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Protein

F-BAR domain only protein 1

Gene

Fcho1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. May regulate Bmp signaling by regulating clathrin-mediated endocytosis of Bmp receptors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
F-BAR domain only protein 1
Gene namesi
Name:Fcho1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1921265. Fcho1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 873873F-BAR domain only protein 1PRO_0000271760Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei295 – 2951PhosphoserineBy similarity
Modified residuei343 – 3431PhosphoserineBy similarity
Modified residuei368 – 3681PhosphoserineBy similarity
Modified residuei518 – 5181PhosphoserineCombined sources
Modified residuei600 – 6001PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8K285.
MaxQBiQ8K285.
PaxDbiQ8K285.
PRIDEiQ8K285.

PTM databases

iPTMnetiQ8K285.
PhosphoSiteiQ8K285.

Expressioni

Tissue specificityi

Mainly detected in brain and spleen.1 Publication

Gene expression databases

BgeeiQ8K285.
CleanExiMM_FCHO1.
ExpressionAtlasiQ8K285. baseline and differential.
GenevisibleiQ8K285. MM.

Interactioni

Subunit structurei

May oligomerize and form homotetramer. Interacts with AP2A2 and AP2B1; 2 subunits of the adaptor protein complex AP-2. Interacts with DAB2 (By similarity). Interacts with clathrin (CLTC or CLTCL1) (By similarity). Interacts with EPS15, EPS15R and ITSN1 (By similarity). Interacts with AGFG1 and CALM (By similarity). May interact with ACVR1; linking this receptor to clathrin-mediated endocytosis (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi216425. 2 interactions.
DIPiDIP-60611N.
STRINGi10090.ENSMUSP00000091151.

Structurei

3D structure databases

ProteinModelPortaliQ8K285.
SMRiQ8K285. Positions 1-269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 248248F-BARPROSITE-ProRule annotationAdd
BLAST
Domaini609 – 872264MHDPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 275275Mediates membrane-bindingBy similarityAdd
BLAST
Regioni267 – 439173Mediates interaction with the adaptor protein complex AP-2By similarityAdd
BLAST
Regioni593 – 873281Mediates interaction with AGFG1, CALM, DAB2, EPS15, EPS15R, ITSN1 and clathrinBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili155 – 17824Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the FCHO family.Curated
Contains 1 F-BAR domain.PROSITE-ProRule annotation
Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IU8F. Eukaryota.
ENOG410ZN1N. LUCA.
GeneTreeiENSGT00510000046419.
HOGENOMiHOG000231544.
HOVERGENiHBG081524.
InParanoidiQ8K285.
KOiK20042.
OMAiGMYLVSC.
OrthoDBiEOG712TVK.
PhylomeDBiQ8K285.
TreeFamiTF328986.

Family and domain databases

InterProiIPR031160. F_BAR.
IPR001060. FCH_dom.
IPR030121. FCHo1.
IPR028565. MHD.
IPR018808. Muniscin_C.
[Graphical view]
PANTHERiPTHR23065:SF6. PTHR23065:SF6. 2 hits.
PfamiPF00611. FCH. 1 hit.
PF10291. muHD. 1 hit.
[Graphical view]
SMARTiSM00055. FCH. 1 hit.
[Graphical view]
PROSITEiPS51741. F_BAR. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K285-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYFGEHFWG DKNHGFEVLY HCVKQGPVAT KELADFIRER ANIEETYSKA
60 70 80 90 100
MAKLSKLASN GTPMGTFAPL WEVFRVSSDK LALCHLELTR KLHDLLKDVL
110 120 130 140 150
RYGEEQLKTH KKCKEEVLGT VDAVQMLSGV GQLLPKSREN YLSRCMDLER
160 170 180 190 200
LRRENTSQKE MDKAETKSKK AADSLRRSVD KYNSARADFE IKMLDSALRF
210 220 230 240 250
QAMEEAHLQH MKALLGSYAH SVEDTHVQIG QVHEEFKQNV ENVTVDMLLR
260 270 280 290 300
KFAESKGTGR EKPGPLDFDA YSSAALQEAM KRLRGAKAFR LPGLSRREPR
310 320 330 340 350
ASVDFLESDS GVPPEVDDEG FTVRPDISQN NGAEPPRFSS SDSDFDDEEP
360 370 380 390 400
RKFYVHIKPA PTRAVACSSE AAAAQLRATA GSLILPPGPG GTMKRHSSRD
410 420 430 440 450
TSGKPQRPRS APRTGSCAEK PLASEEPLSK SLFGPPLESA FDHDDFTGSS
460 470 480 490 500
SLGFTSSPSP FSSSSPENVE DSGLDSPSHA APGPSPESWV PRPGTPQSPP
510 520 530 540 550
TCRAQHPEPR GLMPRAPSPG PWGPEGGADS LTPADPTREG LAATLRRPRS
560 570 580 590 600
RKVSCPLTRS NGDLCRSLSP SPLGSSAPTI PPDRPSFSTQ MGHGISRGPS
610 620 630 640 650
PVVLGSQDAL PVATAFTEYV HAYFRGHSPS CLARVTGELT MTFPAGIVRV
660 670 680 690 700
FSGTPPPPVL SFRLVNTAPV EHFQPNADLI FSDPSQSDPE TKDFWLNMAA
710 720 730 740 750
LTEALQHQAE QNPTASYYNL VLLRYQFSRP GPESVPLQMS AHWQCGPTLT
760 770 780 790 800
RVSVEYSYRA GATAVSTPLT NVQILLPVGE PVTSVRLQPA ATWNTEEKRF
810 820 830 840 850
TWKLPDVCEA GGSGHLSASW QPQSGPSTPS PVAAQFTSEG ATLSGLDLEL
860 870
LGGGYRMSLV KRRFATGMYL VSC
Length:873
Mass (Da):95,122
Last modified:July 27, 2011 - v2
Checksum:iE0D2549EF2A5C020
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti609 – 6091A → T in AAH32207 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC162033 Genomic DNA. No translation available.
BC032207 mRNA. Translation: AAH32207.1.
CCDSiCCDS52588.1.
RefSeqiNP_082991.3. NM_028715.3.
XP_006509828.1. XM_006509765.2.
XP_006509829.1. XM_006509766.2.
XP_006509830.1. XM_006509767.2.
UniGeneiMm.22025.

