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Q8K1Y2 (KPCD3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase D3

EC=2.7.11.13
Alternative name(s):
Protein kinase C nu type
nPKC-nu
Gene names
Name:Prkd3
Synonyms:Prkcn
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length889 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains 1 and 2 bind both DAG and phorbol ester with high affinity and mediate translocation to the cell membrane. Autophosphorylation of Ser-734 and phosphorylation of Ser-730 by PKC relieves auto-inhibition by the PH domain.

Subcellular location

Cytoplasm By similarity. Membrane By similarity. Note: Translocation to the cell membrane is required for kinase activation By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.

Contains 1 PH domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 889889Serine/threonine-protein kinase D3
PRO_0000055718

Regions

Domain416 – 532117PH
Domain575 – 831257Protein kinase
Zinc finger154 – 20451Phorbol-ester/DAG-type 1
Zinc finger271 – 32151Phorbol-ester/DAG-type 2
Nucleotide binding581 – 5899ATP By similarity

Sites

Active site6981Proton acceptor By similarity
Binding site6041ATP By similarity

Amino acid modifications

Modified residue61Phosphoserine By similarity
Modified residue271Phosphoserine By similarity
Modified residue371Phosphoserine By similarity
Modified residue411Phosphoserine By similarity
Modified residue441Phosphoserine By similarity
Modified residue2131Phosphoserine Ref.2
Modified residue2161Phosphoserine Ref.2
Modified residue3641Phosphoserine By similarity
Modified residue5351Phosphothreonine By similarity
Modified residue7301Phosphoserine; by PKC By similarity
Modified residue7341Phosphoserine; by autocatalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K1Y2 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 7D03938ED4ECC2E4

FASTA889100,078
        10         20         30         40         50         60 
MSANNSPPSA QKSVFPATVS AVLPAPSPCS SPKTGLSARL SNGSFSAPSL TNSRGSVHTV 

        70         80         90        100        110        120 
SFLLQIGLTR ESVTIEAQEL SLSAVKDLVC SIVYQKFPEC GFFGMYDKIL LFRHDMNSEN 

       130        140        150        160        170        180 
ILQLITSADE IHEGDLVEVV LSALATVEDF QIRPHALYVH SYKAPTFCDY CGEMLWGLVR 

       190        200        210        220        230        240 
QGLKCEGCGL NYHKRCAFKI PNNCSGVRKR RLSNVSLPGP GLSVPRPLQP ECVPLLSEES 

       250        260        270        280        290        300 
HTHQEPSKRI PSWSGRPIWM EKMVMCRVKV PHTFAVHSYG RPTICQYCKR LLKGLFRQGM 

       310        320        330        340        350        360 
QCKDCKFNCH KRCASKVPRD CLGEVTFNGE PCSVGTDADM PMDIDSSDVN SDGSRGLDDS 

       370        380        390        400        410        420 
EEPSPPEDKM FFLDPTDLDV ERDEETVKTI SPSTSNNIPL MRVVQSIKHT KRRSSTVVKE 

       430        440        450        460        470        480 
GWMVHYTSRD NLRKRHYWRL DSKCLTLFQN ESGSKYYKEI PLSEILRVSS PQDFTSISQG 

       490        500        510        520        530        540 
SNPHCFEIIT DTVVYFVGEN NGSSSHNPVL AATGVGLDVA QSWEKAIRQA LMPVTPQASV 

       550        560        570        580        590        600 
CTSPGQGKDH NLATSISVSN CQVQENVDIS SVYQIFADEV LGSGQFGIVY GGKHRKTGRD 

       610        620        630        640        650        660 
VAIKVIDKMR FPTKQESQLR NEVAILQNLH HPGIVNLECM FETPERVFVV MEKLHGDMLE 

       670        680        690        700        710        720 
MILSSEKSRL PERITKFMVT QILVALRNLH FKNIVHCDLK PENVLLASAE PFPQVKLCDF 

       730        740        750        760        770        780 
GFARIIGEKS FRRSVVGTPA YLAPEVLRSK GYNRSLDMWS VGVIVYVSLS GTFPFNEDED 

       790        800        810        820        830        840 
INDQIQNAAF MYPPNPWREI SSEAIDLINN LLQVKMRKRY SVDKSLSHPW LQDYQTWLDL 

       850        860        870        880 
REFETRIGER YITHESDDAR WEIHAYTHNL EYPKHFIMAP NPDDMEEDP 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[2]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213 AND SER-216, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC037012 mRNA. Translation: AAH37012.1.
RefSeqNP_083515.2. NM_029239.3.
UniGeneMm.252776.

3D structure databases

ProteinModelPortalQ8K1Y2.
SMRQ8K1Y2. Positions 155-204, 272-321, 414-532, 539-884.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8K1Y2.

Proteomic databases

PaxDbQ8K1Y2.
PRIDEQ8K1Y2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000003191; ENSMUSP00000003191; ENSMUSG00000024070.
ENSMUST00000168887; ENSMUSP00000132004; ENSMUSG00000024070.
GeneID75292.
KEGGmmu:75292.
UCSCuc008dpr.2. mouse.

Organism-specific databases

CTD23683.
MGIMGI:1922542. Prkd3.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00750000117523.
HOGENOMHOG000015135.
HOVERGENHBG003564.
KOK06070.
OrthoDBEOG75B84N.
PhylomeDBQ8K1Y2.

Gene expression databases

ArrayExpressQ8K1Y2.
BgeeQ8K1Y2.
CleanExMM_PRKD3.
GenevestigatorQ8K1Y2.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR015727. Protein_Kinase_C_mu-related.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERPTHR22968. PTHR22968. 1 hit.
PfamPF00130. C1_1. 2 hits.
PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFPIRSF000552. PKC_mu_nu_D2. 1 hit.
PRINTSPR00008. DAGPEDOMAIN.
SMARTSM00109. C1. 2 hits.
SM00233. PH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio342658.
PROQ8K1Y2.
SOURCESearch...

Entry information

Entry nameKPCD3_MOUSE
AccessionPrimary (citable) accession number: Q8K1Y2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot