Q8K1Q3 (GPR3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G-protein coupled receptor 3

Alternative name(s):
G-protein-coupled receptor R4

Gene names

Name:

Gpr3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Orphan receptor with constitutive G(s) signaling activity that activate cyclic AMP. Has a potential role in modulating a number of brain functions, including behavioral responses to stress By similarity, amyloid-beta peptide generation in neurons By similarity and neurite outgrowth. Maintains also meiotic arrest in oocytes By similarity. Ref.4
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Abundantly expressed in granule neurons at all development stages. Ref.4
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Sequence caution	The sequence CAD20634.1 differs from that shown. Reason: Frameshift at positions 210, 224 and 225.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	elevation of cytosolic calcium ion concentration Inferred from direct assay Ref.3. Source: RGD positive regulation of cAMP metabolic process Inferred from direct assay Ref.3. Source: RGD
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	G-protein coupled receptor 3		PRO_0000379597
Regions
	Topological domain	1 – 43	43	Extracellular Potential
	Transmembrane	44 – 64	21	Helical; Name=1; Potential
	Topological domain	65 – 73	9	Cytoplasmic Potential
	Transmembrane	74 – 94	21	Helical; Name=2; Potential
	Topological domain	95 – 108	14	Extracellular Potential
	Transmembrane	109 – 129	21	Helical; Name=3; Potential
	Topological domain	130 – 153	24	Cytoplasmic Potential
	Transmembrane	154 – 174	21	Helical; Name=4; Potential
	Topological domain	175 – 192	18	Extracellular Potential
	Transmembrane	193 – 213	21	Helical; Name=5; Potential
	Topological domain	214 – 247	34	Cytoplasmic Potential
	Transmembrane	248 – 268	21	Helical; Name=6; Potential
	Topological domain	269 – 277	9	Extracellular Potential
	Transmembrane	278 – 298	21	Helical; Name=7; Potential
	Topological domain	299 – 329	31	Cytoplasmic Potential
	Compositional bias	311 – 314	4	Poly-Cys
Amino acid modifications
	Modified residue	323	1	Phosphoserine Potential
	Modified residue	325	1	Phosphoserine Potential
	Modified residue	327	1	Phosphoserine Potential
	Lipidation	312	1	S-palmitoyl cysteine By similarity
	Glycosylation	20	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	61	1	V → M in AAA73559. Ref.2
	Sequence conflict	117	1	T → M in AAA73559. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8K1Q3 [UniParc]. Last modified July 28, 2009. Version 2. Checksum: 96E347B2059BD85A	FASTA	329	35,327
	10 20 30 40 50 60 MMWGAGRSMA WFSAGSGSVN VSIDPAEEPT GPATLLPSPR AWDVVLCISG TLVSCENALV 70 80 90 100 110 120 VAIIVGTPAF RAPMFLLVGS LAVADLLAGL GLVLHFAADF CIGSPEMSLV LVGVLATAFT 130 140 150 160 170 180 ASIGSLLAIT VDRYLSLYNA LTYYSETTVT RTYVMLALVW VGALGLGLVP VLAWNCRDGL 190 200 210 220 230 240 TTCGVVYPLS KNHLVVLAIV FFMVFGIMLQ LYAQICRIVC RHAQQIALQR HLLPASHYVA 250 260 270 280 290 300 TRKGIATLAV VLGAFAACWL PFTVYCLLGD ANSPPLYTYL TLLPATYNSM INPVIYAFRN 310 320 QDVQKVLWAI CCCCSTSKIP FRSRSPSDV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Isolation and chromosomal localization of a novel human G-protein-coupled receptor (GPR3) expressed predominantly in the central nervous system." Iismaa T.P., Kiefer J., Liu M.L., Baker E., Sutherland G.R., Shine J. Genomics 24:391-394(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-117. Strain: New England Deaconess Hospital. Tissue: Pancreas.
[3]	"Sphingosine 1-phosphate is a ligand of the human gpr3, gpr6 and gpr12 family of constitutively active G protein-coupled receptors." Uhlenbrock K., Gassenhuber J., Kostenis E. Cell. Signal. 14:941-953(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-329.
[4]	"Neural expression of G protein-coupled receptors GPR3, GPR6, and GPR12 up-regulates cyclic AMP levels and promotes neurite outgrowth." Tanaka S., Ishii K., Kasai K., Yoon S.O., Saeki Y. J. Biol. Chem. 282:10506-10515(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH473968 Genomic DNA. Translation: EDL80660.1. L32829 mRNA. Translation: AAA73559.1. AJ427482 mRNA. Translation: CAD20634.1. Frameshift.
IPI	IPI00200414.
RefSeq	NP_714949.1. NM_153727.1.
UniGene	Rn.83629.
3D structure databases
ProteinModelPortal	Q8K1Q3.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	266769.
KEGG	rno:266769.
UCSC	RGD:628686. rat.
Organism-specific databases
CTD	2827.
RGD	628686. Gpr3.
Phylogenomic databases
eggNOG	NOG320944.
HOGENOM	HOG000294075.
KO	K04311.
OrthoDB	EOG4MSD02.
Gene expression databases
Genevestigator	Q8K1Q3.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR000984. GPR3. IPR000723. GPR_3/6/12_orphan. [Graphical view]
PANTHER	PTHR22750:SF9. PTHR22750:SF9. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00648. GPR3ORPHANR. PR00644. GPRORPHANR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	624633.

Entry information

Entry name

GPR3_RAT

Accession

Primary (citable) accession number: Q8K1Q3
Secondary accession number(s): Q63230

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	July 28, 2009
Last modified:	May 1, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program