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Q8K2I3

- FMO2_MOUSE

UniProt

Q8K2I3 - FMO2_MOUSE

Protein

Dimethylaniline monooxygenase [N-oxide-forming] 2

Gene

Fmo2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. Shows catalytic activity towards methimazole, thiourea, trimethylamine, and the insecticide phorate.

    Catalytic activityi

    N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.

    Cofactori

    FAD.By similarity
    Magnesium.By similarity

    pH dependencei

    Optimum pH is 10.5.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi9 – 146FADSequence Analysis
    Nucleotide bindingi191 – 1966NADPSequence Analysis

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. monooxygenase activity Source: MGI
    3. N,N-dimethylaniline monooxygenase activity Source: UniProtKB-EC
    4. NADP binding Source: InterPro

    GO - Biological processi

    1. drug metabolic process Source: Ensembl
    2. NADPH oxidation Source: UniProtKB
    3. organic acid metabolic process Source: Ensembl
    4. oxygen metabolic process Source: MGI
    5. toxin metabolic process Source: Ensembl
    6. xenobiotic metabolic process Source: Ensembl

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, Magnesium, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dimethylaniline monooxygenase [N-oxide-forming] 2 (EC:1.14.13.8)
    Alternative name(s):
    Dimethylaniline oxidase 2
    Pulmonary flavin-containing monooxygenase 2
    Short name:
    FMO 2
    Gene namesi
    Name:Fmo2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1916776. Fmo2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 535534Dimethylaniline monooxygenase [N-oxide-forming] 2PRO_0000147648Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Cross-linki492 – 492Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1)By similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Ubl conjugation

    Proteomic databases

    MaxQBiQ8K2I3.
    PaxDbiQ8K2I3.
    PRIDEiQ8K2I3.

    PTM databases

    PhosphoSiteiQ8K2I3.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8K2I3.
    BgeeiQ8K2I3.
    CleanExiMM_FMO2.
    GenevestigatoriQ8K2I3.

    Interactioni

    Protein-protein interaction databases

    IntActiQ8K2I3. 1 interaction.
    MINTiMINT-4117353.
    STRINGi10090.ENSMUSP00000107135.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K2I3.
    SMRiQ8K2I3. Positions 4-215, 307-439.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FMO family.Curated

    Keywords - Domaini

    Transmembrane

    Phylogenomic databases

    eggNOGiCOG2072.
    GeneTreeiENSGT00740000115132.
    HOGENOMiHOG000076537.
    HOVERGENiHBG002037.
    InParanoidiQ8K2I3.
    KOiK00485.
    OMAiHPDGFEG.
    OrthoDBiEOG7GXPB6.
    PhylomeDBiQ8K2I3.
    TreeFamiTF105285.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR012143. DiMe-aniline_mOase.
    IPR000960. Flavin_mOase.
    IPR020946. Flavin_mOase-like.
    IPR002254. Flavin_mOase_2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00743. FMO-like. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000332. FMO. 1 hit.
    PRINTSiPR00370. FMOXYGENASE.
    PR01122. FMOXYGENASE2.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8K2I3-1 [UniParc]FASTAAdd to Basket

