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Protein

Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1

Gene

Acap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration (By similarity).By similarity1 Publication

Miscellaneous

Cells overexpressing Acap1 show accumulation of an electron dense coat containing Acap1 and Cltc on internal membranes as well as accumulation of Tfrc in pericentriolar recycling endosomes. Adipocytes with reduced level of Acap1 or Cltc fail to transport SLC2A4/GLUT4 from recycling endosomes to the cell surface upon insulin stimulation.

Enzyme regulationi

GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri420 – 443C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
Alternative name(s):
Centaurin-beta-1
Short name:
Cnt-b1
Gene namesi
Name:Acap1
Synonyms:Centb1, Kiaa0050Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2388270 Acap1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi448R → Q: Loss of catalytic activity. No loss of accumulation of coat proteins on internal membranes upon overexpression of Acap1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003063841 – 740Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1Add BLAST740

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei485Nitrated tyrosineBy similarity1

Keywords - PTMi

Nitration

Proteomic databases

EPDiQ8K2H4
MaxQBiQ8K2H4
PaxDbiQ8K2H4
PRIDEiQ8K2H4

PTM databases

iPTMnetiQ8K2H4
PhosphoSitePlusiQ8K2H4

Expressioni

Gene expression databases

BgeeiENSMUSG00000001588
CleanExiMM_CENTB1
ExpressionAtlasiQ8K2H4 baseline and differential
GenevisibleiQ8K2H4 MM

Interactioni

Subunit structurei

Banana-shaped homodimer laterally assembling into tetramers, the tetramers further pack helically onto the membrane. Interacts with GTP-bound ARF6. Interacts with third cytoplasmic loop of SLC2A4/GLUT4. Interacts with CLTC. Interacts with GULP1. Forms a complex with GDP-bound ARF6 and GULP1. Interacts with ITGB1; required for ITGB1 recycling.By similarity1 Publication

Protein-protein interaction databases

IntActiQ8K2H4, 1 interactor
STRINGi10090.ENSMUSP00000001631

Structurei

3D structure databases

ProteinModelPortaliQ8K2H4
SMRiQ8K2H4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 226BARSequence analysisAdd BLAST226
Domaini265 – 360PHPROSITE-ProRule annotationAdd BLAST96
Domaini405 – 527Arf-GAPPROSITE-ProRule annotationAdd BLAST123
Repeati606 – 635ANK 1Sequence analysisAdd BLAST30
Repeati639 – 668ANK 2Sequence analysisAdd BLAST30
Repeati672 – 702ANK 3Sequence analysisAdd BLAST31

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 382Required for formation of endosomal tubules when overexpressed with PIP5K1CBy similarityAdd BLAST382
Regioni405 – 740Required for interaction with GULP1By similarityAdd BLAST336
Regioni525 – 566Prevents interaction with ITGB1 when S-554 is not phosphorylatedBy similarityAdd BLAST42

Domaini

PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes. Only one PH domain of one ACAP1 dimer inserts into the membrane, while the other PH domain acts primaryly to interact with adjacent ACAP1 dimers (By similarity).By similarity
The BAR domain mediates homodimerization, it can neither bind membrane nor impart curvature, but instead requires the neighboring PH domain to achieve these functions (By similarity).By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri420 – 443C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0521 Eukaryota
COG5347 LUCA
GeneTreeiENSGT00760000118874
HOGENOMiHOG000220815
HOVERGENiHBG050889
InParanoidiQ8K2H4
KOiK12489
OMAiGPPEPMM
OrthoDBiEOG091G029F
PhylomeDBiQ8K2H4
TreeFamiTF318315

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.20.1270.60, 1 hit
1.25.40.20, 1 hit
2.30.29.30, 1 hit
3.30.40.160, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR037278 ARFGAP/RecO
IPR001164 ArfGAP_dom
IPR038508 ArfGAP_dom_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000909 PLipase_C_PInositol-sp_X_dom
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF01412 ArfGap, 1 hit
PF00169 PH, 1 hit
PRINTSiPR00405 REVINTRACTNG
SMARTiView protein in SMART
SM00248 ANK, 3 hits
SM00105 ArfGap, 1 hit
SM00233 PH, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF48403 SSF48403, 1 hit
SSF57863 SSF57863, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 2 hits
PS50115 ARFGAP, 1 hit
PS50003 PH_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q8K2H4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVKLDFEEC LKDSPRFRAS IELVETEVSE LETRLEKLLK LGSCLLESGQ
60 70 80 90 100
HYLAAGRAFV VGICDLARLG PPEPMMAECL EKFTVSLNHK LDSHAELLDA
110 120 130 140 150
TQHTLQQQIQ TLVKEGLRGF REARRDFWRG AENLEAALTH NAEVPRRRVQ
160 170 180 190 200
EAEEAGTALR TARAGYRSRA LDYALQVNVI EDKRKFDIME FVLRLVEAQA
210 220 230 240 250
TYFQQGHEEL NRLAQYRKEL GTQLHNLVLN SARQKRDMEQ RHVLLKQKEL
260 270 280 290 300
GGEEPEPSLK EGPSGLVMEG HLFKRASNAF KTWSRRWFTI QNNQLVYQKK
310 320 330 340 350
YKDPVTVVVD DLRLCTVKLC PDSERRFCFE VVSTSKSCFL QADSERLLQL
360 370 380 390 400
WVSAVQSSIA SAFSQAHLEN SPRGPGQVSG YHAPGSAATL ACGGAARGRE
410 420 430 440 450
SGGVGQVAAQ VQSVDGNAQC CDCREPAPEW ASINLGVTLC IQCSGIHRSL
460 470 480 490 500
GVHFSKVRSL TLDSWEPELV KLMCELGNVI INQIYEARVE AMAVKKPGPS
510 520 530 540 550
CSRQEKEAWI HAKYVEKKFL TKLPEIRGRR GGRGPPRGHP PVPPKPPIRP
560 570 580 590 600
HSGIVRSKSE CPSDDMGSLH PGALLFQAAG HPPSLPTMAD ALAHGADVNW
610 620 630 640 650
VNVGQGNATP LIRATAANSL LACEFLLQNG ANVNQADSAG RGPLHHATIL
660 670 680 690 700
GHTGLACLFL KRGADLGARD TEGRDPLTIA METTNADIVT LLRLAKMREA
710 720 730 740
EAAQGQAGDE TYLDIFRDFS LMASDDPEKL SRRSHDLHTL
Length:740
Mass (Da):81,704
Last modified:October 1, 2002 - v1
Checksum:i798A87CBCC5F8513
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti522K → R in BAE32594 (PubMed:16141072).Curated1
Sequence conflicti584S → H in BAD90138 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK154449 mRNA Translation: BAE32594.1
AL596185, AL845465 Genomic DNA Translation: CAI35146.1
AL845465, AL596185 Genomic DNA Translation: CAM13886.1
BC031462 mRNA Translation: AAH31462.1
AK220213 mRNA Translation: BAD90138.1
CCDSiCCDS24919.1
RefSeqiNP_722483.2, NM_153788.3
UniGeneiMm.288671

Genome annotation databases

EnsembliENSMUST00000001631; ENSMUSP00000001631; ENSMUSG00000001588
GeneIDi216859
KEGGimmu:216859
UCSCiuc007jsf.1 mouse

Similar proteinsi

Entry informationi

Entry nameiACAP1_MOUSE
AccessioniPrimary (citable) accession number: Q8K2H4
Secondary accession number(s): Q3U441, Q571H6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health