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Q8K202

- RPA49_MOUSE

UniProt

Q8K202 - RPA49_MOUSE

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Protein
DNA-directed RNA polymerase I subunit RPA49
Gene
Polr1e, Paf53, Praf1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.2 Publications

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. DNA-directed RNA polymerase activity Source: UniProtKB-KW
  3. RNA polymerase I transcription factor binding Source: MGI
  4. protein binding Source: MGI
Complete GO annotation...

GO - Biological processi

  1. RNA polymerase I transcriptional preinitiation complex assembly at the promoter for the nuclear large rRNA transcript Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transcription

Enzyme and pathway databases

ReactomeiREACT_200667. NoRC negatively regulates rRNA expression.
REACT_214440. NoRC negatively regulates rRNA expression.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase I subunit RPA49
Short name:
RNA polymerase I subunit A49
Alternative name(s):
DNA-directed RNA polymerase I subunit E
RNA polymerase I-associated factor 1
RNA polymerase I-associated factor 53
Gene namesi
Name:Polr1e
Synonyms:Paf53, Praf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1929022. Polr1e.

Subcellular locationi

Nucleusnucleolus 2 Publications

GO - Cellular componenti

  1. DNA-directed RNA polymerase I complex Source: MGI
  2. cell junction Source: Ensembl
  3. cytoplasm Source: Ensembl
  4. nucleolus Source: MGI
  5. nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 482482DNA-directed RNA polymerase I subunit RPA49
PRO_0000073957Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei226 – 2261Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ8K202.

PTM databases

PhosphoSiteiQ8K202.

Expressioni

Gene expression databases

ArrayExpressiQ8K202.
BgeeiQ8K202.
CleanExiMM_POLR1E.
GenevestigatoriQ8K202.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits By similarity. Interacts with PAF49/CD3EAP. Also binds UBTF/UBF.3 Publications

Protein-protein interaction databases

BioGridi211074. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8K202.
SMRiQ8K202. Positions 264-466.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG301234.
GeneTreeiENSGT00390000018004.
HOGENOMiHOG000035116.
HOVERGENiHBG053490.
InParanoidiB1AXQ6.
KOiK03005.
OMAiSGQMEVY.
OrthoDBiEOG73NG39.
PhylomeDBiQ8K202.
TreeFamiTF331685.

Family and domain databases

InterProiIPR009668. RNA_pol-assoc_fac_A49-like.
[Graphical view]
PfamiPF06870. RNA_pol_I_A49. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8K202-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MATLESPGMD DQAGDTETEA LQSARWLYCG EPDDRQKAVL VQFSNGKLQN    50
PGDMRFTLYN STDLVNPRQR SHRIVAAETD RLSYVGNNFG TGALKCNALC 100
RHFVGILNKT SGQMEVYDAE LFNMQPLFAG MGTEVIKLGG QHLYLLAFCQ 150
PSKNLAEAGD LLLSRHRQGH CIAVLLDDDA IEREPPLENQ NKTFRDKLDS 200
CIEAFGSTKQ KRSLNSRRMN KVGSESLNLS VAKAAESIID TKGVNALVSD 250
AMQDDLQDGV LYLPPCYADA AKPEDVYRFE DILSPAEYDA LESPSEAFRK 300
VTSEDILKMI EENSHCSYVI EMLKSLPIDE VHRNRQARSI WFLDALIRFR 350
AQKVIKGKRA LGPGIPHIIN TKLLKQFTCL TYNNGRLQNL ISSSMRAKIT 400
SYAIILALHI NNFQVDLTAL QKDLKLSEKR MIEIAKAMRL KISKQKVSLA 450
DGREESHRLG TLSVPLPPAQ NSDRQSKRRK MN 482
Length:482
Mass (Da):54,034
Last modified:April 26, 2005 - v2
Checksum:i4237E14E1B90BB31
GO
Isoform 2 (identifier: Q8K202-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     130-177: Missing.

Show »
Length:434
Mass (Da):48,864
Checksum:i1E5029FE7C404E68
GO
Isoform 3 (identifier: Q8K202-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     130-177: Missing.
     431-441: MIEIAKAMRLK → PSTSSSSWIWI
     442-482: Missing.

Note: No experimental confirmation available.

Show »
Length:393
Mass (Da):44,212
Checksum:i46ED9B27106CC515
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei130 – 17748Missing in isoform 2 and isoform 3.
VSP_013536Add
BLAST
Alternative sequencei431 – 44111MIEIAKAMRLK → PSTSSSSWIWI in isoform 3.
VSP_013537Add
BLAST
Alternative sequencei442 – 48241Missing in isoform 3.
VSP_013538Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31T → A in AAH34783. 1 Publication
Sequence conflicti258 – 2603DGV → YDA in AAH34783. 1 Publication
Sequence conflicti431 – 4311M → Y in AAH34783. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14336 mRNA. Translation: BAA03266.1.
AK050780 mRNA. Translation: BAC34409.1.
AL824706 Genomic DNA. Translation: CAM17792.1.
AL824706 Genomic DNA. Translation: CAM17794.1.
BC034783 mRNA. Translation: AAH34783.1.
CCDSiCCDS18131.1. [Q8K202-2]
CCDS71378.1. [Q8K202-1]
PIRiS69245.
RefSeqiNP_001272729.1. NM_001285800.1. [Q8K202-1]
NP_073722.1. NM_022811.3. [Q8K202-2]
UniGeneiMm.282932.

Genome annotation databases

EnsembliENSMUST00000029999; ENSMUSP00000029999; ENSMUSG00000028318. [Q8K202-1]
ENSMUST00000107814; ENSMUSP00000103444; ENSMUSG00000028318. [Q8K202-3]
ENSMUST00000133157; ENSMUSP00000121007; ENSMUSG00000028318. [Q8K202-2]
GeneIDi64424.
KEGGimmu:64424.
UCSCiuc012ddk.1. mouse. [Q8K202-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14336 mRNA. Translation: BAA03266.1 .
AK050780 mRNA. Translation: BAC34409.1 .
AL824706 Genomic DNA. Translation: CAM17792.1 .
AL824706 Genomic DNA. Translation: CAM17794.1 .
BC034783 mRNA. Translation: AAH34783.1 .
CCDSi CCDS18131.1. [Q8K202-2 ]
CCDS71378.1. [Q8K202-1 ]
PIRi S69245.
RefSeqi NP_001272729.1. NM_001285800.1. [Q8K202-1 ]
NP_073722.1. NM_022811.3. [Q8K202-2 ]
UniGenei Mm.282932.

3D structure databases

ProteinModelPortali Q8K202.
SMRi Q8K202. Positions 264-466.
ModBasei Search...

Protein-protein interaction databases

BioGridi 211074. 4 interactions.

PTM databases

PhosphoSitei Q8K202.

Proteomic databases

PRIDEi Q8K202.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000029999 ; ENSMUSP00000029999 ; ENSMUSG00000028318 . [Q8K202-1 ]
ENSMUST00000107814 ; ENSMUSP00000103444 ; ENSMUSG00000028318 . [Q8K202-3 ]
ENSMUST00000133157 ; ENSMUSP00000121007 ; ENSMUSG00000028318 . [Q8K202-2 ]
GeneIDi 64424.
KEGGi mmu:64424.
UCSCi uc012ddk.1. mouse. [Q8K202-1 ]

Organism-specific databases

CTDi 64425.
MGIi MGI:1929022. Polr1e.

Phylogenomic databases

eggNOGi NOG301234.
GeneTreei ENSGT00390000018004.
HOGENOMi HOG000035116.
HOVERGENi HBG053490.
InParanoidi B1AXQ6.
KOi K03005.
OMAi SGQMEVY.
OrthoDBi EOG73NG39.
PhylomeDBi Q8K202.
TreeFami TF331685.

Enzyme and pathway databases

Reactomei REACT_200667. NoRC negatively regulates rRNA expression.
REACT_214440. NoRC negatively regulates rRNA expression.

Miscellaneous databases

NextBioi 320075.
PROi Q8K202.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8K202.
Bgeei Q8K202.
CleanExi MM_POLR1E.
Genevestigatori Q8K202.

Family and domain databases

InterProi IPR009668. RNA_pol-assoc_fac_A49-like.
[Graphical view ]
Pfami PF06870. RNA_pol_I_A49. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "RNA polymerase I associated factor 53 binds to the nucleolar transcription factor UBF and functions in specific rDNA transcription."
    Hanada K., Song C.Z., Yamamoto K., Yano K., Maeda Y., Yamaguchi K., Muramatsu M.
    EMBO J. 15:2217-2226(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PROTEIN SEQUENCE OF 110-178 (ISOFORMS 2/3), PROTEIN SEQUENCE OF 222-230 AND 301-305, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH UBTF.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Embryo.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Czech II.
    Tissue: Mammary tumor.
  5. "Constitutive and strong association of PAF53 with RNA polymerase I."
    Seither P., Zatsepina O., Hoffmann M., Grummt I.
    Chromosoma 106:216-225(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE DNA-DIRECTED RNA POLYMERASE I COMPLEX, SUBCELLULAR LOCATION.
  6. "Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription."
    Yamamoto K., Yamamoto M., Hanada K., Nogi Y., Matsuyama T., Muramatsu M.
    Mol. Cell. Biol. 24:6338-6349(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PAF49/CD3EAP.

Entry informationi

Entry nameiRPA49_MOUSE
AccessioniPrimary (citable) accession number: Q8K202
Secondary accession number(s): B1AXQ6
, B1AXQ7, Q62034, Q8BQG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: September 3, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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