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Protein

Glycerol-3-phosphate acyltransferase 4

Gene

Gpat4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Active against both saturated and unsaturated long-chain fatty acyl-CoAs (By similarity).By similarity1 Publication

Catalytic activityi

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase 1, mitochondrial (Gpam), Glycerol-3-phosphate acyltransferase 4 (Gpat4), Glycerol-3-phosphate acyltransferase 3 (Gpat3), Glycerol-3-phosphate acyltransferase 2, mitochondrial (Gpat2)
  2. 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (Agpat3), 1-acyl-sn-glycerol-3-phosphate acyltransferase beta (Agpat2), 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (Agpat1), Lysocardiolipin acyltransferase 1 (Lclat1), 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon (Agpat5), 1-acyl-sn-glycerol-3-phosphate acyltransferase delta (Agpat4)
  3. Phosphatidate cytidylyltransferase, mitochondrial (Tamm41), Phosphatidate cytidylyltransferase, mitochondrial (Tamm41), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase 2 (Cds2), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase 1 (Cds1)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • glycerol-3-phosphate O-acyltransferase activity Source: UniProtKB
  • sn-1-glycerol-3-phosphate C16:0-DCA-CoA acyl transferase activity Source: UniProtKB-EC

GO - Biological processi

  • acyl-CoA metabolic process Source: MGI
  • CDP-diacylglycerol biosynthetic process Source: UniProtKB-UniPathway
  • diacylglycerol metabolic process Source: MGI
  • fatty acid metabolic process Source: MGI
  • glandular epithelial cell maturation Source: MGI
  • lactation Source: UniProtKB
  • lipid biosynthetic process Source: UniProtKB
  • mammary gland development Source: MGI
  • phosphatidylcholine biosynthetic process Source: MGI
  • regulation of multicellular organism growth Source: MGI
  • triglyceride biosynthetic process Source: UniProtKB
  • triglyceride metabolic process Source: MGI

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BRENDAi2.3.1.15 3474
ReactomeiR-MMU-1483166 Synthesis of PA
UniPathwayiUPA00557; UER00612

Chemistry databases

SwissLipidsiSLP:000000104

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate acyltransferase 4Imported (EC:2.3.1.15)
Short name:
GPAT4
Alternative name(s):
1-acylglycerol-3-phosphate O-acyltransferase 6
Short name:
1-AGP acyltransferase 6
Short name:
1-AGPAT 6
Acyl-CoA:glycerol-3-phosphate acyltransferase 4
Lysophosphatidic acid acyltransferase zeta
Short name:
LPAAT-zeta
Gene namesi
Name:Gpat4Imported
Synonyms:Agpat6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2142716 Gpat4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei156 – 176HelicalSequence analysisAdd BLAST21
Transmembranei180 – 200HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37Sequence analysisAdd BLAST37
ChainiPRO_000002470438 – 456Glycerol-3-phosphate acyltransferase 4Add BLAST419

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi247N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi327N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi328N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi362N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ8K2C8
MaxQBiQ8K2C8
PaxDbiQ8K2C8
PRIDEiQ8K2C8

PTM databases

iPTMnetiQ8K2C8
PhosphoSitePlusiQ8K2C8

Expressioni

Gene expression databases

BgeeiENSMUSG00000031545
ExpressionAtlasiQ8K2C8 baseline and differential
GenevisibleiQ8K2C8 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000127325

Structurei

3D structure databases

ProteinModelPortaliQ8K2C8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi248 – 253HXXXXD motif6

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2898 Eukaryota
COG0204 LUCA
GeneTreeiENSGT00390000000536
HOGENOMiHOG000265725
InParanoidiQ8K2C8
KOiK13506
OMAiKMLVGTQ
OrthoDBiEOG091G04E8
PhylomeDBiQ8K2C8
TreeFamiTF315039

Family and domain databases

InterProiView protein in InterPro
IPR002123 Plipid/glycerol_acylTrfase
PfamiView protein in Pfam
PF01553 Acyltransferase, 1 hit
SMARTiView protein in SMART
SM00563 PlsC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K2C8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLLLPFDSL IVNLLGISLT VLFTLLLVFI IVPAIFGVSF GIRKLYMKTL
60 70 80 90 100
LKIFAWATLR MERGAKERNH QLYKPYTNGI IAKDPTSLEE EIKEIRRSGS
110 120 130 140 150
SKALDKTPEF ELSDIFYFCR KGMETIMDDE VTKRFSAEEL ESWNLLSRTN
160 170 180 190 200
YNFQYISLRL TILWGLGVLI RYCFLLPLRI ALAFTGIGLL VVGTTMVGYL
210 220 230 240 250
PNGRFKEFLS KHVHLMCYRI CVRALTAIIT YHNRKNRPRN GGICVANHTS
260 270 280 290 300
PIDVIILASD GYYAMVGQVH GGLMGVIQRA MVKACPHVWF ERSEVKDRHL
310 320 330 340 350
VAKRLTEHVQ DKSKLPILIF PEGTCINNTS VMMFKKGSFE IGATVYPVAI
360 370 380 390 400
KYDPQFGDAF WNSSKYGMVT YLLRMMTSWA IVCSVWYLPP MTREKDEDAV
410 420 430 440 450
QFANRVKSAI ARQGGLVDLL WDGGLKREKV KDTFKEEQQK LYSKMIVGNH

EDRSRS
Length:456
Mass (Da):52,181
Last modified:October 1, 2002 - v1
Checksum:i2C3E1C37044C14FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF406611 mRNA Translation: AAP97283.1
AK045235 mRNA Translation: BAC32273.1
AK077561 mRNA Translation: BAC36864.1
AK083589 mRNA Translation: BAC38962.1
AK161270 mRNA Translation: BAE36282.1
AK169257 mRNA Translation: BAE41020.1
BC031767 mRNA Translation: AAH31767.1
CCDSiCCDS22189.1
RefSeqiNP_061213.2, NM_018743.4
UniGeneiMm.200898
Mm.241152

Genome annotation databases

EnsembliENSMUST00000167004; ENSMUSP00000127325; ENSMUSG00000031545
GeneIDi102247
KEGGimmu:102247
UCSCiuc009leo.2 mouse

Similar proteinsi

Entry informationi

Entry nameiGPAT4_MOUSE
AccessioniPrimary (citable) accession number: Q8K2C8
Secondary accession number(s): Q3TF78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome