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Q8K1Y2

- KPCD3_MOUSE

UniProt

Q8K1Y2 - KPCD3_MOUSE

Protein

Serine/threonine-protein kinase D3

Gene

Prkd3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains 1 and 2 bind both DAG and phorbol ester with high affinity and mediate translocation to the cell membrane. Autophosphorylation of Ser-734 and phosphorylation of Ser-730 by PKC relieves auto-inhibition by the PH domain.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei604 – 6041ATPPROSITE-ProRule annotation
    Active sitei698 – 6981Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri154 – 20451Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri271 – 32151Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi581 – 5899ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein kinase C activity Source: UniProtKB-EC

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase D3 (EC:2.7.11.13)
    Alternative name(s):
    Protein kinase C nu type
    nPKC-nu
    Gene namesi
    Name:Prkd3
    Synonyms:Prkcn
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1922542. Prkd3.

    Subcellular locationi

    Cytoplasm By similarity. Membrane By similarity
    Note: Translocation to the cell membrane is required for kinase activation.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 889889Serine/threonine-protein kinase D3PRO_0000055718Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei6 – 61PhosphoserineBy similarity
    Modified residuei27 – 271PhosphoserineBy similarity
    Modified residuei37 – 371PhosphoserineBy similarity
    Modified residuei41 – 411PhosphoserineBy similarity
    Modified residuei44 – 441PhosphoserineBy similarity
    Modified residuei213 – 2131Phosphoserine1 Publication
    Modified residuei216 – 2161Phosphoserine1 Publication
    Modified residuei364 – 3641PhosphoserineBy similarity
    Modified residuei535 – 5351PhosphothreonineBy similarity
    Modified residuei730 – 7301Phosphoserine; by PKCBy similarity
    Modified residuei734 – 7341Phosphoserine; by autocatalysisBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8K1Y2.
    PRIDEiQ8K1Y2.

    PTM databases

    PhosphoSiteiQ8K1Y2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8K1Y2.
    BgeeiQ8K1Y2.
    CleanExiMM_PRKD3.
    GenevestigatoriQ8K1Y2.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K1Y2.
    SMRiQ8K1Y2. Positions 155-204, 272-321, 414-532, 539-864.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini416 – 532117PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini575 – 831257Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri154 – 20451Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri271 – 32151Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117523.
    HOGENOMiHOG000015135.
    HOVERGENiHBG003564.
    KOiK06070.
    OrthoDBiEOG75B84N.
    PhylomeDBiQ8K1Y2.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    InterProiIPR020454. DAG/PE-bd.
    IPR011009. Kinase-like_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR015727. Protein_Kinase_C_mu-related.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR22968. PTHR22968. 1 hit.
    PfamiPF00130. C1_1. 2 hits.
    PF00169. PH. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000552. PKC_mu_nu_D2. 1 hit.
    PRINTSiPR00008. DAGPEDOMAIN.
    SMARTiSM00109. C1. 2 hits.
    SM00233. PH. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50003. PH_DOMAIN. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8K1Y2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSANNSPPSA QKSVFPATVS AVLPAPSPCS SPKTGLSARL SNGSFSAPSL    50
    TNSRGSVHTV SFLLQIGLTR ESVTIEAQEL SLSAVKDLVC SIVYQKFPEC 100
    GFFGMYDKIL LFRHDMNSEN ILQLITSADE IHEGDLVEVV LSALATVEDF 150
    QIRPHALYVH SYKAPTFCDY CGEMLWGLVR QGLKCEGCGL NYHKRCAFKI 200
    PNNCSGVRKR RLSNVSLPGP GLSVPRPLQP ECVPLLSEES HTHQEPSKRI 250
    PSWSGRPIWM EKMVMCRVKV PHTFAVHSYG RPTICQYCKR LLKGLFRQGM 300
    QCKDCKFNCH KRCASKVPRD CLGEVTFNGE PCSVGTDADM PMDIDSSDVN 350
    SDGSRGLDDS EEPSPPEDKM FFLDPTDLDV ERDEETVKTI SPSTSNNIPL 400
    MRVVQSIKHT KRRSSTVVKE GWMVHYTSRD NLRKRHYWRL DSKCLTLFQN 450
    ESGSKYYKEI PLSEILRVSS PQDFTSISQG SNPHCFEIIT DTVVYFVGEN 500
    NGSSSHNPVL AATGVGLDVA QSWEKAIRQA LMPVTPQASV CTSPGQGKDH 550
    NLATSISVSN CQVQENVDIS SVYQIFADEV LGSGQFGIVY GGKHRKTGRD 600
    VAIKVIDKMR FPTKQESQLR NEVAILQNLH HPGIVNLECM FETPERVFVV 650
    MEKLHGDMLE MILSSEKSRL PERITKFMVT QILVALRNLH FKNIVHCDLK 700
    PENVLLASAE PFPQVKLCDF GFARIIGEKS FRRSVVGTPA YLAPEVLRSK 750
    GYNRSLDMWS VGVIVYVSLS GTFPFNEDED INDQIQNAAF MYPPNPWREI 800
    SSEAIDLINN LLQVKMRKRY SVDKSLSHPW LQDYQTWLDL REFETRIGER 850
    YITHESDDAR WEIHAYTHNL EYPKHFIMAP NPDDMEEDP 889
    Length:889
    Mass (Da):100,078
    Last modified:October 1, 2002 - v1
    Checksum:i7D03938ED4ECC2E4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC037012 mRNA. Translation: AAH37012.1.
    CCDSiCCDS37699.1.
    RefSeqiNP_083515.2. NM_029239.3.
    UniGeneiMm.252776.

