Reviewed,
UniProtKB/Swiss-Prot Q8K1Y2 (KPCD3_MOUSE)
Last modified
February 9, 2010.
Version 69.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Serine/threonine-protein kinase D3 EC=2.7.11.13 Alternative name(s): Protein kinase C nu type nPKC-nu | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 889 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress By similarity. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Cofactor | Magnesium By similarity. |
| Enzyme regulation | Activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains 1 and 2 bind both DAG and phorbol ester with high affinity and mediate translocation to the cell membrane. Autophosphorylation of Ser-734 and phosphorylation of Ser-730 by PKC relieves auto-inhibition by the PH domain. |
| Subcellular location | Cytoplasm By similarity. Membrane By similarity. Note: Translocation to the cell membrane is required for kinase activation By similarity. |
| Sequence similarities | Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Membrane |
| Domain | Repeat Zinc-finger |
| Ligand | ATP-binding Magnesium Metal-binding Nucleotide-binding Zinc |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | intracellular signaling cascade Inferred from electronic annotation. Source: InterPro protein amino acid phosphorylationInferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW protein kinase C activityInferred from electronic annotation. Source: EC zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 889 | 889 | Serine/threonine-protein kinase D3 | PRO_0000055718 | |||||
Regions | |||||||||
| Domain | 416 – 532 | 117 | PH | ||||||
| Domain | 575 – 831 | 257 | Protein kinase | ||||||
| Zinc finger | 154 – 204 | 51 | Phorbol-ester/DAG-type 1 | ||||||
| Zinc finger | 271 – 321 | 51 | Phorbol-ester/DAG-type 2 | ||||||
| Nucleotide binding | 581 – 589 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 698 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 604 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 6 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 27 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 30 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 31 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 34 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 37 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 41 | 1 | Phosphoserine Ref.3 | ||||||
| Modified residue | 44 | 1 | Phosphoserine Ref.3 | ||||||
| Modified residue | 213 | 1 | Phosphoserine Ref.3 | ||||||
| Modified residue | 216 | 1 | Phosphoserine Ref.3 | ||||||
| Modified residue | 237 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 247 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 252 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 364 | 1 | Phosphoserine Ref.2 | ||||||
| Modified residue | 391 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 395 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 414 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 415 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 535 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 539 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 542 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 543 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 730 | 1 | Phosphoserine; by PKC By similarity | ||||||
| Modified residue | 734 | 1 | Phosphoserine; by autocatalysis By similarity | ||||||
Sequences
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References
| [1] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor. |
| [2] | "Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-364, MASS SPECTROMETRY. Tissue: Liver. |
| [3] | "Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41; SER-44; SER-213 AND SER-216, MASS SPECTROMETRY. Tissue: Liver. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BC037012 mRNA. Translation: AAH37012.1. |
| IPI | IPI00322006. |
| RefSeq | NP_083515.2. |
| UniGene | Mm.252776 |
3D structure databases | |
| SMR | Q8K1Y2. Positions 147-210, 269-322, 414-532, 537-835. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q8K1Y2. |
PTM databases | |
| PhosphoSite | Q8K1Y2. |
Proteomic databases | |
| PRIDE | Q8K1Y2. |
Genome annotation databases | |
| Ensembl | ENSMUST00000003191; ENSMUSP00000003191; ENSMUSG00000024070; Mus musculus. [Genome view] |
| GeneID | 75292. |
| KEGG | mmu:75292. |
| UCSC | uc008dpr.1. mouse. |
Organism-specific databases | |
| CTD | 75292. |
| MGI | MGI:1922542. Prkd3. |
Phylogenomic databases | |
| HOVERGEN | Q8K1Y2. |
| OMA | NVTIEAQ. |
| PhylomeDB | Q8K1Y2. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.13. 244. |
Gene expression databases | |
| ArrayExpress | Q8K1Y2. |
| Bgee | Q8K1Y2. |
| CleanEx | MM_PRKD3. |
| Genevestigator | Q8K1Y2. |
| GermOnline | ENSMUSG00000024070. Mus musculus. |
Family and domain databases | |
| InterPro | IPR020454. DAG/PE_bd. IPR011009. Kinase-like_dom. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR015727. Protein_Kinase_C_mu-related. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view] |
| Gene3D | G3DSA:2.30.29.30. PH_type. 1 hit. |
| PANTHER | PTHR22968. PKC_mu_like. 1 hit. |
| Pfam | PF00130. C1_1. 2 hits. PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view] |
| PRINTS | PR00008. DAGPEDOMAIN. |
| SMART | SM00109. C1. 2 hits. SM00233. PH. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 342658. |
| SOURCE | Search... |
Entry information
| Entry name | KPCD3_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q8K1Y2 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

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