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Protein

Myotubularin-related protein 3

Gene

Mtmr3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphatase that acts on lipids with a phosphoinositol headgroup. Has phosphatase activity towards phosphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate. May also dephosphorylate proteins phosphorylated on Ser, Thr, and Tyr residues (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotationBy similarity
1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O = 1-phosphatidyl-1D-myo-inositol + phosphate.By similarity
1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei413 – 4131Phosphocysteine intermediatePROSITE-ProRule annotation
Binding sitei459 – 4591SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1117 – 117761FYVE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity Source: UniProtKB
  3. phosphatidylinositol-3-phosphatase activity Source: UniProtKB
  4. protein serine/threonine phosphatase activity Source: MGI
  5. protein tyrosine phosphatase activity Source: MGI

GO - Biological processi

  1. peptidyl-tyrosine dephosphorylation Source: MGI
  2. phosphatidylinositol dephosphorylation Source: UniProtKB
  3. protein dephosphorylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Lipid metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_198973. Synthesis of PIPs at the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Myotubularin-related protein 3 (EC:3.1.3.48)
Alternative name(s):
Phosphatidylinositol-3,5-bisphosphate 3-phosphatase (EC:3.1.3.95By similarity)
Phosphatidylinositol-3-phosphate phosphatase (EC:3.1.3.64By similarity)
Gene namesi
Name:Mtmr3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1921552. Mtmr3.

Subcellular locationi

Cytoplasm By similarity. Membrane By similarity; Peripheral membrane protein By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. extrinsic component of membrane Source: UniProtKB
  3. membrane Source: MGI
  4. nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11961196Myotubularin-related protein 3PRO_0000094937Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei633 – 6331PhosphoserineBy similarity
Modified residuei647 – 6471PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8K296.
PaxDbiQ8K296.
PRIDEiQ8K296.

PTM databases

PhosphoSiteiQ8K296.

Expressioni

Gene expression databases

BgeeiQ8K296.
CleanExiMM_MTMR3.
ExpressionAtlasiQ8K296. baseline and differential.
GenevestigatoriQ8K296.

Structurei

3D structure databases

ProteinModelPortaliQ8K296.
SMRiQ8K296. Positions 48-577, 1059-1177.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini155 – 576422Myotubularin phosphatasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni326 – 3294Substrate bindingBy similarity
Regioni351 – 3522Substrate bindingBy similarity
Regioni413 – 4197Substrate bindingBy similarity

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1027 – 106034Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 1 myotubularin phosphatase domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1117 – 117761FYVE-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiNOG322789.
HOGENOMiHOG000004861.
HOVERGENiHBG052526.
InParanoidiQ8K296.
KOiK18082.
PhylomeDBiQ8K296.
TreeFamiTF315197.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR010569. Myotubularin-like_Pase_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF06602. Myotub-related. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 2 hits.
SSF57903. SSF57903. 1 hit.
PROSITEiPS51339. PPASE_MYOTUBULARIN. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K296-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEEMRHSLE CIQANQIFPR KQLIREDENL QVPFLELHGE STEYVGRAEE
60 70 80 90 100
AIIALSNYRL HIKFKESLVN VPLQLIESVE CRDIFQLHLT CKDCKVIRCQ
110 120 130 140 150
FPTFEQCQDW LKRLNNAIRP PGKIEDLFSF AYHAWCMEVY ASEKEQHGDL
160 170 180 190 200
CRPGEHVTSR FKNEVERMGF DMNNAWRISN INEKYKLCGS YPQELIVPAW
210 220 230 240 250
ITDKELESVA GFRSWKRIPA VIYRHQSNGA VIARCGQPEV SWWGWRNADD
260 270 280 290 300
EHLVQSVARA CASDSQSSIS KVSTRNSCRD FPNAGDLSDV EFDSSLSNTS
310 320 330 340 350
GAESLALQPQ KLLILDARSY AAAVANRAKG GGCECPEYYP NCEVVFMGMA
360 370 380 390 400
NIHSIRRSFQ SLRLLCTQMP DPGNWLSALE STKWLHHLSV LLKSALLVVH
410 420 430 440 450
AVDRDQRPVL VHCSDGWDRT PQIVALAKLL LDPYYRTIEG FQVLVEMEWL
460 470 480 490 500
DFGHKFADRC GHGEDSDDLN ERCPVFLQWL DCVHQLQRQF PCSFEFNEAF
510 520 530 540 550
LVKLVQHTYS CLFGTFLCNN AKERGEKQTQ ERTCSVWSLL RAGNKAFKNL
560 570 580 590 600
LYSSQSEAVL YPVCHVRNLM LWSAVYLPCP SPSTPTDDSC APYPVPGTSP
610 620 630 640 650
DEPPLSRLPK TRSFDNLTTT CENMVPLASR RSSDPSLNEK WQEHGRSLEL
660 670 680 690 700
SSFASAGEEV PAMDSLRKPS RLLGGAELSV AAGVAEGQME NILQEATKEE
710 720 730 740 750
SGVEEPTHRG HTEVPEVKEE APLAKESSMA AEGPVVLYQE PQLDDATLRS
760 770 780 790 800
HQGPSLSLFS QGIPEHQDGH NVLSSSLQAP LRGEDSQEVP VEQPQVENIA
810 820 830 840 850
EDRENVAPAV PVDAKVGLGI SQSSSLLPSQ VPFETRGPHI NNSVHMLLED
860 870 880 890 900
KVKSESGPQL HHRPCPASSG RFSGKDMLPV APEPRSAERP QWDSVLHRTS
910 920 930 940 950
SPGNTLSLLQ APCALPLDKC RQGIVCNGAL ETENKASEQP AGFDTLQKYP
960 970 980 990 1000
TPNGHCANWE AGRSKDSLSH QLSATSCSSA HLYSRNLHHK WLNSHSGRPS
1010 1020 1030 1040 1050
TTSSPDQPSR SHLDDDGMPV YTDTIQQRLR QIESGHQQEV ETLKKQVQEL
1060 1070 1080 1090 1100
KSRLESQYLT SSLRFNGDFG DEVTSIPDSE SNLDQNCVSR CSTEIFSEAS
1110 1120 1130 1140 1150
WEQVDKQDTE MTRWLPDHLA AHCYACDSAF WLASRKHHCR NCGNVFCSSC
1160 1170 1180 1190
CNQKVPVPSQ QLFEPSRVCK SCYSSLHPTS SSIDLELDKP IAATSN
Length:1,196
Mass (Da):133,840
Last modified:May 29, 2007 - v2
Checksum:i409D23ABBEABAC7D
GO
Isoform 2 (identifier: Q8K296-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1076-1196: Missing.

