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Protein

Serine/threonine-protein kinase Nek9

Gene

Nek9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei81 – 811ATPPROSITE-ProRule annotation
Active sitei176 – 1761Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi58 – 669ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-MMU-3301854. Nuclear Pore Complex (NPC) Disassembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Nek9 (EC:2.7.11.1)
Alternative name(s):
Nercc1 kinase
Never in mitosis A-related kinase 9
Short name:
NimA-related protein kinase 9
Gene namesi
Name:Nek9
Synonyms:Nercc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2387995. Nek9.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 984983Serine/threonine-protein kinase Nek9PRO_0000086436Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei13 – 131PhosphoserineBy similarity
Modified residuei16 – 161PhosphoserineBy similarity
Modified residuei20 – 201PhosphoserineBy similarity
Modified residuei52 – 521PhosphotyrosineBy similarity
Modified residuei76 – 761PhosphoserineBy similarity
Modified residuei210 – 2101Phosphothreonine; by autocatalysisBy similarity
Modified residuei254 – 2541PhosphothreonineBy similarity
Modified residuei331 – 3311PhosphoserineCombined sources
Modified residuei333 – 3331PhosphothreonineBy similarity
Modified residuei741 – 7411PhosphoserineBy similarity
Modified residuei808 – 8081PhosphoserineBy similarity
Modified residuei839 – 8391PhosphoserineBy similarity
Modified residuei891 – 8911PhosphothreonineBy similarity
Modified residuei949 – 9491PhosphoserineBy similarity
Modified residuei983 – 9831PhosphoserineBy similarity

Post-translational modificationi

Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8K1R7.
MaxQBiQ8K1R7.
PaxDbiQ8K1R7.
PRIDEiQ8K1R7.

PTM databases

iPTMnetiQ8K1R7.
PhosphoSiteiQ8K1R7.

Expressioni

Gene expression databases

BgeeiQ8K1R7.
GenevisibleiQ8K1R7. MM.

Interactioni

Subunit structurei

Homodimer. Binds to Ran GTPase. Has a greater affinity for Ran-GDP over Ran-GTP. Interacts with NEK6, NEK7 and BICD2. Interacts with SSRP1 and SUPT16H, the 2 subunits of the FACT complex. Interacts with DYNLL1; phosphorylation at Ser-949 strongly reduces DYNLL1 binding (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229954. 26 interactions.
DIPiDIP-56880N.
IntActiQ8K1R7. 26 interactions.
MINTiMINT-4103245.
STRINGi10090.ENSMUSP00000049056.

Structurei

Secondary structure

1
984
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi818 – 8247Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5DE2X-ray2.78C/D817-835[»]
ProteinModelPortaliQ8K1R7.
SMRiQ8K1R7. Positions 51-725.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini52 – 308257Protein kinasePROSITE-ProRule annotationAdd
BLAST
Repeati388 – 44457RCC1 1Add
BLAST
Repeati445 – 49854RCC1 2Add
BLAST
Repeati499 – 55052RCC1 3Add
BLAST
Repeati551 – 61565RCC1 4Add
BLAST
Repeati616 – 66853RCC1 5Add
BLAST
Repeati669 – 72658RCC1 6Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni732 – 896165Interaction with NEK6By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili896 – 94550Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi752 – 76716Poly-GlyAdd
BLAST
Compositional biasi772 – 893122Pro/Ser/Thr-richAdd
BLAST

Domaini

Dimerizes through its coiled-coil domain.By similarity

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 6 RCC1 repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0589. Eukaryota.
KOG1426. Eukaryota.
ENOG410Y7JF. LUCA.
GeneTreeiENSGT00760000118997.
HOVERGENiHBG039572.
InParanoidiQ8K1R7.
KOiK08857.
OMAiPTEAMGN.
OrthoDBiEOG7G7KNB.
TreeFamiTF106472.

