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Q8K2A8

- ALG3_MOUSE

UniProt

Q8K2A8 - ALG3_MOUSE

Protein

Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase

Gene

Alg3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc2-PP-Dol.

    Catalytic activityi

    Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

    Pathwayi

    GO - Molecular functioni

    1. dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT58. Glycosyltransferase Family 58.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase (EC:2.4.1.258)
    Alternative name(s):
    Asparagine-linked glycosylation protein 3 homolog
    Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
    Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
    Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase
    Not56-like protein
    Gene namesi
    Name:Alg3
    Synonyms:Not, Not56l
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:1098592. Alg3.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 438438Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferasePRO_0000080567Add
    BLAST

    Proteomic databases

    PaxDbiQ8K2A8.
    PRIDEiQ8K2A8.

    PTM databases

    PhosphoSiteiQ8K2A8.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8K2A8.
    BgeeiQ8K2A8.
    GenevestigatoriQ8K2A8.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000045272.

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei41 – 6121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei95 – 11521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei123 – 14321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei149 – 16921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei172 – 19221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei203 – 22321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei231 – 25121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei289 – 30921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei332 – 35221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei356 – 37621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei407 – 42721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 58 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG241920.
    GeneTreeiENSGT00390000013904.
    HOGENOMiHOG000237555.
    HOVERGENiHBG002070.
    InParanoidiQ8K2A8.
    KOiK03845.
    OMAiAQRWGWG.
    OrthoDBiEOG7CVPXS.
    TreeFamiTF105870.

    Family and domain databases

    InterProiIPR007873. Glycosyltransferase_ALG3.
    [Graphical view]
    PANTHERiPTHR12646. PTHR12646. 1 hit.
    PfamiPF05208. ALG3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8K2A8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAGLRKRGQ PASVGQPAGI WKQWLQRAWQ ERYLLLREPR YTLLVASCLC    50
    IAEVGITFWV IHRVAYTEID WKAYMAQVEG FINGTYDYTQ LQGDTGPLVY 100
    PAGFLYIFTG LFYATDRGTD IPMAQNIFAV LYLVTLVLVF LIYHQTSKVP 150
    PFVFFFMCCA SYRVHSIFVL RLFNDPVAMA LLFLSINLFL AQCWSWGCCC 200
    FSLAVSVKMN VLLFAPGLLF LLLTQFGFRG ALPKLAICAA LQVVLGLPFL 250
    LENPIGYLSR SFDLGRQFLF QWTVNWRFLP ETIFLHRAFH LALLAAHLSL 300
    LLLFALCRWH RTGESILALL KDPSKRKVPP QALTPNQIVS ILFTSNFIGI 350
    CFSRSLHYQF YVWYFHTLPY LLWAMPARWL THLLRLLVLG LIELSWNTYP 400
    STSFSSAALH LCHAVVLLQL WLSPESFPKS IQPSRKTH 438
    Length:438
    Mass (Da):50,186
    Last modified:July 27, 2011 - v2
    Checksum:i2332C12F413C897F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti354 – 3541R → H in AAH31893. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC087898 Genomic DNA. No translation available.
    BC031893 mRNA. Translation: AAH31893.1.
    CCDSiCCDS37289.1.
    RefSeqiNP_666051.2. NM_145939.2.
    UniGeneiMm.24950.

    Genome annotation databases

    EnsembliENSMUST00000045918; ENSMUSP00000045272; ENSMUSG00000033809.
    GeneIDi208624.
    KEGGimmu:208624.
    UCSCiuc007yqe.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC087898 Genomic DNA. No translation available.
    BC031893 mRNA. Translation: AAH31893.1 .
    CCDSi CCDS37289.1.
    RefSeqi NP_666051.2. NM_145939.2.
    UniGenei Mm.24950.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000045272.

    Protein family/group databases

    CAZyi GT58. Glycosyltransferase Family 58.

    PTM databases

    PhosphoSitei Q8K2A8.

    Proteomic databases

    PaxDbi Q8K2A8.
    PRIDEi Q8K2A8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000045918 ; ENSMUSP00000045272 ; ENSMUSG00000033809 .
    GeneIDi 208624.
    KEGGi mmu:208624.
    UCSCi uc007yqe.2. mouse.

    Organism-specific databases

    CTDi 10195.
    MGIi MGI:1098592. Alg3.

    Phylogenomic databases

    eggNOGi NOG241920.
    GeneTreei ENSGT00390000013904.
    HOGENOMi HOG000237555.
    HOVERGENi HBG002070.
    InParanoidi Q8K2A8.
    KOi K03845.
    OMAi AQRWGWG.
    OrthoDBi EOG7CVPXS.
    TreeFami TF105870.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 372337.
    PROi Q8K2A8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8K2A8.
    Bgeei Q8K2A8.
    Genevestigatori Q8K2A8.

    Family and domain databases

    InterProi IPR007873. Glycosyltransferase_ALG3.
    [Graphical view ]
    PANTHERi PTHR12646. PTHR12646. 1 hit.
    Pfami PF05208. ALG3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N-3.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiALG3_MOUSE
    AccessioniPrimary (citable) accession number: Q8K2A8
    Secondary accession number(s): E9QKP9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 4, 2005
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3