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Protein

Protein spire homolog 2

Gene

Spire2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning and asymmetric cell division during oocyte meiosis. Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis.2 Publications

GO - Biological processi

  • actin cytoskeleton organization Source: UniProtKB
  • actin nucleation Source: InterPro
  • cleavage furrow formation Source: UniProtKB
  • establishment of meiotic spindle localization Source: UniProtKB
  • formin-nucleated actin cable assembly Source: UniProtKB
  • intracellular transport Source: UniProtKB
  • polar body extrusion after meiotic divisions Source: UniProtKB
  • protein transport Source: UniProtKB-KW
  • vesicle-mediated transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein spire homolog 2
Short name:
Spir-2
Gene namesi
Name:Spire2
Synonyms:spir-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2446256. Spire2.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: UniProtKB
  • cytoplasmic vesicle membrane Source: UniProtKB
  • cytoskeleton Source: UniProtKB-SubCell
  • cytosol Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 718718Protein spire homolog 2PRO_0000320024Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei374 – 3741PhosphoserineCombined sources
Modified residuei443 – 4431PhosphoserineBy similarity
Modified residuei445 – 4451PhosphoserineBy similarity
Modified residuei479 – 4791PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8K1S6.
PaxDbiQ8K1S6.
PRIDEiQ8K1S6.

PTM databases

iPTMnetiQ8K1S6.
PhosphoSiteiQ8K1S6.

Expressioni

Tissue specificityi

Detected in oocytes.1 Publication

Gene expression databases

BgeeiQ8K1S6.
CleanExiMM_SPIRE2.
GenevisibleiQ8K1S6. MM.

Interactioni

Protein-protein interaction databases

BioGridi231590. 32 interactions.
IntActiQ8K1S6. 32 interactions.
STRINGi10090.ENSMUSP00000010298.

Structurei

3D structure databases

ProteinModelPortaliQ8K1S6.
SMRiQ8K1S6. Positions 26-211.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 207182KINDPROSITE-ProRule annotationAdd
BLAST
Domaini251 – 26515WH2 1Add
BLAST
Domaini281 – 29919WH2 2Add
BLAST
Domaini345 – 36218WH2 3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni538 – 55821Spir-boxAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi576 – 60328Cys-richAdd
BLAST

Domaini

Binds to actin monomers via the WH2 domain.By similarity
The Spir-box targets binding to intracellular membrane structures.By similarity

Sequence similaritiesi

Belongs to the spire family.Curated
Contains 1 KIND domain.PROSITE-ProRule annotation
Contains 3 WH2 domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IK9H. Eukaryota.
ENOG4111E0J. LUCA.
GeneTreeiENSGT00390000003058.
HOGENOMiHOG000013039.
HOVERGENiHBG058898.
InParanoidiQ8K1S6.
KOiK02098.
OMAiCGAADEG.
OrthoDBiEOG77DJ5G.
PhylomeDBiQ8K1S6.
TreeFamiTF326239.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR011019. KIND_dom.
IPR029901. Spire.
IPR029904. Spire2.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR21345. PTHR21345. 2 hits.
PTHR21345:SF5. PTHR21345:SF5. 2 hits.
PfamiPF16474. KIND. 1 hit.
[Graphical view]
SMARTiSM00750. KIND. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS51377. KIND. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K1S6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARAGGGGAA APERAGGAAR PEPWELSLEE VLKVYEQPIN EEQAWAVCFQ
60 70 80 90 100
GCRGLRGEPG GVRRIRDTAD ILLRRDGSVG ARLEPEPTTM VVPPASSEAQ
110 120 130 140 150
MVQSLGFAIY RALDWGLDEN EERELSPQLE RLIDLMANSD CEDSSCGAAD
160 170 180 190 200
EGYVGPEEEE EAEGGPRAVR TFAQAMRLCA LRLTDPHGAQ AHYQAVCRAL
210 220 230 240 250
FVETLELRAF LARVREAKEM LKKLGEEEPR EKPLAELDHL GHTDWARLWV
260 270 280 290 300
QLMRELRHGV KLKKVQEKEF NPLPTEFQLT PFEMLMQDIR ARNYKLRKVM
310 320 330 340 350
VDGDIPPRVK KDAHELILDF IRSRPPLKQV SERQLRPVPQ KQRTLHEKIL
360 370 380 390 400
EEIKQERRLR PVGAQHLGGR GFGSLPCILN ACSGDIKSTS CINLSVTDTG
410 420 430 440 450
SGSQRPRPRV LLKAPTLAEM EEMNTSEEEE SPCGEVALKR DRSFSEHDLA
460 470 480 490 500
QLRSEMASGL QSAAQPPGGT EPPRARAGSM HSWRPSSRDQ GFCPVSGQSQ
510 520 530 540 550
PLPSSALPSS LSSVDGPEAA SPDTRHLWLE FSHPVESLAL TVEEVVDVRR
560 570 580 590 600
VLVKAEMERF LQDKELFSSL KRGKICCCCR AKFPLFSWPP TCLFCKRAVC
610 620 630 640 650
TSCSVKMKMP SKKYGHIPVY TLGFESLQRV PTTKATPTLR RDAFQSLQGP
660 670 680 690 700
KWRSVEEEFP HIYAHGCVLK DVCSDCTSFV ADVVCSSRKS VDVLNATPRR
710
SRQTQSLYIP NTRTLNFQ
Length:718
Mass (Da):80,210
Last modified:October 1, 2002 - v1
Checksum:i3D6B61292594E9B0
GO
Isoform 2 (identifier: Q8K1S6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.

