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Q8K1S3 (UNC5B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Netrin receptor UNC5B
Alternative name(s):
Protein unc-5 homolog 2
Protein unc-5 homolog B
Gene names
Name:Unc5b
Synonyms:Unc5h2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length945 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand By similarity. Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity By similarity. Ref.5

Subunit structure

Interacts with the cytoplasmic part of DCC. Interacts with GNAI2 via its cytoplasmic part By similarity. Interacts (via death domain) with DAPK1 (via death domain) By similarity. Ref.5

Subcellular location

Membrane; Single-pass type I membrane protein By similarity. Note: Associated with lipid raft.

Tissue specificity

Highly expressed in brain. Expressed in lung during late development. Expressed during early blood vessel formation, in the semicircular canal and in a dorsal to ventral gradient in the retina. Ref.1 Ref.4

Post-translational modification

Phosphorylated on cytoplasmic tyrosine residues By similarity.

Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis By similarity.

Palmitoylation target the protein to the lipid rafts, and is required for pro-apoptotic activity By similarity.

Sequence similarities

Belongs to the unc-5 family.

Contains 1 death domain.

Contains 1 Ig-like (immunoglobulin-like) domain.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 2 TSP type-1 domains.

Contains 1 ZU5 domain.

Sequence caution

The sequence AAH48162.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Ppp2r1bQ7TNP22EBI-4396886,EBI-4396871

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8K1S3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8K1S3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     356-367: NQRTLNDPKSHP → T

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 945919Netrin receptor UNC5B
PRO_0000036072

Regions

Topological domain27 – 377351Extracellular Potential
Transmembrane378 – 39821Helical; Potential
Topological domain399 – 945547Cytoplasmic Potential
Domain48 – 14598Ig-like
Domain153 – 24290Ig-like C2-type
Domain246 – 30055TSP type-1 1
Domain302 – 35453TSP type-1 2
Domain544 – 688145ZU5
Domain865 – 94379Death
Region689 – 838150UPA domain By similarity
Region707 – 72519Interaction with DCC By similarity

Sites

Site412 – 4132Cleavage; by caspase-3 By similarity

Amino acid modifications

Lipidation4031S-palmitoyl cysteine By similarity
Glycosylation2221N-linked (GlcNAc...) Potential
Glycosylation3471N-linked (GlcNAc...) Potential
Disulfide bond69 ↔ 128 By similarity
Disulfide bond174 ↔ 225 By similarity
Disulfide bond258 ↔ 295 By similarity
Disulfide bond262 ↔ 299 By similarity
Disulfide bond273 ↔ 285 By similarity

Natural variations

Alternative sequence356 – 36712NQRTL…PKSHP → T in isoform 2.
VSP_011699

Experimental info

Sequence conflict2381T → A in BAB31108. Ref.2
Sequence conflict3941V → E in BAB31108. Ref.2
Sequence conflict6791T → S in BAB31108. Ref.2
Sequence conflict8741N → D in BAB31108. Ref.2

Secondary structure

................. 945
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 80E896F0F0E06012

FASTA945103,739
        10         20         30         40         50         60 
MRARSGVRSA LLLALLLCWD PTPSLAGVDS AGQVLPDSYP SAPAEQLPYF LLEPQDAYIV 

        70         80         90        100        110        120 
KNKPVELHCR AFPATQIYFK CNGEWVSQND HVTQESLDEA TGLRVREVQI EVSRQQVEEL 

       130        140        150        160        170        180 
FGLEDYWCQC VAWSSSGTTK SRRAYIRIAY LRKNFDQEPL AKEVPLDHEV LLQCRPPEGV 

       190        200        210        220        230        240 
PVAEVEWLKN EDVIDPAQDT NFLLTIDHNL IIRQARLSDT ANYTCVAKNI VAKRRSTTAT 

       250        260        270        280        290        300 
VIVYVNGGWS SWAEWSPCSN RCGRGWQKRT RTCTNPAPLN GGAFCEGQAF QKTACTTVCP 

       310        320        330        340        350        360 
VDGAWTEWSK WSACSTECAH WRSRECMAPP PQNGGRDCSG TLLDSKNCTD GLCVLNQRTL 

       370        380        390        400        410        420 
NDPKSHPLET SGDVALYAGL VVAVFVVVAV LMAVGVIVYR RNCRDFDTDI TDSSAALTGG 

       430        440        450        460        470        480 
FHPVNFKTAR PNNPQLLHPS APPDLTASAG IYRGPVYALQ DSADKIPMTN SPLLDPLPSL 

       490        500        510        520        530        540 
KIKVYNSSTI GSGSGLADGA DLLGVLPPGT YPGDFSRDTH FLHLRSASLG SQHLLGLPRD 

       550        560        570        580        590        600 
PSSSVSGTFG CLGGRLSLPG TGVSLLVPNG AIPQGKFYDL YLHINKAEST LPLSEGSQTV 

       610        620        630        640        650        660 
LSPSVTCGPT GLLLCRPVVL TVPHCAEVIA GDWIFQLKTQ AHQGHWEEVV TLDEETLNTP 

       670        680        690        700        710        720 
CYCQLEAKSC HILLDQLGTY VFMGESYSRS AVKRLQLAIF APALCTSLEY SLRVYCLEDT 

       730        740        750        760        770        780 
PVALKEVLEL ERTLGGYLVE EPKPLLFKDS YHNLRLSLHD IPHAHWRSKL LAKYQEIPFY 

       790        800        810        820        830        840 
HVWNGSQRAL HCTFTLERHS LASTEFTCKV CVRQVEGEGQ IFQLHTTLAE TPAGSLDALC 

       850        860        870        880        890        900 
SAPGNAITTQ LGPYAFKIPL SIRQKICSSL DAPNSRGNDW RLLAQKLSMD RYLNYFATKA 

       910        920        930        940 
SPTGVILDLW EARQQDDGDL NSLASALEEM GKSEMLVAMA TDGDC 

« Hide

Isoform 2 [UniParc].

Checksum: E92DB6288F013C98
Show »

FASTA934102,452

References

« Hide 'large scale' references
[1]"Cloning of three mouse Unc5 genes and their expression patterns at mid-gestation."
Engelkamp D.
Mech. Dev. 118:191-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J and NOD.
Tissue: Medulla oblongata.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Brain.
[4]"Expression of Netrin-1 and its two receptors DCC and UNC5H2 in the developing mouse lung."
Dalvin S., Anselmo M.A., Prodhan P., Komatsuzaki K., Schnitzer J.J., Kinane T.B.
Gene Expr. Patterns 3:279-283(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"The dependence receptor UNC5H2 mediates apoptosis through DAP-kinase."
Llambi F., Lourenco F.C., Gozuacik D., Guix C., Pays L., Del Rio G., Kimchi A., Mehlen P.
EMBO J. 24:1192-1201(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH DAPK1.
[6]"Structure of the UNC5H2 death domain."
Handa N., Kukimoto-Niino M., Akasaka R., Murayama K., Terada T., Inoue M., Yabuki T., Aoki M., Seki E., Matsuda T., Nunokawa E., Tanaka A., Hayashizaki Y., Kigawa T., Shirouzu M., Yokoyama S.
Acta Crystallogr. D 62:1502-1509(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 854-943.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ487853 mRNA. Translation: CAD32251.1.
AK018177 mRNA. Translation: BAB31108.1.
AK154271 mRNA. Translation: BAE32479.1.
BC048162 mRNA. Translation: AAH48162.1. Different initiation.
BC057560 mRNA. Translation: AAH57560.1.
IPIIPI00378122.
IPI00471418.
RefSeqNP_084046.2. NM_029770.2.
UniGeneMm.290433.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WMGX-ray2.10A/B/C/D/E/F854-943[»]
ProteinModelPortalQ8K1S3.
SMRQ8K1S3. Positions 46-353, 541-943.
ModBaseSearch...

Protein-protein interaction databases

IntActQ8K1S3. 2 interactions.

PTM databases

PhosphoSiteQ8K1S3.

Proteomic databases

PaxDbQ8K1S3.
PRIDEQ8K1S3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000077925; ENSMUSP00000077080; ENSMUSG00000020099.
GeneID107449.
KEGGmmu:107449.
UCSCuc007ffd.1. mouse.
uc007ffe.1. mouse.

Organism-specific databases

CTD219699.
MGIMGI:894703. Unc5b.

Phylogenomic databases

eggNOGNOG293019.
GeneTreeENSGT00640000091083.
HOGENOMHOG000060306.
HOVERGENHBG056483.
InParanoidQ8K1S3.
KOK07521.
OMAHEVLLQC.
OrthoDBEOG4ZW598.

Enzyme and pathway databases

ReactomeREACT_100962. Apoptosis.

Gene expression databases

ArrayExpressQ8K1S3.
BgeeQ8K1S3.
CleanExMM_UNC5B.
GenevestigatorQ8K1S3.
GermOnlineENSMUSG00000020099. Mus musculus.

Family and domain databases

Gene3D1.10.533.10. 1 hit.
2.60.40.10. 2 hits.
InterProIPR011029. DEATH-like_dom.
IPR000488. Death_domain.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
IPR000884. Thrombospondin_1_rpt.
IPR000906. ZU5.
[Graphical view]
PfamPF00531. Death. 1 hit.
PF07679. I-set. 1 hit.
PF00090. TSP_1. 2 hits.
PF00791. ZU5. 1 hit.
[Graphical view]
SMARTSM00005. DEATH. 1 hit.
SM00408. IGc2. 1 hit.
SM00209. TSP1. 2 hits.
SM00218. ZU5. 1 hit.
[Graphical view]
SUPFAMSSF47986. DEATH_like. 1 hit.
SSF82895. TSP1. 2 hits.
PROSITEPS50017. DEATH_DOMAIN. 1 hit.
PS50835. IG_LIKE. 1 hit.
PS50092. TSP1. 2 hits.
PS51145. ZU5. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSUNC5B. mouse.
EvolutionaryTraceQ8K1S3.
NextBio358822.
SOURCESearch...

Entry information

Entry nameUNC5B_MOUSE
AccessionPrimary (citable) accession number: Q8K1S3
Secondary accession number(s): Q3U4F2 expand/collapse secondary AC list , Q6PFH0, Q80Y85, Q9D398
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 1, 2002
Last modified: April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families