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Q8K1T0

- TMPS3_MOUSE

UniProt

Q8K1T0 - TMPS3_MOUSE

Protein

Transmembrane protease serine 3

Gene

Tmprss3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Probable serine protease that play a role in hearing. Acts as a permissive factor for cochlear hair cells survival and activation at the onset of hearing and is required for saccular hair cell survival. Activates ENaC (in vitro).2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei216 – 2172CleavageSequence Analysis
    Active sitei257 – 2571Charge relay systemBy similarity
    Active sitei304 – 3041Charge relay systemBy similarity
    Active sitei400 – 4001Charge relay systemCurated

    GO - Molecular functioni

    1. scavenger receptor activity Source: InterPro
    2. serine-type endopeptidase activity Source: InterPro
    3. sodium channel regulator activity Source: MGI

    GO - Biological processi

    1. cellular sodium ion homeostasis Source: MGI
    2. sensory perception of sound Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.079.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transmembrane protease serine 3 (EC:3.4.21.-)
    Gene namesi
    Name:Tmprss3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:2155445. Tmprss3.

    Subcellular locationi

    Endoplasmic reticulum membrane 2 Publications; Single-pass type II membrane protein 2 Publications

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. endoplasmic reticulum membrane Source: MGI
    3. integral component of membrane Source: UniProtKB-KW
    4. neuronal cell body Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 453453Transmembrane protease serine 3PRO_0000088691Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi73 ↔ 85By similarity
    Disulfide bondi79 ↔ 98By similarity
    Disulfide bondi92 ↔ 107By similarity
    Disulfide bondi129 ↔ 194By similarity
    Disulfide bondi142 ↔ 204By similarity
    Disulfide bondi207 ↔ 324By similarity
    Glycosylationi221 – 2211N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi242 ↔ 258By similarity
    Disulfide bondi338 ↔ 406By similarity
    Disulfide bondi369 ↔ 385By similarity
    Disulfide bondi396 ↔ 424By similarity

    Post-translational modificationi

    Undergoes autoproteolytic activation.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PRIDEiQ8K1T0.

    PTM databases

    PhosphoSiteiQ8K1T0.

    Expressioni

    Tissue specificityi

    Strongly expressed in liver, cochlea, brain, cerebellum, spleen, lung, and muscle and at a lower degree in retina, kidney, and heart. Expressed in the spiral ganglion, the cells supporting the organ of Corti and the stria vascularis. Isoform 2 is strongly expressed only in the cochlea with very faint expression in the cerebellum, spleen and muscle.1 Publication

    Gene expression databases

    BgeeiQ8K1T0.
    CleanExiMM_TMPRSS3.
    GenevestigatoriQ8K1T0.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K1T0.
    SMRiQ8K1T0. Positions 108-448.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4848CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini70 – 453384ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei49 – 6921Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini72 – 10837LDL-receptor class APROSITE-ProRule annotationAdd
    BLAST
    Domaini104 – 205102SRCRPROSITE-ProRule annotationAdd
    BLAST
    Domaini217 – 448232Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 LDL-receptor class A domain.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
    Contains 1 SRCR domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00730000110467.
    HOGENOMiHOG000251822.
    HOVERGENiHBG013304.
    InParanoidiQ812A6.
    KOiK09634.
    OMAiVITPLWI.
    OrthoDBiEOG75B84T.
    TreeFamiTF351678.

    Family and domain databases

    Gene3Di3.10.250.10. 1 hit.
    4.10.400.10. 1 hit.
    InterProiIPR023415. LDLR_class-A_CS.
    IPR002172. LDrepeatLR_classA_rpt.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR001190. SRCR.
    IPR017448. SRCR-like_dom.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00057. Ldl_recept_a. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00192. LDLa. 1 hit.
    SM00202. SR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF56487. SSF56487. 1 hit.
    SSF57424. SSF57424. 1 hit.
    PROSITEiPS01209. LDLRA_1. 1 hit.
    PS50068. LDLRA_2. 1 hit.
    PS50287. SRCR_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8K1T0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGENDPPAAE APFSFRSLFG LDDLKISPVA PDGDAVAAQI LSLLPLKFFP    50
    IIVIGIIALI LALAIGLGIH FDCSGKYRCH SSFKCIELTA RCDGVSDCKN 100
    AEDEYRCVRV SGQRAALQVF TAAAWRTMCS DDWKSHYAKI ACAQLGFPSY 150
    VSSDHLRVDA LEEQFQGDFV SINHLLSDDK VTALHHSVYM REGCTSGHVV 200
    TLKCSACGTR TGYSPRIVGG NMSSLTQWPW QVSLQFQGYH LCGGSIITPL 250
    WIVTAAHCVY DLYHPKSWTV QVGLVSLMDS PVPSHLVEKI IYHSKYKPKR 300
    LGNDIALMKL SEPLTFDETI QPICLPNSEE NFPDGKLCWT SGWGATEDGG 350
    DASPVLNHAA VPLISNKICN HRDVYGGIIS PSMLCAGYLK GGVDSCQGDS 400
    GGPLVCQERR LWKLVGATSF GIGCAEVNKP GVYTRITSFL DWIHEQLERD 450
    LKT 453
    Length:453
    Mass (Da):49,506
    Last modified:July 27, 2011 - v2
    Checksum:i1EE7ECD6CB3DD894
    GO
    Isoform 2 (identifier: Q8K1T0-2) [UniParc]FASTAAdd to Basket

