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Protein

Bromodomain-containing protein 3

Gene

Brd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription of the CCND1 gene (By similarity). Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Bromodomain-containing protein 3
Alternative name(s):
Bromodomain-containing FSH-like protein FSRG2
Gene namesi
Name:Brd3
Synonyms:Fsrg2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1914632. Brd3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002111821 – 726Bromodomain-containing protein 3Add BLAST726

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei262PhosphoserineCombined sources1
Modified residuei280PhosphoserineBy similarity1
Modified residuei564PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8K2F0.
PaxDbiQ8K2F0.
PRIDEiQ8K2F0.

PTM databases

iPTMnetiQ8K2F0.
PhosphoSitePlusiQ8K2F0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026918.
CleanExiMM_BRD3.
ExpressionAtlasiQ8K2F0. baseline and differential.
GenevisibleiQ8K2F0. MM.

Interactioni

Subunit structurei

Interacts (via bromo domains) with acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (in vitro) (By similarity). Interacts (via bromo domain 1) with GATA1 acetylated at 'Lys-312' and 'Lys-315'. Interacts (via bromo domain 1) with GATA2 acetylated on lysine residues.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212148. 2 interactors.
IntActiQ8K2F0. 1 interactor.
MINTiMINT-4089355.
STRINGi10090.ENSMUSP00000109574.

Structurei

Secondary structure

1726
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi28 – 30Combined sources3
Helixi36 – 43Combined sources8
Helixi45 – 50Combined sources6
Helixi56 – 58Combined sources3
Turni65 – 68Combined sources4
Helixi72 – 75Combined sources4
Beta strandi76 – 78Combined sources3
Helixi83 – 90Combined sources8
Beta strandi94 – 96Combined sources3
Helixi97 – 99Combined sources3
Helixi100 – 114Combined sources15
Helixi120 – 136Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L5ENMR-A24-146[»]
ProteinModelPortaliQ8K2F0.
SMRiQ8K2F0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8K2F0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 122Bromo 1PROSITE-ProRule annotationAdd BLAST73
Domaini325 – 397Bromo 2PROSITE-ProRule annotationAdd BLAST73
Domaini563 – 645NETPROSITE-ProRule annotationAdd BLAST83

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili452 – 482Sequence analysisAdd BLAST31
Coiled coili646 – 689Sequence analysisAdd BLAST44

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi178 – 234Pro-richAdd BLAST57
Compositional biasi486 – 556Lys-richAdd BLAST71
Compositional biasi565 – 569Poly-Glu5
Compositional biasi691 – 725Ser-richAdd BLAST35

Sequence similaritiesi

Contains 2 bromo domains.PROSITE-ProRule annotation
Contains 1 NET domain.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain, Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1474. Eukaryota.
COG5076. LUCA.
GeneTreeiENSGT00760000119206.
HOGENOMiHOG000231200.
HOVERGENiHBG004896.
InParanoidiQ8K2F0.
KOiK11721.
OMAiAGRQPKK.
TreeFamiTF317345.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view]
PfamiPF17035. BET. 1 hit.
PF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K2F0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTTAAAPTG IPAVPGPVNP PPPEVSNPSK PGRKTNQLQY MQNVVVKTLW
60 70 80 90 100
KHQFAWPFYQ PVDAIKLNLP DYHKIIKNPM DMGTIKKRLE NNYYWSASEC
110 120 130 140 150
MQDFNTMFTN CYIYNKPTDD IVLMAQALEK IFLQKVAQMP QEEVELLPPA
160 170 180 190 200
PKGKGRKPAA GAQNAGSQQV AAVSSVSPAT PFQNIPPTVS QTPVIAATPV
210 220 230 240 250
PTITANVTSV PVPPPAAPPP PATPIVPVVP PTPPVVKKKG VKRKADTTTP
260 270 280 290 300
TTSAITASRS ESPPPLSEPK QAKVVARRES GGRPIKPPKK DLEDGEVPQH
310 320 330 340 350
AGKKGKLSEH LRHCDSILRE MLSKKHAAYA WPFYKPVDAE ALELHDYHDI
360 370 380 390 400
IKHPMDLSTV KRKMDSREYP DAQGFAADIR LMFSNCYKYN PPDHEVVAMA
410 420 430 440 450
RKLQDVFEMR FAKMPDEPME APALPAPTAP IVSKGAESSR SSEESSSDSG
460 470 480 490 500
SSDSEEERAT RLAELQEQLK AVHEQLAALS QAPVNKPKKK KEKKEKEKKK
510 520 530 540 550
KDKDKDKEKE KHKAKSEEEK KAKAAPAAKQ AQQKKAPTKK ANSTTTASRQ
560 570 580 590 600
LKKGGKQASA SYDSEEEEEG LPMSYDEKRQ LSLDINRLPG EKLGRVVHII
610 620 630 640 650
QSREPSLRDS NPDEIEIDFE TLKPTTLREL ERYVKSCLQK KQRKPLSTSG
660 670 680 690 700
KKQAAKSKEE LAQEKKKELE KRLQDVSGQL NSKKPTKKEK SGSAPSGGPS
710 720
RLSSSSSSES ASSSSSGSSS DSSDSE
Length:726
Mass (Da):79,762
Last modified:May 10, 2004 - v2
Checksum:iD5B2FC0ACA41D8ED
GO
Isoform 2 (identifier: Q8K2F0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     468-468: Q → QTGCGAFQDQLLNVSSVQ

