Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription activator BRG1

Gene

Smarca4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1 (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei1505 – 15062Required for binding to 'Lys-15'-acetylated histone 3By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi779 – 7868ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • chromatin binding Source: RGD
  • helicase activity Source: UniProtKB-KW
  • protein complex binding Source: RGD
  • transcription corepressor activity Source: UniProtKB

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: RGD
  • negative regulation of transcription, DNA-templated Source: RGD
  • nervous system development Source: UniProtKB-KW
  • nucleosome disassembly Source: RGD
  • spermatid development Source: RGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Helicase, Hydrolase, Repressor

Keywords - Biological processi

Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription activator BRG1 (EC:3.6.4.-)
Alternative name(s):
ATP-dependent helicase SMARCA4
BRG1-associated factor 190A
Short name:
BAF190A
Protein brahma homolog 1
SNF2-beta
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4
Gene namesi
Name:Smarca4
Synonyms:Baf190a, Brg1, Snf2b, Snf2l4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621728. Smarca4.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • nBAF complex Source: UniProtKB
  • npBAF complex Source: UniProtKB
  • nucleus Source: RGD
  • SWI/SNF complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16131613Transcription activator BRG1PRO_0000391344Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111PhosphothreonineBy similarity
Modified residuei188 – 1881N6-acetyllysineBy similarity
Modified residuei353 – 3531PhosphothreonineBy similarity
Modified residuei609 – 6091PhosphothreonineBy similarity
Modified residuei610 – 6101PhosphoserineBy similarity
Modified residuei613 – 6131PhosphoserineCombined sources
Modified residuei695 – 6951PhosphoserineCombined sources
Modified residuei699 – 6991PhosphoserineCombined sources
Modified residuei1349 – 13491PhosphoserineBy similarity
Modified residuei1390 – 13901PhosphothreonineBy similarity
Modified residuei1419 – 14191PhosphoserineCombined sources
Modified residuei1536 – 15361PhosphoserineCombined sources
Modified residuei1541 – 15411PhosphoserineCombined sources
Modified residuei1552 – 15521PhosphoserineCombined sources
Modified residuei1593 – 15931PhosphoserineCombined sources
Modified residuei1597 – 15971PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8K1P7.
PRIDEiQ8K1P7.

PTM databases

iPTMnetiQ8K1P7.
PhosphoSiteiQ8K1P7.

Interactioni

Subunit structurei

Interacts with NR3C1, PGR, SMARD1, TOPBP1 and ZMIM2/ZIMP7. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1/BAF190A, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, IKFZ1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. Interacts directly with IKFZ1 in the BAF complex. In muscle cells, the BAF complex also contains DPF3. Component of the BAF53 complex, at least composed of BAF53A, RUVBL1, SMARCA4/BRG1/BAF190A, and TRRAP, which preferentially acetylates histone H4 (and H2A) within nucleosomes. Interacts with (via the bromodomain) with TERT; the interaction regulates Wnt-mediated signaling (By similarity). Component of the CREST-BRG1 complex, at least composed of SMARCA4/BRG1/BAF190A, SS18L1/CREST, HDAC1, RB1 and SP1. Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin. Interacts with PHF10/BAF45A. Interacts with ZEB1 (via N-terminus) (By similarity). Interacts with MYOG (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Olig2G3V6122EBI-689301,EBI-6676662
SMARCB1Q128242EBI-689301,EBI-358419From a different organism.
Smarcb1Q4KLI02EBI-689301,EBI-689316

GO - Molecular functioni

  • protein complex binding Source: RGD

Protein-protein interaction databases

IntActiQ8K1P7. 4 interactions.
STRINGi10116.ENSRNOP00000013166.

