Reviewed,
UniProtKB/Swiss-Prot Q8K1S3 (UNC5B_MOUSE)
Last modified
February 9, 2010.
Version 69.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Netrin receptor UNC5B Alternative name(s): Protein unc-5 homolog B Unc-5 homolog 2 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 945 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand By similarity. |
| Subunit structure | Interacts with the cytoplasmic part of DCC. Interacts with GNAI2 via its cytoplasmic part By similarity. |
| Subcellular location | Membrane; Single-pass type I membrane protein By similarity. |
| Tissue specificity | Highly expressed in brain. Expressed in lung during late development. Expressed during early blood vessel formation, in the semicircular canal and in a dorsal to ventral gradient in the retina. Ref.1 Ref.4 |
| Post-translational modification | Phosphorylated on cytoplasmic tyrosine residues By similarity. Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis By similarity. |
| Sequence similarities | Belongs to the unc-5 family. Contains 1 death domain. Contains 1 Ig-like (immunoglobulin-like) domain. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 2 TSP type-1 domains. Contains 1 ZU5 domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Apoptosis |
| Cellular component | Membrane |
| Coding sequence diversity | Alternative splicing |
| Domain | Immunoglobulin domain Repeat Signal Transmembrane |
| Molecular function | Developmental protein Receptor |
| PTM | Disulfide bond Glycoprotein Phosphoprotein |
| Technical term | 3D-structure |
| Gene Ontology (GO) | |
| Biological process | apoptosis Inferred from electronic annotation. Source: UniProtKB-KW signal transductionInferred from electronic annotation. Source: InterPro |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | protein binding Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q8K1S3-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q8K1S3-2) The sequence of this isoform differs from the canonical sequence as follows: 356-367: NQRTLNDPKSHP → T |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||||
Molecule processing | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 26 | 26 | Potential | ||||||||||||||||||||||
| Chain | 27 – 945 | 919 | Netrin receptor UNC5B | PRO_0000036072 | |||||||||||||||||||||
Regions | |||||||||||||||||||||||||
| Topological domain | 27 – 377 | 351 | Extracellular Potential | ||||||||||||||||||||||
| Transmembrane | 378 – 398 | 21 | Potential | ||||||||||||||||||||||
| Topological domain | 399 – 945 | 547 | Cytoplasmic Potential | ||||||||||||||||||||||
| Domain | 48 – 145 | 98 | Ig-like | ||||||||||||||||||||||
| Domain | 153 – 242 | 90 | Ig-like C2-type | ||||||||||||||||||||||
| Domain | 246 – 300 | 55 | TSP type-1 1 | ||||||||||||||||||||||
| Domain | 302 – 354 | 53 | TSP type-1 2 | ||||||||||||||||||||||
| Domain | 541 – 647 | 107 | ZU5 | ||||||||||||||||||||||
| Domain | 865 – 943 | 79 | Death | ||||||||||||||||||||||
| Region | 707 – 725 | 19 | Interaction with DCC By similarity | ||||||||||||||||||||||
Sites | |||||||||||||||||||||||||
| Site | 412 – 413 | 2 | Cleavage; by caspase-3 By similarity | ||||||||||||||||||||||
Amino acid modifications | |||||||||||||||||||||||||
| Glycosylation | 222 | 1 | N-linked (GlcNAc...) Potential | ||||||||||||||||||||||
| Glycosylation | 347 | 1 | N-linked (GlcNAc...) Potential | ||||||||||||||||||||||
| Disulfide bond | 69 ↔ 128 | By similarity | |||||||||||||||||||||||
| Disulfide bond | 174 ↔ 225 | By similarity | |||||||||||||||||||||||
| Disulfide bond | 258 ↔ 295 | By similarity | |||||||||||||||||||||||
| Disulfide bond | 262 ↔ 299 | By similarity | |||||||||||||||||||||||
| Disulfide bond | 273 ↔ 285 | By similarity | |||||||||||||||||||||||
Natural variations | |||||||||||||||||||||||||
| Alternative sequence | 356 – 367 | 12 | NQRTL…PKSHP → T in isoform 2. | VSP_011699 | |||||||||||||||||||||
Experimental info | |||||||||||||||||||||||||
| Sequence conflict | 238 | 1 | T → A in BAB31108. Ref.2 | ||||||||||||||||||||||
| Sequence conflict | 394 | 1 | V → E in BAB31108. Ref.2 | ||||||||||||||||||||||
| Sequence conflict | 679 | 1 | T → S in BAB31108. Ref.2 | ||||||||||||||||||||||
