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Q8K1T0

- TMPS3_MOUSE

UniProt

Q8K1T0 - TMPS3_MOUSE

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Protein

Transmembrane protease serine 3

Gene
Tmprss3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Probable serine protease that play a role in hearing. Acts as a permissive factor for cochlear hair cells survival and activation at the onset of hearing and is required for saccular hair cell survival. Activates ENaC (in vitro).2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei216 – 2172Cleavage Reviewed prediction
Active sitei257 – 2571Charge relay system By similarity
Active sitei304 – 3041Charge relay system By similarity
Active sitei400 – 4001Charge relay system Inferred

GO - Molecular functioni

  1. scavenger receptor activity Source: InterPro
  2. serine-type endopeptidase activity Source: InterPro
  3. sodium channel regulator activity Source: MGI

GO - Biological processi

  1. cellular sodium ion homeostasis Source: MGI
  2. sensory perception of sound Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.079.

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane protease serine 3 (EC:3.4.21.-)
Gene namesi
Name:Tmprss3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:2155445. Tmprss3.

Subcellular locationi

Endoplasmic reticulum membrane; Single-pass type II membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4848Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei49 – 6921Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini70 – 453384Extracellular Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. endoplasmic reticulum membrane Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
  4. neuronal cell body Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 453453Transmembrane protease serine 3PRO_0000088691Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi73 ↔ 85 By similarity
Disulfide bondi79 ↔ 98 By similarity
Disulfide bondi92 ↔ 107 By similarity
Disulfide bondi129 ↔ 194 By similarity
Disulfide bondi142 ↔ 204 By similarity
Disulfide bondi207 ↔ 324 By similarity
Glycosylationi221 – 2211N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi242 ↔ 258 By similarity
Disulfide bondi338 ↔ 406 By similarity
Disulfide bondi369 ↔ 385 By similarity
Disulfide bondi396 ↔ 424 By similarity

Post-translational modificationi

Undergoes autoproteolytic activation.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiQ8K1T0.

PTM databases

PhosphoSiteiQ8K1T0.

Expressioni

Tissue specificityi

Strongly expressed in liver, cochlea, brain, cerebellum, spleen, lung, and muscle and at a lower degree in retina, kidney, and heart. Expressed in the spiral ganglion, the cells supporting the organ of Corti and the stria vascularis. Isoform 2 is strongly expressed only in the cochlea with very faint expression in the cerebellum, spleen and muscle.1 Publication

Gene expression databases

BgeeiQ8K1T0.
CleanExiMM_TMPRSS3.
GenevestigatoriQ8K1T0.

Structurei

3D structure databases

ProteinModelPortaliQ8K1T0.
SMRiQ8K1T0. Positions 108-448.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 10837LDL-receptor class AAdd
BLAST
Domaini104 – 205102SRCRAdd
BLAST
Domaini217 – 448232Peptidase S1Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.
Contains 1 SRCR domain.

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00730000110467.
HOGENOMiHOG000251822.
HOVERGENiHBG013304.
InParanoidiQ812A6.
KOiK09634.
OMAiVITPLWI.
OrthoDBiEOG75B84T.
TreeFamiTF351678.

Family and domain databases

Gene3Di3.10.250.10. 1 hit.
4.10.400.10. 1 hit.
InterProiIPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00057. Ldl_recept_a. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00192. LDLa. 1 hit.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 1 hit.
PROSITEiPS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8K1T0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGENDPPAAE APFSFRSLFG LDDLKISPVA PDGDAVAAQI LSLLPLKFFP    50
IIVIGIIALI LALAIGLGIH FDCSGKYRCH SSFKCIELTA RCDGVSDCKN 100
AEDEYRCVRV SGQRAALQVF TAAAWRTMCS DDWKSHYAKI ACAQLGFPSY 150
VSSDHLRVDA LEEQFQGDFV SINHLLSDDK VTALHHSVYM REGCTSGHVV 200
TLKCSACGTR TGYSPRIVGG NMSSLTQWPW QVSLQFQGYH LCGGSIITPL 250
WIVTAAHCVY DLYHPKSWTV QVGLVSLMDS PVPSHLVEKI IYHSKYKPKR 300
LGNDIALMKL SEPLTFDETI QPICLPNSEE NFPDGKLCWT SGWGATEDGG 350
DASPVLNHAA VPLISNKICN HRDVYGGIIS PSMLCAGYLK GGVDSCQGDS 400
GGPLVCQERR LWKLVGATSF GIGCAEVNKP GVYTRITSFL DWIHEQLERD 450
LKT 453
Length:453
Mass (Da):49,506
Last modified:July 27, 2011 - v2
Checksum:i1EE7ECD6CB3DD894
GO
Isoform 2 (identifier: Q8K1T0-2) [UniParc]FASTAAdd to Basket

