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Protein

RNA cytidine acetyltransferase

Gene

Nat10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs. Catalyzes the formation of N(4)-acetylcytidine (ac4C) in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C in serine and leucine tRNAs. Requires the tRNA-binding adapter protein THUMBD1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation (Probable). Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. Acetylates alpha-tubulin, which may affect microtubule stability and cell division (By similarity).UniRule annotationBy similarity

Catalytic activityi

A cytidine in 18S rRNA + ATP + acetyl-CoA + H2O = an N(4)-acetylcytidine in 18S rRNA + ADP + phosphate + CoA.UniRule annotation
A cytidine in tRNA + ATP + acetyl-CoA + H2O = an N(4)-acetylcytidine in tRNAA + ADP + phosphate + CoA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei470 – 4701ATPUniRule annotation
Binding sitei725 – 7251Acetyl-CoAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi287 – 29610ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

rRNA processing, tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA cytidine acetyltransferaseUniRule annotation (EC:2.3.1.-UniRule annotation)
Alternative name(s):
18S rRNA cytosine acetyltransferaseUniRule annotation
N-acetyltransferase 10UniRule annotation
Gene namesi
Name:Nat10UniRule annotation
Synonyms:Kiaa1709
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2138939. Nat10.

Subcellular locationi

  • Nucleusnucleolus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10241024RNA cytidine acetyltransferasePRO_0000327480Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei426 – 4261N6-acetyllysineBy similarity
Modified residuei716 – 7161PhosphothreonineBy similarity
Modified residuei934 – 9341PhosphoserineBy similarity
Modified residuei984 – 9841PhosphoserineBy similarity
Modified residuei987 – 9871PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8K224.
MaxQBiQ8K224.
PaxDbiQ8K224.
PeptideAtlasiQ8K224.
PRIDEiQ8K224.

PTM databases

iPTMnetiQ8K224.
PhosphoSiteiQ8K224.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027185.
ExpressionAtlasiQ8K224. baseline and differential.
GenevisibleiQ8K224. MM.

Interactioni

Subunit structurei

Interacts with THUMBD1 (Probable). Interacts with SUN1 (via N-terminus). Also interacts with TERT (By similarity).UniRule annotationBy similarity

Protein-protein interaction databases

BioGridi221159. 3 interactions.
IntActiQ8K224. 2 interactions.
MINTiMINT-4117163.
STRINGi10090.ENSMUSP00000028608.

Structurei

3D structure databases

ProteinModelPortaliQ8K224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini558 – 753196N-acetyltransferaseUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni629 – 6313Acetyl-CoA bindingUniRule annotation
Regioni636 – 6427Acetyl-CoA bindingUniRule annotation
Regioni702 – 1024323Required for localization to the nucleolus and midbodyBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi448 – 4514Poly-Thr
Compositional biasi989 – 102436Lys-richAdd
BLAST

Sequence similaritiesi

Belongs to the RNA cytidine acetyltransferase family. NAT10 subfamily.UniRule annotation
Contains 1 N-acetyltransferase domain.UniRule annotation

