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Protein

Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase

Gene

Alg3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc2-PP-Dol.

Catalytic activityi

Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathway:iprotein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_320215. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT58. Glycosyltransferase Family 58.

Names & Taxonomyi

Protein namesi
Recommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase (EC:2.4.1.258)
Alternative name(s):
Asparagine-linked glycosylation protein 3 homolog
Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase
Not56-like protein
Gene namesi
Name:Alg3
Synonyms:Not, Not56l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1098592. Alg3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei41 – 6121HelicalSequence AnalysisAdd
BLAST
Transmembranei95 – 11521HelicalSequence AnalysisAdd
BLAST
Transmembranei123 – 14321HelicalSequence AnalysisAdd
BLAST
Transmembranei149 – 16921HelicalSequence AnalysisAdd
BLAST
Transmembranei172 – 19221HelicalSequence AnalysisAdd
BLAST
Transmembranei203 – 22321HelicalSequence AnalysisAdd
BLAST
Transmembranei231 – 25121HelicalSequence AnalysisAdd
BLAST
Transmembranei289 – 30921HelicalSequence AnalysisAdd
BLAST
Transmembranei332 – 35221HelicalSequence AnalysisAdd
BLAST
Transmembranei356 – 37621HelicalSequence AnalysisAdd
BLAST
Transmembranei407 – 42721HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 438438Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferasePRO_0000080567Add
BLAST

Proteomic databases

MaxQBiQ8K2A8.
PaxDbiQ8K2A8.
PRIDEiQ8K2A8.

PTM databases

PhosphoSiteiQ8K2A8.

Expressioni

Gene expression databases

BgeeiQ8K2A8.
ExpressionAtlasiQ8K2A8. baseline and differential.
GenevisibleiQ8K2A8. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045272.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 58 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG241920.
GeneTreeiENSGT00390000013904.
HOGENOMiHOG000237555.
HOVERGENiHBG002070.
InParanoidiQ8K2A8.
KOiK03845.
OMAiTKKVPPY.
OrthoDBiEOG7CVPXS.
TreeFamiTF105870.

Family and domain databases

InterProiIPR007873. Glycosyltransferase_ALG3.
[Graphical view]
PANTHERiPTHR12646. PTHR12646. 1 hit.
PfamiPF05208. ALG3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K2A8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAGLRKRGQ PASVGQPAGI WKQWLQRAWQ ERYLLLREPR YTLLVASCLC
60 70 80 90 100
IAEVGITFWV IHRVAYTEID WKAYMAQVEG FINGTYDYTQ LQGDTGPLVY
110 120 130 140 150
PAGFLYIFTG LFYATDRGTD IPMAQNIFAV LYLVTLVLVF LIYHQTSKVP
160 170 180 190 200
PFVFFFMCCA SYRVHSIFVL RLFNDPVAMA LLFLSINLFL AQCWSWGCCC
210 220 230 240 250
FSLAVSVKMN VLLFAPGLLF LLLTQFGFRG ALPKLAICAA LQVVLGLPFL
260 270 280 290 300
LENPIGYLSR SFDLGRQFLF QWTVNWRFLP ETIFLHRAFH LALLAAHLSL
310 320 330 340 350
LLLFALCRWH RTGESILALL KDPSKRKVPP QALTPNQIVS ILFTSNFIGI
360 370 380 390 400
CFSRSLHYQF YVWYFHTLPY LLWAMPARWL THLLRLLVLG LIELSWNTYP
410 420 430
STSFSSAALH LCHAVVLLQL WLSPESFPKS IQPSRKTH
Length:438
Mass (Da):50,186
Last modified:July 27, 2011 - v2
Checksum:i2332C12F413C897F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti354 – 3541R → H in AAH31893 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC087898 Genomic DNA. No translation available.
BC031893 mRNA. Translation: AAH31893.1.
CCDSiCCDS37289.1.
RefSeqiNP_666051.2. NM_145939.2.
UniGeneiMm.24950.

Genome annotation databases

EnsembliENSMUST00000045918; ENSMUSP00000045272; ENSMUSG00000033809.
GeneIDi208624.
KEGGimmu:208624.
UCSCiuc007yqe.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC087898 Genomic DNA. No translation available.
BC031893 mRNA. Translation: AAH31893.1.
CCDSiCCDS37289.1.
RefSeqiNP_666051.2. NM_145939.2.
UniGeneiMm.24950.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045272.

Protein family/group databases

CAZyiGT58. Glycosyltransferase Family 58.

PTM databases

PhosphoSiteiQ8K2A8.

Proteomic databases

MaxQBiQ8K2A8.
PaxDbiQ8K2A8.
PRIDEiQ8K2A8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045918; ENSMUSP00000045272; ENSMUSG00000033809.
GeneIDi208624.
KEGGimmu:208624.
UCSCiuc007yqe.2. mouse.

Organism-specific databases

CTDi10195.
MGIiMGI:1098592. Alg3.

Phylogenomic databases

eggNOGiNOG241920.
GeneTreeiENSGT00390000013904.
HOGENOMiHOG000237555.
HOVERGENiHBG002070.
InParanoidiQ8K2A8.
KOiK03845.
OMAiTKKVPPY.
OrthoDBiEOG7CVPXS.
TreeFamiTF105870.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_320215. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

NextBioi372337.
PROiQ8K2A8.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K2A8.
ExpressionAtlasiQ8K2A8. baseline and differential.
GenevisibleiQ8K2A8. MM.

Family and domain databases

InterProiIPR007873. Glycosyltransferase_ALG3.
[Graphical view]
PANTHERiPTHR12646. PTHR12646. 1 hit.
PfamiPF05208. ALG3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiALG3_MOUSE
AccessioniPrimary (citable) accession number: Q8K2A8
Secondary accession number(s): E9QKP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: July 27, 2011
Last modified: July 22, 2015
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.