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Protein

Protein OS-9

Gene

Os9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei130 – 1301CarbohydrateBy similarity
Binding sitei182 – 1821CarbohydrateBy similarity
Binding sitei188 – 1881CarbohydrateBy similarity
Binding sitei212 – 2121CarbohydrateBy similarity
Binding sitei218 – 2181CarbohydrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Lectin

Enzyme and pathway databases

ReactomeiR-MMU-5358346. Hedgehog ligand biogenesis.
R-MMU-901032. ER Quality Control Compartment (ERQC).

Names & Taxonomyi

Protein namesi
Recommended name:
Protein OS-9
Gene namesi
Name:Os9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1924301. Os9.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence analysisAdd
BLAST
Chaini27 – 672646Protein OS-9PRO_0000386449Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi110 ↔ 123By similarity
Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence analysis
Disulfide bondi181 ↔ 216By similarity
Disulfide bondi196 ↔ 228By similarity

Post-translational modificationi

Intramolecular disulfide bonds.By similarity
N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ8K2C7.
MaxQBiQ8K2C7.
PaxDbiQ8K2C7.
PRIDEiQ8K2C7.

PTM databases

iPTMnetiQ8K2C7.
PhosphoSiteiQ8K2C7.

Expressioni

Gene expression databases

BgeeiQ8K2C7.
GenevisibleiQ8K2C7. MM.

Interactioni

Subunit structurei

Probably part of the HRD1 ubiquitin ligase complex composed at least of SYVN1/HRD1 and SEL1L with which it interacts directly. Through this complex it may interact with ERLEC1 and HSPA5. Interacts with DERL2. Interacts with HSP90B1, CREB3 and SYVN1 (By similarity). Interacts (via C-terminus) with CPNE6 (via second C2 domain); this interaction occurs in a calcium-dependent manner in vitro (PubMed:10403379).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229745. 2 interactions.
IntActiQ8K2C7. 3 interactions.
MINTiMINT-4117264.
STRINGi10090.ENSMUSP00000128914.

Structurei

3D structure databases

ProteinModelPortaliQ8K2C7.
SMRiQ8K2C7. Positions 109-228.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini108 – 17871PRKCSHAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi412 – 43423Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the OS-9 family.Curated
Contains 1 PRKCSH domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3394. Eukaryota.
ENOG410XR8A. LUCA.
GeneTreeiENSGT00530000063603.
HOGENOMiHOG000293348.
InParanoidiQ8K2C7.
KOiK10088.
OMAiFQNNVQV.
OrthoDBiEOG7R8315.
PhylomeDBiQ8K2C7.
TreeFamiTF314309.

Family and domain databases

Gene3Di2.70.130.10. 1 hit.
InterProiIPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR012913. PRKCSH.
[Graphical view]
PfamiPF07915. PRKCSH. 1 hit.
[Graphical view]
SUPFAMiSSF50911. SSF50911. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K2C7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAEVLLSSL LGLLFLGLLL PARLTGGVGS LNLEELSEMR YGIQILPLPV
60 70 80 90 100
MGGQSQASDV VVVSSKYKQR YECRLPAGAI HFQREREEET PAYQGPGIPE
110 120 130 140 150
LLSPMRDAPC LLKTKDWWTY EFCYGRHIQQ YHMEDSEIKG DVLYLGHYQS
160 170 180 190 200
SFNWDDETAK ASKQHRLKRY HSQTYGNGSK CDLNGKPREA EVRFLCDEGA
210 220 230 240 250
GISGDYIDRV DEPVSCSYVL TIRTSRLCPH PLLRPPASAA PQAILCHPAL
260 270 280 290 300
QPDEYMAYLQ RQAESKQHEE KTTEEVQDTD RQVWSGSKAA GAPPKKEDVS
310 320 330 340 350
PAKEEKESEL WKLQGPEEQA AAREEAQAGE QDLNHEAAAD PAPSPPNDFQ
360 370 380 390 400
NNVQVKLIRS PADLIRLIEE LKAAEKGKPS VRREQPGDDT TEAPQREAEG
410 420 430 440 450
TKAKGKDGEP PGLMEEEDGD DEEEEEEEEE DEEEQQLLGE FEKELEGMLL
460 470 480 490 500
PSNRERLRSE VKAGMERELE NIIQETEKEL DPEGLRKESE REQAILALTS
510 520 530 540 550
TLDKLIKRLQ ENQSPELVQK YKKRRVVPQK PPPSPHPTEE EPEHRVRVRV
560 570 580 590 600
TKLRPGGPNR DLTVLEMNRE NPQLKQIEGL VTEVLEREGL TAEGKIEIKI
610 620 630 640 650
VRPGAEGKEE DTRWLTDEDT RNLKEIFFNI LVQGAEEANK ERQRQSELES
660 670
NYRRVWGSPG GEDTGDLDEF DF
Length:672
Mass (Da):76,108
Last modified:October 13, 2009 - v2
Checksum:i7320981E721A9686
GO
Isoform 2 (identifier: Q8K2C7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     539-593: Missing.

