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Q8K202

- RPA49_MOUSE

UniProt

Q8K202 - RPA49_MOUSE

Protein

DNA-directed RNA polymerase I subunit RPA49

Gene

Polr1e

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.2 Publications

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. DNA-directed RNA polymerase activity Source: UniProtKB-KW
    3. protein binding Source: MGI
    4. RNA polymerase I transcription factor binding Source: MGI

    GO - Biological processi

    1. RNA polymerase I transcriptional preinitiation complex assembly at the promoter for the nuclear large rRNA transcript Source: MGI

    Keywords - Biological processi

    Transcription

    Enzyme and pathway databases

    ReactomeiREACT_200667. NoRC negatively regulates rRNA expression.
    REACT_214440. NoRC negatively regulates rRNA expression.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase I subunit RPA49
    Short name:
    RNA polymerase I subunit A49
    Alternative name(s):
    DNA-directed RNA polymerase I subunit E
    RNA polymerase I-associated factor 1
    RNA polymerase I-associated factor 53
    Gene namesi
    Name:Polr1e
    Synonyms:Paf53, Praf1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1929022. Polr1e.

    Subcellular locationi

    Nucleusnucleolus 2 Publications

    GO - Cellular componenti

    1. cell junction Source: Ensembl
    2. cytoplasm Source: Ensembl
    3. DNA-directed RNA polymerase I complex Source: MGI
    4. nucleolus Source: MGI
    5. nucleoplasm Source: Reactome

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 482482DNA-directed RNA polymerase I subunit RPA49PRO_0000073957Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei226 – 2261PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ8K202.

    PTM databases

    PhosphoSiteiQ8K202.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8K202.
    BgeeiQ8K202.
    CleanExiMM_POLR1E.
    GenevestigatoriQ8K202.

    Interactioni

    Subunit structurei

    Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits By similarity. Interacts with PAF49/CD3EAP. Also binds UBTF/UBF.By similarity3 Publications

    Protein-protein interaction databases

    BioGridi211074. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K202.
    SMRiQ8K202. Positions 264-466.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG301234.
    GeneTreeiENSGT00390000018004.
    HOGENOMiHOG000035116.
    HOVERGENiHBG053490.
    InParanoidiB1AXQ6.
    KOiK03005.
    OMAiSGQMEVY.
    OrthoDBiEOG73NG39.
    PhylomeDBiQ8K202.
    TreeFamiTF331685.

    Family and domain databases

    InterProiIPR009668. RNA_pol-assoc_fac_A49-like.
    [Graphical view]
    PfamiPF06870. RNA_pol_I_A49. 2 hits.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8K202-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATLESPGMD DQAGDTETEA LQSARWLYCG EPDDRQKAVL VQFSNGKLQN    50
    PGDMRFTLYN STDLVNPRQR SHRIVAAETD RLSYVGNNFG TGALKCNALC 100
    RHFVGILNKT SGQMEVYDAE LFNMQPLFAG MGTEVIKLGG QHLYLLAFCQ 150
    PSKNLAEAGD LLLSRHRQGH CIAVLLDDDA IEREPPLENQ NKTFRDKLDS 200
    CIEAFGSTKQ KRSLNSRRMN KVGSESLNLS VAKAAESIID TKGVNALVSD 250
    AMQDDLQDGV LYLPPCYADA AKPEDVYRFE DILSPAEYDA LESPSEAFRK 300
    VTSEDILKMI EENSHCSYVI EMLKSLPIDE VHRNRQARSI WFLDALIRFR 350
    AQKVIKGKRA LGPGIPHIIN TKLLKQFTCL TYNNGRLQNL ISSSMRAKIT 400
    SYAIILALHI NNFQVDLTAL QKDLKLSEKR MIEIAKAMRL KISKQKVSLA 450
    DGREESHRLG TLSVPLPPAQ NSDRQSKRRK MN 482
    Length:482
    Mass (Da):54,034
    Last modified:April 26, 2005 - v2
    Checksum:i4237E14E1B90BB31
    GO
    Isoform 2 (identifier: Q8K202-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         130-177: Missing.

    Show »
    Length:434
    Mass (Da):48,864
    Checksum:i1E5029FE7C404E68
    GO
    Isoform 3 (identifier: Q8K202-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         130-177: Missing.
         431-441: MIEIAKAMRLK → PSTSSSSWIWI
         442-482: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:393
    Mass (Da):44,212
    Checksum:i46ED9B27106CC515
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 31T → A in AAH34783. (PubMed:15489334)Curated
    Sequence conflicti258 – 2603DGV → YDA in AAH34783. (PubMed:15489334)Curated
    Sequence conflicti431 – 4311M → Y in AAH34783. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei130 – 17748Missing in isoform 2 and isoform 3. 2 PublicationsVSP_013536Add
    BLAST
    Alternative sequencei431 – 44111MIEIAKAMRLK → PSTSSSSWIWI in isoform 3. 1 PublicationVSP_013537Add
    BLAST
    Alternative sequencei442 – 48241Missing in isoform 3. 1 PublicationVSP_013538Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14336 mRNA. Translation: BAA03266.1.
    AK050780 mRNA. Translation: BAC34409.1.
    AL824706 Genomic DNA. Translation: CAM17792.1.
    AL824706 Genomic DNA. Translation: CAM17794.1.
    BC034783 mRNA. Translation: AAH34783.1.
    CCDSiCCDS18131.1. [Q8K202-2]
    CCDS71378.1. [Q8K202-1]
    PIRiS69245.
    RefSeqiNP_001272729.1. NM_001285800.1. [Q8K202-1]
    NP_073722.1. NM_022811.3. [Q8K202-2]
    UniGeneiMm.282932.

    Genome annotation databases

    EnsembliENSMUST00000029999; ENSMUSP00000029999; ENSMUSG00000028318. [Q8K202-1]
    ENSMUST00000107814; ENSMUSP00000103444; ENSMUSG00000028318. [Q8K202-3]
    ENSMUST00000133157; ENSMUSP00000121007; ENSMUSG00000028318. [Q8K202-2]
    GeneIDi64424.
    KEGGimmu:64424.
    UCSCiuc012ddk.1. mouse. [Q8K202-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14336 mRNA. Translation: BAA03266.1 .
    AK050780 mRNA. Translation: BAC34409.1 .
    AL824706 Genomic DNA. Translation: CAM17792.1 .
    AL824706 Genomic DNA. Translation: CAM17794.1 .
    BC034783 mRNA. Translation: AAH34783.1 .
    CCDSi CCDS18131.1. [Q8K202-2 ]
    CCDS71378.1. [Q8K202-1 ]
    PIRi S69245.
    RefSeqi NP_001272729.1. NM_001285800.1. [Q8K202-1 ]
    NP_073722.1. NM_022811.3. [Q8K202-2 ]
    UniGenei Mm.282932.

    3D structure databases

    ProteinModelPortali Q8K202.
    SMRi Q8K202. Positions 264-466.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 211074. 4 interactions.

    PTM databases

    PhosphoSitei Q8K202.

    Proteomic databases

    PRIDEi Q8K202.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029999 ; ENSMUSP00000029999 ; ENSMUSG00000028318 . [Q8K202-1 ]
    ENSMUST00000107814 ; ENSMUSP00000103444 ; ENSMUSG00000028318 . [Q8K202-3 ]
    ENSMUST00000133157 ; ENSMUSP00000121007 ; ENSMUSG00000028318 . [Q8K202-2 ]
    GeneIDi 64424.
    KEGGi mmu:64424.
    UCSCi uc012ddk.1. mouse. [Q8K202-1 ]

    Organism-specific databases

    CTDi 64425.
    MGIi MGI:1929022. Polr1e.

    Phylogenomic databases

    eggNOGi NOG301234.
    GeneTreei ENSGT00390000018004.
    HOGENOMi HOG000035116.
    HOVERGENi HBG053490.
    InParanoidi B1AXQ6.
    KOi K03005.
    OMAi SGQMEVY.
    OrthoDBi EOG73NG39.
    PhylomeDBi Q8K202.
    TreeFami TF331685.

    Enzyme and pathway databases

    Reactomei REACT_200667. NoRC negatively regulates rRNA expression.
    REACT_214440. NoRC negatively regulates rRNA expression.

    Miscellaneous databases

    NextBioi 320075.
    PROi Q8K202.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8K202.
    Bgeei Q8K202.
    CleanExi MM_POLR1E.
    Genevestigatori Q8K202.

    Family and domain databases

    InterProi IPR009668. RNA_pol-assoc_fac_A49-like.
    [Graphical view ]
    Pfami PF06870. RNA_pol_I_A49. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "RNA polymerase I associated factor 53 binds to the nucleolar transcription factor UBF and functions in specific rDNA transcription."
      Hanada K., Song C.Z., Yamamoto K., Yano K., Maeda Y., Yamaguchi K., Muramatsu M.
      EMBO J. 15:2217-2226(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PROTEIN SEQUENCE OF 110-178 (ISOFORMS 2/3), PROTEIN SEQUENCE OF 222-230 AND 301-305, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH UBTF.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Strain: C57BL/6J.
      Tissue: Embryo.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: Czech II.
      Tissue: Mammary tumor.
    5. "Constitutive and strong association of PAF53 with RNA polymerase I."
      Seither P., Zatsepina O., Hoffmann M., Grummt I.
      Chromosoma 106:216-225(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE DNA-DIRECTED RNA POLYMERASE I COMPLEX, SUBCELLULAR LOCATION.
    6. "Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription."
      Yamamoto K., Yamamoto M., Hanada K., Nogi Y., Matsuyama T., Muramatsu M.
      Mol. Cell. Biol. 24:6338-6349(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PAF49/CD3EAP.

    Entry informationi

    Entry nameiRPA49_MOUSE
    AccessioniPrimary (citable) accession number: Q8K202
    Secondary accession number(s): B1AXQ6
    , B1AXQ7, Q62034, Q8BQG9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3