Show »
Length:618
Mass (Da):69,603
Checksum:iB70590C54C0D6893
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 100100Missing in isoform 2. 1 PublicationVSP_031573Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ459115 Genomic DNA. Translation: CAD30509.1.
BC026502 mRNA. Translation: AAH26502.1.
BC049152 mRNA. Translation: AAH49152.1.
CCDSiCCDS22754.1. [Q8K1S6-1]
RefSeqiNP_758491.1. NM_172287.2. [Q8K1S6-1]
XP_006531026.1. XM_006530963.2. [Q8K1S6-2]
XP_011246677.1. XM_011248375.1. [Q8K1S6-2]
UniGeneiMm.150200.

Genome annotation databases

EnsembliENSMUST00000010298; ENSMUSP00000010298; ENSMUSG00000010154. [Q8K1S6-1]
GeneIDi234857.
KEGGimmu:234857.
UCSCiuc009nvj.1. mouse. [Q8K1S6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ459115 Genomic DNA. Translation: CAD30509.1.
BC026502 mRNA. Translation: AAH26502.1.
BC049152 mRNA. Translation: AAH49152.1.
CCDSiCCDS22754.1. [Q8K1S6-1]
RefSeqiNP_758491.1. NM_172287.2. [Q8K1S6-1]
XP_006531026.1. XM_006530963.2. [Q8K1S6-2]
XP_011246677.1. XM_011248375.1. [Q8K1S6-2]
UniGeneiMm.150200.

3D structure databases

ProteinModelPortaliQ8K1S6.
SMRiQ8K1S6. Positions 26-211.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231590. 32 interactions.
IntActiQ8K1S6. 32 interactions.
STRINGi10090.ENSMUSP00000010298.

PTM databases

iPTMnetiQ8K1S6.
PhosphoSiteiQ8K1S6.

Proteomic databases

MaxQBiQ8K1S6.
PaxDbiQ8K1S6.
PRIDEiQ8K1S6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000010298; ENSMUSP00000010298; ENSMUSG00000010154. [Q8K1S6-1]
GeneIDi234857.
KEGGimmu:234857.
UCSCiuc009nvj.1. mouse. [Q8K1S6-1]

Organism-specific databases

CTDi84501.
MGIiMGI:2446256. Spire2.

Phylogenomic databases

eggNOGiENOG410IK9H. Eukaryota.
ENOG4111E0J. LUCA.
GeneTreeiENSGT00390000003058.
HOGENOMiHOG000013039.
HOVERGENiHBG058898.
InParanoidiQ8K1S6.
KOiK02098.
OMAiCGAADEG.
OrthoDBiEOG77DJ5G.
PhylomeDBiQ8K1S6.
TreeFamiTF326239.

Miscellaneous databases

PROiQ8K1S6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K1S6.
CleanExiMM_SPIRE2.
GenevisibleiQ8K1S6. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR011019. KIND_dom.
IPR029901. Spire.
IPR029904. Spire2.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR21345. PTHR21345. 2 hits.
PTHR21345:SF5. PTHR21345:SF5. 2 hits.
PfamiPF16474. KIND. 1 hit.
[Graphical view]
SMARTiSM00750. KIND. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS51377. KIND. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Spir protein family."
    Kerkhoff E., Leberfinger C.B., Borawski J.M., Rapp U.R., Doerks T., Bork P.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Brain, Colon and Eye.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Eye and Olfactory epithelium.
  3. "Overlapping expression pattern of the actin organizers Spir-1 and formin-2 in the developing mouse nervous system and the adult brain."
    Schumacher N., Borawski J.M., Leberfinger C.B., Gessler M., Kerkhoff E.
    Gene Expr. Patterns 4:249-255(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SPIRE FAMILY.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-374, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.
  5. "Spire-type actin nucleators cooperate with Formin-2 to drive asymmetric oocyte division."
    Pfender S., Kuznetsov V., Pleiser S., Kerkhoff E., Schuh M.
    Curr. Biol. 21:955-960(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  6. "An actin-dependent mechanism for long-range vesicle transport."
    Schuh M.
    Nat. Cell Biol. 13:1431-1436(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSPIR2_MOUSE
AccessioniPrimary (citable) accession number: Q8K1S6
Secondary accession number(s): Q8R0R2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 1, 2002
Last modified: July 6, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.