLLEY FRIFAKKFDL LKYIQFQTTV ISVKKRPDFA 

       130        140        150        160        170        180 
SSGQWEVYTQ SNGKEQRTVF DAVMVCSGHH IQPHLPLKSF PGIERFRGQY FHSREYKHPV 

       190        200        210        220        230        240 
GFEGKRILVV GIGNSAADIA SELSKTAAQV FVSTRHGSWV MSRISEDGYP WDMVFHTRFS 

       250        260        270        280        290        300 
SMLRNVLPRT VVKWMMEQQM NRWFNHENYG LVPQNKYLMK EPVLNDDLPS RLLYGAIKVK 

       310        320        330        340        350        360 
TRVKELTETA VVFEDGTVEE DVDIIVFATG YTFSFSFLED SLVKVEDNRV SLYKAMFPPH 

       370        380        390        400        410        420 
LEKPTLACIG LIQPLGSIFP TVELQARWAT RVFKGLCSLP SETTMMADIV ERNEKRVNLF 

       430        440        450        460        470        480 
GKSQSQILQT NYVDYLDELA LEIGAKPDFV SLFFKDPKLA VKLYFGPCNS YQYRLVGPGQ 

       490        500        510        520        530 
WEGARNAILT QKQRILKPLK TRTLQSSDSA PVSFLLKILG LLAVVLAFFF QLQGF 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing, expression, and characterization of cDNA expressed flavin-containing monooxygenase 2 from mouse."
Karoly E.D., Rose R.L.
J. Biochem. Mol. Toxicol. 15:300-308(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
Strain: C57BL/6.
Tissue: Kidney.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF184981 mRNA. Translation: AAD56413.1.
BC031415 mRNA. Translation: AAH31415.1.
CCDSCCDS15425.1.
RefSeqNP_061369.2. NM_018881.3.
XP_006497012.1. XM_006496949.1.
UniGeneMm.10929.

3D structure databases

ProteinModelPortalQ8K2I3.
SMRQ8K2I3. Positions 4-215, 307-439.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8K2I3. 1 interaction.
MINTMINT-4117353.
STRING10090.ENSMUSP00000107135.

PTM databases

PhosphoSiteQ8K2I3.

Proteomic databases

MaxQBQ8K2I3.
PaxDbQ8K2I3.
PRIDEQ8K2I3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000045902; ENSMUSP00000044405; ENSMUSG00000040170.
ENSMUST00000111510; ENSMUSP00000107135; ENSMUSG00000040170.
GeneID55990.
KEGGmmu:55990.
UCSCuc007dgz.1. mouse.

Organism-specific databases

CTD2327.
MGIMGI:1916776. Fmo2.

Phylogenomic databases

eggNOGCOG2072.
GeneTreeENSGT00740000115132.
HOGENOMHOG000076537.
HOVERGENHBG002037.
InParanoidQ8K2I3.
KOK00485.
OMAHPDGFEG.
OrthoDBEOG7GXPB6.
PhylomeDBQ8K2I3.
TreeFamTF105285.

Gene expression databases

ArrayExpressQ8K2I3.
BgeeQ8K2I3.
CleanExMM_FMO2.
GenevestigatorQ8K2I3.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR012143. DiMe-aniline_mOase.
IPR000960. Flavin_mOase.
IPR020946. Flavin_mOase-like.
IPR002254. Flavin_mOase_2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00743. FMO-like. 1 hit.
[Graphical view]
PIRSFPIRSF000332. FMO. 1 hit.
PRINTSPR00370. FMOXYGENASE.
PR01122. FMOXYGENASE2.
ProtoNetSearch...

Other

ChiTaRSFMO2. mouse.
NextBio311714.
PROQ8K2I3.
SOURCESearch...

Entry information

Entry nameFMO2_MOUSE
AccessionPrimary (citable) accession number: Q8K2I3
Secondary accession number(s): Q9QZF7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot