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Protein

Monocarboxylate transporter 8

Gene

SLC16A2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Very active and specific thyroid hormone transporter. Stimulates cellular uptake of thyroxine (T4), triiodothyronine (T3), reverse triiodothyronine (rT3) and diidothyronine. Does not transport Leu, Phe, Trp or Tyr.1 Publication

Kineticsi

  1. KM=4.0 µM for T3 (at 25 degrees Celsius)1 Publication
  2. KM=2.2 µM for rT3 (at 25 degrees Celsius)1 Publication
  3. KM=4.7 µM for T4 (at 25 degrees Celsius)1 Publication

    GO - Molecular functioni

    GO - Biological processi

    • hormone transport Source: RGD
    • ion transmembrane transport Source: GO_Central
    • thyroid hormone transport Source: UniProtKB
    Complete GO annotation...

    Keywords - Biological processi

    Symport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Monocarboxylate transporter 8
    Short name:
    MCT 8
    Alternative name(s):
    Solute carrier family 16 member 2
    Gene namesi
    Name:SLC16A2
    Synonyms:Mct8
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Unplaced

    Organism-specific databases

    RGDi628608. Slc16a2.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini2 – 102CytoplasmicSequence analysisAdd BLAST101
    Transmembranei103 – 123HelicalSequence analysisAdd BLAST21
    Topological domaini124 – 149ExtracellularSequence analysisAdd BLAST26
    Transmembranei150 – 170HelicalSequence analysisAdd BLAST21
    Topological domaini171 – 181CytoplasmicSequence analysisAdd BLAST11
    Transmembranei182 – 202HelicalSequence analysisAdd BLAST21
    Topological domaini203 – 204ExtracellularSequence analysis2
    Transmembranei205 – 225HelicalSequence analysisAdd BLAST21
    Topological domaini226 – 235CytoplasmicSequence analysis10
    Transmembranei236 – 256HelicalSequence analysisAdd BLAST21
    Topological domaini257 – 264ExtracellularSequence analysis8
    Transmembranei265 – 285HelicalSequence analysisAdd BLAST21
    Topological domaini286 – 328CytoplasmicSequence analysisAdd BLAST43
    Transmembranei329 – 349HelicalSequence analysisAdd BLAST21
    Topological domaini350 – 362ExtracellularSequence analysisAdd BLAST13
    Transmembranei363 – 383HelicalSequence analysisAdd BLAST21
    Topological domaini384 – 392CytoplasmicSequence analysis9
    Transmembranei393 – 413HelicalSequence analysisAdd BLAST21
    Topological domaini414 – 415ExtracellularSequence analysis2
    Transmembranei416 – 436HelicalSequence analysisAdd BLAST21
    Topological domaini437 – 453CytoplasmicSequence analysisAdd BLAST17
    Transmembranei454 – 474HelicalSequence analysisAdd BLAST21
    Topological domaini475 – 483ExtracellularSequence analysis9
    Transmembranei484 – 504HelicalSequence analysisAdd BLAST21
    Topological domaini505 – 545CytoplasmicSequence analysisAdd BLAST41

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Chemistry databases

    ChEMBLiCHEMBL2073720.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemovedBy similarity
    ChainiPRO_00002479882 – 545Monocarboxylate transporter 8Add BLAST544

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N-acetylalanineBy similarity1
    Modified residuei540PhosphothreonineBy similarity1

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ8K1P8.
    PRIDEiQ8K1P8.

    PTM databases

    PhosphoSitePlusiQ8K1P8.

    Expressioni

    Tissue specificityi

    Expressed at highest levels in liver, lower levels in brain, kidney and heart (at protein level).1 Publication

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000042040.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K1P8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Repeati29 – 501Add BLAST22
    Repeati51 – 722Add BLAST22

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni29 – 722 X 22 AA approximate tandem repeatsAdd BLAST44

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG2504. Eukaryota.
    COG0477. LUCA.
    HOGENOMiHOG000046644.
    HOVERGENiHBG006387.
    InParanoidiQ8K1P8.
    KOiK08231.
    PhylomeDBiQ8K1P8.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR030761. MCT8.
    IPR011701. MFS.
    IPR020846. MFS_dom.
    [Graphical view]
    PANTHERiPTHR11360:SF123. PTHR11360:SF123. 1 hit.
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8K1P8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MALPSPASEE AEGPCQEANQ EYQEPVCSPV PEPEPEPEPE PEPEPEPVPV
    60 70 80 90 100
    PPPEPQPEPE PQPLPDPAPL PVLGFEAEPV QEPEPTPTVE TRGTARGFQP
    110 120 130 140 150
    PEGGFGWIVV FAATWCNGSI FGIHNSVGIL YSMLLEEEKE KNRQVEFQAA
    160 170 180 190 200
    WVGALAMGMI FFCSPIVSIF TDRLGCRITA TTGAAVAFIG LHTSSFTSPL
    210 220 230 240 250
    SLRYFTYGIL FGCGCSFAFQ PSLVILDHYF QRRLGLANGV VSAGSSIFSM
    260 270 280 290 300
    SFPFLIKMLG DRIKLAQTFQ VLSTFMFVLT LLSLTYRPLL PSSQDTPSKR
    310 320 330 340 350
    GAHTLRQRFL VQFRKYFNMR VFRQRTYRIW AFGIAAAALG YFVPYVHLMK
    360 370 380 390 400
    YVEDKFKEIK ETWVLLVCIG ATSGLGRLVS GHISDSIPGL KKIYLQVLSF
    410 420 430 440 450
    LLLGLMSMMI PLCRDFGGLI VVCLFLGLCD GFFITIMAPI AFELVGPMQA
    460 470 480 490 500
    SQAIGYLLGM MALPMIAGPP IAGLLRNCFG NYHVAFYFAG VPPIIGAVIL
    510 520 530 540
    FFVPLMHQRM FKKEQRESSK DKMLSHDPDP NGELLPGSPT PEEPI
    Length:545
    Mass (Da):60,118
    Last modified:October 1, 2002 - v1
    Checksum:i74EF7A3DCB9F0DE3
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ496570 mRNA. Translation: CAD43059.1.
    RefSeqiNP_671749.1. NM_147216.1.
    UniGeneiRn.208241.

    Genome annotation databases

    GeneIDi259248.
    KEGGirno:259248.
    UCSCiRGD:628608. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ496570 mRNA. Translation: CAD43059.1.
    RefSeqiNP_671749.1. NM_147216.1.
    UniGeneiRn.208241.

    3D structure databases

    ProteinModelPortaliQ8K1P8.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000042040.

    Chemistry databases

    ChEMBLiCHEMBL2073720.

    PTM databases

    PhosphoSitePlusiQ8K1P8.

    Proteomic databases

    PaxDbiQ8K1P8.
    PRIDEiQ8K1P8.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi259248.
    KEGGirno:259248.
    UCSCiRGD:628608. rat.

    Organism-specific databases

    CTDi6567.
    RGDi628608. Slc16a2.

    Phylogenomic databases

    eggNOGiKOG2504. Eukaryota.
    COG0477. LUCA.
    HOGENOMiHOG000046644.
    HOVERGENiHBG006387.
    InParanoidiQ8K1P8.
    KOiK08231.
    PhylomeDBiQ8K1P8.

    Miscellaneous databases

    PROiQ8K1P8.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR030761. MCT8.
    IPR011701. MFS.
    IPR020846. MFS_dom.
    [Graphical view]
    PANTHERiPTHR11360:SF123. PTHR11360:SF123. 1 hit.
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMOT8_RAT
    AccessioniPrimary (citable) accession number: Q8K1P8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: October 1, 2002
    Last modified: November 2, 2016
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.