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Protein

DNA-directed RNA polymerase I subunit RPA49

Gene

Polr1e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.2 Publications

GO - Molecular functioni

GO - Biological processi

  • RNA polymerase I transcriptional preinitiation complex assembly Source: MGI

Keywordsi

Biological processTranscription

Enzyme and pathway databases

ReactomeiR-MMU-5250924 B-WICH complex positively regulates rRNA expression
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-73772 RNA Polymerase I Promoter Escape
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-73863 RNA Polymerase I Transcription Termination

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase I subunit RPA49
Short name:
RNA polymerase I subunit A49
Alternative name(s):
DNA-directed RNA polymerase I subunit E
RNA polymerase I-associated factor 1
RNA polymerase I-associated factor 53
Gene namesi
Name:Polr1e
Synonyms:Paf53, Praf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1929022 Polr1e

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739571 – 482DNA-directed RNA polymerase I subunit RPA49Add BLAST482

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei226PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PeptideAtlasiQ8K202
PRIDEiQ8K202

PTM databases

iPTMnetiQ8K202
PhosphoSitePlusiQ8K202

Expressioni

Gene expression databases

BgeeiENSMUSG00000028318
CleanExiMM_POLR1E
ExpressionAtlasiQ8K202 baseline and differential
GenevisibleiQ8K202 MM

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits (By similarity). Interacts with PAF49/CD3EAP. Also binds UBTF/UBF.By similarity3 Publications

GO - Molecular functioni

  • RNA polymerase I transcription factor binding Source: MGI

Protein-protein interaction databases

BioGridi211074, 4 interactors

Structurei

3D structure databases

ProteinModelPortaliQ8K202
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000018004
HOGENOMiHOG000035116
HOVERGENiHBG053490
InParanoidiQ8K202
KOiK03005
OMAiRRMNRVG
OrthoDBiEOG091G0YKY
PhylomeDBiQ8K202
TreeFamiTF331685

Family and domain databases

InterProiView protein in InterPro
IPR009668 RNA_pol-assoc_fac_A49-like
PANTHERiPTHR14440 PTHR14440, 1 hit
PfamiView protein in Pfam
PF06870 RNA_pol_I_A49, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K202-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATLESPGMD DQAGDTETEA LQSARWLYCG EPDDRQKAVL VQFSNGKLQN
60 70 80 90 100
PGDMRFTLYN STDLVNPRQR SHRIVAAETD RLSYVGNNFG TGALKCNALC
110 120 130 140 150
RHFVGILNKT SGQMEVYDAE LFNMQPLFAG MGTEVIKLGG QHLYLLAFCQ
160 170 180 190 200
PSKNLAEAGD LLLSRHRQGH CIAVLLDDDA IEREPPLENQ NKTFRDKLDS
210 220 230 240 250
CIEAFGSTKQ KRSLNSRRMN KVGSESLNLS VAKAAESIID TKGVNALVSD
260 270 280 290 300
AMQDDLQDGV LYLPPCYADA AKPEDVYRFE DILSPAEYDA LESPSEAFRK
310 320 330 340 350
VTSEDILKMI EENSHCSYVI EMLKSLPIDE VHRNRQARSI WFLDALIRFR
360 370 380 390 400
AQKVIKGKRA LGPGIPHIIN TKLLKQFTCL TYNNGRLQNL ISSSMRAKIT
410 420 430 440 450
SYAIILALHI NNFQVDLTAL QKDLKLSEKR MIEIAKAMRL KISKQKVSLA
460 470 480
DGREESHRLG TLSVPLPPAQ NSDRQSKRRK MN
Length:482
Mass (Da):54,034
Last modified:April 26, 2005 - v2
Checksum:i4237E14E1B90BB31
GO
Isoform 2 (identifier: Q8K202-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     130-177: Missing.

Show »
Length:434
Mass (Da):48,864
Checksum:i1E5029FE7C404E68
GO
Isoform 3 (identifier: Q8K202-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     130-177: Missing.
     431-441: MIEIAKAMRLK → PSTSSSSWIWI
     442-482: Missing.

Note: No experimental confirmation available.
Show »
Length:393
Mass (Da):44,212
Checksum:i46ED9B27106CC515
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3T → A in AAH34783 (PubMed:15489334).Curated1
Sequence conflicti258 – 260DGV → YDA in AAH34783 (PubMed:15489334).Curated3
Sequence conflicti431M → Y in AAH34783 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013536130 – 177Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST48
Alternative sequenceiVSP_013537431 – 441MIEIAKAMRLK → PSTSSSSWIWI in isoform 3. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_013538442 – 482Missing in isoform 3. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14336 mRNA Translation: BAA03266.1
AK050780 mRNA Translation: BAC34409.1
AL824706 Genomic DNA No translation available.
BC034783 mRNA Translation: AAH34783.1
CCDSiCCDS18131.1 [Q8K202-2]
CCDS71378.1 [Q8K202-1]
PIRiS69245
RefSeqiNP_001272729.1, NM_001285800.1 [Q8K202-1]
NP_073722.1, NM_022811.3 [Q8K202-2]
UniGeneiMm.282932

Genome annotation databases

EnsembliENSMUST00000029999; ENSMUSP00000029999; ENSMUSG00000028318 [Q8K202-1]
ENSMUST00000107814; ENSMUSP00000103444; ENSMUSG00000028318 [Q8K202-3]
ENSMUST00000133157; ENSMUSP00000121007; ENSMUSG00000028318 [Q8K202-2]
GeneIDi64424
KEGGimmu:64424
UCSCiuc012ddk.2 mouse [Q8K202-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRPA49_MOUSE
AccessioniPrimary (citable) accession number: Q8K202
Secondary accession number(s): B1AXQ6
, B1AXQ7, Q62034, Q8BQG9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: May 23, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health