Reviewed, UniProtKB/Swiss-Prot Q8K2A8 (ALG3_MOUSE)

Names and origin

Protein names

Recommended name:
    Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
    EC=2.4.1.130
Alternative name(s):
    Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
    Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase
    Asparagine-linked glycosylation protein 3 homolog
    Not56-like protein

Gene names

Name:	Alg3
Synonyms:	Not, Not56l

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc2-PP-Dol.
Catalytic activity	Transfers an alpha-D-mannosyl residue from dolichyl-phosphate D-mannose into membrane lipid-linked oligosaccharide.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the glycosyltransferase 58 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase		PRO_0000080567
Regions
	Transmembrane	41 – 61	21	Potential
	Transmembrane	95 – 115	21	Potential
	Transmembrane	123 – 143	21	Potential
	Transmembrane	149 – 169	21	Potential
	Transmembrane	172 – 192	21	Potential
	Transmembrane	203 – 223	21	Potential
	Transmembrane	231 – 251	21	Potential
	Transmembrane	289 – 309	21	Potential
	Transmembrane	332 – 352	21	Potential
	Transmembrane	356 – 376	21	Potential
	Transmembrane	407 – 427	21	Potential
Amino acid modifications
	Modified residue	13	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K2A8-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 99297B2F51F6330F	FASTA	438	50,166
	10 20 30 40 50 60 MAAGLRKRGQ PASVGQPAGI WKQWLQRAWQ ERYLLLREPR YTLLVASCLC IAEVGITFWV 70 80 90 100 110 120 IHRVAYTEID WKAYMAQVEG FINGTYDYTQ LQGDTGPLVY PAGFLYIFTG LFYATDRGTD 130 140 150 160 170 180 IPMAQNIFAV LYLVTLVLVF LIYHQTSKVP PFVFFFMCCA SYRVHSIFVL RLFNDPVAMA 190 200 210 220 230 240 LLFLSINLFL AQCWSWGCCC FSLAVSVKMN VLLFAPGLLF LLLTQFGFRG ALPKLAICAA 250 260 270 280 290 300 LQVVLGLPFL LENPIGYLSR SFDLGRQFLF QWTVNWRFLP ETIFLHRAFH LALLAAHLSL 310 320 330 340 350 360 LLLFALCRWH RTGESILALL KDPSKRKVPP QALTPNQIVS ILFTSNFIGI CFSHSLHYQF 370 380 390 400 410 420 YVWYFHTLPY LLWAMPARWL THLLRLLVLG LIELSWNTYP STSFSSAALH LCHAVVLLQL 430 WLSPESFPKS IQPSRKTH

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N-3. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC031893 mRNA. Translation: AAH31893.1.
IPI	IPI00322103.
UniGene	Mm.24950
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8K2A8.
Protein family/group databases
CAZy	GT58. Glycosyltransferase Family 58.
PTM databases
PhosphoSite	Q8K2A8.
Proteomic databases
PRIDE	Q8K2A8.
Genome annotation databases
Ensembl	ENSMUST00000045918; ENSMUSP00000045272; ENSMUSG00000033809; Mus musculus. [Genome view]
UCSC	uc007yqe.1. mouse.
Organism-specific databases
MGI	MGI:1098592. Alg3.
Phylogenomic databases
eggNOG	maNOG18429.
HOGENOM	HBG525447.
HOVERGEN	Q8K2A8.
InParanoid	Q8K2A8.
PhylomeDB	Q8K2A8.
Enzyme and pathway databases
BRENDA	2.4.1.130. 244.
Gene expression databases
ArrayExpress	Q8K2A8.
Bgee	Q8K2A8.
Genevestigator	Q8K2A8.
GermOnline	ENSMUSG00000033809. Mus musculus.
Family and domain databases
InterPro	IPR007873. Glycosyltransferase_ALG3. [Graphical view]
PANTHER	PTHR12646. ALG3. 1 hit.
Pfam	PF05208. ALG3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	372337.
SOURCE	Search...

Entry information

Entry name

ALG3_MOUSE

Accession

Primary (citable) accession number: Q8K2A8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)