Genome annotation databases

EnsembliENSMUST00000093444; ENSMUSP00000091151; ENSMUSG00000070000.
ENSMUST00000146100; ENSMUSP00000117606; ENSMUSG00000070000.
GeneIDi74015.
KEGGimmu:74015.
UCSCiuc033jge.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC162033 Genomic DNA. No translation available.
BC032207 mRNA. Translation: AAH32207.1.
CCDSiCCDS52588.1.
RefSeqiNP_082991.3. NM_028715.3.
XP_006509828.1. XM_006509765.2.
XP_006509829.1. XM_006509766.2.
XP_006509830.1. XM_006509767.2.
UniGeneiMm.22025.

3D structure databases

ProteinModelPortaliQ8K285.
SMRiQ8K285. Positions 1-269.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216425. 2 interactions.
DIPiDIP-60611N.
STRINGi10090.ENSMUSP00000091151.

PTM databases

iPTMnetiQ8K285.
PhosphoSiteiQ8K285.

Proteomic databases

EPDiQ8K285.
MaxQBiQ8K285.
PaxDbiQ8K285.
PRIDEiQ8K285.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000093444; ENSMUSP00000091151; ENSMUSG00000070000.
ENSMUST00000146100; ENSMUSP00000117606; ENSMUSG00000070000.
GeneIDi74015.
KEGGimmu:74015.
UCSCiuc033jge.1. mouse.

Organism-specific databases

CTDi23149.
MGIiMGI:1921265. Fcho1.

Phylogenomic databases

eggNOGiENOG410IU8F. Eukaryota.
ENOG410ZN1N. LUCA.
GeneTreeiENSGT00510000046419.
HOGENOMiHOG000231544.
HOVERGENiHBG081524.
InParanoidiQ8K285.
KOiK20042.
OMAiGMYLVSC.
OrthoDBiEOG712TVK.
PhylomeDBiQ8K285.
TreeFamiTF328986.

Miscellaneous databases

PROiQ8K285.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K285.
CleanExiMM_FCHO1.
ExpressionAtlasiQ8K285. baseline and differential.
GenevisibleiQ8K285. MM.

Family and domain databases

InterProiIPR031160. F_BAR.
IPR001060. FCH_dom.
IPR030121. FCHo1.
IPR028565. MHD.
IPR018808. Muniscin_C.
[Graphical view]
PANTHERiPTHR23065:SF6. PTHR23065:SF6. 2 hits.
PfamiPF00611. FCH. 1 hit.
PF10291. muHD. 1 hit.
[Graphical view]
SMARTiSM00055. FCH. 1 hit.
[Graphical view]
PROSITEiPS51741. F_BAR. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-518, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Lung and Spleen.
  4. "Characterization of the EFC/F-BAR domain protein, FCHO2."
    Uezu A., Umeda K., Tsujita K., Suetsugu S., Takenawa T., Nakanishi H.
    Genes Cells 16:868-878(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, OLIGOMERIZATION.
  5. "Distinct and separable activities of the endocytic clathrin-coat components Fcho1/2 and AP-2 in developmental patterning."
    Umasankar P.K., Sanker S., Thieman J.R., Chakraborty S., Wendland B., Tsang M., Traub L.M.
    Nat. Cell Biol. 14:488-501(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AP2A2 AND AP2B1.

Entry informationi

Entry nameiFCHO1_MOUSE
AccessioniPrimary (citable) accession number: Q8K285
Secondary accession number(s): D3Z3Q5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.