Structurei

3D structure databases

ProteinModelPortaliQ8K1P7.
SMRiQ8K1P7. Positions 1416-1535.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini171 – 20636QLQPROSITE-ProRule annotationAdd
BLAST
Domaini460 – 53273HSAPROSITE-ProRule annotationAdd
BLAST
Domaini766 – 931166Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini1084 – 1246163Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini1443 – 151371BromoPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 282282Necessary for interaction with SS18L1/CRESTAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi881 – 8844DEGH boxBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi663 – 67210Poly-Glu
Compositional biasi1252 – 1370119Glu-richAdd
BLAST
Compositional biasi1531 – 155525Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 1 bromo domain.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 HSA domain.PROSITE-ProRule annotation
Contains 1 QLQ domain.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain

Phylogenomic databases

eggNOGiKOG0386. Eukaryota.
COG0553. LUCA.
COG5076. LUCA.
HOGENOMiHOG000172363.
HOVERGENiHBG056636.
InParanoidiQ8K1P7.
PhylomeDBiQ8K1P7.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR006576. BRK_domain.
IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR014978. Gln-Leu-Gln_QLQ.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014012. HSA_dom.
IPR027417. P-loop_NTPase.
IPR029295. SnAC.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF07533. BRK. 1 hit.
PF00439. Bromodomain. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07529. HSA. 1 hit.
PF08880. QLQ. 1 hit.
PF14619. SnAC. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00592. BRK. 1 hit.
SM00297. BROMO. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00573. HSA. 1 hit.
SM00951. QLQ. 1 hit.
SM01314. SnAC. 1 hit.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51204. HSA. 1 hit.
PS51666. QLQ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K1P7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPDPPLGG TPRPGPSPGP GPSPGAMLGP SPGPSPGSAH SMMGPSPGPP
60 70 80 90 100
SAGHPMPTQG PGGYPQDNMH QMHKPMESMH EKGMPDDPRY NQMKGMGMRS
110 120 130 140 150
GAHTGMGPPP SPMDQHSQGY PSPLGGSEHA SSPVPASGPS SGPQMSSGPG
160 170 180 190 200
GAPLDGSDPQ ALGQQNRGPT PFNQNQLHQL RAQIMAYKML ARGQPLPDHL
210 220 230 240 250
QMAVQGKRPM PGMQQQMPTL PPPSVSATGP GPGPGPGPGP GPGPAPPNYS
260 270 280 290 300
RPHGMGGPNM PPPGPSGVPP GMPGQPPGGP PKPWPEGPMA NAAAPTSTPQ
310 320 330 340 350
KLIPPQPTGR PSPAPPAVPP AASPVMPPQT QSPGQPAQPA PLVPLHQKQS
360 370 380 390 400
RITPIQKPRG LDPVEILQER EYRLQARIVH RIQELENLPG SLAGDLRTKA
410 420 430 440 450
TIELKALRLL NFQRQLRQEV VVCMRRDTAL ETALNAKAYK RSKRQSLREA
460 470 480 490 500
RITEKLEKQQ KIEQERKRRQ KHQEYLNSIL QHAKDFREYH RSVTGKLQKL
510 520 530 540 550
TKAVATYHAN TEREQKKENE RIEKERMRRL MAEDEEGYRK LIDQKKDKRL
560 570 580 590 600
AYLLQQTDEY VANLTELVRQ HKAAQVAKEK KKKKKKKKAE NAEGQTPAIG
610 620 630 640 650
PDGEPLDETS QMSDLPVKVI HVESGKILTG TDAPKAGQLE AWLEMNPGYE
660 670 680 690 700
VAPRSDSEES GSEEEEEEEE EEQPQPAQPP TLPVEEKKKI PDPDSDDVSE
710 720 730 740 750
VDARHIIENA KQDVDDEYGV SQALARGLQS YYAVAHAVTE RVDKQSALMV
760 770 780 790 800
NGVLKQYQIK GLEWLVSLYN NNLNGILADE MGLGKTIQTI ALITYLMEHK
810 820 830 840 850
RINGPFLIIV PLSTLSNWAY EFDKWAPSVV KVSYKGSPAA RRAFVPQLRS
860 870 880 890 900
GKFNVLLTTY EYIIKDKHIL AKIRWKYMIV DEGHRMKNHH CKLTQVLNTH
910 920 930 940 950
YVAPRRLLLT GTPLQNKLPE LWALLNFLLP TIFKSCSTFE QWFNAPFAMT
960 970 980 990 1000
GEKVDLNEEE TILIIRRLHK VLRPFLLRRL KKEVEAQLPE KVEYVIKCDM
1010 1020 1030 1040 1050
SALQRVLYRH MQAKGVLLTD GSEKDKKGKG GTKTLMNTIM QLRKICNHPY
1060 1070 1080 1090 1100
MFQHIEESFS EHLGFTGGIV QGLDLYRASG KFELLDRILP KLRATNHKVL
1110 1120 1130 1140 1150
LFCQMTSLMT IMEDYFAYRG FKYLRLDGTT KAEDRGMLLK TFNEPGSEYF
1160 1170 1180 1190 1200
IFLLSTRAGG LGLNLQSADT VIIFDSDWNP HQDLQAQDRA HRIGQQNEVR
1210 1220 1230 1240 1250
VLRLCTVNSV EEKILAAAKY KLNVDQKVIQ AGMFDQKSSS HERRAFLQAI
1260 1270 1280 1290 1300
LEHEEQDEEE DEVPDDETVN QMIARHEEEF DLFMRMDLDR RREEARNPKR
1310 1320 1330 1340 1350
KPRLMEEDEL PSWIIKDDAE VERLTCEEEE EKMFGRGSRH RKEVDYSDSL
1360 1370 1380 1390 1400
TEKQWLKAIE EGTLEEIEEE VRQKKSSRKR KRDSEAGSST PTTSTRSRDK
1410 1420 1430 1440 1450
DEESKKQKKR GRPPAEKLSP NPPNLTKKMK KIVDAVIKYK DSSGRQLSEV
1460 1470 1480 1490 1500
FIQLPSRKEL PEYYELIRKP VDFKKIKERI RNHKYRSLND LEKDVMLLCQ
1510 1520 1530 1540 1550
NAQTFNLEGS LIYEDSIVLQ SVFTSVRQKI EKEDDSEGEE SEEEEEGEEE
1560 1570 1580 1590 1600
GSESESRSVK VKIKLGRKEK AQDRLKGGRR RPSRGSRAKP VVSDDDSDEE
1610
QEEDRSGSGS EED
Length:1,613
Mass (Da):181,427
Last modified:October 1, 2002 - v1
Checksum:i7E5F1B04D4C26C87
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ504723 mRNA. Translation: CAD43278.1.
UniGeneiRn.23417.

Genome annotation databases

UCSCiRGD:621728. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ504723 mRNA. Translation: CAD43278.1.
UniGeneiRn.23417.

3D structure databases

ProteinModelPortaliQ8K1P7.
SMRiQ8K1P7. Positions 1416-1535.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8K1P7. 4 interactions.
STRINGi10116.ENSRNOP00000013166.

PTM databases

iPTMnetiQ8K1P7.
PhosphoSiteiQ8K1P7.

Proteomic databases

PaxDbiQ8K1P7.
PRIDEiQ8K1P7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:621728. rat.

Organism-specific databases

RGDi621728. Smarca4.

Phylogenomic databases

eggNOGiKOG0386. Eukaryota.
COG0553. LUCA.
COG5076. LUCA.
HOGENOMiHOG000172363.
HOVERGENiHBG056636.
InParanoidiQ8K1P7.
PhylomeDBiQ8K1P7.

Miscellaneous databases

PROiQ8K1P7.

Family and domain databases

Gene3Di1.20.920.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR006576. BRK_domain.
IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR014978. Gln-Leu-Gln_QLQ.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014012. HSA_dom.
IPR027417. P-loop_NTPase.
IPR029295. SnAC.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF07533. BRK. 1 hit.
PF00439. Bromodomain. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07529. HSA. 1 hit.
PF08880. QLQ. 1 hit.
PF14619. SnAC. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00592. BRK. 1 hit.
SM00297. BROMO. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00573. HSA. 1 hit.
SM00951. QLQ. 1 hit.
SM01314. SnAC. 1 hit.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51204. HSA. 1 hit.
PS51666. QLQ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSMCA4_RAT
AccessioniPrimary (citable) accession number: Q8K1P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: October 1, 2002
Last modified: May 11, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.