Family and domain databases

Gene3Di2.130.10.30. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR009091. RCC1/BLIP-II.
IPR000408. Reg_chr_condens.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00415. RCC1. 4 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50985. SSF50985. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50012. RCC1_3. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K1R7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVLGEYERH CDSINSDFGS ESGGGGDSGP GPSAVPGPRA GGGAAEQEEL
60 70 80 90 100
HYIPIRVLGR GAFGEATLYR RTEDDSLVVW KEVDLTRLSE KERRDALNEI
110 120 130 140 150
VILALLQHDN IIAYYNHFMD NTTLLIELEY CNGGNLYDKI LRQKDKLFEE
160 170 180 190 200
EMVVWYLFQI VSAVSCIHKA GILHRDIKTL NIFLTKANLI KLGDYGLAKK
210 220 230 240 250
LNSEYSMAET LVGTPYYMSP ELCQGVKYNF KSDIWAVGCV IFELLTLKRT
260 270 280 290 300
FDATNPLNLC VKIVQGIRAM EVDSSQYSLE LIQLVHACLD QDPEQRPAAD
310 320 330 340 350
ALLDLPLLRT RRREMEEKVT LLNAPTKRPR SSTVTEAPIA VVTSRTSEVY
360 370 380 390 400
VWGGGKSTPQ KLDVIKSGCS ARQVCAGNTH FAVVTVEKEL YTWVNMQGGT
410 420 430 440 450
KLHGQLGHGD KASYRQPKHV EKLQGKAIHQ VSCGDDFTVC VTDEGQLYAF
460 470 480 490 500
GSDYYGCMGV DKVSGPEVLE PMQLNFFLSN PVEQVSCGDN HVVVLTRNKE
510 520 530 540 550
VYSWGCGEYG RLGLDSEEDY YTPQRVDVPK ALIIVAVQCG CDGTFLLTQS
560 570 580 590 600
GKVLACGLNE FNKLGLNQCM SGIINHEAYH EVPYTTSFTL AKQLSFYKIR
610 620 630 640 650
TIAPGKTHTA AIDERGRLLT FGCNKCGQLG VGNYKKRLGI NLLGGPLGGK
660 670 680 690 700
QVIRVSCGDE FTIAATDDNH IFAWGNGGNG RLAMTPTERP HGSDICTSWP
710 720 730 740 750
RPIFGSLHHV PDLSCRGWHT ILIVEKVLNS KTIRSNSSGL SIGTVVQSSS
760 770 780 790 800
PGGGIGGGGG GGGGGGGEEE DSQQESETPD PSGGFRGTME ADRGMEGLIS
810 820 830 840 850
PTEAVGNSCG ASSSCPGWLR KELENAEFIP MPDSPAPLSA AFSQSEKDTL
860 870 880 890 900
PYEELQGLKV ASEVPPEPQR AAGAWPPRLD PAVPCVGKAL TSAACACSAL
910 920 930 940 950
QVEVDRLQAL VLKCLEEQQK LQQENLQMFT QLQKLNKKLE GGQQVGMHSR
960 970 980
GTQTAKEEME MDPKPDLDSE SWCLLGTDSC RPSL
Length:984
Mass (Da):107,143
Last modified:July 27, 2011 - v2
Checksum:i034A33BF04667487
GO

Sequence cautioni

The sequence AAH24926.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti929 – 9291F → S in CAD34025 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ489828 mRNA. Translation: CAD34025.1.
CH466590 Genomic DNA. Translation: EDL02865.1.
BC024926 mRNA. Translation: AAH24926.1. Different initiation.
BC117971 mRNA. Translation: AAI17972.1.
BC117972 mRNA. Translation: AAI17973.1.
CCDSiCCDS26057.1.
RefSeqiNP_660120.2. NM_145138.2.
UniGeneiMm.29071.

Genome annotation databases

EnsembliENSMUST00000040992; ENSMUSP00000049056; ENSMUSG00000034290.
GeneIDi217718.
KEGGimmu:217718.
UCSCiuc007ogx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ489828 mRNA. Translation: CAD34025.1.
CH466590 Genomic DNA. Translation: EDL02865.1.
BC024926 mRNA. Translation: AAH24926.1. Different initiation.
BC117971 mRNA. Translation: AAI17972.1.
BC117972 mRNA. Translation: AAI17973.1.
CCDSiCCDS26057.1.
RefSeqiNP_660120.2. NM_145138.2.
UniGeneiMm.29071.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5DE2X-ray2.78C/D817-835[»]
ProteinModelPortaliQ8K1R7.
SMRiQ8K1R7. Positions 51-725.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229954. 26 interactions.
DIPiDIP-56880N.
IntActiQ8K1R7. 26 interactions.
MINTiMINT-4103245.
STRINGi10090.ENSMUSP00000049056.

PTM databases

iPTMnetiQ8K1R7.
PhosphoSiteiQ8K1R7.

Proteomic databases

EPDiQ8K1R7.
MaxQBiQ8K1R7.
PaxDbiQ8K1R7.
PRIDEiQ8K1R7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040992; ENSMUSP00000049056; ENSMUSG00000034290.
GeneIDi217718.
KEGGimmu:217718.
UCSCiuc007ogx.2. mouse.

Organism-specific databases

CTDi91754.
MGIiMGI:2387995. Nek9.

Phylogenomic databases

eggNOGiKOG0589. Eukaryota.
KOG1426. Eukaryota.
ENOG410Y7JF. LUCA.
GeneTreeiENSGT00760000118997.
HOVERGENiHBG039572.
InParanoidiQ8K1R7.
KOiK08857.
OMAiPTEAMGN.
OrthoDBiEOG7G7KNB.
TreeFamiTF106472.

Enzyme and pathway databases

ReactomeiR-MMU-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-MMU-3301854. Nuclear Pore Complex (NPC) Disassembly.

Miscellaneous databases

PROiQ8K1R7.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K1R7.
GenevisibleiQ8K1R7. MM.

Family and domain databases

Gene3Di2.130.10.30. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR009091. RCC1/BLIP-II.
IPR000408. Reg_chr_condens.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00415. RCC1. 4 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50985. SSF50985. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50012. RCC1_3. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel NimA related protein kinase containing an RCC1-like domain."
    Joch M.R., Mandalfino C., Scime A., Whyte P.
    Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Heart.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-331, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiNEK9_MOUSE
AccessioniPrimary (citable) accession number: Q8K1R7
Secondary accession number(s): Q148U2, Q8R3P1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.