Also known as: F

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAASEMVEVEPEPNIRGPEIVTM

Show »
Length:475
Mass (Da):51,886
Checksum:i0214D1E550EC917B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MAASEMVEVEPEPNIRGPEI VTM in isoform 2. VSP_041581

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti117 – 1171L → H in CAC83350. 1 Publication
Sequence conflicti246 – 2461I → V in CAD22137. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ429216 Genomic DNA. Translation: CAD22137.1.
AJ300738 mRNA. Translation: CAC83350.1.
AY261383 Genomic DNA. Translation: AAO33581.1.
CCDSiCCDS37547.1. [Q8K1T0-1]
CCDS50053.1. [Q8K1T0-2]
RefSeqiNP_542765.2. NM_080727.2. [Q8K1T0-1]
UniGeneiMm.214638.

Genome annotation databases

EnsembliENSMUST00000024833; ENSMUSP00000024833; ENSMUSG00000024034. [Q8K1T0-1]
ENSMUST00000114549; ENSMUSP00000110196; ENSMUSG00000024034. [Q8K1T0-2]
GeneIDi140765.
KEGGimmu:140765.
UCSCiuc008bup.2. mouse. [Q8K1T0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ429216 Genomic DNA. Translation: CAD22137.1 .
AJ300738 mRNA. Translation: CAC83350.1 .
AY261383 Genomic DNA. Translation: AAO33581.1 .
CCDSi CCDS37547.1. [Q8K1T0-1 ]
CCDS50053.1. [Q8K1T0-2 ]
RefSeqi NP_542765.2. NM_080727.2. [Q8K1T0-1 ]
UniGenei Mm.214638.

3D structure databases

ProteinModelPortali Q8K1T0.
SMRi Q8K1T0. Positions 108-448.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S01.079.

PTM databases

PhosphoSitei Q8K1T0.

Proteomic databases

PRIDEi Q8K1T0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000024833 ; ENSMUSP00000024833 ; ENSMUSG00000024034 . [Q8K1T0-1 ]
ENSMUST00000114549 ; ENSMUSP00000110196 ; ENSMUSG00000024034 . [Q8K1T0-2 ]
GeneIDi 140765.
KEGGi mmu:140765.
UCSCi uc008bup.2. mouse. [Q8K1T0-1 ]

Organism-specific databases

CTDi 64699.
MGIi MGI:2155445. Tmprss3.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00730000110467.
HOGENOMi HOG000251822.
HOVERGENi HBG013304.
InParanoidi Q812A6.
KOi K09634.
OMAi VITPLWI.
OrthoDBi EOG75B84T.
TreeFami TF351678.

Miscellaneous databases

NextBioi 369973.
PROi Q8K1T0.
SOURCEi Search...

Gene expression databases

Bgeei Q8K1T0.
CleanExi MM_TMPRSS3.
Genevestigatori Q8K1T0.

Family and domain databases

Gene3Di 3.10.250.10. 1 hit.
4.10.400.10. 1 hit.
InterProi IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00057. Ldl_recept_a. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00192. LDLa. 1 hit.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 1 hit.
PROSITEi PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SUBCELLULAR LOCATION, FUNCTION IN ENAC CLEAVAGE.
  2. "Genomic organization of murine transmembrane proteinases."
    Rao N.V., Rao G.N., Hoidal J.R.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Tmprss3, a transmembrane serine protease deficient in human DFNB8/10 deafness, is critical for cochlear hair cell survival at the onset of hearing."
    Fasquelle L., Scott H.S., Lenoir M., Wang J., Rebillard G., Gaboyard S., Venteo S., Francois F., Mausset-Bonnefont A.L., Antonarakis S.E., Neidhart E., Chabbert C., Puel J.L., Guipponi M., Delprat B.
    J. Biol. Chem. 286:17383-17397(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE SPLICING (ISOFORM 2).

Entry informationi

Entry nameiTMPS3_MOUSE
AccessioniPrimary (citable) accession number: Q8K1T0
Secondary accession number(s): Q812A6, Q8VDE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2003
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi