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Q8K1P8

- MOT8_RAT

UniProt

Q8K1P8 - MOT8_RAT

Protein

Monocarboxylate transporter 8

Gene

Slc16a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Very active and specific thyroid hormone transporter. Stimulates cellular uptake of thyroxine (T4), triiodothyronine (T3), reverse triiodothyronine (rT3) and diidothyronine. Does not transport Leu, Phe, Trp or Tyr.1 Publication

    Kineticsi

    1. KM=4.0 µM for T3 (at 25 degrees Celsius)
    2. KM=2.2 µM for rT3 (at 25 degrees Celsius)
    3. KM=4.7 µM for T4 (at 25 degrees Celsius)

    GO - Molecular functioni

    1. symporter activity Source: UniProtKB-KW
    2. thyroid hormone transmembrane transporter activity Source: RGD

    GO - Biological processi

    1. hormone transport Source: RGD
    2. thyroid hormone transport Source: GOC

    Keywords - Biological processi

    Symport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Monocarboxylate transporter 8
    Short name:
    MCT 8
    Alternative name(s):
    Solute carrier family 16 member 2
    Gene namesi
    Name:Slc16a2
    Synonyms:Mct8
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi628608. Slc16a2.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 545544Monocarboxylate transporter 8PRO_0000247988Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ8K1P8.

    PTM databases

    PhosphoSiteiQ8K1P8.

    Expressioni

    Tissue specificityi

    Expressed at highest levels in liver, lower levels in brain, kidney and heart (at protein level).1 Publication

    Gene expression databases

    GenevestigatoriQ8K1P8.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000042040.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K1P8.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 102101CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini124 – 14926ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini171 – 18111CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini203 – 2042ExtracellularSequence Analysis
    Topological domaini226 – 23510CytoplasmicSequence Analysis
    Topological domaini257 – 2648ExtracellularSequence Analysis
    Topological domaini286 – 32843CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini350 – 36213ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini384 – 3929CytoplasmicSequence Analysis
    Topological domaini414 – 4152ExtracellularSequence Analysis
    Topological domaini437 – 45317CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini475 – 4839ExtracellularSequence Analysis
    Topological domaini505 – 54541CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei103 – 12321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei150 – 17021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei182 – 20221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei205 – 22521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei236 – 25621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei265 – 28521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei329 – 34921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei363 – 38321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei393 – 41321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei416 – 43621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei454 – 47421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei484 – 50421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati29 – 50221Add
    BLAST
    Repeati51 – 72222Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni29 – 72442 X 22 AA approximate tandem repeatsAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG321880.
    HOGENOMiHOG000046644.
    HOVERGENiHBG006387.
    InParanoidiQ8K1P8.
    KOiK08231.
    PhylomeDBiQ8K1P8.

    Family and domain databases

    InterProiIPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view]
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8K1P8-1 [UniParc]FASTAAdd to Basket

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    MALPSPASEE AEGPCQEANQ EYQEPVCSPV PEPEPEPEPE PEPEPEPVPV    50
    PPPEPQPEPE PQPLPDPAPL PVLGFEAEPV QEPEPTPTVE TRGTARGFQP 100
    PEGGFGWIVV FAATWCNGSI FGIHNSVGIL YSMLLEEEKE KNRQVEFQAA 150
    WVGALAMGMI FFCSPIVSIF TDRLGCRITA TTGAAVAFIG LHTSSFTSPL 200
    SLRYFTYGIL FGCGCSFAFQ PSLVILDHYF QRRLGLANGV VSAGSSIFSM 250
    SFPFLIKMLG DRIKLAQTFQ VLSTFMFVLT LLSLTYRPLL PSSQDTPSKR 300
    GAHTLRQRFL VQFRKYFNMR VFRQRTYRIW AFGIAAAALG YFVPYVHLMK 350
    YVEDKFKEIK ETWVLLVCIG ATSGLGRLVS GHISDSIPGL KKIYLQVLSF 400
    LLLGLMSMMI PLCRDFGGLI VVCLFLGLCD GFFITIMAPI AFELVGPMQA 450
    SQAIGYLLGM MALPMIAGPP IAGLLRNCFG NYHVAFYFAG VPPIIGAVIL 500
    FFVPLMHQRM FKKEQRESSK DKMLSHDPDP NGELLPGSPT PEEPI 545
    Length:545
    Mass (Da):60,118
    Last modified:October 1, 2002 - v1
    Checksum:i74EF7A3DCB9F0DE3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ496570 mRNA. Translation: CAD43059.1.
    RefSeqiNP_671749.1. NM_147216.1.
    UniGeneiRn.208241.

    Genome annotation databases

    GeneIDi259248.
    KEGGirno:259248.
    UCSCiRGD:628608. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ496570 mRNA. Translation: CAD43059.1 .
    RefSeqi NP_671749.1. NM_147216.1.
    UniGenei Rn.208241.

    3D structure databases

    ProteinModelPortali Q8K1P8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000042040.

    Chemistry

    ChEMBLi CHEMBL2073720.

    PTM databases

    PhosphoSitei Q8K1P8.

    Proteomic databases

    PRIDEi Q8K1P8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 259248.
    KEGGi rno:259248.
    UCSCi RGD:628608. rat.

    Organism-specific databases

    CTDi 6567.
    RGDi 628608. Slc16a2.

    Phylogenomic databases

    eggNOGi NOG321880.
    HOGENOMi HOG000046644.
    HOVERGENi HBG006387.
    InParanoidi Q8K1P8.
    KOi K08231.
    PhylomeDBi Q8K1P8.

    Miscellaneous databases

    NextBioi 624302.
    PROi Q8K1P8.

    Gene expression databases

    Genevestigatori Q8K1P8.

    Family and domain databases

    InterProi IPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view ]
    Pfami PF07690. MFS_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of monocarboxylate transporter 8 as a specific thyroid hormone transporter."
      Friesema E.C.H., Ganguly S., Abdalla A., Manning Fox J.E., Halestrap A.P., Visser T.J.
      J. Biol. Chem. 278:40128-40135(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Liver.

    Entry informationi

    Entry nameiMOT8_RAT
    AccessioniPrimary (citable) accession number: Q8K1P8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3