Show »
Length:617
Mass (Da):69,764
Checksum:iEC224EA8A3C07E8D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti407 – 4071D → G in AAH31768 (PubMed:15489334).Curated
Sequence conflicti453 – 4531N → D in AAH31768 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei539 – 59355Missing in isoform 2. 1 PublicationVSP_038220Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC131760 Genomic DNA. No translation available.
BC031768 mRNA. Translation: AAH31768.1.
CCDSiCCDS24229.1. [Q8K2C7-2]
CCDS48713.1. [Q8K2C7-1]
RefSeqiNP_001164497.1. NM_001171026.1. [Q8K2C7-1]
NP_808282.2. NM_177614.3. [Q8K2C7-2]
UniGeneiMm.295246.
Mm.403598.

Genome annotation databases

EnsembliENSMUST00000080975; ENSMUSP00000079770; ENSMUSG00000040462. [Q8K2C7-2]
ENSMUST00000164259; ENSMUSP00000128914; ENSMUSG00000040462. [Q8K2C7-1]
GeneIDi216440.
KEGGimmu:216440.
UCSCiuc007hhz.2. mouse. [Q8K2C7-2]
uc007hia.2. mouse. [Q8K2C7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC131760 Genomic DNA. No translation available.
BC031768 mRNA. Translation: AAH31768.1.
CCDSiCCDS24229.1. [Q8K2C7-2]
CCDS48713.1. [Q8K2C7-1]
RefSeqiNP_001164497.1. NM_001171026.1. [Q8K2C7-1]
NP_808282.2. NM_177614.3. [Q8K2C7-2]
UniGeneiMm.295246.
Mm.403598.

3D structure databases

ProteinModelPortaliQ8K2C7.
SMRiQ8K2C7. Positions 109-228.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229745. 2 interactions.
IntActiQ8K2C7. 3 interactions.
MINTiMINT-4117264.
STRINGi10090.ENSMUSP00000128914.

PTM databases

iPTMnetiQ8K2C7.
PhosphoSiteiQ8K2C7.

Proteomic databases

EPDiQ8K2C7.
MaxQBiQ8K2C7.
PaxDbiQ8K2C7.
PRIDEiQ8K2C7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000080975; ENSMUSP00000079770; ENSMUSG00000040462. [Q8K2C7-2]
ENSMUST00000164259; ENSMUSP00000128914; ENSMUSG00000040462. [Q8K2C7-1]
GeneIDi216440.
KEGGimmu:216440.
UCSCiuc007hhz.2. mouse. [Q8K2C7-2]
uc007hia.2. mouse. [Q8K2C7-1]

Organism-specific databases

CTDi10956.
MGIiMGI:1924301. Os9.

Phylogenomic databases

eggNOGiKOG3394. Eukaryota.
ENOG410XR8A. LUCA.
GeneTreeiENSGT00530000063603.
HOGENOMiHOG000293348.
InParanoidiQ8K2C7.
KOiK10088.
OMAiFQNNVQV.
OrthoDBiEOG7R8315.
PhylomeDBiQ8K2C7.
TreeFamiTF314309.

Enzyme and pathway databases

ReactomeiR-MMU-5358346. Hedgehog ligand biogenesis.
R-MMU-901032. ER Quality Control Compartment (ERQC).

Miscellaneous databases

PROiQ8K2C7.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K2C7.
GenevisibleiQ8K2C7. MM.

Family and domain databases

Gene3Di2.70.130.10. 1 hit.
InterProiIPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR012913. PRKCSH.
[Graphical view]
PfamiPF07915. PRKCSH. 1 hit.
[Graphical view]
SUPFAMiSSF50911. SSF50911. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "Ca2(+)-dependent interaction of N-copine, a member of the two C2 domain protein family, with OS-9, the product of a gene frequently amplified in osteosarcoma."
    Nakayama T., Yaoi T., Kuwajima G., Yoshie O., Sakata T.
    FEBS Lett. 453:77-80(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CPNE6.
  4. "Mammalian OS-9 is upregulated in response to endoplasmic reticulum stress and facilitates ubiquitination of misfolded glycoproteins."
    Alcock F., Swanton E.
    J. Mol. Biol. 385:1032-1042(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiOS9_MOUSE
AccessioniPrimary (citable) accession number: Q8K2C7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: October 13, 2009
Last modified: June 8, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.