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Protein

G-protein coupled receptor 3

Gene

Gpr3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Orphan receptor with constitutive G(s) signaling activity that activate cyclic AMP. Has a potential role in modulating a number of brain functions, including behavioral responses to stress (By similarity), amyloid-beta peptide generation in neurons (By similarity) and neurite outgrowth. Maintains also meiotic arrest in oocytes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor 3
Alternative name(s):
G-protein-coupled receptor R4
Gene namesi
Name:Gpr3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628686. Gpr3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4343ExtracellularSequence AnalysisAdd
BLAST
Transmembranei44 – 6421Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini65 – 739CytoplasmicSequence Analysis
Transmembranei74 – 9421Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini95 – 10814ExtracellularSequence AnalysisAdd
BLAST
Transmembranei109 – 12921Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini130 – 15324CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei154 – 17421Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini175 – 19218ExtracellularSequence AnalysisAdd
BLAST
Transmembranei193 – 21321Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini214 – 24734CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei248 – 26821Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini269 – 2779ExtracellularSequence Analysis
Transmembranei278 – 29821Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini299 – 32931CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cytosol Source: GOC
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329G-protein coupled receptor 3PRO_0000379597Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi20 – 201N-linked (GlcNAc...)Sequence Analysis
Lipidationi312 – 3121S-palmitoyl cysteineBy similarity
Modified residuei323 – 3231PhosphoserineSequence Analysis
Modified residuei325 – 3251PhosphoserineSequence Analysis
Modified residuei327 – 3271PhosphoserineSequence Analysis

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Expressioni

Tissue specificityi

Abundantly expressed in granule neurons at all development stages.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8K1Q3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi311 – 3144Poly-Cys

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG320944.
HOGENOMiHOG000294075.
InParanoidiQ8K1Q3.
KOiK04311.
PhylomeDBiQ8K1Q3.
TreeFamiTF330052.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000984. GPR3.
IPR000723. GPR_3/6/12_orphan.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00648. GPR3ORPHANR.
PR00644. GPRORPHANR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K1Q3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMWGAGRSMA WFSAGSGSVN VSIDPAEEPT GPATLLPSPR AWDVVLCISG
60 70 80 90 100
TLVSCENALV VAIIVGTPAF RAPMFLLVGS LAVADLLAGL GLVLHFAADF
110 120 130 140 150
CIGSPEMSLV LVGVLATAFT ASIGSLLAIT VDRYLSLYNA LTYYSETTVT
160 170 180 190 200
RTYVMLALVW VGALGLGLVP VLAWNCRDGL TTCGVVYPLS KNHLVVLAIV
210 220 230 240 250
FFMVFGIMLQ LYAQICRIVC RHAQQIALQR HLLPASHYVA TRKGIATLAV
260 270 280 290 300
VLGAFAACWL PFTVYCLLGD ANSPPLYTYL TLLPATYNSM INPVIYAFRN
310 320
QDVQKVLWAI CCCCSTSKIP FRSRSPSDV
Length:329
Mass (Da):35,327
Last modified:July 28, 2009 - v2
Checksum:i96E347B2059BD85A
GO

Sequence cautioni

The sequence CAD20634.1 differs from that shown. Reason: Frameshift at positions 210, 224 and 225. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 611V → M in AAA73559 (PubMed:7698767).Curated
Sequence conflicti117 – 1171T → M in AAA73559 (PubMed:7698767).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473968 Genomic DNA. Translation: EDL80660.1.
L32829 mRNA. Translation: AAA73559.1.
AJ427482 mRNA. Translation: CAD20634.1. Frameshift.
RefSeqiNP_714949.1. NM_153727.1.
XP_006239068.1. XM_006239006.2.
UniGeneiRn.83629.

Genome annotation databases

GeneIDi266769.
KEGGirno:266769.
UCSCiRGD:628686. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473968 Genomic DNA. Translation: EDL80660.1.
L32829 mRNA. Translation: AAA73559.1.
AJ427482 mRNA. Translation: CAD20634.1. Frameshift.
RefSeqiNP_714949.1. NM_153727.1.
XP_006239068.1. XM_006239006.2.
UniGeneiRn.83629.

3D structure databases

ProteinModelPortaliQ8K1Q3.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

GuidetoPHARMACOLOGYi83.

Protein family/group databases

GPCRDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi266769.
KEGGirno:266769.
UCSCiRGD:628686. rat.

Organism-specific databases

CTDi2827.
RGDi628686. Gpr3.

Phylogenomic databases

eggNOGiNOG320944.
HOGENOMiHOG000294075.
InParanoidiQ8K1Q3.
KOiK04311.
PhylomeDBiQ8K1Q3.
TreeFamiTF330052.

Miscellaneous databases

NextBioi624633.
PROiQ8K1Q3.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000984. GPR3.
IPR000723. GPR_3/6/12_orphan.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00648. GPR3ORPHANR.
PR00644. GPRORPHANR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Isolation and chromosomal localization of a novel human G-protein-coupled receptor (GPR3) expressed predominantly in the central nervous system."
    Iismaa T.P., Kiefer J., Liu M.L., Baker E., Sutherland G.R., Shine J.
    Genomics 24:391-394(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-117.
    Strain: New England Deaconess Hospital.
    Tissue: Pancreas.
  3. "Sphingosine 1-phosphate is a ligand of the human gpr3, gpr6 and gpr12 family of constitutively active G protein-coupled receptors."
    Uhlenbrock K., Gassenhuber J., Kostenis E.
    Cell. Signal. 14:941-953(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-329.
  4. "Neural expression of G protein-coupled receptors GPR3, GPR6, and GPR12 up-regulates cyclic AMP levels and promotes neurite outgrowth."
    Tanaka S., Ishii K., Kasai K., Yoon S.O., Saeki Y.
    J. Biol. Chem. 282:10506-10515(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiGPR3_RAT
AccessioniPrimary (citable) accession number: Q8K1Q3
Secondary accession number(s): Q63230
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: June 24, 2015
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.