Show »
Length:1,075
Mass (Da):120,297
Checksum:i8BDF16BAC02132E8
GO

Sequence cautioni

The sequence CAI35184.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti782 – 7821R → S in AAH32166 (PubMed:15489334).Curated
Sequence conflicti1074 – 10741T → V in AAH32166 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1076 – 1196121Missing in isoform 2. 1 PublicationVSP_026027Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL645910 Genomic DNA. Translation: CAI35184.1. Sequence problems.
BC032166 mRNA. Translation: AAH32166.1.
RefSeqiXP_006514910.1. XM_006514847.1. [Q8K296-1]
UniGeneiMm.425669.

Genome annotation databases

GeneIDi74302.
KEGGimmu:74302.
UCSCiuc007huy.1. mouse. [Q8K296-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL645910 Genomic DNA. Translation: CAI35184.1. Sequence problems.
BC032166 mRNA. Translation: AAH32166.1.
RefSeqiXP_006514910.1. XM_006514847.1. [Q8K296-1]
UniGeneiMm.425669.

3D structure databases

ProteinModelPortaliQ8K296.
SMRiQ8K296. Positions 48-577, 1059-1177.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ8K296.

Proteomic databases

MaxQBiQ8K296.
PaxDbiQ8K296.
PRIDEiQ8K296.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi74302.
KEGGimmu:74302.
UCSCiuc007huy.1. mouse. [Q8K296-1]

Organism-specific databases

CTDi8897.
MGIiMGI:1921552. Mtmr3.

Phylogenomic databases

eggNOGiNOG322789.
HOGENOMiHOG000004861.
HOVERGENiHBG052526.
InParanoidiQ8K296.
KOiK18082.
PhylomeDBiQ8K296.
TreeFamiTF315197.

Enzyme and pathway databases

ReactomeiREACT_198973. Synthesis of PIPs at the plasma membrane.

Miscellaneous databases

PROiQ8K296.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K296.
CleanExiMM_MTMR3.
ExpressionAtlasiQ8K296. baseline and differential.
GenevestigatoriQ8K296.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR010569. Myotubularin-like_Pase_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF06602. Myotub-related. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 2 hits.
SSF57903. SSF57903. 1 hit.
PROSITEiPS51339. PPASE_MYOTUBULARIN. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiMTMR3_MOUSE
AccessioniPrimary (citable) accession number: Q8K296
Secondary accession number(s): Q5NCA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: May 29, 2007
Last modified: March 4, 2015
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.