Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3

Gene

Hacd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. Involved in Rac1-signaling pathways leading to the modulation of gene expression. Promotes insulin receptor/INSR autophosphorylation and is involved in INSR internalization (By similarity).By similarity

Catalytic activityi

A very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain trans-2,3-dehydroacyl-CoA + H2O.By similarity

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei286By similarity1
Active sitei293By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3Curated (EC:4.2.1.134By similarity)
Alternative name(s):
3-hydroxyacyl-CoA dehydratase 3Curated
Short name:
HACD3Curated
Butyrate-induced protein 11 Publication
Short name:
B-ind11 Publication
Protein-tyrosine phosphatase-like A domain-containing protein 1Imported
Gene namesi
Name:Hacd3Imported
Synonyms:Ptplad1Curated
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1889341. Hacd3.

Subcellular locationi

  • Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 149CytoplasmicSequence analysisAdd BLAST149
Transmembranei150 – 170HelicalSequence analysisAdd BLAST21
Topological domaini171 – 189LumenalSequence analysisAdd BLAST19
Transmembranei190 – 210HelicalSequence analysisAdd BLAST21
Topological domaini211 – 212CytoplasmicSequence analysis2
Transmembranei213 – 233HelicalSequence analysisAdd BLAST21
Topological domaini234 – 242LumenalSequence analysis9
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 280CytoplasmicSequence analysisAdd BLAST17
Transmembranei281 – 301HelicalSequence analysisAdd BLAST21
Topological domaini302 – 322LumenalSequence analysisAdd BLAST21
Transmembranei323 – 343HelicalSequence analysisAdd BLAST21
Topological domaini344 – 362CytoplasmicSequence analysisAdd BLAST19

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003137251 – 362Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3Add BLAST362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei7PhosphothreonineBy similarity1
Modified residuei114PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8K2C9.
MaxQBiQ8K2C9.
PaxDbiQ8K2C9.
PeptideAtlasiQ8K2C9.
PRIDEiQ8K2C9.

PTM databases

iPTMnetiQ8K2C9.
PhosphoSitePlusiQ8K2C9.
SwissPalmiQ8K2C9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000033629.
GenevisibleiQ8K2C9. MM.

Interactioni

Subunit structurei

May interact with enzymes of the ELO family (including ELOVL1); with those enzymes that mediate condensation, the first of the four steps of the reaction cycle responsible for fatty acids elongation, may be part of a larger fatty acids elongase complex. Interacts with RAC1. Associates with internalized insulin receptor/INSR complexes on Golgi/endosomal membranes; HACD3/PTPLAD1 together with ATIC and PRKAA2/AMPK2 is proposed to be part of a signaling network regulating INSR autophosphorylation and endocytosis (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PSMD2Q132002EBI-8329978,EBI-357648From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8K2C9. 91 interactors.
MINTiMINT-1858563.
STRINGi10090.ENSMUSP00000044955.

Structurei

3D structure databases

ProteinModelPortaliQ8K2C9.
SMRiQ8K2C9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 94CSPROSITE-ProRule annotationAdd BLAST90

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili111 – 138Sequence analysisAdd BLAST28

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi350 – 353Poly-Arg4
Compositional biasi357 – 360Poly-Lys4

Sequence similaritiesi

Contains 1 CS domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3187. Eukaryota.
COG5198. LUCA.
GeneTreeiENSGT00530000062962.
HOGENOMiHOG000008043.
HOVERGENiHBG108301.
InParanoidiQ8K2C9.
KOiK10703.
OMAiSYLVMSH.
OrthoDBiEOG091G09PF.
PhylomeDBiQ8K2C9.
TreeFamiTF313326.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
InterProiIPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
IPR007482. Tyr_Pase-like_PTPLA.
[Graphical view]
PANTHERiPTHR11035. PTHR11035. 1 hit.
PfamiPF04387. PTPLA. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 1 hit.
PROSITEiPS51203. CS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K2C9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METQVLTPHV YWAQRHRELY LRVELSDVQN PAISITDNVL HFKAQGHGAK
60 70 80 90 100
GDNVYEFHLE FLDLVKPEPA YRLTQRQVNI TVQKKGSHWW ERLTKQEKRP
110 120 130 140 150
LFLAPDFDRW LDESDAEMEL RAKEEERLNK LRLEREGSPE TLTNLKKGYL
160 170 180 190 200
FMYNLVQLLG FSWIFVNLTV RFFILGKESF YDTFHNVADM MYFCQMLALV
210 220 230 240 250
ETLNAAIGVT STPVLPALIQ FLGRNFILFL VFGTMEEMQN KAVVFFVFYS
260 270 280 290 300
WSAIEIFRYP FYMLSCIDMD WKVLTWLRYT MWIPLYPLGC LSEAVAVIQS
310 320 330 340 350
IPVFNESGRF SFTLPYPVKM KVRFSFFLQV YLVMLFLGLY INFRHLYKQR
360
RRRYGQKKKK LH
Length:362
Mass (Da):43,131
Last modified:January 15, 2008 - v2
Checksum:i85716941EF7008F6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32A → P in AAH57023 (PubMed:15489334).Curated1
Sequence conflicti63D → E in AAH31755 (PubMed:15489334).Curated1
Sequence conflicti146K → R in CAB10097 (PubMed:10747961).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK051735 mRNA. Translation: BAC34744.1.
AK052865 mRNA. Translation: BAC35179.1.
AK050556 mRNA. Translation: BAE20677.1.
AK159494 mRNA. Translation: BAE35128.1.
BC031755 mRNA. Translation: AAH31755.1.
BC057023 mRNA. Translation: AAH57023.1.
Z97207 mRNA. Translation: CAB10097.2.
CCDSiCCDS23284.1.
RefSeqiNP_067320.2. NM_021345.2.
UniGeneiMm.308180.

Genome annotation databases

EnsembliENSMUST00000036615; ENSMUSP00000044955; ENSMUSG00000033629.
GeneIDi57874.
KEGGimmu:57874.
UCSCiuc009qcp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK051735 mRNA. Translation: BAC34744.1.
AK052865 mRNA. Translation: BAC35179.1.
AK050556 mRNA. Translation: BAE20677.1.
AK159494 mRNA. Translation: BAE35128.1.
BC031755 mRNA. Translation: AAH31755.1.
BC057023 mRNA. Translation: AAH57023.1.
Z97207 mRNA. Translation: CAB10097.2.
CCDSiCCDS23284.1.
RefSeqiNP_067320.2. NM_021345.2.
UniGeneiMm.308180.

3D structure databases

ProteinModelPortaliQ8K2C9.
SMRiQ8K2C9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8K2C9. 91 interactors.
MINTiMINT-1858563.
STRINGi10090.ENSMUSP00000044955.

PTM databases

iPTMnetiQ8K2C9.
PhosphoSitePlusiQ8K2C9.
SwissPalmiQ8K2C9.

Proteomic databases

EPDiQ8K2C9.
MaxQBiQ8K2C9.
PaxDbiQ8K2C9.
PeptideAtlasiQ8K2C9.
PRIDEiQ8K2C9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036615; ENSMUSP00000044955; ENSMUSG00000033629.
GeneIDi57874.
KEGGimmu:57874.
UCSCiuc009qcp.2. mouse.

Organism-specific databases

CTDi51495.
MGIiMGI:1889341. Hacd3.

Phylogenomic databases

eggNOGiKOG3187. Eukaryota.
COG5198. LUCA.
GeneTreeiENSGT00530000062962.
HOGENOMiHOG000008043.
HOVERGENiHBG108301.
InParanoidiQ8K2C9.
KOiK10703.
OMAiSYLVMSH.
OrthoDBiEOG091G09PF.
PhylomeDBiQ8K2C9.
TreeFamiTF313326.

Enzyme and pathway databases

UniPathwayiUPA00094.

Miscellaneous databases

ChiTaRSiPtplad1. mouse.
PROiQ8K2C9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033629.
GenevisibleiQ8K2C9. MM.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
InterProiIPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
IPR007482. Tyr_Pase-like_PTPLA.
[Graphical view]
PANTHERiPTHR11035. PTHR11035. 1 hit.
PfamiPF04387. PTPLA. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 1 hit.
PROSITEiPS51203. CS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHACD3_MOUSE
AccessioniPrimary (citable) accession number: Q8K2C9
Secondary accession number(s): O09003, Q6PGH3, Q8BGM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: November 2, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Shares some similarity with tyrosine phosphatase proteins but it has probably no phosphatase activity.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.