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Protein

RNA cytidine acetyltransferase

Gene

Nat10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs. Catalyzes the formation of N4-acetylcytidine (ac4C) in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C in serine and leucine tRNAs. Requires the tRNA-binding adapter protein THUMBD1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation (Probable). Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. Acetylates alpha-tubulin, which may affect microtubule stability and cell division (By similarity).UniRule annotationBy similarity

Catalytic activityi

A cytidine in 18S rRNA + ATP + acetyl-CoA + H2O = an N4-acetylcytidine in 18S rRNA + ADP + phosphate + CoA.UniRule annotation
A cytidine in tRNA + ATP + acetyl-CoA + H2O = an N4-acetylcytidine in tRNA + ADP + phosphate + CoA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei470ATPUniRule annotation1
Binding sitei725Acetyl-CoAUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi287 – 296ATPUniRule annotation10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processrRNA processing, tRNA processing
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA cytidine acetyltransferaseUniRule annotation (EC:2.3.1.-UniRule annotation)
Alternative name(s):
18S rRNA cytosine acetyltransferaseUniRule annotation
N-acetyltransferase 10UniRule annotation
Gene namesi
Name:Nat10UniRule annotation
Synonyms:Kiaa1709
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2138939 Nat10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003274801 – 1024RNA cytidine acetyltransferaseAdd BLAST1024

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei426N6-acetyllysineBy similarity1
Modified residuei716PhosphothreonineBy similarity1
Modified residuei934PhosphoserineBy similarity1
Modified residuei984PhosphoserineBy similarity1
Modified residuei987PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8K224
MaxQBiQ8K224
PaxDbiQ8K224
PeptideAtlasiQ8K224
PRIDEiQ8K224

PTM databases

iPTMnetiQ8K224
PhosphoSitePlusiQ8K224

Expressioni

Gene expression databases

BgeeiENSMUSG00000027185
ExpressionAtlasiQ8K224 baseline and differential
GenevisibleiQ8K224 MM

Interactioni

Subunit structurei

Interacts with THUMBD1 (Probable). Interacts with SUN1 (via N-terminus). Also interacts with TERT (By similarity).UniRule annotationBy similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi221159, 6 interactors
IntActiQ8K224, 4 interactors
MINTiQ8K224
STRINGi10090.ENSMUSP00000028608

Structurei

3D structure databases

ProteinModelPortaliQ8K224
SMRiQ8K224
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini558 – 753N-acetyltransferaseUniRule annotationAdd BLAST196

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni629 – 631Acetyl-CoA bindingUniRule annotation3
Regioni636 – 642Acetyl-CoA bindingUniRule annotation7
Regioni702 – 1024Required for localization to the nucleolus and midbodyBy similarityAdd BLAST323

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi448 – 451Poly-Thr4
Compositional biasi989 – 1024Lys-richAdd BLAST36

Sequence similaritiesi

Belongs to the RNA cytidine acetyltransferase family. NAT10 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2036 Eukaryota
COG1444 LUCA
GeneTreeiENSGT00390000009140
HOGENOMiHOG000210833
HOVERGENiHBG052206
InParanoidiQ8K224
KOiK14521
OMAiGDDEEWN
OrthoDBiEOG091G017T
PhylomeDBiQ8K224
TreeFamiTF300601

Family and domain databases

HAMAPiMF_03211 RNA_acetyltr_Nat10, 1 hit
InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR000182 GNAT_dom
IPR007807 Helicase_dom
IPR033688 NAT10
IPR032672 TmcA/NAT10/Kre33
IPR013562 TmcA_N
IPR027992 tRNA_bind_dom
PANTHERiPTHR10925 PTHR10925, 1 hit
PfamiView protein in Pfam
PF08351 DUF1726, 1 hit
PF13718 GNAT_acetyltr_2, 1 hit
PF05127 Helicase_RecD, 1 hit
PF13725 tRNA_bind_2, 1 hit
SUPFAMiSSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS51186 GNAT, 1 hit

Sequencei

Sequence statusi: Complete.

