Serine/threonine-protein kinase D3 - Mus musculus (Mouse)

Contribute

Send feedback

Read comments (?) or add your own

Q8K1Y2 (KPCD3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase D3
EC=2.7.11.13

Alternative name(s):
Protein kinase C nu type
nPKC-nu

Gene names

Name:	Prkd3
Synonyms:	Prkcn

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	889 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains 1 and 2 bind both DAG and phorbol ester with high affinity and mediate translocation to the cell membrane. Autophosphorylation of Ser-734 and phosphorylation of Ser-730 by PKC relieves auto-inhibition by the PH domain.
Subcellular location	Cytoplasm By similarity. Membrane By similarity. Note: Translocation to the cell membrane is required for kinase activation By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Domain	Repeat Zinc-finger
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid binding Inferred from electronic annotation. Source: InterPro protein kinase C activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 889

889

Serine/threonine-protein kinase D3

PRO_0000055718

Regions

Domain

416 – 532

117

Domain

575 – 831

257

Protein kinase

Zinc finger

154 – 204

Phorbol-ester/DAG-type 1

Zinc finger

271 – 321

Phorbol-ester/DAG-type 2

Nucleotide binding

581 – 589

ATP By similarity

Sites

Active site

698

Proton acceptor By similarity

Binding site

604

ATP By similarity

Amino acid modifications

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine Ref.3

Modified residue

Phosphoserine Ref.3

Modified residue

213

Phosphoserine Ref.3

Modified residue

216

Phosphoserine Ref.3

Modified residue

364

Phosphoserine Ref.2

Modified residue

535

Phosphothreonine By similarity

Modified residue

730

Phosphoserine; by PKC By similarity

Modified residue

734

Phosphoserine; by autocatalysis By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q8K1Y2 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 7D03938ED4ECC2E4
Show »

FASTA

889

100,078

        10         20         30         40         50         60 
MSANNSPPSA QKSVFPATVS AVLPAPSPCS SPKTGLSARL SNGSFSAPSL TNSRGSVHTV 

        70         80         90        100        110        120 
SFLLQIGLTR ESVTIEAQEL SLSAVKDLVC SIVYQKFPEC GFFGMYDKIL LFRHDMNSEN 

       130        140        150        160        170        180 
ILQLITSADE IHEGDLVEVV LSALATVEDF QIRPHALYVH SYKAPTFCDY CGEMLWGLVR 

       190        200        210        220        230        240 
QGLKCEGCGL NYHKRCAFKI PNNCSGVRKR RLSNVSLPGP GLSVPRPLQP ECVPLLSEES 

       250        260        270        280        290        300 
HTHQEPSKRI PSWSGRPIWM EKMVMCRVKV PHTFAVHSYG RPTICQYCKR LLKGLFRQGM 

       310        320        330        340        350        360 
QCKDCKFNCH KRCASKVPRD CLGEVTFNGE PCSVGTDADM PMDIDSSDVN SDGSRGLDDS 

       370        380        390        400        410        420 
EEPSPPEDKM FFLDPTDLDV ERDEETVKTI SPSTSNNIPL MRVVQSIKHT KRRSSTVVKE 

       430        440        450        460        470        480 
GWMVHYTSRD NLRKRHYWRL DSKCLTLFQN ESGSKYYKEI PLSEILRVSS PQDFTSISQG 

       490        500        510        520        530        540 
SNPHCFEIIT DTVVYFVGEN NGSSSHNPVL AATGVGLDVA QSWEKAIRQA LMPVTPQASV 

       550        560        570        580        590        600 
CTSPGQGKDH NLATSISVSN CQVQENVDIS SVYQIFADEV LGSGQFGIVY GGKHRKTGRD 

       610        620        630        640        650        660 
VAIKVIDKMR FPTKQESQLR NEVAILQNLH HPGIVNLECM FETPERVFVV MEKLHGDMLE 

       670        680        690        700        710        720 
MILSSEKSRL PERITKFMVT QILVALRNLH FKNIVHCDLK PENVLLASAE PFPQVKLCDF 

       730        740        750        760        770        780 
GFARIIGEKS FRRSVVGTPA YLAPEVLRSK GYNRSLDMWS VGVIVYVSLS GTFPFNEDED 

       790        800        810        820        830        840 
INDQIQNAAF MYPPNPWREI SSEAIDLINN LLQVKMRKRY SVDKSLSHPW LQDYQTWLDL 

       850        860        870        880 
REFETRIGER YITHESDDAR WEIHAYTHNL EYPKHFIMAP NPDDMEEDP

« Hide

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[2]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-364, MASS SPECTROMETRY. Tissue: Liver.
[3]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41; SER-44; SER-213 AND SER-216, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC037012 mRNA. Translation: AAH37012.1.
IPI	IPI00322006.
RefSeq	NP_083515.2. NM_029239.3.
UniGene	Mm.252776.
3D structure databases
ProteinModelPortal	Q8K1Y2.
SMR	Q8K1Y2. Positions 155-204, 272-321, 414-532, 539-884.
ModBase	Search...
PTM databases
PhosphoSite	Q8K1Y2.
Proteomic databases
PaxDb	Q8K1Y2.
PRIDE	Q8K1Y2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000003191; ENSMUSP00000003191; ENSMUSG00000024070. ENSMUST00000168887; ENSMUSP00000132004; ENSMUSG00000024070.
GeneID	75292.
KEGG	mmu:75292.
UCSC	uc008dpr.2. mouse.
Organism-specific databases
CTD	23683.
MGI	MGI:1922542. Prkd3.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00680000099894.
HOGENOM	HOG000015135.
HOVERGEN	HBG003564.
KO	K06070.
OrthoDB	EOG4548XW.
Gene expression databases
ArrayExpress	Q8K1Y2.
Bgee	Q8K1Y2.
CleanEx	MM_PRKD3.
Genevestigator	Q8K1Y2.
GermOnline	ENSMUSG00000024070. Mus musculus.
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR020454. DAG/PE-bd. IPR011009. Kinase-like_dom. IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR015727. Protein_Kinase_C_mu-related. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
PANTHER	PTHR22968. PTHR22968. 1 hit.
Pfam	PF00130. C1_1. 2 hits. PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF000552. PKC_mu_nu_D2. 1 hit.
PRINTS	PR00008. DAGPEDOMAIN.
SMART	SM00109. C1. 2 hits. SM00233. PH. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	342658.
SOURCE	Search...

Entry information

Entry name

KPCD3_MOUSE

Accession

Primary (citable) accession number: Q8K1Y2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents