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Protein

Serine/threonine-protein kinase D3

Gene

Prkd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains 1 and 2 bind both DAG and phorbol ester with high affinity and mediate translocation to the cell membrane. Autophosphorylation of Ser-734 and phosphorylation of Ser-730 by PKC relieves auto-inhibition by the PH domain.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei604 – 6041ATPPROSITE-ProRule annotation
Active sitei698 – 6981Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri154 – 20451Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri271 – 32151Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi581 – 5899ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase D3 (EC:2.7.11.13)
Alternative name(s):
Protein kinase C nu type
nPKC-nu
Gene namesi
Name:Prkd3
Synonyms:Prkcn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1922542. Prkd3.

Subcellular locationi

  • Cytoplasm By similarity
  • Membrane By similarity

  • Note: Translocation to the cell membrane is required for kinase activation.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 889889Serine/threonine-protein kinase D3PRO_0000055718Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei6 – 61PhosphoserineBy similarity
Modified residuei27 – 271PhosphoserineBy similarity
Modified residuei37 – 371PhosphoserineBy similarity
Modified residuei41 – 411PhosphoserineBy similarity
Modified residuei44 – 441PhosphoserineBy similarity
Modified residuei213 – 2131Phosphoserine1 Publication
Modified residuei216 – 2161Phosphoserine1 Publication
Modified residuei346 – 3461PhosphoserineBy similarity
Modified residuei364 – 3641PhosphoserineBy similarity
Modified residuei391 – 3911PhosphoserineBy similarity
Modified residuei395 – 3951PhosphoserineBy similarity
Modified residuei426 – 4261PhosphotyrosineBy similarity
Modified residuei442 – 4421PhosphoserineBy similarity
Modified residuei457 – 4571PhosphotyrosineBy similarity
Modified residuei535 – 5351PhosphothreonineBy similarity
Modified residuei730 – 7301Phosphoserine; by PKCBy similarity
Modified residuei734 – 7341Phosphoserine; by autocatalysisBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8K1Y2.
PaxDbiQ8K1Y2.
PRIDEiQ8K1Y2.

PTM databases

PhosphoSiteiQ8K1Y2.

Expressioni

Gene expression databases

BgeeiQ8K1Y2.
CleanExiMM_PRKD3.
ExpressionAtlasiQ8K1Y2. baseline and differential.
GenevestigatoriQ8K1Y2.

