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Q8K2I3

- FMO2_MOUSE

UniProt

Q8K2I3 - FMO2_MOUSE

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Protein
Dimethylaniline monooxygenase [N-oxide-forming] 2
Gene
Fmo2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. Shows catalytic activity towards methimazole, thiourea, trimethylamine, and the insecticide phorate.

Catalytic activityi

N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.

Cofactori

FAD By similarity.
Magnesium By similarity.

pH dependencei

Optimum pH is 10.5.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FAD Reviewed prediction
Nucleotide bindingi191 – 1966NADP Reviewed prediction

GO - Molecular functioni

  1. N,N-dimethylaniline monooxygenase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro
  3. flavin adenine dinucleotide binding Source: InterPro
  4. monooxygenase activity Source: MGI

GO - Biological processi

  1. NADPH oxidation Source: UniProtKB
  2. drug metabolic process Source: Ensembl
  3. organic acid metabolic process Source: Ensembl
  4. oxygen metabolic process Source: MGI
  5. toxin metabolic process Source: Ensembl
  6. xenobiotic metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, Magnesium, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethylaniline monooxygenase [N-oxide-forming] 2 (EC:1.14.13.8)
Alternative name(s):
Dimethylaniline oxidase 2
Pulmonary flavin-containing monooxygenase 2
Short name:
FMO 2
Gene namesi
Name:Fmo2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1916776. Fmo2.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 535534Dimethylaniline monooxygenase [N-oxide-forming] 2
PRO_0000147648Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Cross-linki492 – 492Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1) By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ8K2I3.
PaxDbiQ8K2I3.
PRIDEiQ8K2I3.

PTM databases

PhosphoSiteiQ8K2I3.

Expressioni

Gene expression databases

ArrayExpressiQ8K2I3.
BgeeiQ8K2I3.
CleanExiMM_FMO2.
GenevestigatoriQ8K2I3.

Interactioni

Protein-protein interaction databases

IntActiQ8K2I3. 1 interaction.
MINTiMINT-4117353.
STRINGi10090.ENSMUSP00000107135.

Structurei

3D structure databases

ProteinModelPortaliQ8K2I3.
SMRiQ8K2I3. Positions 4-215, 307-439.

Family & Domainsi

Sequence similaritiesi

Belongs to the FMO family.

Keywords - Domaini

Transmembrane

Phylogenomic databases

eggNOGiCOG2072.
GeneTreeiENSGT00740000115132.
HOGENOMiHOG000076537.
HOVERGENiHBG002037.
InParanoidiQ8K2I3.
KOiK00485.
OMAiHPDGFEG.
OrthoDBiEOG7GXPB6.
PhylomeDBiQ8K2I3.
TreeFamiTF105285.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR012143. DiMe-aniline_mOase.
IPR000960. Flavin_mOase.
IPR020946. Flavin_mOase-like.
IPR002254. Flavin_mOase_2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00743. FMO-like. 1 hit.
[Graphical view]
PIRSFiPIRSF000332. FMO. 1 hit.
PRINTSiPR00370. FMOXYGENASE.
PR01122. FMOXYGENASE2.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K2I3-1 [UniParc]FASTAAdd to Basket