    Also known as: F

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MAASEMVEVEPEPNIRGPEIVTM

    Show »
    Length:475
    Mass (Da):51,886
    Checksum:i0214D1E550EC917B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti117 – 1171L → H in CAC83350. (PubMed:12393794)Curated
    Sequence conflicti246 – 2461I → V in CAD22137. (PubMed:12393794)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → MAASEMVEVEPEPNIRGPEI VTM in isoform 2. CuratedVSP_041581

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ429216 Genomic DNA. Translation: CAD22137.1.
    AJ300738 mRNA. Translation: CAC83350.1.
    AY261383 Genomic DNA. Translation: AAO33581.1.
    CCDSiCCDS37547.1. [Q8K1T0-1]
    CCDS50053.1. [Q8K1T0-2]
    RefSeqiNP_542765.2. NM_080727.2. [Q8K1T0-1]
    UniGeneiMm.214638.

    Genome annotation databases

    EnsembliENSMUST00000024833; ENSMUSP00000024833; ENSMUSG00000024034. [Q8K1T0-1]
    ENSMUST00000114549; ENSMUSP00000110196; ENSMUSG00000024034. [Q8K1T0-2]
    GeneIDi140765.
    KEGGimmu:140765.
    UCSCiuc008bup.2. mouse. [Q8K1T0-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ429216 Genomic DNA. Translation: CAD22137.1 .
    AJ300738 mRNA. Translation: CAC83350.1 .
    AY261383 Genomic DNA. Translation: AAO33581.1 .
    CCDSi CCDS37547.1. [Q8K1T0-1 ]
    CCDS50053.1. [Q8K1T0-2 ]
    RefSeqi NP_542765.2. NM_080727.2. [Q8K1T0-1 ]
    UniGenei Mm.214638.

    3D structure databases

    ProteinModelPortali Q8K1T0.
    SMRi Q8K1T0. Positions 108-448.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.079.

    PTM databases

    PhosphoSitei Q8K1T0.

    Proteomic databases

    PRIDEi Q8K1T0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000024833 ; ENSMUSP00000024833 ; ENSMUSG00000024034 . [Q8K1T0-1 ]
    ENSMUST00000114549 ; ENSMUSP00000110196 ; ENSMUSG00000024034 . [Q8K1T0-2 ]
    GeneIDi 140765.
    KEGGi mmu:140765.
    UCSCi uc008bup.2. mouse. [Q8K1T0-1 ]

    Organism-specific databases

    CTDi 64699.
    MGIi MGI:2155445. Tmprss3.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00730000110467.
    HOGENOMi HOG000251822.
    HOVERGENi HBG013304.
    InParanoidi Q812A6.
    KOi K09634.
    OMAi VITPLWI.
    OrthoDBi EOG75B84T.
    TreeFami TF351678.

    Miscellaneous databases

    NextBioi 369973.
    PROi Q8K1T0.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8K1T0.
    CleanExi MM_TMPRSS3.
    Genevestigatori Q8K1T0.

    Family and domain databases

    Gene3Di 3.10.250.10. 1 hit.
    4.10.400.10. 1 hit.
    InterProi IPR023415. LDLR_class-A_CS.
    IPR002172. LDrepeatLR_classA_rpt.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR001190. SRCR.
    IPR017448. SRCR-like_dom.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00057. Ldl_recept_a. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00192. LDLa. 1 hit.
    SM00202. SR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF56487. SSF56487. 1 hit.
    SSF57424. SSF57424. 1 hit.
    PROSITEi PS01209. LDLRA_1. 1 hit.
    PS50068. LDLRA_2. 1 hit.
    PS50287. SRCR_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SUBCELLULAR LOCATION, FUNCTION IN ENAC CLEAVAGE.
    2. "Genomic organization of murine transmembrane proteinases."
      Rao N.V., Rao G.N., Hoidal J.R.
      Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Tmprss3, a transmembrane serine protease deficient in human DFNB8/10 deafness, is critical for cochlear hair cell survival at the onset of hearing."
      Fasquelle L., Scott H.S., Lenoir M., Wang J., Rebillard G., Gaboyard S., Venteo S., Francois F., Mausset-Bonnefont A.L., Antonarakis S.E., Neidhart E., Chabbert C., Puel J.L., Guipponi M., Delprat B.
      J. Biol. Chem. 286:17383-17397(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE SPLICING (ISOFORM 2).

    Entry informationi

    Entry nameiTMPS3_MOUSE
    AccessioniPrimary (citable) accession number: Q8K1T0
    Secondary accession number(s): Q812A6, Q8VDE0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2003
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3