Phylogenomic databases

eggNOGiKOG2036. Eukaryota.
COG1444. LUCA.
GeneTreeiENSGT00390000009140.
HOGENOMiHOG000210833.
HOVERGENiHBG052206.
InParanoidiQ8K224.
KOiK14521.
OMAiHYKNTPD.
OrthoDBiEOG091G017T.
PhylomeDBiQ8K224.
TreeFamiTF300601.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
HAMAPiMF_03211. RNA_acetyltr_Nat10. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
IPR007807. Helicase_dom.
IPR033688. NAT10.
IPR032672. TmcA/NAT10/Kre33.
IPR013562. TmcA_N.
IPR027992. tRNA_bind_dom.
[Graphical view]
PANTHERiPTHR10925. PTHR10925. 1 hit.
PfamiPF08351. DUF1726. 1 hit.
PF13718. GNAT_acetyltr_2. 1 hit.
PF05127. Helicase_RecD. 1 hit.
PF13725. tRNA_bind_2. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K224-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRKKVDNRI RILIENGVAE RQRSLFVVVG DRGKDQVVIL HHMLSKATVK
60 70 80 90 100
ARPSVLWCYK KELGFSSHRK KRMRQLQKKI KSGTLNLKQD DPFELFVAAT
110 120 130 140 150
NIRYCYYNET HKILGNTFGM CVLQDFEALT PNLLARTVET VEGGGLVVIL
160 170 180 190 200
LRTMNSLKQL YTMTMDVHSR YRTEAHQDVV GRFNERFILS LASCKKCLVI
210 220 230 240 250
DDQLDILPIS SHVASIEALP PQAPDENLSP AALELLELKE SLQDTQPVGV
260 270 280 290 300
LVDCCKTLDQ AKAVLKFIEG ISEKTLRSTV ALTAARGRGK SAALGLAIAG
310 320 330 340 350
AVAFGYSNIF VTSPSPDNLH TLFEFVFKGF DALQYQEHLD YEIVQSLNPE
360 370 380 390 400
FNKAVIRVNV FREHRQTIQY IHPADAVKLG QAELVVIDEA AAIPLPLVKS
410 420 430 440 450
LLGPYLVFMA STINGYEGTG RSLSLKLIQQ LRQQSAQSQV STTAENKTTT
460 470 480 490 500
TARLASARTL HEVSLQESIR YAPGDAVEKW LNDLLCLDCL NITRIVSGCP
510 520 530 540 550
LPEACELYYV NRDTLFCYHK ASEVFLQRLM ALYVASHYKN SPNDLQMLSD
560 570 580 590 600
APAHHLFCLL PPVPPTQNAL PEVLAVVQVC LEGEISRQSI LNSLSRGKKA
610 620 630 640 650
SGDLIPWTVS EQFQDPDFGG LSGGRVVRIA VHPDYQGMGY GSRALQLLQM
660 670 680 690 700
YYEGKFPCLE EKVLETPQEI RTVSSEAVSL LEEVITPRKD LPPLLLKLNE
710 720 730 740 750
RPAERLDYLG VSYGLTPRLL KFWKRAGFVP VYLRQTPNDL TGEHSCIMLK
760 770 780 790 800
TLADEDEAEQ GAWLAAFWKD FRRRFLALLS YQFSTFSPAL SLNIIQNRNV
810 820 830 840 850
AKSALPALGR EHLEALFLPY DLKRLEMYSR NMVDYHLIMD LIPAISRLYF
860 870 880 890 900
LNQLGDLSLS AAQSALLLGI GLQHKSVDQL EKEIELPSGQ LMGLFNRIIR
910 920 930 940 950
KVVKLFNDVQ EKAIEEQMVA VKDVVMEPTM KTLSDDLDEA AKEFQEKHKK
960 970 980 990 1000
EVGKLKDMDL SQYVIRGDDE EWNEVLSKAG QNASIVSLKS DKKRKLETKQ
1010 1020
EPKQSKKLKK RDNNRKDMKL KRKK
Length:1,024
Mass (Da):115,419
Last modified:October 1, 2002 - v1
Checksum:iBB4DE75F6686C894
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti75 – 817QLQKKIK → FSHNKIM in BAC35626 (PubMed:16141072).Curated
Sequence conflicti86 – 861N → Y in BAC35626 (PubMed:16141072).Curated
Sequence conflicti208 – 2081P → S in BAE29107 (PubMed:16141072).Curated
Sequence conflicti840 – 8401D → G in BAE29107 (PubMed:16141072).Curated
Sequence conflicti865 – 90440ALLLG…RKVVK → VGHLGLHGGQGRTVELL in BAC98237 (PubMed:14621295).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX537331 Genomic DNA. Translation: CAM21435.1.
BC034516 mRNA. Translation: AAH34516.1.
BC047436 mRNA. Translation: AAH47436.1.
AK054029 mRNA. Translation: BAC35626.1.
AK149825 mRNA. Translation: BAE29107.1.
AK129427 Transcribed RNA. Translation: BAC98237.1.
CCDSiCCDS16480.1.
RefSeqiNP_694766.1. NM_153126.2.
UniGeneiMm.288005.
Mm.490982.

Genome annotation databases

EnsembliENSMUST00000028608; ENSMUSP00000028608; ENSMUSG00000027185.
GeneIDi98956.
KEGGimmu:98956.
UCSCiuc008liy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX537331 Genomic DNA. Translation: CAM21435.1.
BC034516 mRNA. Translation: AAH34516.1.
BC047436 mRNA. Translation: AAH47436.1.
AK054029 mRNA. Translation: BAC35626.1.
AK149825 mRNA. Translation: BAE29107.1.
AK129427 Transcribed RNA. Translation: BAC98237.1.
CCDSiCCDS16480.1.
RefSeqiNP_694766.1. NM_153126.2.
UniGeneiMm.288005.
Mm.490982.

3D structure databases

ProteinModelPortaliQ8K224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221159. 3 interactions.
IntActiQ8K224. 2 interactions.
MINTiMINT-4117163.
STRINGi10090.ENSMUSP00000028608.

PTM databases

iPTMnetiQ8K224.
PhosphoSiteiQ8K224.

Proteomic databases

EPDiQ8K224.
MaxQBiQ8K224.
PaxDbiQ8K224.
PeptideAtlasiQ8K224.
PRIDEiQ8K224.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028608; ENSMUSP00000028608; ENSMUSG00000027185.
GeneIDi98956.
KEGGimmu:98956.
UCSCiuc008liy.1. mouse.

Organism-specific databases

CTDi55226.
MGIiMGI:2138939. Nat10.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2036. Eukaryota.
COG1444. LUCA.
GeneTreeiENSGT00390000009140.
HOGENOMiHOG000210833.
HOVERGENiHBG052206.
InParanoidiQ8K224.
KOiK14521.
OMAiHYKNTPD.
OrthoDBiEOG091G017T.
PhylomeDBiQ8K224.
TreeFamiTF300601.

Miscellaneous databases

ChiTaRSiNat10. mouse.
PROiQ8K224.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027185.
ExpressionAtlasiQ8K224. baseline and differential.
GenevisibleiQ8K224. MM.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
HAMAPiMF_03211. RNA_acetyltr_Nat10. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
IPR007807. Helicase_dom.
IPR033688. NAT10.
IPR032672. TmcA/NAT10/Kre33.
IPR013562. TmcA_N.
IPR027992. tRNA_bind_dom.
[Graphical view]
PANTHERiPTHR10925. PTHR10925. 1 hit.
PfamiPF08351. DUF1726. 1 hit.
PF13718. GNAT_acetyltr_2. 1 hit.
PF05127. Helicase_RecD. 1 hit.
PF13725. tRNA_bind_2. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAT10_MOUSE
AccessioniPrimary (citable) accession number: Q8K224
Secondary accession number(s): Q3UE04
, Q6ZPJ7, Q80VD3, Q8BW78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2002
Last modified: September 7, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.