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Protein

Monocarboxylate transporter 8

Gene

Slc16a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Very active and specific thyroid hormone transporter. Stimulates cellular uptake of thyroxine (T4), triiodothyronine (T3), reverse triiodothyronine (rT3) and diidothyronine. Does not transport Leu, Phe, Trp or Tyr.1 Publication

Kineticsi

  1. KM=4.0 µM for T3 (at 25 degrees Celsius)
  2. KM=2.2 µM for rT3 (at 25 degrees Celsius)
  3. KM=4.7 µM for T4 (at 25 degrees Celsius)

GO - Molecular functioni

  1. symporter activity Source: UniProtKB-KW
  2. thyroid hormone transmembrane transporter activity Source: RGD

GO - Biological processi

  1. hormone transport Source: RGD
  2. ion transmembrane transport Source: GO_Central
  3. thyroid hormone transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Monocarboxylate transporter 8
Short name:
MCT 8
Alternative name(s):
Solute carrier family 16 member 2
Gene namesi
Name:Slc16a2
Synonyms:Mct8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628608. Slc16a2.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 102101CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei103 – 12321HelicalSequence AnalysisAdd
BLAST
Topological domaini124 – 14926ExtracellularSequence AnalysisAdd
BLAST
Transmembranei150 – 17021HelicalSequence AnalysisAdd
BLAST
Topological domaini171 – 18111CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei182 – 20221HelicalSequence AnalysisAdd
BLAST
Topological domaini203 – 2042ExtracellularSequence Analysis
Transmembranei205 – 22521HelicalSequence AnalysisAdd
BLAST
Topological domaini226 – 23510CytoplasmicSequence Analysis
Transmembranei236 – 25621HelicalSequence AnalysisAdd
BLAST
Topological domaini257 – 2648ExtracellularSequence Analysis
Transmembranei265 – 28521HelicalSequence AnalysisAdd
BLAST
Topological domaini286 – 32843CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei329 – 34921HelicalSequence AnalysisAdd
BLAST
Topological domaini350 – 36213ExtracellularSequence AnalysisAdd
BLAST
Transmembranei363 – 38321HelicalSequence AnalysisAdd
BLAST
Topological domaini384 – 3929CytoplasmicSequence Analysis
Transmembranei393 – 41321HelicalSequence AnalysisAdd
BLAST
Topological domaini414 – 4152ExtracellularSequence Analysis
Transmembranei416 – 43621HelicalSequence AnalysisAdd
BLAST
Topological domaini437 – 45317CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei454 – 47421HelicalSequence AnalysisAdd
BLAST
Topological domaini475 – 4839ExtracellularSequence Analysis
Transmembranei484 – 50421HelicalSequence AnalysisAdd
BLAST
Topological domaini505 – 54541CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: GO_Central
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 545544Monocarboxylate transporter 8PRO_0000247988Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ8K1P8.

PTM databases

PhosphoSiteiQ8K1P8.

Expressioni

Tissue specificityi

Expressed at highest levels in liver, lower levels in brain, kidney and heart (at protein level).1 Publication

Gene expression databases

GenevestigatoriQ8K1P8.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000042040.

Structurei

3D structure databases

ProteinModelPortaliQ8K1P8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati29 – 50221Add
BLAST
Repeati51 – 72222Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni29 – 72442 X 22 AA approximate tandem repeatsAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG321880.
HOGENOMiHOG000046644.
HOVERGENiHBG006387.
InParanoidiQ8K1P8.
KOiK08231.
PhylomeDBiQ8K1P8.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K1P8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPSPASEE AEGPCQEANQ EYQEPVCSPV PEPEPEPEPE PEPEPEPVPV
60 70 80 90 100
PPPEPQPEPE PQPLPDPAPL PVLGFEAEPV QEPEPTPTVE TRGTARGFQP
110 120 130 140 150
PEGGFGWIVV FAATWCNGSI FGIHNSVGIL YSMLLEEEKE KNRQVEFQAA
160 170 180 190 200
WVGALAMGMI FFCSPIVSIF TDRLGCRITA TTGAAVAFIG LHTSSFTSPL
210 220 230 240 250
SLRYFTYGIL FGCGCSFAFQ PSLVILDHYF QRRLGLANGV VSAGSSIFSM
260 270 280 290 300
SFPFLIKMLG DRIKLAQTFQ VLSTFMFVLT LLSLTYRPLL PSSQDTPSKR
310 320 330 340 350
GAHTLRQRFL VQFRKYFNMR VFRQRTYRIW AFGIAAAALG YFVPYVHLMK
360 370 380 390 400
YVEDKFKEIK ETWVLLVCIG ATSGLGRLVS GHISDSIPGL KKIYLQVLSF
410 420 430 440 450
LLLGLMSMMI PLCRDFGGLI VVCLFLGLCD GFFITIMAPI AFELVGPMQA
460 470 480 490 500
SQAIGYLLGM MALPMIAGPP IAGLLRNCFG NYHVAFYFAG VPPIIGAVIL
510 520 530 540
FFVPLMHQRM FKKEQRESSK DKMLSHDPDP NGELLPGSPT PEEPI
Length:545
Mass (Da):60,118
Last modified:September 30, 2002 - v1
Checksum:i74EF7A3DCB9F0DE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ496570 mRNA. Translation: CAD43059.1.
RefSeqiNP_671749.1. NM_147216.1.
UniGeneiRn.208241.

Genome annotation databases

GeneIDi259248.
KEGGirno:259248.
UCSCiRGD:628608. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ496570 mRNA. Translation: CAD43059.1.
RefSeqiNP_671749.1. NM_147216.1.
UniGeneiRn.208241.

3D structure databases

ProteinModelPortaliQ8K1P8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000042040.

Chemistry

ChEMBLiCHEMBL2073720.

PTM databases

PhosphoSiteiQ8K1P8.

Proteomic databases

PRIDEiQ8K1P8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi259248.
KEGGirno:259248.
UCSCiRGD:628608. rat.

Organism-specific databases

CTDi6567.
RGDi628608. Slc16a2.

Phylogenomic databases

eggNOGiNOG321880.
HOGENOMiHOG000046644.
HOVERGENiHBG006387.
InParanoidiQ8K1P8.
KOiK08231.
PhylomeDBiQ8K1P8.

Miscellaneous databases

NextBioi624302.
PROiQ8K1P8.

Gene expression databases

GenevestigatoriQ8K1P8.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of monocarboxylate transporter 8 as a specific thyroid hormone transporter."
    Friesema E.C.H., Ganguly S., Abdalla A., Manning Fox J.E., Halestrap A.P., Visser T.J.
    J. Biol. Chem. 278:40128-40135(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Liver.

Entry informationi

Entry nameiMOT8_RAT
AccessioniPrimary (citable) accession number: Q8K1P8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 4, 2006
Last sequence update: September 30, 2002
Last modified: March 3, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.