Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8K2A8 (ALG3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase

EC=2.4.1.258
Alternative name(s):
Asparagine-linked glycosylation protein 3 homolog
Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase
Not56-like protein
Gene names
Name:Alg3
Synonyms:Not, Not56l
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length438 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc2-PP-Dol.

Catalytic activity

Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Probable.

Sequence similarities

Belongs to the glycosyltransferase 58 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 438438Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
PRO_0000080567

Regions

Transmembrane41 – 6121Helical; Potential
Transmembrane95 – 11521Helical; Potential
Transmembrane123 – 14321Helical; Potential
Transmembrane149 – 16921Helical; Potential
Transmembrane172 – 19221Helical; Potential
Transmembrane203 – 22321Helical; Potential
Transmembrane231 – 25121Helical; Potential
Transmembrane289 – 30921Helical; Potential
Transmembrane332 – 35221Helical; Potential
Transmembrane356 – 37621Helical; Potential
Transmembrane407 – 42721Helical; Potential

Experimental info

Sequence conflict3541R → H in AAH31893. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8K2A8 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 2332C12F413C897F

FASTA43850,186
        10         20         30         40         50         60 
MAAGLRKRGQ PASVGQPAGI WKQWLQRAWQ ERYLLLREPR YTLLVASCLC IAEVGITFWV 

        70         80         90        100        110        120 
IHRVAYTEID WKAYMAQVEG FINGTYDYTQ LQGDTGPLVY PAGFLYIFTG LFYATDRGTD 

       130        140        150        160        170        180 
IPMAQNIFAV LYLVTLVLVF LIYHQTSKVP PFVFFFMCCA SYRVHSIFVL RLFNDPVAMA 

       190        200        210        220        230        240 
LLFLSINLFL AQCWSWGCCC FSLAVSVKMN VLLFAPGLLF LLLTQFGFRG ALPKLAICAA 

       250        260        270        280        290        300 
LQVVLGLPFL LENPIGYLSR SFDLGRQFLF QWTVNWRFLP ETIFLHRAFH LALLAAHLSL 

       310        320        330        340        350        360 
LLLFALCRWH RTGESILALL KDPSKRKVPP QALTPNQIVS ILFTSNFIGI CFSRSLHYQF 

       370        380        390        400        410        420 
YVWYFHTLPY LLWAMPARWL THLLRLLVLG LIELSWNTYP STSFSSAALH LCHAVVLLQL 

       430 
WLSPESFPKS IQPSRKTH 

« Hide

References

[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N-3.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC087898 Genomic DNA. No translation available.
BC031893 mRNA. Translation: AAH31893.1.
CCDSCCDS37289.1.
RefSeqNP_666051.2. NM_145939.2.
UniGeneMm.24950.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000045272.

Protein family/group databases

CAZyGT58. Glycosyltransferase Family 58.

PTM databases

PhosphoSiteQ8K2A8.

Proteomic databases

PaxDbQ8K2A8.
PRIDEQ8K2A8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000045918; ENSMUSP00000045272; ENSMUSG00000033809.
GeneID208624.
KEGGmmu:208624.
UCSCuc007yqe.2. mouse.

Organism-specific databases

CTD10195.
MGIMGI:1098592. Alg3.

Phylogenomic databases

eggNOGNOG241920.
GeneTreeENSGT00390000013904.
HOGENOMHOG000237555.
HOVERGENHBG002070.
InParanoidQ8K2A8.
KOK03845.
OMAAQRWGWG.
OrthoDBEOG7CVPXS.
TreeFamTF105870.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

ArrayExpressQ8K2A8.
BgeeQ8K2A8.
GenevestigatorQ8K2A8.

Family and domain databases

InterProIPR007873. Glycosyltransferase_ALG3.
[Graphical view]
PANTHERPTHR12646. PTHR12646. 1 hit.
PfamPF05208. ALG3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio372337.
PROQ8K2A8.
SOURCESearch...

Entry information

Entry nameALG3_MOUSE
AccessionPrimary (citable) accession number: Q8K2A8
Secondary accession number(s): E9QKP9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot