Q8K284 (TF3C1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
General transcription factor 3C polypeptide 1

Alternative name(s):
TF3C-alpha
TFIIIC box B-binding subunit
Transcription factor IIIC 220 kDa subunit
Short name=TFIIIC 220 kDa subunit
Short name=TFIIIC220
Transcription factor IIIC subunit alpha

Gene names

Name:

Gtf3c1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	2101 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. Binds to the box B promoter element By similarity.
Subunit structure	Part of the TFIIIC subcomplex TFIIIC2, consisting of six subunits, GTF3C1, GTF3C2, GTF3C3, GTF3C4, GTF3C5 and GTF3C6 By similarity. Interacts with IGHMBP2 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the TFIIIC subunit 1 family.
Sequence caution	The sequence AAH32208.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleolus Inferred from electronic annotation. Source: Compara ribonucleoprotein complex Inferred from direct assay PubMed 19299493. Source: MGI
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8K284-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8K284-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1503-1598: Missing.

Note: Incomplete sequence.

Isoform 3 (identifier: Q8K284-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-156: Missing.
     326-375: TDVMVRCLKL...VFERDMLTQT → KSRTLWPTGS...KRIREVCPEF
     376-2101: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2101	2101	General transcription factor 3C polypeptide 1		PRO_0000209711
Regions
	Compositional bias	344 – 354	11	Asp/Glu-rich (acidic)
	Compositional bias	1199 – 1227	29	Arg/Lys-rich (basic)
	Compositional bias	1607 – 1617	11	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	667	1	Phosphoserine By similarity
	Modified residue	1063	1	Phosphoserine By similarity
	Modified residue	1854	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 156	156	Missing in isoform 3.		VSP_010573
	Alternative sequence	326 – 375	50	TDVMV…MLTQT → KSRTLWPTGSPCCHFLWSHS ALKCEDYTSFSSLLLSAFYL KRIREVCPEF in isoform 3.		VSP_010574
	Alternative sequence	376 – 2101	1726	Missing in isoform 3.		VSP_010575
	Alternative sequence	1503 – 1598	96	Missing in isoform 2.		VSP_010576
Experimental info
	Sequence conflict	1599	1	S → N in AAH32208. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 7, 2004. Version 2. Checksum: 182C2EFFD9599AD6	FASTA	2,101	237,476
	10 20 30 40 50 60 MDALESLLDE VALEGLDGLC LPALWSRLES RSPAFPLPLE PYTQEFLWRA LATHPGISFY 70 80 90 100 110 120 EEPRERPDLQ LQDRYEEIDL ETGILESRRD PVTLEDVYPI HMILENKDGI QGSCRYFKER 130 140 150 160 170 180 KDITSSIRSK CLQPRCTMVE AFSRWGKKLI IVASQDMRYR ALIGLEGDPD LKLPDFSYCI 190 200 210 220 230 240 LERLGRSRWQ GELQRDLHTT AFKVDAGKLH YHRKILNKNG LITMQSHVIR LPTGAQQHSI 250 260 270 280 290 300 LLLLNRFHVD RRSKYDILME KLSMMLSTRS NQIETLGKLR EELGLCERTF KRLYQYMLNA 310 320 330 340 350 360 GLAKVVSLPL QEIHPECGPC KTKKGTDVMV RCLKLLKEFK RKMEDDHDDD DDEEVISKGV 370 380 390 400 410 420 PPVDIVFERD MLTQTYELIE RRGTKGISQA EIRVAMNVGK LEARMLCRLL QRFKVVKGFM 430 440 450 460 470 480 EDEGRQRTTK YISCVFAEES DLSRQYAREK ARGELLTTVS LASVQDESLM PEGEEAFLSD 490 500 510 520 530 540 SESEEESSCS GGKRRGRGSR GHARASGDAG SGSRPHHSTP AKGGWKVLNL HPLKKPKAAA 550 560 570 580 590 600 EERSRRSSAC RDGLDTSSSS ELNAPFDPHS MDSHSGDIAV IEEVRLDNPK EGGGSQKGGR 610 620 630 640 650 660 HGSSQDKPHK TYRLLKRRNL IIEAVTNLRL IESLFTIQKM IMDQEKQEGV STKCCKKSII 670 680 690 700 710 720 RLVRNLSEEG LLRLYRTTVI QDGIKKKVDL VVHPSMDQND PLVRSAIEQV RFRISNSSTA 730 740 750 760 770 780 NRVKVPPAPA PQEEAEEENQ EPEVPSRSAN SDPNTSSKPE STRVKKTDEK MGITPLKNYK 790 800 810 820 830 840 PVIVPGLGRS IGFLPKMPRL KAMHLFLWYL VYGHPAGHTG EQPALHSERK TGKQESSRPG 850 860 870 880 890 900 AQPSSGDDWD TSEAKNNTES SSWESEMELS TEIVYVDEIS WMRYVPPIPI HRDFGFGWAL 910 920 930 940 950 960 VSDILLCLPL SIFVQLVQVS YKVDNLEDFL NDPLKKHTLI RFLPRHIRQQ LLYKRRYIFS 970 980 990 1000 1010 1020 VVENLQRLCY MGLLQFGPTE KFQDKDQVFV FLKKNAVIVD TTICDPHYNL AHSSRPFERR 1030 1040 1050 1060 1070 1080 LYVLDSMQDV ECYWFDLQCI CLNTPLGVVR CPCAQKICPD PGSDPEGSLR KEQESAMDKH 1090 1100 1110 1120 1130 1140 NLERKCAMLE YTTGSREVVD EGLVPGDGLG AAGLDSSFYA HLKRNWVWTS YIINKARKNN 1150 1160 1170 1180 1190 1200 TSENGLTGRL QTFLSKRPMP LGSGGSGRLP LWSEGRADAE LCADKEEQFE LDREPTPGRN 1210 1220 1230 1240 1250 1260 RKVRGGKSQK RKRLKKEPIR KTKRRRRGEH PEAKSKKLRY QDEADQNALR MMTRLRVSWS 1270 1280 1290 1300 1310 1320 MQEDGLLMLC RIASNVLNTK VKGPFVTWQV VRDILHATFE ESLDKTSHSV GRRARYIVKN 1330 1340 1350 1360 1370 1380 PQAFMNYKVC LAEVYQDKAL VGDFMSRKGN YEDPKVCAKE FKEFVEKLKE KFSSGLRNPN 1390 1400 1410 1420 1430 1440 LEIPNTLQEL FAKYRVLAIG DEKDRVRKED ELNSVEDIHF LVLQNLIQST LSLSNSQSNS 1450 1460 1470 1480 1490 1500 CQSFQIFRLY REFREPVLVR AFMECQKRSL VNRRRVSHSQ GPKKNRAVPF VPMSYQLSQS 1510 1520 1530 1540 1550 1560 YYKLFTWRFP TTICTESFQF YDRLRTNGML DQPDHFSFKD LDSSDPSNDL VAFSLDSPGG 1570 1580 1590 1600 1610 1620 HCVTALALFS LGLLSVDVRI PEQIVVVDSS MVESEVMKSL GKDGGLDDDE EEEDLDEGSG 1630 1640 1650 1660 1670 1680 TKRQGVEVKA HQASHTKYLL MRGYYTVPGM VSTRNLNPND SIVVNSCQVK FRLRNTPAST 1690 1700 1710 1720 1730 1740 QLGPTGFTAT PLEELQAGLS CLPASFTSLV DPQLRTHCPE EFAHQMAQSG YSPEDVAASL 1750 1760 1770 1780 1790 1800 EILQAVAAAD CFGIDKEKLS RQFSALEKIA DRRTRTFLDY IQDLLEQEQV MEVGGNTVRL 1810 1820 1830 1840 1850 1860 VTMASAQPWL LHSMRLRDME VDTKASGDDS QSRLPEGPSI EDHTSEGAAV PPVSSHSTKK 1870 1880 1890 1900 1910 1920 RPHCPETDAE EATRLPAKKP TLQDVRVAAS PRPGAEEQAE AQAPAQLAAP EDADAGGPRQ 1930 1940 1950 1960 1970 1980 ENQENVGVSG LEQLGCEFQL PEGSEDPRGL TESNMAQAAW ESGCERVCFV GRPWRGVDGH 1990 2000 2010 2020 2030 2040 LNMPVCKGML EAVLYHIMSR PGVPESCLLQ YYQGVLQPVA VLELLRGLES LGCIQKRTLR 2050 2060 2070 2080 2090 2100 KPASVSLFSR PVVEGLGQAS EAEALSCHES TVTFYEPTLD CTIRLGRVFP HDINWNKWIH L
	Isoform 2 [UniParc]. Checksum: 056891D09CC78140	FASTA	2,005	226,731
	10 20 30 40 50 60 MDALESLLDE VALEGLDGLC LPALWSRLES RSPAFPLPLE PYTQEFLWRA LATHPGISFY 70 80 90 100 110 120 EEPRERPDLQ LQDRYEEIDL ETGILESRRD PVTLEDVYPI HMILENKDGI QGSCRYFKER 130 140 150 160 170 180 KDITSSIRSK CLQPRCTMVE AFSRWGKKLI IVASQDMRYR ALIGLEGDPD LKLPDFSYCI 190 200 210 220 230 240 LERLGRSRWQ GELQRDLHTT AFKVDAGKLH YHRKILNKNG LITMQSHVIR LPTGAQQHSI 250 260 270 280 290 300 LLLLNRFHVD RRSKYDILME KLSMMLSTRS NQIETLGKLR EELGLCERTF KRLYQYMLNA 310 320 330 340 350 360 GLAKVVSLPL QEIHPECGPC KTKKGTDVMV RCLKLLKEFK RKMEDDHDDD DDEEVISKGV 370 380 390 400 410 420 PPVDIVFERD MLTQTYELIE RRGTKGISQA EIRVAMNVGK LEARMLCRLL QRFKVVKGFM 430 440 450 460 470 480 EDEGRQRTTK YISCVFAEES DLSRQYAREK ARGELLTTVS LASVQDESLM PEGEEAFLSD 490 500 510 520 530 540 SESEEESSCS GGKRRGRGSR GHARASGDAG SGSRPHHSTP AKGGWKVLNL HPLKKPKAAA 550 560 570 580 590 600 EERSRRSSAC RDGLDTSSSS ELNAPFDPHS MDSHSGDIAV IEEVRLDNPK EGGGSQKGGR 610 620 630 640 650 660 HGSSQDKPHK TYRLLKRRNL IIEAVTNLRL IESLFTIQKM IMDQEKQEGV STKCCKKSII 670 680 690 700 710 720 RLVRNLSEEG LLRLYRTTVI QDGIKKKVDL VVHPSMDQND PLVRSAIEQV RFRISNSSTA 730 740 750 760 770 780 NRVKVPPAPA PQEEAEEENQ EPEVPSRSAN SDPNTSSKPE STRVKKTDEK MGITPLKNYK 790 800 810 820 830 840 PVIVPGLGRS IGFLPKMPRL KAMHLFLWYL VYGHPAGHTG EQPALHSERK TGKQESSRPG 850 860 870 880 890 900 AQPSSGDDWD TSEAKNNTES SSWESEMELS TEIVYVDEIS WMRYVPPIPI HRDFGFGWAL 910 920 930 940 950 960 VSDILLCLPL SIFVQLVQVS YKVDNLEDFL NDPLKKHTLI RFLPRHIRQQ LLYKRRYIFS 970 980 990 1000 1010 1020 VVENLQRLCY MGLLQFGPTE KFQDKDQVFV FLKKNAVIVD TTICDPHYNL AHSSRPFERR 1030 1040 1050 1060 1070 1080 LYVLDSMQDV ECYWFDLQCI CLNTPLGVVR CPCAQKICPD PGSDPEGSLR KEQESAMDKH 1090 1100 1110 1120 1130 1140 NLERKCAMLE YTTGSREVVD EGLVPGDGLG AAGLDSSFYA HLKRNWVWTS YIINKARKNN 1150 1160 1170 1180 1190 1200 TSENGLTGRL QTFLSKRPMP LGSGGSGRLP LWSEGRADAE LCADKEEQFE LDREPTPGRN 1210 1220 1230 1240 1250 1260 RKVRGGKSQK RKRLKKEPIR KTKRRRRGEH PEAKSKKLRY QDEADQNALR MMTRLRVSWS 1270 1280 1290 1300 1310 1320 MQEDGLLMLC RIASNVLNTK VKGPFVTWQV VRDILHATFE ESLDKTSHSV GRRARYIVKN 1330 1340 1350 1360 1370 1380 PQAFMNYKVC LAEVYQDKAL VGDFMSRKGN YEDPKVCAKE FKEFVEKLKE KFSSGLRNPN 1390 1400 1410 1420 1430 1440 LEIPNTLQEL FAKYRVLAIG DEKDRVRKED ELNSVEDIHF LVLQNLIQST LSLSNSQSNS 1450 1460 1470 1480 1490 1500 CQSFQIFRLY REFREPVLVR AFMECQKRSL VNRRRVSHSQ GPKKNRAVPF VPMSYQLSQS 1510 1520 1530 1540 1550 1560 YYSLGKDGGL DDDEEEEDLD EGSGTKRQGV EVKAHQASHT KYLLMRGYYT VPGMVSTRNL 1570 1580 1590 1600 1610 1620 NPNDSIVVNS CQVKFRLRNT PASTQLGPTG FTATPLEELQ AGLSCLPASF TSLVDPQLRT 1630 1640 1650 1660 1670 1680 HCPEEFAHQM AQSGYSPEDV AASLEILQAV AAADCFGIDK EKLSRQFSAL EKIADRRTRT 1690 1700 1710 1720 1730 1740 FLDYIQDLLE QEQVMEVGGN TVRLVTMASA QPWLLHSMRL RDMEVDTKAS GDDSQSRLPE 1750 1760 1770 1780 1790 1800 GPSIEDHTSE GAAVPPVSSH STKKRPHCPE TDAEEATRLP AKKPTLQDVR VAASPRPGAE 1810 1820 1830 1840 1850 1860 EQAEAQAPAQ LAAPEDADAG GPRQENQENV GVSGLEQLGC EFQLPEGSED PRGLTESNMA 1870 1880 1890 1900 1910 1920 QAAWESGCER VCFVGRPWRG VDGHLNMPVC KGMLEAVLYH IMSRPGVPES CLLQYYQGVL 1930 1940 1950 1960 1970 1980 QPVAVLELLR GLESLGCIQK RTLRKPASVS LFSRPVVEGL GQASEAEALS CHESTVTFYE 1990 2000 PTLDCTIRLG RVFPHDINWN KWIHL
	Isoform 3 [UniParc]. Checksum: A16FC3AA7DE188F1	FASTA	219	25,427
	10 20 30 40 50 60 MRYRALIGLE GDPDLKLPDF SYCILERLGR SRWQGELQRD LHTTAFKVDA GKLHYHRKIL 70 80 90 100 110 120 NKNGLITMQS HVIRLPTGAQ QHSILLLLNR FHVDRRSKYD ILMEKLSMML STRSNQIETL 130 140 150 160 170 180 GKLREELGLC ERTFKRLYQY MLNAGLAKVV SLPLQEIHPE CGPCKTKKGK SRTLWPTGSP 190 200 210 CCHFLWSHSA LKCEDYTSFS SLLLSAFYLK RIREVCPEF

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: C57BL/6J. Tissue: Hypothalamus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6. Tissue: Brain and Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK038537 mRNA. Translation: BAC30032.1. BC032208 mRNA. Translation: AAH32208.1. Different initiation. BC067041 mRNA. Translation: AAH67041.1.
IPI	IPI00403414. IPI00416203. IPI00416204.
RefSeq	NP_997122.1. NM_207239.1.
UniGene	Mm.294173.
3D structure databases
ProteinModelPortal	Q8K284.
ModBase	Search...
PTM databases
PhosphoSite	Q8K284.
Proteomic databases
PaxDb	Q8K284.
PRIDE	Q8K284.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000055506; ENSMUSP00000056719; ENSMUSG00000032777.
GeneID	233863.
KEGG	mmu:233863.
UCSC	uc009jqh.1. mouse. uc009jql.1. mouse. uc012fto.1. mouse.
Organism-specific databases
CTD	2975.
MGI	MGI:107887. Gtf3c1.
Phylogenomic databases
eggNOG	NOG322015.
GeneTree	ENSGT00390000008664.
HOGENOM	HOG000154556.
HOVERGEN	HBG057283.
InParanoid	Q8K284.
KO	K15199.
OMA	VRFRISN.
OrthoDB	EOG40CHG3.
Gene expression databases
ArrayExpress	Q8K284.
Bgee	Q8K284.
CleanEx	MM_GTF3C1.
Genevestigator	Q8K284.
GermOnline	ENSMUSG00000032777. Mus musculus.
Family and domain databases
InterPro	IPR007309. TFIIIC_Bblock-bd. [Graphical view]
Pfam	PF04182. B-block_TFIIIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GTF3C1. mouse.
NextBio	381879.
SOURCE	Search...

Entry information

Entry name

TF3C1_MOUSE

Accession

Primary (citable) accession number: Q8K284
Secondary accession number(s): Q8CAL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	April 3, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program