Structurei

3D structure databases

ProteinModelPortaliQ8K1Y2.
SMRiQ8K1Y2. Positions 155-204, 272-321, 414-532, 539-848.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini416 – 532117PHPROSITE-ProRule annotationAdd
BLAST
Domaini575 – 831257Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri154 – 20451Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri271 – 32151Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00780000121901.
HOGENOMiHOG000015135.
HOVERGENiHBG003564.
InParanoidiQ8K1Y2.
KOiK06070.
OrthoDBiEOG75B84N.
PhylomeDBiQ8K1Y2.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR015727. Protein_Kinase_C_mu-related.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR22968. PTHR22968. 1 hit.
PfamiPF00130. C1_1. 2 hits.
PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000552. PKC_mu_nu_D2. 1 hit.
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00233. PH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K1Y2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSANNSPPSA QKSVFPATVS AVLPAPSPCS SPKTGLSARL SNGSFSAPSL
60 70 80 90 100
TNSRGSVHTV SFLLQIGLTR ESVTIEAQEL SLSAVKDLVC SIVYQKFPEC
110 120 130 140 150
GFFGMYDKIL LFRHDMNSEN ILQLITSADE IHEGDLVEVV LSALATVEDF
160 170 180 190 200
QIRPHALYVH SYKAPTFCDY CGEMLWGLVR QGLKCEGCGL NYHKRCAFKI
210 220 230 240 250
PNNCSGVRKR RLSNVSLPGP GLSVPRPLQP ECVPLLSEES HTHQEPSKRI
260 270 280 290 300
PSWSGRPIWM EKMVMCRVKV PHTFAVHSYG RPTICQYCKR LLKGLFRQGM
310 320 330 340 350
QCKDCKFNCH KRCASKVPRD CLGEVTFNGE PCSVGTDADM PMDIDSSDVN
360 370 380 390 400
SDGSRGLDDS EEPSPPEDKM FFLDPTDLDV ERDEETVKTI SPSTSNNIPL
410 420 430 440 450
MRVVQSIKHT KRRSSTVVKE GWMVHYTSRD NLRKRHYWRL DSKCLTLFQN
460 470 480 490 500
ESGSKYYKEI PLSEILRVSS PQDFTSISQG SNPHCFEIIT DTVVYFVGEN
510 520 530 540 550
NGSSSHNPVL AATGVGLDVA QSWEKAIRQA LMPVTPQASV CTSPGQGKDH
560 570 580 590 600
NLATSISVSN CQVQENVDIS SVYQIFADEV LGSGQFGIVY GGKHRKTGRD
610 620 630 640 650
VAIKVIDKMR FPTKQESQLR NEVAILQNLH HPGIVNLECM FETPERVFVV
660 670 680 690 700
MEKLHGDMLE MILSSEKSRL PERITKFMVT QILVALRNLH FKNIVHCDLK
710 720 730 740 750
PENVLLASAE PFPQVKLCDF GFARIIGEKS FRRSVVGTPA YLAPEVLRSK
760 770 780 790 800
GYNRSLDMWS VGVIVYVSLS GTFPFNEDED INDQIQNAAF MYPPNPWREI
810 820 830 840 850
SSEAIDLINN LLQVKMRKRY SVDKSLSHPW LQDYQTWLDL REFETRIGER
860 870 880
YITHESDDAR WEIHAYTHNL EYPKHFIMAP NPDDMEEDP
Length:889
Mass (Da):100,078
Last modified:October 1, 2002 - v1
Checksum:i7D03938ED4ECC2E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC037012 mRNA. Translation: AAH37012.1.
CCDSiCCDS37699.1.
RefSeqiNP_083515.2. NM_029239.3.
UniGeneiMm.252776.

Genome annotation databases

EnsembliENSMUST00000003191; ENSMUSP00000003191; ENSMUSG00000024070.
ENSMUST00000168887; ENSMUSP00000132004; ENSMUSG00000024070.
GeneIDi75292.
KEGGimmu:75292.
UCSCiuc008dpr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC037012 mRNA. Translation: AAH37012.1.
CCDSiCCDS37699.1.
RefSeqiNP_083515.2. NM_029239.3.
UniGeneiMm.252776.

3D structure databases

ProteinModelPortaliQ8K1Y2.
SMRiQ8K1Y2. Positions 155-204, 272-321, 414-532, 539-848.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ8K1Y2.

Proteomic databases

MaxQBiQ8K1Y2.
PaxDbiQ8K1Y2.
PRIDEiQ8K1Y2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003191; ENSMUSP00000003191; ENSMUSG00000024070.
ENSMUST00000168887; ENSMUSP00000132004; ENSMUSG00000024070.
GeneIDi75292.
KEGGimmu:75292.
UCSCiuc008dpr.2. mouse.

Organism-specific databases

CTDi23683.
MGIiMGI:1922542. Prkd3.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00780000121901.
HOGENOMiHOG000015135.
HOVERGENiHBG003564.
InParanoidiQ8K1Y2.
KOiK06070.
OrthoDBiEOG75B84N.
PhylomeDBiQ8K1Y2.

Miscellaneous databases

ChiTaRSiPrkd3. mouse.
NextBioi342658.
PROiQ8K1Y2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K1Y2.
CleanExiMM_PRKD3.
ExpressionAtlasiQ8K1Y2. baseline and differential.
GenevestigatoriQ8K1Y2.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR015727. Protein_Kinase_C_mu-related.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR22968. PTHR22968. 1 hit.
PfamiPF00130. C1_1. 2 hits.
PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000552. PKC_mu_nu_D2. 1 hit.
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00233. PH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213 AND SER-216, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiKPCD3_MOUSE
AccessioniPrimary (citable) accession number: Q8K1Y2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 1, 2002
Last modified: May 27, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.