Note: No experimental confirmation available.
Show »
Length:743
Mass (Da):81,511
Checksum:iEA325174381A3737
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti174S → F in BAC36359 (PubMed:16141072).Curated1
Sequence conflicti216A → T in AAH31536 (PubMed:15489334).Curated1
Sequence conflicti477A → D in AAF78072 (Ref. 1) Curated1
Sequence conflicti659 – 660EE → VV in AAF78072 (Ref. 1) Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010249468Q → QTGCGAFQDQLLNVSSVQ in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF269193 mRNA. Translation: AAF78072.1.
BC031536 mRNA. Translation: AAH31536.1.
AK037435 mRNA. Translation: BAC29806.1.
AK076472 mRNA. Translation: BAC36359.1.
CCDSiCCDS15828.1. [Q8K2F0-1]
CCDS50546.1. [Q8K2F0-2]
RefSeqiNP_001107045.1. NM_001113573.1. [Q8K2F0-1]
NP_001107046.1. NM_001113574.1. [Q8K2F0-2]
NP_075825.3. NM_023336.4. [Q8K2F0-1]
UniGeneiMm.28721.

Genome annotation databases

EnsembliENSMUST00000028282; ENSMUSP00000028282; ENSMUSG00000026918. [Q8K2F0-1]
ENSMUST00000077737; ENSMUSP00000076918; ENSMUSG00000026918. [Q8K2F0-1]
ENSMUST00000113941; ENSMUSP00000109574; ENSMUSG00000026918. [Q8K2F0-2]
ENSMUST00000164296; ENSMUSP00000128812; ENSMUSG00000026918. [Q8K2F0-1]
GeneIDi67382.
KEGGimmu:67382.
UCSCiuc008ixm.2. mouse. [Q8K2F0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF269193 mRNA. Translation: AAF78072.1.
BC031536 mRNA. Translation: AAH31536.1.
AK037435 mRNA. Translation: BAC29806.1.
AK076472 mRNA. Translation: BAC36359.1.
CCDSiCCDS15828.1. [Q8K2F0-1]
CCDS50546.1. [Q8K2F0-2]
RefSeqiNP_001107045.1. NM_001113573.1. [Q8K2F0-1]
NP_001107046.1. NM_001113574.1. [Q8K2F0-2]
NP_075825.3. NM_023336.4. [Q8K2F0-1]
UniGeneiMm.28721.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L5ENMR-A24-146[»]
ProteinModelPortaliQ8K2F0.
SMRiQ8K2F0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212148. 2 interactors.
IntActiQ8K2F0. 1 interactor.
MINTiMINT-4089355.
STRINGi10090.ENSMUSP00000109574.

PTM databases

iPTMnetiQ8K2F0.
PhosphoSitePlusiQ8K2F0.

Proteomic databases

EPDiQ8K2F0.
PaxDbiQ8K2F0.
PRIDEiQ8K2F0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028282; ENSMUSP00000028282; ENSMUSG00000026918. [Q8K2F0-1]
ENSMUST00000077737; ENSMUSP00000076918; ENSMUSG00000026918. [Q8K2F0-1]
ENSMUST00000113941; ENSMUSP00000109574; ENSMUSG00000026918. [Q8K2F0-2]
ENSMUST00000164296; ENSMUSP00000128812; ENSMUSG00000026918. [Q8K2F0-1]
GeneIDi67382.
KEGGimmu:67382.
UCSCiuc008ixm.2. mouse. [Q8K2F0-1]

Organism-specific databases

CTDi8019.
MGIiMGI:1914632. Brd3.

Phylogenomic databases

eggNOGiKOG1474. Eukaryota.
COG5076. LUCA.
GeneTreeiENSGT00760000119206.
HOGENOMiHOG000231200.
HOVERGENiHBG004896.
InParanoidiQ8K2F0.
KOiK11721.
OMAiAGRQPKK.
TreeFamiTF317345.

Miscellaneous databases

ChiTaRSiBrd3. mouse.
EvolutionaryTraceiQ8K2F0.
PROiQ8K2F0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026918.
CleanExiMM_BRD3.
ExpressionAtlasiQ8K2F0. baseline and differential.
GenevisibleiQ8K2F0. MM.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view]
PfamiPF17035. BET. 1 hit.
PF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRD3_MOUSE
AccessioniPrimary (citable) accession number: Q8K2F0
Secondary accession number(s): Q8C665, Q8CAX7, Q9JI25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: November 2, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.