| Sequence conflict | 874 | 1 | N → D in BAB31108. Ref.2 | ||||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||||
| Helix | 860 – 871 | 12 | |||||||||||||||||||||||
| Helix | 873 – 875 | 3 | |||||||||||||||||||||||
| Helix | 880 – 886 | 7 | |||||||||||||||||||||||
| Helix | 890 – 892 | 3 | |||||||||||||||||||||||
| Helix | 893 – 897 | 5 | |||||||||||||||||||||||
| Helix | 902 – 913 | 12 | |||||||||||||||||||||||
| Turn | 917 – 919 | 3 | |||||||||||||||||||||||
| Helix | 920 – 929 | 10 | |||||||||||||||||||||||
| Helix | 933 – 941 | 9 | |||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning of three mouse Unc5 genes and their expression patterns at mid-gestation." Engelkamp D. Mech. Dev. 118:191-197(2002) [PubMed: 12351186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. |
| [2] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J and NOD. Tissue: Medulla oblongata. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Brain. |
| [4] | "Expression of Netrin-1 and its two receptors DCC and UNC5H2 in the developing mouse lung." Dalvin S., Anselmo M.A., Prodhan P., Komatsuzaki K., Schnitzer J.J., Kinane T.B. Gene Expr. Patterns 3:279-283(2003) [PubMed: 12799072] [Abstract] Cited for: TISSUE SPECIFICITY. |
| [5] | "Crystal structure of the UNC5H2 death domain." RIKEN structural genomics initiative (RSGI) Submitted (JAN-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 854-943. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | AJ487853 mRNA. Translation: CAD32251.1. AK018177 mRNA. Translation: BAB31108.1. AK154271 mRNA. Translation: BAE32479.1. BC048162 mRNA. Translation: AAH48162.1. Different initiation. BC057560 mRNA. Translation: AAH57560.1. | ||||||||||||
| IPI | IPI00378122. IPI00471418. | ||||||||||||
| RefSeq | NP_084046.2. | ||||||||||||
| UniGene | Mm.290433 | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
| ||||||||||||
| SMR | Q8K1S3. Positions 246-353. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| STRING | Q8K1S3. | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | Q8K1S3. | ||||||||||||
Proteomic databases | |||||||||||||
| PRIDE | Q8K1S3. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSMUST00000077925; ENSMUSP00000077080; ENSMUSG00000020099; Mus musculus. [Genome view] ENSMUST00000105458; ENSMUSP00000101098; ENSMUSG00000020099; Mus musculus. [Genome view] | ||||||||||||
| GeneID | 107449. | ||||||||||||
| KEGG | mmu:107449. | ||||||||||||
| UCSC | uc007ffe.1. mouse. uc007fff.1. mouse. | ||||||||||||
Organism-specific databases | |||||||||||||
| CTD | 107449. | ||||||||||||
| MGI | MGI:894703. Unc5b. | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | HBG358171. | ||||||||||||
| HOVERGEN | Q8K1S3. | ||||||||||||
| InParanoid | Q8K1S3. | ||||||||||||
| OMA | IFAPALC. | ||||||||||||
| PhylomeDB | Q8K1S3. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | Q8K1S3. | ||||||||||||
| Bgee | Q8K1S3. | ||||||||||||
| CleanEx | MM_UNC5B. | ||||||||||||
| Genevestigator | Q8K1S3. | ||||||||||||
| GermOnline | ENSMUSG00000020099. Mus musculus. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR000488. Death. IPR011029. DEATH-like. IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR003598. Ig_sub2. IPR000884. Thrombospondin_1_rpt. IPR008085. Thrombospondin_1_rpt_sub. IPR000906. ZU5. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:1.10.533.10. DEATH_like. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits. | ||||||||||||
| Pfam | PF00531. Death. 1 hit. PF00090. TSP_1. 2 hits. PF00791. ZU5. 1 hit. [Graphical view] | ||||||||||||
| PRINTS | PR01705. TSP1REPEAT. | ||||||||||||
| SMART | SM00005. DEATH. 1 hit. SM00408. IGc2. 1 hit. SM00209. TSP1. 2 hits. SM00218. ZU5. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS50017. DEATH_DOMAIN. 1 hit. PS50835. IG_LIKE. 1 hit. PS50092. TSP1. 2 hits. PS51145. ZU5. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| NextBio | 358822. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | UNC5B_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q8K1S3 Secondary accession number(s): Q3U4F2 Q9D398 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


