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Q8K1P8 (MOT8_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Monocarboxylate transporter 8

Short name=MCT 8
Alternative name(s):
Solute carrier family 16 member 2
Gene names
Name:Slc16a2
Synonyms:Mct8
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length545 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Very active and specific thyroid hormone transporter. Stimulates cellular uptake of thyroxine (T4), triiodothyronine (T3), reverse triiodothyronine (rT3) and diidothyronine. Does not transport Leu, Phe, Trp or Tyr. Ref.1

Subunit structure

Homodimer By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.1.

Tissue specificity

Expressed at highest levels in liver, lower levels in brain, kidney and heart (at protein level). Ref.1

Sequence similarities

Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=4.0 µM for T3 (at 25 degrees Celsius)

KM=2.2 µM for rT3 (at 25 degrees Celsius)

KM=4.7 µM for T4 (at 25 degrees Celsius)

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 545544Monocarboxylate transporter 8
PRO_0000247988

Regions

Topological domain2 – 102101Cytoplasmic Potential
Transmembrane103 – 12321Helical; Potential
Topological domain124 – 14926Extracellular Potential
Transmembrane150 – 17021Helical; Potential
Topological domain171 – 18111Cytoplasmic Potential
Transmembrane182 – 20221Helical; Potential
Topological domain203 – 2042Extracellular Potential
Transmembrane205 – 22521Helical; Potential
Topological domain226 – 23510Cytoplasmic Potential
Transmembrane236 – 25621Helical; Potential
Topological domain257 – 2648Extracellular Potential
Transmembrane265 – 28521Helical; Potential
Topological domain286 – 32843Cytoplasmic Potential
Transmembrane329 – 34921Helical; Potential
Topological domain350 – 36213Extracellular Potential
Transmembrane363 – 38321Helical; Potential
Topological domain384 – 3929Cytoplasmic Potential
Transmembrane393 – 41321Helical; Potential
Topological domain414 – 4152Extracellular Potential
Transmembrane416 – 43621Helical; Potential
Topological domain437 – 45317Cytoplasmic Potential
Transmembrane454 – 47421Helical; Potential
Topological domain475 – 4839Extracellular Potential
Transmembrane484 – 50421Helical; Potential
Topological domain505 – 54541Cytoplasmic Potential
Repeat29 – 50221
Repeat51 – 72222
Region29 – 72442 X 22 AA approximate tandem repeats

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K1P8 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 74EF7A3DCB9F0DE3

FASTA54560,118
        10         20         30         40         50         60 
MALPSPASEE AEGPCQEANQ EYQEPVCSPV PEPEPEPEPE PEPEPEPVPV PPPEPQPEPE 

        70         80         90        100        110        120 
PQPLPDPAPL PVLGFEAEPV QEPEPTPTVE TRGTARGFQP PEGGFGWIVV FAATWCNGSI 

       130        140        150        160        170        180 
FGIHNSVGIL YSMLLEEEKE KNRQVEFQAA WVGALAMGMI FFCSPIVSIF TDRLGCRITA 

       190        200        210        220        230        240 
TTGAAVAFIG LHTSSFTSPL SLRYFTYGIL FGCGCSFAFQ PSLVILDHYF QRRLGLANGV 

       250        260        270        280        290        300 
VSAGSSIFSM SFPFLIKMLG DRIKLAQTFQ VLSTFMFVLT LLSLTYRPLL PSSQDTPSKR 

       310        320        330        340        350        360 
GAHTLRQRFL VQFRKYFNMR VFRQRTYRIW AFGIAAAALG YFVPYVHLMK YVEDKFKEIK 

       370        380        390        400        410        420 
ETWVLLVCIG ATSGLGRLVS GHISDSIPGL KKIYLQVLSF LLLGLMSMMI PLCRDFGGLI 

       430        440        450        460        470        480 
VVCLFLGLCD GFFITIMAPI AFELVGPMQA SQAIGYLLGM MALPMIAGPP IAGLLRNCFG 

       490        500        510        520        530        540 
NYHVAFYFAG VPPIIGAVIL FFVPLMHQRM FKKEQRESSK DKMLSHDPDP NGELLPGSPT 


PEEPI 

« Hide

References

[1]"Identification of monocarboxylate transporter 8 as a specific thyroid hormone transporter."
Friesema E.C.H., Ganguly S., Abdalla A., Manning Fox J.E., Halestrap A.P., Visser T.J.
J. Biol. Chem. 278:40128-40135(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ496570 mRNA. Translation: CAD43059.1.
RefSeqNP_671749.1. NM_147216.1.
UniGeneRn.208241.

3D structure databases

ProteinModelPortalQ8K1P8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000042040.

Chemistry

ChEMBLCHEMBL2073720.

PTM databases

PhosphoSiteQ8K1P8.

Proteomic databases

PRIDEQ8K1P8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID259248.
KEGGrno:259248.
UCSCRGD:628608. rat.

Organism-specific databases

CTD6567.
RGD628608. Slc16a2.

Phylogenomic databases

eggNOGNOG321880.
HOGENOMHOG000046644.
HOVERGENHBG006387.
InParanoidQ8K1P8.
KOK08231.
PhylomeDBQ8K1P8.

Gene expression databases

GenevestigatorQ8K1P8.

Family and domain databases

InterProIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio624302.
PROQ8K1P8.

Entry information

Entry nameMOT8_RAT
AccessionPrimary (citable) accession number: Q8K1P8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families