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Q8K1Q3 (GPR3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G-protein coupled receptor 3
Alternative name(s):
G-protein-coupled receptor R4
Gene names
Name:Gpr3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Orphan receptor with constitutive G(s) signaling activity that activate cyclic AMP. Has a potential role in modulating a number of brain functions, including behavioral responses to stress By similarity, amyloid-beta peptide generation in neurons By similarity and neurite outgrowth. Maintains also meiotic arrest in oocytes By similarity. Ref.4

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Abundantly expressed in granule neurons at all development stages. Ref.4

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence caution

The sequence CAD20634.1 differs from that shown. Reason: Frameshift at positions 210, 224 and 225.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329G-protein coupled receptor 3
PRO_0000379597

Regions

Topological domain1 – 4343Extracellular Potential
Transmembrane44 – 6421Helical; Name=1; Potential
Topological domain65 – 739Cytoplasmic Potential
Transmembrane74 – 9421Helical; Name=2; Potential
Topological domain95 – 10814Extracellular Potential
Transmembrane109 – 12921Helical; Name=3; Potential
Topological domain130 – 15324Cytoplasmic Potential
Transmembrane154 – 17421Helical; Name=4; Potential
Topological domain175 – 19218Extracellular Potential
Transmembrane193 – 21321Helical; Name=5; Potential
Topological domain214 – 24734Cytoplasmic Potential
Transmembrane248 – 26821Helical; Name=6; Potential
Topological domain269 – 2779Extracellular Potential
Transmembrane278 – 29821Helical; Name=7; Potential
Topological domain299 – 32931Cytoplasmic Potential
Compositional bias311 – 3144Poly-Cys

Amino acid modifications

Modified residue3231Phosphoserine Potential
Modified residue3251Phosphoserine Potential
Modified residue3271Phosphoserine Potential
Lipidation3121S-palmitoyl cysteine By similarity
Glycosylation201N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict611V → M in AAA73559. Ref.2
Sequence conflict1171T → M in AAA73559. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8K1Q3 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: 96E347B2059BD85A

FASTA32935,327
        10         20         30         40         50         60 
MMWGAGRSMA WFSAGSGSVN VSIDPAEEPT GPATLLPSPR AWDVVLCISG TLVSCENALV 

        70         80         90        100        110        120 
VAIIVGTPAF RAPMFLLVGS LAVADLLAGL GLVLHFAADF CIGSPEMSLV LVGVLATAFT 

       130        140        150        160        170        180 
ASIGSLLAIT VDRYLSLYNA LTYYSETTVT RTYVMLALVW VGALGLGLVP VLAWNCRDGL 

       190        200        210        220        230        240 
TTCGVVYPLS KNHLVVLAIV FFMVFGIMLQ LYAQICRIVC RHAQQIALQR HLLPASHYVA 

       250        260        270        280        290        300 
TRKGIATLAV VLGAFAACWL PFTVYCLLGD ANSPPLYTYL TLLPATYNSM INPVIYAFRN 

       310        320 
QDVQKVLWAI CCCCSTSKIP FRSRSPSDV 

« Hide

References

« Hide 'large scale' references
[1]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Isolation and chromosomal localization of a novel human G-protein-coupled receptor (GPR3) expressed predominantly in the central nervous system."
Iismaa T.P., Kiefer J., Liu M.L., Baker E., Sutherland G.R., Shine J.
Genomics 24:391-394(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-117.
Strain: New England Deaconess Hospital.
Tissue: Pancreas.
[3]"Sphingosine 1-phosphate is a ligand of the human gpr3, gpr6 and gpr12 family of constitutively active G protein-coupled receptors."
Uhlenbrock K., Gassenhuber J., Kostenis E.
Cell. Signal. 14:941-953(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-329.
[4]"Neural expression of G protein-coupled receptors GPR3, GPR6, and GPR12 up-regulates cyclic AMP levels and promotes neurite outgrowth."
Tanaka S., Ishii K., Kasai K., Yoon S.O., Saeki Y.
J. Biol. Chem. 282:10506-10515(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH473968 Genomic DNA. Translation: EDL80660.1.
L32829 mRNA. Translation: AAA73559.1.
AJ427482 mRNA. Translation: CAD20634.1. Frameshift.
RefSeqNP_714949.1. NM_153727.1.
XP_006239068.1. XM_006239006.1.
XP_006239069.1. XM_006239007.1.
XP_006239070.1. XM_006239008.1.
UniGeneRn.83629.

3D structure databases

ProteinModelPortalQ8K1Q3.
ModBaseSearch...
MobiDBSearch...

Chemistry

GuidetoPHARMACOLOGY83.

Protein family/group databases

GPCRDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID266769.
KEGGrno:266769.
UCSCRGD:628686. rat.

Organism-specific databases

CTD2827.
RGD628686. Gpr3.

Phylogenomic databases

eggNOGNOG320944.
HOGENOMHOG000294075.
KOK04311.
PhylomeDBQ8K1Q3.
TreeFamTF330052.

Gene expression databases

GenevestigatorQ8K1Q3.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000984. GPR3.
IPR000723. GPR_3/6/12_orphan.
[Graphical view]
PANTHERPTHR22750:SF9. PTHR22750:SF9. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00648. GPR3ORPHANR.
PR00644. GPRORPHANR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio624633.
PROQ8K1Q3.

Entry information

Entry nameGPR3_RAT
AccessionPrimary (citable) accession number: Q8K1Q3
Secondary accession number(s): Q63230
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: April 16, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries