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Q8K2A8

- ALG3_MOUSE

UniProt

Q8K2A8 - ALG3_MOUSE

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Protein

Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase

Gene

Alg3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc2-PP-Dol.

Catalytic activityi

Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi

GO - Molecular functioni

  1. dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_262413. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT58. Glycosyltransferase Family 58.

Names & Taxonomyi

Protein namesi
Recommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase (EC:2.4.1.258)
Alternative name(s):
Asparagine-linked glycosylation protein 3 homolog
Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase
Not56-like protein
Gene namesi
Name:Alg3
Synonyms:Not, Not56l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:1098592. Alg3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei41 – 6121HelicalSequence AnalysisAdd
BLAST
Transmembranei95 – 11521HelicalSequence AnalysisAdd
BLAST
Transmembranei123 – 14321HelicalSequence AnalysisAdd
BLAST
Transmembranei149 – 16921HelicalSequence AnalysisAdd
BLAST
Transmembranei172 – 19221HelicalSequence AnalysisAdd
BLAST
Transmembranei203 – 22321HelicalSequence AnalysisAdd
BLAST
Transmembranei231 – 25121HelicalSequence AnalysisAdd
BLAST
Transmembranei289 – 30921HelicalSequence AnalysisAdd
BLAST
Transmembranei332 – 35221HelicalSequence AnalysisAdd
BLAST
Transmembranei356 – 37621HelicalSequence AnalysisAdd
BLAST
Transmembranei407 – 42721HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: Ensembl
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 438438Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferasePRO_0000080567Add
BLAST

Proteomic databases

MaxQBiQ8K2A8.
PaxDbiQ8K2A8.
PRIDEiQ8K2A8.

PTM databases

PhosphoSiteiQ8K2A8.

Expressioni

Gene expression databases

BgeeiQ8K2A8.
ExpressionAtlasiQ8K2A8. baseline and differential.
GenevestigatoriQ8K2A8.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045272.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 58 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG241920.
GeneTreeiENSGT00390000013904.
HOGENOMiHOG000237555.
HOVERGENiHBG002070.
InParanoidiQ8K2A8.
KOiK03845.
OMAiAQRWGWG.
OrthoDBiEOG7CVPXS.
TreeFamiTF105870.

Family and domain databases

InterProiIPR007873. Glycosyltransferase_ALG3.
[Graphical view]
PANTHERiPTHR12646. PTHR12646. 1 hit.
PfamiPF05208. ALG3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K2A8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAGLRKRGQ PASVGQPAGI WKQWLQRAWQ ERYLLLREPR YTLLVASCLC
60 70 80 90 100
IAEVGITFWV IHRVAYTEID WKAYMAQVEG FINGTYDYTQ LQGDTGPLVY
110 120 130 140 150
PAGFLYIFTG LFYATDRGTD IPMAQNIFAV LYLVTLVLVF LIYHQTSKVP
160 170 180 190 200
PFVFFFMCCA SYRVHSIFVL RLFNDPVAMA LLFLSINLFL AQCWSWGCCC
210 220 230 240 250
FSLAVSVKMN VLLFAPGLLF LLLTQFGFRG ALPKLAICAA LQVVLGLPFL
260 270 280 290 300
LENPIGYLSR SFDLGRQFLF QWTVNWRFLP ETIFLHRAFH LALLAAHLSL
310 320 330 340 350
LLLFALCRWH RTGESILALL KDPSKRKVPP QALTPNQIVS ILFTSNFIGI
360 370 380 390 400
CFSRSLHYQF YVWYFHTLPY LLWAMPARWL THLLRLLVLG LIELSWNTYP
410 420 430
STSFSSAALH LCHAVVLLQL WLSPESFPKS IQPSRKTH
Length:438
Mass (Da):50,186
Last modified:July 27, 2011 - v2
Checksum:i2332C12F413C897F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti354 – 3541R → H in AAH31893. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC087898 Genomic DNA. No translation available.
BC031893 mRNA. Translation: AAH31893.1.
CCDSiCCDS37289.1.
RefSeqiNP_666051.2. NM_145939.2.
UniGeneiMm.24950.

Genome annotation databases

EnsembliENSMUST00000045918; ENSMUSP00000045272; ENSMUSG00000033809.
GeneIDi208624.
KEGGimmu:208624.
UCSCiuc007yqe.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC087898 Genomic DNA. No translation available.
BC031893 mRNA. Translation: AAH31893.1 .
CCDSi CCDS37289.1.
RefSeqi NP_666051.2. NM_145939.2.
UniGenei Mm.24950.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000045272.

Protein family/group databases

CAZyi GT58. Glycosyltransferase Family 58.

PTM databases

PhosphoSitei Q8K2A8.

Proteomic databases

MaxQBi Q8K2A8.
PaxDbi Q8K2A8.
PRIDEi Q8K2A8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000045918 ; ENSMUSP00000045272 ; ENSMUSG00000033809 .
GeneIDi 208624.
KEGGi mmu:208624.
UCSCi uc007yqe.2. mouse.

Organism-specific databases

CTDi 10195.
MGIi MGI:1098592. Alg3.

Phylogenomic databases

eggNOGi NOG241920.
GeneTreei ENSGT00390000013904.
HOGENOMi HOG000237555.
HOVERGENi HBG002070.
InParanoidi Q8K2A8.
KOi K03845.
OMAi AQRWGWG.
OrthoDBi EOG7CVPXS.
TreeFami TF105870.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_262413. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

NextBioi 372337.
PROi Q8K2A8.
SOURCEi Search...

Gene expression databases

Bgeei Q8K2A8.
ExpressionAtlasi Q8K2A8. baseline and differential.
Genevestigatori Q8K2A8.

Family and domain databases

InterProi IPR007873. Glycosyltransferase_ALG3.
[Graphical view ]
PANTHERi PTHR12646. PTHR12646. 1 hit.
Pfami PF05208. ALG3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiALG3_MOUSE
AccessioniPrimary (citable) accession number: Q8K2A8
Secondary accession number(s): E9QKP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3