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Protein

N-arachidonyl glycine receptor

Gene

Gpr18

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for endocannabinoid N-arachidonyl glycine (NAGly) (By similarity). However, conflicting results about the role of NAGly as an agonist are reported (PubMed:23104136). Can also be activated by plant-derived and synthetic cannabinoid agonists (By similarity). The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase (By similarity). May contribute to regulation of the immune system (By similarity). Is required for normal homeostasis of CD8+ subsets of intraepithelial lymphocytes (IELs) (CD8alphaalpha and CD8alphabeta IELs) in small intstine by supporting preferential migration of CD8alphaalpha T-cells to intraepithelial compartment over lamina propria compartment, and by mediating their reconstitution into small intestine after bone marrow transplant (PubMed:25348153, PubMed:26197390). Plays a role in hypotensive responses, mediating reduction in intraocular and blood pressure (PubMed:23461720, PubMed:27893106). Mediates NAGly-induced process of reorganization of actin filaments and induction of acrosomal exocytosis (By similarity).By similarity5 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-373076 Class A/1 (Rhodopsin-like receptors)
R-MMU-418594 G alpha (i) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
N-arachidonyl glycine receptor
Short name:
NAGly receptor
Alternative name(s):
G-protein coupled receptor 18
Gene namesi
Name:Gpr18Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:107859 Gpr18

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 26ExtracellularSequence analysisAdd BLAST26
Transmembranei27 – 47Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini48 – 56CytoplasmicSequence analysis9
Transmembranei57 – 77Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini78 – 95ExtracellularSequence analysisAdd BLAST18
Transmembranei96 – 116Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini117 – 138CytoplasmicSequence analysisAdd BLAST22
Transmembranei139 – 159Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini160 – 191ExtracellularSequence analysisAdd BLAST32
Transmembranei192 – 212Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini213 – 236CytoplasmicSequence analysisAdd BLAST24
Transmembranei237 – 257Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini258 – 268ExtracellularSequence analysisAdd BLAST11
Transmembranei269 – 289Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini290 – 331CytoplasmicSequence analysisAdd BLAST42

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype (PubMed:26197390). However, reduced number of CD8alphaalpha gammadeltaT IELs has been reported (PubMed:25348153).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002781741 – 331N-arachidonyl glycine receptorAdd BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi14N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi94 ↔ 172PROSITE-ProRule annotation
Glycosylationi188N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei322PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8K1Z6
PRIDEiQ8K1Z6

PTM databases

iPTMnetiQ8K1Z6
PhosphoSitePlusiQ8K1Z6

Expressioni

Tissue specificityi

Expressed in the eye including cornea, retina, iris and ciliary epithelium (at protein level) (PubMed:23461720). Expressed in spleen, liver and lymphocytes with highest expression levels in intestinal intraepithelial lymphocytes (PubMed:25348153, PubMed:26197390).3 Publications

Gene expression databases

BgeeiENSMUSG00000050350
CleanExiMM_GPR18
GenevisibleiQ8K1Z6 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000061410

Structurei

3D structure databases

ProteinModelPortaliQ8K1Z6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFPC Eukaryota
ENOG410YP7X LUCA
GeneTreeiENSGT00920000149026
HOGENOMiHOG000112785
HOVERGENiHBG105013
InParanoidiQ8K1Z6
KOiK08410
OMAiSLPFRMF
OrthoDBiEOG091G0BF1
PhylomeDBiQ8K1Z6
TreeFamiTF330775

Family and domain databases

InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR028335 GPR18
PANTHERiPTHR24232:SF1 PTHR24232:SF1, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8K1Z6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLSNHNQL DLSNGSHPEE YKIAALVFYS CIFLIGLFVN VTALWVFSCT
60 70 80 90 100
TKKRTTVTIY MMNVALLDLV FILSLPFRMF YYAKGEWPFG EYFCHILGAL
110 120 130 140 150
VVFYPSLALW LLAFISADRY MAIVQPKYAK ELKNTGKAVL ACGGVWVMTL
160 170 180 190 200
TTTVPLLLLY EDPDKASSPA TCLKISDITH LKAVNVLNFT RLIFFFLIPL
210 220 230 240 250
FIMIGCYVVI IHSLLRGQTS KLKPKVKEKS IRIIMTLLLQ VLVCFVPFHI
260 270 280 290 300
CFAVLMLQGQ ENSYSPWGAF TTFLMNLSTC LDVVLYYIVS KQFQARVISV
310 320 330
MLYRNYLRSV RRKSVRSGSL RSLSNMNSEM L
Length:331
Mass (Da):37,691
Last modified:October 1, 2002 - v1
Checksum:i702FD55C911B4787
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23I → V in BAE42987 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK156039 mRNA Translation: BAE33557.1
AK156868 mRNA Translation: BAE33880.1
AK157029 mRNA Translation: BAE33938.1
AK172401 mRNA Translation: BAE42987.1
BC034872 mRNA Translation: AAH34872.1
CCDSiCCDS27342.1
RefSeqiNP_877958.1, NM_182806.1
UniGeneiMm.37405

Genome annotation databases

EnsembliENSMUST00000055475; ENSMUSP00000061410; ENSMUSG00000050350
GeneIDi110168
KEGGimmu:110168
UCSCiuc007vap.1 mouse

Similar proteinsi

Entry informationi

Entry nameiGPR18_MOUSE
AccessioniPrimary (citable) accession number: Q8K1Z6
Secondary accession number(s): Q3T9N3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 1, 2002
Last modified: June 20, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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