Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transmembrane protease serine 3

Gene

Tmprss3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable serine protease that play a role in hearing. Acts as a permissive factor for cochlear hair cells survival and activation at the onset of hearing and is required for saccular hair cell survival. Activates ENaC (in vitro).2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei216 – 2172CleavageSequence Analysis
Active sitei257 – 2571Charge relay systemBy similarity
Active sitei304 – 3041Charge relay systemBy similarity
Active sitei400 – 4001Charge relay systemCurated

GO - Molecular functioni

  1. scavenger receptor activity Source: InterPro
  2. serine-type endopeptidase activity Source: InterPro
  3. sodium channel regulator activity Source: MGI

GO - Biological processi

  1. cellular sodium ion homeostasis Source: MGI
  2. sensory perception of sound Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.079.

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane protease serine 3 (EC:3.4.21.-)
Gene namesi
Name:Tmprss3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2155445. Tmprss3.

Subcellular locationi

  1. Endoplasmic reticulum membrane 2 Publications; Single-pass type II membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4848CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei49 – 6921Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini70 – 453384ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. endoplasmic reticulum membrane Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
  4. neuronal cell body Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 453453Transmembrane protease serine 3PRO_0000088691Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi73 ↔ 85By similarity
Disulfide bondi79 ↔ 98By similarity
Disulfide bondi92 ↔ 107By similarity
Disulfide bondi129 ↔ 194By similarity
Disulfide bondi142 ↔ 204By similarity
Disulfide bondi207 ↔ 324By similarity
Glycosylationi221 – 2211N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi242 ↔ 258By similarity
Disulfide bondi338 ↔ 406By similarity
Disulfide bondi369 ↔ 385By similarity
Disulfide bondi396 ↔ 424By similarity

Post-translational modificationi

Undergoes autoproteolytic activation.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiQ8K1T0.

PTM databases

PhosphoSiteiQ8K1T0.

Expressioni

Tissue specificityi

Strongly expressed in liver, cochlea, brain, cerebellum, spleen, lung, and muscle and at a lower degree in retina, kidney, and heart. Expressed in the spiral ganglion, the cells supporting the organ of Corti and the stria vascularis. Isoform 2 is strongly expressed only in the cochlea with very faint expression in the cerebellum, spleen and muscle.1 Publication

Gene expression databases

BgeeiQ8K1T0.
CleanExiMM_TMPRSS3.
ExpressionAtlasiQ8K1T0. baseline and differential.
GenevestigatoriQ8K1T0.

Structurei

3D structure databases

ProteinModelPortaliQ8K1T0.
SMRiQ8K1T0. Positions 108-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 10837LDL-receptor class APROSITE-ProRule annotationAdd
BLAST
Domaini104 – 205102SRCRPROSITE-ProRule annotationAdd
BLAST
Domaini217 – 448232Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 LDL-receptor class A domain.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
Contains 1 SRCR domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118962.
HOGENOMiHOG000251822.
HOVERGENiHBG013304.
InParanoidiQ8K1T0.
KOiK09634.
OMAiVITPLWI.
OrthoDBiEOG75B84T.
TreeFamiTF351678.

Family and domain databases

Gene3Di3.10.250.10. 1 hit.
4.10.400.10. 1 hit.
InterProiIPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00057. Ldl_recept_a. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00192. LDLa. 1 hit.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 1 hit.
PROSITEiPS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K1T0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGENDPPAAE APFSFRSLFG LDDLKISPVA PDGDAVAAQI LSLLPLKFFP
60 70 80 90 100
IIVIGIIALI LALAIGLGIH FDCSGKYRCH SSFKCIELTA RCDGVSDCKN
110 120 130 140 150
AEDEYRCVRV SGQRAALQVF TAAAWRTMCS DDWKSHYAKI ACAQLGFPSY
160 170 180 190 200
VSSDHLRVDA LEEQFQGDFV SINHLLSDDK VTALHHSVYM REGCTSGHVV
210 220 230 240 250
TLKCSACGTR TGYSPRIVGG NMSSLTQWPW QVSLQFQGYH LCGGSIITPL
260 270 280 290 300
WIVTAAHCVY DLYHPKSWTV QVGLVSLMDS PVPSHLVEKI IYHSKYKPKR
310 320 330 340 350
LGNDIALMKL SEPLTFDETI QPICLPNSEE NFPDGKLCWT SGWGATEDGG
360 370 380 390 400
DASPVLNHAA VPLISNKICN HRDVYGGIIS PSMLCAGYLK GGVDSCQGDS
410 420 430 440 450
GGPLVCQERR LWKLVGATSF GIGCAEVNKP GVYTRITSFL DWIHEQLERD

