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Protein

Dimethylaniline monooxygenase [N-oxide-forming] 2

Gene

Fmo2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. Shows catalytic activity towards methimazole, thiourea, trimethylamine, and the insecticide phorate.

Catalytic activityi

N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.

Cofactori

Protein has several cofactor binding sites:

pH dependencei

Optimum pH is 10.5.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 13FADBy similarity5
Nucleotide bindingi40 – 41FADBy similarity2
Nucleotide bindingi60 – 61NADPBy similarity2
Nucleotide bindingi61 – 62FADBy similarity2
Nucleotide bindingi195 – 198NADPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandFAD, Flavoprotein, Magnesium, NADP

Enzyme and pathway databases

BRENDAi1.14.13.8 3474
ReactomeiR-MMU-217271 FMO oxidises nucleophiles

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethylaniline monooxygenase [N-oxide-forming] 2 (EC:1.14.13.8)
Alternative name(s):
Dimethylaniline oxidase 2
Pulmonary flavin-containing monooxygenase 2
Short name:
FMO 2
Gene namesi
Name:Fmo2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1916776 Fmo2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei510 – 530HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001476482 – 535Dimethylaniline monooxygenase [N-oxide-forming] 2Add BLAST534

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8K2I3
PaxDbiQ8K2I3
PeptideAtlasiQ8K2I3
PRIDEiQ8K2I3

PTM databases

iPTMnetiQ8K2I3
PhosphoSitePlusiQ8K2I3

Expressioni

Gene expression databases

BgeeiENSMUSG00000040170
CleanExiMM_FMO2
ExpressionAtlasiQ8K2I3 baseline and differential
GenevisibleiQ8K2I3 MM

Interactioni

Protein-protein interaction databases

IntActiQ8K2I3, 1 interactor
MINTiQ8K2I3
STRINGi10090.ENSMUSP00000044405

Structurei

3D structure databases

ProteinModelPortaliQ8K2I3
SMRiQ8K2I3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FMO family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1399 Eukaryota
COG2072 LUCA
GeneTreeiENSGT00760000119232
HOGENOMiHOG000076537
HOVERGENiHBG002037
InParanoidiQ8K2I3
KOiK00485
OMAiPQNKYLM
OrthoDBiEOG091G0465
PhylomeDBiQ8K2I3
TreeFamiTF105285

Family and domain databases

Gene3Di3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR000960 Flavin_mOase
IPR020946 Flavin_mOase-like
IPR002254 Flavin_mOase_2
PfamiView protein in Pfam
PF00743 FMO-like, 1 hit
PIRSFiPIRSF000332 FMO, 1 hit
PRINTSiPR00370 FMOXYGENASE
PR01122 FMOXYGENASE2
SUPFAMiSSF51905 SSF51905, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K2I3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKVVVIGA GVSGLISLKC CVDEGLEPTC FERTEDIGGL WRFKENVEDG
60 70 80 90 100
RASIYRSVIT NTSKEMSCFS DFPMPEDFPN FLHNSKLLEY FRIFAKKFDL
110 120 130 140 150
LKYIQFQTTV ISVKKRPDFA SSGQWEVYTQ SNGKEQRTVF DAVMVCSGHH
160 170 180 190 200
IQPHLPLKSF PGIERFRGQY FHSREYKHPV GFEGKRILVV GIGNSAADIA
210 220 230 240 250
SELSKTAAQV FVSTRHGSWV MSRISEDGYP WDMVFHTRFS SMLRNVLPRT
260 270 280 290 300
VVKWMMEQQM NRWFNHENYG LVPQNKYLMK EPVLNDDLPS RLLYGAIKVK
310 320 330 340 350
TRVKELTETA VVFEDGTVEE DVDIIVFATG YTFSFSFLED SLVKVEDNRV
360 370 380 390 400
SLYKAMFPPH LEKPTLACIG LIQPLGSIFP TVELQARWAT RVFKGLCSLP
410 420 430 440 450
SETTMMADIV ERNEKRVNLF GKSQSQILQT NYVDYLDELA LEIGAKPDFV
460 470 480 490 500
SLFFKDPKLA VKLYFGPCNS YQYRLVGPGQ WEGARNAILT QKQRILKPLK
510 520 530
TRTLQSSDSA PVSFLLKILG LLAVVLAFFF QLQGF
Length:535
Mass (Da):60,974
Last modified:January 23, 2007 - v3
Checksum:i705FAD9D8EADB1B1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti169Q → R in AAD56413 (PubMed:11835629).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF184981 mRNA Translation: AAD56413.1
BC031415 mRNA Translation: AAH31415.1
CCDSiCCDS15425.1
RefSeqiNP_061369.2, NM_018881.3
XP_006497012.1, XM_006496949.3
UniGeneiMm.10929

Genome annotation databases

EnsembliENSMUST00000045902; ENSMUSP00000044405; ENSMUSG00000040170
ENSMUST00000111510; ENSMUSP00000107135; ENSMUSG00000040170
GeneIDi55990
KEGGimmu:55990
UCSCiuc007dgz.1 mouse

Similar proteinsi

Entry informationi

Entry nameiFMO2_MOUSE
AccessioniPrimary (citable) accession number: Q8K2I3
Secondary accession number(s): Q9QZF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 134 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health