Q8K224-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRKKVDNRI RILIENGVAE RQRSLFVVVG DRGKDQVVIL HHMLSKATVK
60 70 80 90 100
ARPSVLWCYK KELGFSSHRK KRMRQLQKKI KSGTLNLKQD DPFELFVAAT
110 120 130 140 150
NIRYCYYNET HKILGNTFGM CVLQDFEALT PNLLARTVET VEGGGLVVIL
160 170 180 190 200
LRTMNSLKQL YTMTMDVHSR YRTEAHQDVV GRFNERFILS LASCKKCLVI
210 220 230 240 250
DDQLDILPIS SHVASIEALP PQAPDENLSP AALELLELKE SLQDTQPVGV
260 270 280 290 300
LVDCCKTLDQ AKAVLKFIEG ISEKTLRSTV ALTAARGRGK SAALGLAIAG
310 320 330 340 350
AVAFGYSNIF VTSPSPDNLH TLFEFVFKGF DALQYQEHLD YEIVQSLNPE
360 370 380 390 400
FNKAVIRVNV FREHRQTIQY IHPADAVKLG QAELVVIDEA AAIPLPLVKS
410 420 430 440 450
LLGPYLVFMA STINGYEGTG RSLSLKLIQQ LRQQSAQSQV STTAENKTTT
460 470 480 490 500
TARLASARTL HEVSLQESIR YAPGDAVEKW LNDLLCLDCL NITRIVSGCP
510 520 530 540 550
LPEACELYYV NRDTLFCYHK ASEVFLQRLM ALYVASHYKN SPNDLQMLSD
560 570 580 590 600
APAHHLFCLL PPVPPTQNAL PEVLAVVQVC LEGEISRQSI LNSLSRGKKA
610 620 630 640 650
SGDLIPWTVS EQFQDPDFGG LSGGRVVRIA VHPDYQGMGY GSRALQLLQM
660 670 680 690 700
YYEGKFPCLE EKVLETPQEI RTVSSEAVSL LEEVITPRKD LPPLLLKLNE
710 720 730 740 750
RPAERLDYLG VSYGLTPRLL KFWKRAGFVP VYLRQTPNDL TGEHSCIMLK
760 770 780 790 800
TLADEDEAEQ GAWLAAFWKD FRRRFLALLS YQFSTFSPAL SLNIIQNRNV
810 820 830 840 850
AKSALPALGR EHLEALFLPY DLKRLEMYSR NMVDYHLIMD LIPAISRLYF
860 870 880 890 900
LNQLGDLSLS AAQSALLLGI GLQHKSVDQL EKEIELPSGQ LMGLFNRIIR
910 920 930 940 950
KVVKLFNDVQ EKAIEEQMVA VKDVVMEPTM KTLSDDLDEA AKEFQEKHKK
960 970 980 990 1000
EVGKLKDMDL SQYVIRGDDE EWNEVLSKAG QNASIVSLKS DKKRKLETKQ
1010 1020
EPKQSKKLKK RDNNRKDMKL KRKK
Length:1,024
Mass (Da):115,419
Last modified:October 1, 2002 - v1
Checksum:iBB4DE75F6686C894
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75 – 81QLQKKIK → FSHNKIM in BAC35626 (PubMed:16141072).Curated7
Sequence conflicti86N → Y in BAC35626 (PubMed:16141072).Curated1
Sequence conflicti208P → S in BAE29107 (PubMed:16141072).Curated1
Sequence conflicti840D → G in BAE29107 (PubMed:16141072).Curated1
Sequence conflicti865 – 904ALLLG…RKVVK → VGHLGLHGGQGRTVELL in BAC98237 (PubMed:14621295).CuratedAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX537331 Genomic DNA No translation available.
BC034516 mRNA Translation: AAH34516.1
BC047436 mRNA Translation: AAH47436.1
AK054029 mRNA Translation: BAC35626.1
AK149825 mRNA Translation: BAE29107.1
AK129427 Transcribed RNA Translation: BAC98237.1
CCDSiCCDS16480.1
RefSeqiNP_694766.1, NM_153126.2
UniGeneiMm.288005
Mm.490982

Genome annotation databases

EnsembliENSMUST00000028608; ENSMUSP00000028608; ENSMUSG00000027185
GeneIDi98956
KEGGimmu:98956
UCSCiuc008liy.1 mouse

Similar proteinsi

Entry informationi

Entry nameiNAT10_MOUSE
AccessioniPrimary (citable) accession number: Q8K224
Secondary accession number(s): Q3UE04
, Q6ZPJ7, Q80VD3, Q8BW78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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