LKT
Length:453
Mass (Da):49,506
Last modified:July 27, 2011 - v2
Checksum:i1EE7ECD6CB3DD894
GO
Isoform 2 (identifier: Q8K1T0-2) [UniParc]FASTAAdd to basket

Also known as: F

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAASEMVEVEPEPNIRGPEIVTM

Show »
Length:475
Mass (Da):51,886
Checksum:i0214D1E550EC917B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti117 – 1171L → H in CAC83350 (PubMed:12393794).Curated
Sequence conflicti246 – 2461I → V in CAD22137 (PubMed:12393794).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MAASEMVEVEPEPNIRGPEI VTM in isoform 2. CuratedVSP_041581

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ429216 Genomic DNA. Translation: CAD22137.1.
AJ300738 mRNA. Translation: CAC83350.1.
AY261383 Genomic DNA. Translation: AAO33581.1.
CCDSiCCDS37547.1. [Q8K1T0-1]
CCDS50053.1. [Q8K1T0-2]
RefSeqiNP_542765.2. NM_080727.2. [Q8K1T0-1]
UniGeneiMm.214638.

Genome annotation databases

EnsembliENSMUST00000024833; ENSMUSP00000024833; ENSMUSG00000024034. [Q8K1T0-1]
ENSMUST00000114549; ENSMUSP00000110196; ENSMUSG00000024034. [Q8K1T0-2]
GeneIDi140765.
KEGGimmu:140765.
UCSCiuc008bup.2. mouse. [Q8K1T0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ429216 Genomic DNA. Translation: CAD22137.1.
AJ300738 mRNA. Translation: CAC83350.1.
AY261383 Genomic DNA. Translation: AAO33581.1.
CCDSiCCDS37547.1. [Q8K1T0-1]
CCDS50053.1. [Q8K1T0-2]
RefSeqiNP_542765.2. NM_080727.2. [Q8K1T0-1]
UniGeneiMm.214638.

3D structure databases

ProteinModelPortaliQ8K1T0.
SMRiQ8K1T0. Positions 108-448.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.079.

PTM databases

PhosphoSiteiQ8K1T0.

Proteomic databases

PRIDEiQ8K1T0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024833; ENSMUSP00000024833; ENSMUSG00000024034. [Q8K1T0-1]
ENSMUST00000114549; ENSMUSP00000110196; ENSMUSG00000024034. [Q8K1T0-2]
GeneIDi140765.
KEGGimmu:140765.
UCSCiuc008bup.2. mouse. [Q8K1T0-1]

Organism-specific databases

CTDi64699.
MGIiMGI:2155445. Tmprss3.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118962.
HOGENOMiHOG000251822.
HOVERGENiHBG013304.
InParanoidiQ8K1T0.
KOiK09634.
OMAiVITPLWI.
OrthoDBiEOG75B84T.
TreeFamiTF351678.

Miscellaneous databases

NextBioi369973.
PROiQ8K1T0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K1T0.
CleanExiMM_TMPRSS3.
ExpressionAtlasiQ8K1T0. baseline and differential.
GenevestigatoriQ8K1T0.

Family and domain databases

Gene3Di3.10.250.10. 1 hit.
4.10.400.10. 1 hit.
InterProiIPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00057. Ldl_recept_a. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00192. LDLa. 1 hit.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 1 hit.
PROSITEiPS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SUBCELLULAR LOCATION, FUNCTION IN ENAC CLEAVAGE.
  2. "Genomic organization of murine transmembrane proteinases."
    Rao N.V., Rao G.N., Hoidal J.R.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Tmprss3, a transmembrane serine protease deficient in human DFNB8/10 deafness, is critical for cochlear hair cell survival at the onset of hearing."
    Fasquelle L., Scott H.S., Lenoir M., Wang J., Rebillard G., Gaboyard S., Venteo S., Francois F., Mausset-Bonnefont A.L., Antonarakis S.E., Neidhart E., Chabbert C., Puel J.L., Guipponi M., Delprat B.
    J. Biol. Chem. 286:17383-17397(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE SPLICING (ISOFORM 2).

Entry informationi

Entry nameiTMPS3_MOUSE
AccessioniPrimary (citable) accession number: Q8K1T0
Secondary accession number(s): Q812A6, Q8VDE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2003
Last sequence update: July 27, 2011
Last modified: February 4, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.