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    MAKKVVVIGA GVSGLISLKC CVDEGLEPTC FERTEDIGGL WRFKENVEDG    50
    RASIYRSVIT NTSKEMSCFS DFPMPEDFPN FLHNSKLLEY FRIFAKKFDL 100
    LKYIQFQTTV ISVKKRPDFA SSGQWEVYTQ SNGKEQRTVF DAVMVCSGHH 150
    IQPHLPLKSF PGIERFRGQY FHSREYKHPV GFEGKRILVV GIGNSAADIA 200
    SELSKTAAQV FVSTRHGSWV MSRISEDGYP WDMVFHTRFS SMLRNVLPRT 250
    VVKWMMEQQM NRWFNHENYG LVPQNKYLMK EPVLNDDLPS RLLYGAIKVK 300
    TRVKELTETA VVFEDGTVEE DVDIIVFATG YTFSFSFLED SLVKVEDNRV 350
    SLYKAMFPPH LEKPTLACIG LIQPLGSIFP TVELQARWAT RVFKGLCSLP 400
    SETTMMADIV ERNEKRVNLF GKSQSQILQT NYVDYLDELA LEIGAKPDFV 450
    SLFFKDPKLA VKLYFGPCNS YQYRLVGPGQ WEGARNAILT QKQRILKPLK 500
    TRTLQSSDSA PVSFLLKILG LLAVVLAFFF QLQGF 535
    Length:535
    Mass (Da):60,974
    Last modified:January 23, 2007 - v3
    Checksum:i705FAD9D8EADB1B1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti169 – 1691Q → R in AAD56413. (PubMed:11835629)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF184981 mRNA. Translation: AAD56413.1.
    BC031415 mRNA. Translation: AAH31415.1.
    CCDSiCCDS15425.1.
    RefSeqiNP_061369.2. NM_018881.3.
    XP_006497012.1. XM_006496949.1.
    UniGeneiMm.10929.

    Genome annotation databases

    EnsembliENSMUST00000045902; ENSMUSP00000044405; ENSMUSG00000040170.
    ENSMUST00000111510; ENSMUSP00000107135; ENSMUSG00000040170.
    GeneIDi55990.
    KEGGimmu:55990.
    UCSCiuc007dgz.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF184981 mRNA. Translation: AAD56413.1 .
    BC031415 mRNA. Translation: AAH31415.1 .
    CCDSi CCDS15425.1.
    RefSeqi NP_061369.2. NM_018881.3.
    XP_006497012.1. XM_006496949.1.
    UniGenei Mm.10929.

    3D structure databases

    ProteinModelPortali Q8K2I3.
    SMRi Q8K2I3. Positions 4-215, 307-439.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8K2I3. 1 interaction.
    MINTi MINT-4117353.
    STRINGi 10090.ENSMUSP00000107135.

    PTM databases

    PhosphoSitei Q8K2I3.

    Proteomic databases

    MaxQBi Q8K2I3.
    PaxDbi Q8K2I3.
    PRIDEi Q8K2I3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000045902 ; ENSMUSP00000044405 ; ENSMUSG00000040170 .
    ENSMUST00000111510 ; ENSMUSP00000107135 ; ENSMUSG00000040170 .
    GeneIDi 55990.
    KEGGi mmu:55990.
    UCSCi uc007dgz.1. mouse.

    Organism-specific databases

    CTDi 2327.
    MGIi MGI:1916776. Fmo2.

    Phylogenomic databases

    eggNOGi COG2072.
    GeneTreei ENSGT00740000115132.
    HOGENOMi HOG000076537.
    HOVERGENi HBG002037.
    InParanoidi Q8K2I3.
    KOi K00485.
    OMAi HPDGFEG.
    OrthoDBi EOG7GXPB6.
    PhylomeDBi Q8K2I3.
    TreeFami TF105285.

    Miscellaneous databases

    ChiTaRSi FMO2. mouse.
    NextBioi 311714.
    PROi Q8K2I3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8K2I3.
    Bgeei Q8K2I3.
    CleanExi MM_FMO2.
    Genevestigatori Q8K2I3.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR012143. DiMe-aniline_mOase.
    IPR000960. Flavin_mOase.
    IPR020946. Flavin_mOase-like.
    IPR002254. Flavin_mOase_2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00743. FMO-like. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000332. FMO. 1 hit.
    PRINTSi PR00370. FMOXYGENASE.
    PR01122. FMOXYGENASE2.
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing, expression, and characterization of cDNA expressed flavin-containing monooxygenase 2 from mouse."
      Karoly E.D., Rose R.L.
      J. Biochem. Mol. Toxicol. 15:300-308(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
      Strain: C57BL/6.
      Tissue: Kidney.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiFMO2_MOUSE
    AccessioniPrimary (citable) accession number: Q8K2I3
    Secondary accession number(s): Q9QZF7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 102 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3