    Genome annotation databases

    EnsembliENSMUST00000003191; ENSMUSP00000003191; ENSMUSG00000024070.
    ENSMUST00000168887; ENSMUSP00000132004; ENSMUSG00000024070.
    GeneIDi75292.
    KEGGimmu:75292.
    UCSCiuc008dpr.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC037012 mRNA. Translation: AAH37012.1 .
    CCDSi CCDS37699.1.
    RefSeqi NP_083515.2. NM_029239.3.
    UniGenei Mm.252776.

    3D structure databases

    ProteinModelPortali Q8K1Y2.
    SMRi Q8K1Y2. Positions 155-204, 272-321, 414-532, 539-864.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8K1Y2.

    Proteomic databases

    PaxDbi Q8K1Y2.
    PRIDEi Q8K1Y2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000003191 ; ENSMUSP00000003191 ; ENSMUSG00000024070 .
    ENSMUST00000168887 ; ENSMUSP00000132004 ; ENSMUSG00000024070 .
    GeneIDi 75292.
    KEGGi mmu:75292.
    UCSCi uc008dpr.2. mouse.

    Organism-specific databases

    CTDi 23683.
    MGIi MGI:1922542. Prkd3.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117523.
    HOGENOMi HOG000015135.
    HOVERGENi HBG003564.
    KOi K06070.
    OrthoDBi EOG75B84N.
    PhylomeDBi Q8K1Y2.

    Miscellaneous databases

    NextBioi 342658.
    PROi Q8K1Y2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8K1Y2.
    Bgeei Q8K1Y2.
    CleanExi MM_PRKD3.
    Genevestigatori Q8K1Y2.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    InterProi IPR020454. DAG/PE-bd.
    IPR011009. Kinase-like_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR015727. Protein_Kinase_C_mu-related.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR22968. PTHR22968. 1 hit.
    Pfami PF00130. C1_1. 2 hits.
    PF00169. PH. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000552. PKC_mu_nu_D2. 1 hit.
    PRINTSi PR00008. DAGPEDOMAIN.
    SMARTi SM00109. C1. 2 hits.
    SM00233. PH. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50003. PH_DOMAIN. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213 AND SER-216, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiKPCD3_MOUSE
    AccessioniPrimary (citable) accession number: Q8K1Y2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3