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Protein

Transmembrane protease serine 3

Gene

Tmprss3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable serine protease that plays a role in hearing. Acts as a permissive factor for cochlear hair cell survival and activation at the onset of hearing and is required for saccular hair cell survival. Activates ENaC (in vitro).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei257Charge relay systemBy similarity1
Active sitei304Charge relay systemBy similarity1
Active sitei400Charge relay systemCurated1

GO - Molecular functioni

GO - Biological processi

  • cellular sodium ion homeostasis Source: MGI
  • sensory perception of sound Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.079.

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane protease serine 3 (EC:3.4.21.-)
Gene namesi
Name:Tmprss3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2155445. Tmprss3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 48CytoplasmicSequence analysisAdd BLAST48
Transmembranei49 – 69Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini70 – 453ExtracellularSequence analysisAdd BLAST384

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • neuronal cell body Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000886911 – 453Transmembrane protease serine 3Add BLAST453

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi73 ↔ 85By similarity
Disulfide bondi79 ↔ 98By similarity
Disulfide bondi92 ↔ 107By similarity
Disulfide bondi129 ↔ 194By similarity
Disulfide bondi142 ↔ 204By similarity
Disulfide bondi207 ↔ 324By similarity
Glycosylationi221N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi242 ↔ 258By similarity
Disulfide bondi338 ↔ 406By similarity
Disulfide bondi369 ↔ 385By similarity
Disulfide bondi396 ↔ 424By similarity

Post-translational modificationi

Undergoes autoproteolytic activation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei216 – 217CleavageSequence analysis2

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ8K1T0.
PRIDEiQ8K1T0.

PTM databases

PhosphoSitePlusiQ8K1T0.

Expressioni

Tissue specificityi

Strongly expressed in liver, cochlea, brain, cerebellum, spleen, lung, and muscle and at a lower degree in retina, kidney, and heart. Expressed in the spiral ganglion, the cells supporting the organ of Corti and the stria vascularis. Isoform 2 is strongly expressed only in the cochlea with very faint expression in the cerebellum, spleen and muscle.1 Publication

Gene expression databases

BgeeiENSMUSG00000024034.
CleanExiMM_TMPRSS3.
ExpressionAtlasiQ8K1T0. baseline and differential.
GenevisibleiQ8K1T0. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000110196.

Structurei

3D structure databases

ProteinModelPortaliQ8K1T0.
SMRiQ8K1T0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 108LDL-receptor class APROSITE-ProRule annotationAdd BLAST37
Domaini104 – 205SRCRPROSITE-ProRule annotationAdd BLAST102
Domaini217 – 448Peptidase S1PROSITE-ProRule annotationAdd BLAST232

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 LDL-receptor class A domain.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
Contains 1 SRCR domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118962.
HOGENOMiHOG000251822.
HOVERGENiHBG013304.
InParanoidiQ8K1T0.
KOiK09634.
OMAiVITPLWI.
TreeFamiTF351678.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
Gene3Di3.10.250.10. 1 hit.
4.10.400.10. 1 hit.
InterProiIPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00057. Ldl_recept_a. 1 hit.
PF15494. SRCR_2. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00192. LDLa. 1 hit.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 1 hit.
PROSITEiPS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K1T0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGENDPPAAE APFSFRSLFG LDDLKISPVA PDGDAVAAQI LSLLPLKFFP
60 70 80 90 100
IIVIGIIALI LALAIGLGIH FDCSGKYRCH SSFKCIELTA RCDGVSDCKN
110 120 130 140 150
AEDEYRCVRV SGQRAALQVF TAAAWRTMCS DDWKSHYAKI ACAQLGFPSY
160 170 180 190 200
VSSDHLRVDA LEEQFQGDFV SINHLLSDDK VTALHHSVYM REGCTSGHVV
210 220 230 240 250
TLKCSACGTR TGYSPRIVGG NMSSLTQWPW QVSLQFQGYH LCGGSIITPL
260 270 280 290 300
WIVTAAHCVY DLYHPKSWTV QVGLVSLMDS PVPSHLVEKI IYHSKYKPKR
310 320 330 340 350
LGNDIALMKL SEPLTFDETI QPICLPNSEE NFPDGKLCWT SGWGATEDGG
360 370 380 390 400
DASPVLNHAA VPLISNKICN HRDVYGGIIS PSMLCAGYLK GGVDSCQGDS
410 420 430 440 450
GGPLVCQERR LWKLVGATSF GIGCAEVNKP GVYTRITSFL DWIHEQLERD

LKT
Length:453
Mass (Da):49,506
Last modified:July 27, 2011 - v2
Checksum:i1EE7ECD6CB3DD894
GO
Isoform 2 (identifier: Q8K1T0-2) [UniParc]FASTAAdd to basket
Also known as: F

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAASEMVEVEPEPNIRGPEIVTM

Show »
Length:475
Mass (Da):51,886
Checksum:i0214D1E550EC917B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti117L → H in CAC83350 (PubMed:12393794).Curated1
Sequence conflicti246I → V in CAD22137 (PubMed:12393794).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0415811M → MAASEMVEVEPEPNIRGPEI VTM in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ429216 Genomic DNA. Translation: CAD22137.1.
AJ300738 mRNA. Translation: CAC83350.1.
AY261383 Genomic DNA. Translation: AAO33581.1.
CCDSiCCDS37547.1. [Q8K1T0-1]
CCDS50053.1. [Q8K1T0-2]
RefSeqiNP_542765.2. NM_080727.2. [Q8K1T0-1]
UniGeneiMm.214638.

Genome annotation databases

EnsembliENSMUST00000024833; ENSMUSP00000024833; ENSMUSG00000024034. [Q8K1T0-1]
ENSMUST00000114549; ENSMUSP00000110196; ENSMUSG00000024034. [Q8K1T0-2]
GeneIDi140765.
KEGGimmu:140765.
UCSCiuc008bup.2. mouse. [Q8K1T0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ429216 Genomic DNA. Translation: CAD22137.1.
AJ300738 mRNA. Translation: CAC83350.1.
AY261383 Genomic DNA. Translation: AAO33581.1.
CCDSiCCDS37547.1. [Q8K1T0-1]
CCDS50053.1. [Q8K1T0-2]
RefSeqiNP_542765.2. NM_080727.2. [Q8K1T0-1]
UniGeneiMm.214638.

3D structure databases

ProteinModelPortaliQ8K1T0.
SMRiQ8K1T0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000110196.

Protein family/group databases

MEROPSiS01.079.

PTM databases

PhosphoSitePlusiQ8K1T0.

Proteomic databases

PaxDbiQ8K1T0.
PRIDEiQ8K1T0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024833; ENSMUSP00000024833; ENSMUSG00000024034. [Q8K1T0-1]
ENSMUST00000114549; ENSMUSP00000110196; ENSMUSG00000024034. [Q8K1T0-2]
GeneIDi140765.
KEGGimmu:140765.
UCSCiuc008bup.2. mouse. [Q8K1T0-1]

Organism-specific databases

CTDi64699.
MGIiMGI:2155445. Tmprss3.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118962.
HOGENOMiHOG000251822.
HOVERGENiHBG013304.
InParanoidiQ8K1T0.
KOiK09634.
OMAiVITPLWI.
TreeFamiTF351678.

Miscellaneous databases

PROiQ8K1T0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024034.
CleanExiMM_TMPRSS3.
ExpressionAtlasiQ8K1T0. baseline and differential.
GenevisibleiQ8K1T0. MM.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
Gene3Di3.10.250.10. 1 hit.
4.10.400.10. 1 hit.
InterProiIPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00057. Ldl_recept_a. 1 hit.
PF15494. SRCR_2. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00192. LDLa. 1 hit.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 1 hit.
PROSITEiPS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTMPS3_MOUSE
AccessioniPrimary (citable) accession number: Q8K1T0
Secondary accession number(s): Q812A6, Q8VDE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2003
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.