« Hide

MAKKVVVIGA GVSGLISLKC CVDEGLEPTC FERTEDIGGL WRFKENVEDG    50
RASIYRSVIT NTSKEMSCFS DFPMPEDFPN FLHNSKLLEY FRIFAKKFDL 100
LKYIQFQTTV ISVKKRPDFA SSGQWEVYTQ SNGKEQRTVF DAVMVCSGHH 150
IQPHLPLKSF PGIERFRGQY FHSREYKHPV GFEGKRILVV GIGNSAADIA 200
SELSKTAAQV FVSTRHGSWV MSRISEDGYP WDMVFHTRFS SMLRNVLPRT 250
VVKWMMEQQM NRWFNHENYG LVPQNKYLMK EPVLNDDLPS RLLYGAIKVK 300
TRVKELTETA VVFEDGTVEE DVDIIVFATG YTFSFSFLED SLVKVEDNRV 350
SLYKAMFPPH LEKPTLACIG LIQPLGSIFP TVELQARWAT RVFKGLCSLP 400
SETTMMADIV ERNEKRVNLF GKSQSQILQT NYVDYLDELA LEIGAKPDFV 450
SLFFKDPKLA VKLYFGPCNS YQYRLVGPGQ WEGARNAILT QKQRILKPLK 500
TRTLQSSDSA PVSFLLKILG LLAVVLAFFF QLQGF 535
Length:535
Mass (Da):60,974
Last modified:January 23, 2007 - v3
Checksum:i705FAD9D8EADB1B1
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti169 – 1691Q → R in AAD56413. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF184981 mRNA. Translation: AAD56413.1.
BC031415 mRNA. Translation: AAH31415.1.
CCDSiCCDS15425.1.
RefSeqiNP_061369.2. NM_018881.3.
XP_006497012.1. XM_006496949.1.
UniGeneiMm.10929.

Genome annotation databases

EnsembliENSMUST00000045902; ENSMUSP00000044405; ENSMUSG00000040170.
ENSMUST00000111510; ENSMUSP00000107135; ENSMUSG00000040170.
GeneIDi55990.
KEGGimmu:55990.
UCSCiuc007dgz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF184981 mRNA. Translation: AAD56413.1 .
BC031415 mRNA. Translation: AAH31415.1 .
CCDSi CCDS15425.1.
RefSeqi NP_061369.2. NM_018881.3.
XP_006497012.1. XM_006496949.1.
UniGenei Mm.10929.

3D structure databases

ProteinModelPortali Q8K2I3.
SMRi Q8K2I3. Positions 4-215, 307-439.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8K2I3. 1 interaction.
MINTi MINT-4117353.
STRINGi 10090.ENSMUSP00000107135.

PTM databases

PhosphoSitei Q8K2I3.

Proteomic databases

MaxQBi Q8K2I3.
PaxDbi Q8K2I3.
PRIDEi Q8K2I3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000045902 ; ENSMUSP00000044405 ; ENSMUSG00000040170 .
ENSMUST00000111510 ; ENSMUSP00000107135 ; ENSMUSG00000040170 .
GeneIDi 55990.
KEGGi mmu:55990.
UCSCi uc007dgz.1. mouse.

Organism-specific databases

CTDi 2327.
MGIi MGI:1916776. Fmo2.

Phylogenomic databases

eggNOGi COG2072.
GeneTreei ENSGT00740000115132.
HOGENOMi HOG000076537.
HOVERGENi HBG002037.
InParanoidi Q8K2I3.
KOi K00485.
OMAi HPDGFEG.
OrthoDBi EOG7GXPB6.
PhylomeDBi Q8K2I3.
TreeFami TF105285.

Miscellaneous databases

ChiTaRSi FMO2. mouse.
NextBioi 311714.
PROi Q8K2I3.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8K2I3.
Bgeei Q8K2I3.
CleanExi MM_FMO2.
Genevestigatori Q8K2I3.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR012143. DiMe-aniline_mOase.
IPR000960. Flavin_mOase.
IPR020946. Flavin_mOase-like.
IPR002254. Flavin_mOase_2.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00743. FMO-like. 1 hit.
[Graphical view ]
PIRSFi PIRSF000332. FMO. 1 hit.
PRINTSi PR00370. FMOXYGENASE.
PR01122. FMOXYGENASE2.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing, expression, and characterization of cDNA expressed flavin-containing monooxygenase 2 from mouse."
    Karoly E.D., Rose R.L.
    J. Biochem. Mol. Toxicol. 15:300-308(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Strain: C57BL/6.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiFMO2_MOUSE
AccessioniPrimary (citable) accession number: Q8K2I3
Secondary accession number(s): Q9QZF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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