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Protein

Polycomb protein SCMH1

Gene

Scmh1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Associates with Polycomb group (PcG) multiprotein complexes; the complex class is required to maintain the transcriptionally repressive state of some genes.By similarity

GO - Biological processi

  • anterior/posterior pattern specification Source: MGI
  • chromatin remodeling Source: MGI
  • gene silencing Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • spermatogenesis Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-2559580. Oxidative Stress Induced Senescence.
R-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-4570464. SUMOylation of RNA binding proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Polycomb protein SCMH1
Alternative name(s):
Sex comb on midleg homolog 1
Gene namesi
Name:Scmh1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1352762. Scmh1.

Subcellular locationi

GO - Cellular componenti

  • chromocenter Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 706706Polycomb protein SCMH1PRO_0000114335Add
BLAST

Proteomic databases

MaxQBiQ8K214.
PaxDbiQ8K214.
PRIDEiQ8K214.

PTM databases

iPTMnetiQ8K214.
PhosphoSiteiQ8K214.

Expressioni

Tissue specificityi

Most abundant in testis. Moderate levels detected in heart, brain, lung, liver, skeletal muscle and kidney and lower levels in spleen.1 Publication

Developmental stagei

Detected throughout embryogenesis. Expressed ubiquitously in 8.5 dpc embryos. At 10.5 dpc, strongly expressed in nervous system including hindbrain and spinal cord, and in the pharyngeal arches and visceral organs. By 14.5 dpc, strong expression is detected throughout the central nervous system, and in tongue, heart, midgut and urogenital regions.1 Publication

Inductioni

By retinoic acid in F9 and F19 embryonal carcinoma cell lines.1 Publication

Gene expression databases

BgeeiQ8K214.
CleanExiMM_SCMH1.
ExpressionAtlasiQ8K214. baseline and differential.
GenevisibleiQ8K214. MM.

Interactioni

Subunit structurei

Associates with a PRC1-like complex (By similarity). Interacts with the SAM domain of PHC1 via its SAM domain in vitro.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GmnnO885132EBI-445955,EBI-445922
Phc1Q640282EBI-445955,EBI-927346

Protein-protein interaction databases

BioGridi205936. 5 interactions.
DIPiDIP-32567N.
IntActiQ8K214. 4 interactions.
MINTiMINT-1172327.
STRINGi10090.ENSMUSP00000069813.

Structurei

3D structure databases

ProteinModelPortaliQ8K214.
SMRiQ8K214. Positions 27-235, 357-468, 594-659.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati28 – 12699MBT 1Add
BLAST
Repeati134 – 235102MBT 2Add
BLAST
Domaini597 – 66266SAMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the SCM family.Curated
Contains 2 MBT repeats.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IMEZ. Eukaryota.
ENOG410XPKI. LUCA.
GeneTreeiENSGT00760000119024.
HOGENOMiHOG000236280.
HOVERGENiHBG056406.
InParanoidiQ8K214.
KOiK11461.
OMAiAPAHCFK.
OrthoDBiEOG73BVC2.
PhylomeDBiQ8K214.
TreeFamiTF106488.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR021987. DUF3588.
IPR004092. Mbt.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF12140. DUF3588. 1 hit.
PF02820. MBT. 2 hits.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00561. MBT. 2 hits.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS51079. MBT. 2 hits.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1Curated (identifier: Q8K214-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLVCYSVLAC ESLWDLPCSI MGSPLGHFTW DKYLKETCSV PAPVHCFKQS
60 70 80 90 100
YTPPSNEFKI SMKLEAQDPR NTTSTCIATV VGLTGARLRL RLDGSDNKND
110 120 130 140 150
FWRLVDSSEI QPIGNCEKNG GMLQPPLGFR LNASSWPMFL LKTLNGAEMA
160 170 180 190 200
PIKIFHKEPP SPSHNFFKMG MKLEAVDRKN PHFICPATIG EVRGAEVLVT
210 220 230 240 250
FDGWRGAFDY WCRFDSRDIF PVGWCSLTGD NLQPPGTKVV IPKNPSPSSD
260 270 280 290 300
VSTEKPSIHS TKTVLEHQPG QRGRKPGKKR GRTPKILIPH PTSTPSKSAE
310 320 330 340 350
PLKFPKKRGP KPGSKRKPRT LLSPPPTSPT TSTPEPDTST VPQDAATVPS
360 370 380 390 400
SAMQAPTVCI YLNKSGSTGP HLDKKKIQQL PDHFGPARAS VVLQQAVQAC
410 420 430 440 450
IDCAYHQKTV FSFLKQGHGG EVISAVFDRE QHTLNLPAVN SITYVLRFLE
460 470 480 490 500
KLCHNLRSDN LFGNQPFTQT HLSLTATEYN HNHDRYLPGE TFVLGNSLAR
510 520 530 540 550
SLETHSDLMD SALKPANLVS TSQNLRTPGY RPLLPSCGLP LSTVSAVRRL
560 570 580 590 600
CSKGVLKGKK ERRDVESFWK LNHSPGSDRH LESRDPPRLS GRDPSSWTVE
610 620 630 640 650
DVMQFVREAD PQLGSHADLF RKHEIDGKAL LLLRSDMMMK YMGLKLGPAL
660 670 680 690 700
KLSFHIDRLK QVFWKRETIL WSREGLSREV WPISEDTALG HFFSGMDKVF

GSLSKR
Note: No experimental confirmation available.Curated
Length:706
Mass (Da):78,669
Last modified:October 1, 2002 - v1
Checksum:iCC7531B46A439E39
GO
Isoform 21 Publication (identifier: Q8K214-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     662-664: VFW → GKF
     665-706: Missing.

Show »
Length:664
Mass (Da):73,733
Checksum:i642EDBAF598D9179
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei662 – 6643VFW → GKF in isoform 2. 1 PublicationVSP_051680
Alternative sequencei665 – 70642Missing in isoform 2. 1 PublicationVSP_051681Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030906 mRNA. Translation: BAA90554.1.
AL611924 Genomic DNA. Translation: CAM13481.1.
BC034667 mRNA. Translation: AAH34667.1.
CCDSiCCDS18589.1. [Q8K214-2]
CCDS51292.1. [Q8K214-1]
RefSeqiNP_001153102.1. NM_001159630.1. [Q8K214-1]
NP_038911.1. NM_013883.2. [Q8K214-2]
XP_011238853.1. XM_011240551.1. [Q8K214-2]
UniGeneiMm.427014.

Genome annotation databases

EnsembliENSMUST00000000087; ENSMUSP00000000087; ENSMUSG00000000085. [Q8K214-2]
ENSMUST00000064991; ENSMUSP00000069813; ENSMUSG00000000085. [Q8K214-1]
ENSMUST00000106298; ENSMUSP00000101905; ENSMUSG00000000085. [Q8K214-2]
ENSMUST00000106301; ENSMUSP00000101908; ENSMUSG00000000085. [Q8K214-1]
GeneIDi29871.
KEGGimmu:29871.
UCSCiuc008und.2. mouse. [Q8K214-2]
uc008une.2. mouse. [Q8K214-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030906 mRNA. Translation: BAA90554.1.
AL611924 Genomic DNA. Translation: CAM13481.1.
BC034667 mRNA. Translation: AAH34667.1.
CCDSiCCDS18589.1. [Q8K214-2]
CCDS51292.1. [Q8K214-1]
RefSeqiNP_001153102.1. NM_001159630.1. [Q8K214-1]
NP_038911.1. NM_013883.2. [Q8K214-2]
XP_011238853.1. XM_011240551.1. [Q8K214-2]
UniGeneiMm.427014.

3D structure databases

ProteinModelPortaliQ8K214.
SMRiQ8K214. Positions 27-235, 357-468, 594-659.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205936. 5 interactions.
DIPiDIP-32567N.
IntActiQ8K214. 4 interactions.
MINTiMINT-1172327.
STRINGi10090.ENSMUSP00000069813.

PTM databases

iPTMnetiQ8K214.
PhosphoSiteiQ8K214.

Proteomic databases

MaxQBiQ8K214.
PaxDbiQ8K214.
PRIDEiQ8K214.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000087; ENSMUSP00000000087; ENSMUSG00000000085. [Q8K214-2]
ENSMUST00000064991; ENSMUSP00000069813; ENSMUSG00000000085. [Q8K214-1]
ENSMUST00000106298; ENSMUSP00000101905; ENSMUSG00000000085. [Q8K214-2]
ENSMUST00000106301; ENSMUSP00000101908; ENSMUSG00000000085. [Q8K214-1]
GeneIDi29871.
KEGGimmu:29871.
UCSCiuc008und.2. mouse. [Q8K214-2]
uc008une.2. mouse. [Q8K214-1]

Organism-specific databases

CTDi22955.
MGIiMGI:1352762. Scmh1.

Phylogenomic databases

eggNOGiENOG410IMEZ. Eukaryota.
ENOG410XPKI. LUCA.
GeneTreeiENSGT00760000119024.
HOGENOMiHOG000236280.
HOVERGENiHBG056406.
InParanoidiQ8K214.
KOiK11461.
OMAiAPAHCFK.
OrthoDBiEOG73BVC2.
PhylomeDBiQ8K214.
TreeFamiTF106488.

Enzyme and pathway databases

ReactomeiR-MMU-2559580. Oxidative Stress Induced Senescence.
R-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-4570464. SUMOylation of RNA binding proteins.

Miscellaneous databases

ChiTaRSiScmh1. mouse.
PROiQ8K214.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K214.
CleanExiMM_SCMH1.
ExpressionAtlasiQ8K214. baseline and differential.
GenevisibleiQ8K214. MM.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR021987. DUF3588.
IPR004092. Mbt.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF12140. DUF3588. 1 hit.
PF02820. MBT. 2 hits.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00561. MBT. 2 hits.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS51079. MBT. 2 hits.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel member of murine polycomb-group proteins, Sex comb on midleg homolog protein, is highly conserved, and interacts with RAE28/mph1 in vitro."
    Tomotsune D., Takihara Y., Berger J., Duhl D., Joo S., Kyba M., Shirai M., Ohta H., Matsuda Y., Honda B.M., Simon J., Shimada K., Brock H.W., Randazzo F.
    Differentiation 65:229-239(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, INTERACTION WITH PHC1.
    Tissue: Brain1 Publication and Neonatal brain1 Publication.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/NImported.
    Tissue: Mammary glandImported.

Entry informationi

Entry nameiSCMH1_MOUSE
AccessioniPrimary (citable) accession number: Q8K214
Secondary accession number(s): B1AS51, Q9JME0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 1, 2002
Last modified: June 8, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.