Reviewed, UniProtKB/Swiss-Prot Q8K2I3 (FMO2_MOUSE)

Names and origin

Protein names

Recommended name:
    Dimethylaniline monooxygenase [N-oxide-forming] 2
    EC=1.14.13.8
Alternative name(s):
    Pulmonary flavin-containing monooxygenase 2
      Short name=FMO 2
    Dimethylaniline oxidase 2

Gene names

Name:

Fmo2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. Shows catalytic activity towards methimazole, thiourea, trimethylamine, and the insecticide phorate.
Catalytic activity	N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.
Cofactor	FAD By similarity. Magnesium By similarity.
Subcellular location	Microsome membrane By similarity. Endoplasmic reticulum membrane By similarity.
Sequence similarities	Belongs to the FMO family.
biophysicochemical properties	pH dependence: Optimum pH is 10.5.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	FAD Flavoprotein Magnesium NADP
Molecular function	Monooxygenase Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxygen and reactive oxygen species metabolic process Ref.1 Inferred from direct assay. Source: MGI
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro flavin-containing monooxygenase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 535	534	Dimethylaniline monooxygenase [N-oxide-forming] 2		PRO_0000147648
Regions
	Nucleotide binding	9 – 14	6	FAD Potential
	Nucleotide binding	191 – 196	6	NADP Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
Experimental info
	Sequence conflict	169	1	Q → R in AAD56413. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8K2I3-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 705FAD9D8EADB1B1	FASTA	535	60,974
	10 20 30 40 50 60 MAKKVVVIGA GVSGLISLKC CVDEGLEPTC FERTEDIGGL WRFKENVEDG RASIYRSVIT 70 80 90 100 110 120 NTSKEMSCFS DFPMPEDFPN FLHNSKLLEY FRIFAKKFDL LKYIQFQTTV ISVKKRPDFA 130 140 150 160 170 180 SSGQWEVYTQ SNGKEQRTVF DAVMVCSGHH IQPHLPLKSF PGIERFRGQY FHSREYKHPV 190 200 210 220 230 240 GFEGKRILVV GIGNSAADIA SELSKTAAQV FVSTRHGSWV MSRISEDGYP WDMVFHTRFS 250 260 270 280 290 300 SMLRNVLPRT VVKWMMEQQM NRWFNHENYG LVPQNKYLMK EPVLNDDLPS RLLYGAIKVK 310 320 330 340 350 360 TRVKELTETA VVFEDGTVEE DVDIIVFATG YTFSFSFLED SLVKVEDNRV SLYKAMFPPH 370 380 390 400 410 420 LEKPTLACIG LIQPLGSIFP TVELQARWAT RVFKGLCSLP SETTMMADIV ERNEKRVNLF 430 440 450 460 470 480 GKSQSQILQT NYVDYLDELA LEIGAKPDFV SLFFKDPKLA VKLYFGPCNS YQYRLVGPGQ 490 500 510 520 530 WEGARNAILT QKQRILKPLK TRTLQSSDSA PVSFLLKILG LLAVVLAFFF QLQGF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing, expression, and characterization of cDNA expressed flavin-containing monooxygenase 2 from mouse." Karoly E.D., Rose R.L. J. Biochem. Mol. Toxicol. 15:300-308(2001) [PubMed: 11835629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Strain: C57BL/6. Tissue: Kidney.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF184981 mRNA. Translation: AAD56413.1. BC031415 mRNA. Translation: AAH31415.1.
IPI	IPI00322245.
RefSeq	NP_061369.2.
UniGene	Mm.10929
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000040170. Mus musculus. [Contig view]
GeneID	55990.
KEGG	mmu:55990.
Organism-specific databases
MGI	MGI:1916776. Fmo2.
Phylogenomic databases
HOGENOM	Q8K2I3.
HOVERGEN	Q8K2I3.
OMA	Q8K2I3. NYVDYLD.
Enzyme and pathway databases
BRENDA	1.14.13.8. 244.
Gene expression databases
ArrayExpress	Q8K2I3.
Bgee	Q8K2I3.
CleanEx	MM_FMO2.
GermOnline	ENSMUSG00000040170. Mus musculus.
Family and domain databases
InterPro	IPR012143. dManiline_mOase. IPR000960. Flavin_mOase. IPR002254. Flavin_mOase_2. [Graphical view]
Pfam	PF00743. FMO-like. 1 hit. [Graphical view]
PIRSF	PIRSF000332. FMO. 1 hit.
PRINTS	PR00370. FMOXYGENASE. PR01122. FMOXYGENASE2.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other Resources
NextBio	311714.
SOURCE	Search...

Entry information

Entry name

FMO2_MOUSE

Accession

Primary (citable) accession number: Q8K2I3
Secondary accession number(s): Q9QZF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 56 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)