Q8K1N2 (PHLB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pleckstrin homology-like domain family B member 2

Alternative name(s):
Protein LL5-beta

Gene names

Name:	Phldb2
Synonyms:	Ll5b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1249 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Seems to be involved in the assembly of the postsynaptic apparatus. May play a role in acetyl-choline receptor (AChR) aggregation in the postsynaptic membrane. Ref.5
Subunit structure	Interacts with FLNC By similarity.
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity. Note: Translocates to the plasma membrane at high levels of PtdIns(3,4,5)P3. At low levels of PtdIns(3,4,5)P3 is translocated to vesicular compartments By similarity.
Tissue specificity	Expressed at postsynaptic membranes of skeletal neuromuscular junctions (at protein level). Ref.5
Developmental stage	In synapses, is expressed at embryonic day 15 and colocalizes with acetyl-choline receptor at prenatal day 4. Expression decreases in adult. Ref.5
Domain	The PH domain mediates the binding to phosphoinositides By similarity.
Sequence similarities	Contains 1 PH domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell intermediate filament cytoskeleton Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8K1N2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8K1N2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     866-873: VSQPQSSE → HRTAVYSGFMSPSTLSPSVTEPSSATWPEVMTTSVDPFPLNDTPPPLPAKKHRRQQPQEQQ

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1249	1249	Pleckstrin homology-like domain family B member 2		PRO_0000053895
Regions
	Domain	1139 – 1242	104	PH
	Coiled coil	580 – 692	113	Potential
	Coiled coil	718 – 803	86	Potential
	Coiled coil	1028 – 1094	67	Potential
	Compositional bias	37 – 416	380	Ser-rich
Amino acid modifications
	Modified residue	71	1	Phosphoserine By similarity
	Modified residue	73	1	Phosphoserine By similarity
	Modified residue	156	1	Phosphoserine By similarity
	Modified residue	333	1	Phosphoserine By similarity
	Modified residue	380	1	Phosphoserine By similarity
	Modified residue	383	1	Phosphoserine By similarity
	Modified residue	389	1	Phosphoserine Ref.6
	Modified residue	411	1	Phosphoserine By similarity
	Modified residue	465	1	Phosphoserine Ref.8 Ref.9
	Modified residue	486	1	Phosphoserine By similarity
	Modified residue	510	1	Phosphoserine Ref.8 Ref.9
	Modified residue	546	1	Phosphothreonine By similarity
	Modified residue	570	1	Phosphothreonine By similarity
	Modified residue	894	1	Phosphothreonine Ref.7
	Modified residue	976	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	866 – 873	8	VSQPQSSE → HRTAVYSGFMSPSTLSPSVT EPSSATWPEVMTTSVDPFPL NDTPPPLPAKKHRRQQPQEQ Q in isoform 2.		VSP_016746
Experimental info
	Sequence conflict	112	1	S → I in AAH50915. Ref.2
	Sequence conflict	112	1	S → I in AAH60683. Ref.2
	Sequence conflict	504	1	R → M in AAM33635. Ref.1
	Sequence conflict	634	1	A → S in BAE38105. Ref.3
	Sequence conflict	807	1	Y → D in AAM33635. Ref.1
	Sequence conflict	1127	1	H → P in AAM33635. Ref.1
	Sequence conflict	1138	1	T → P in AAM33635. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 20, 2005. Version 2. Checksum: 3875492F043ACFAB	FASTA	1,249	141,486
	10 20 30 40 50 60 MAEDSHMQKQ LEFQNGSLEE GFVVRSLENE PQNMMESLSP RKYSSSLKFK ANGDYSGSYL 70 80 90 100 110 120 TLSQPVSAKR SPSPMGTSVR SSPSLAKIQG SKQFCDGIDK NISMKPPISF LSSAASLGGY 130 140 150 160 170 180 PLGKADLDHY TGRDSERSTR LSEKPPYSRY SSRNKSHDSV YFLGGLEGRK TSGSLLTMWN 190 200 210 220 230 240 GNSLSCTGSS PISRSGAASM PSSPKQVRKM NLQDHSTLQP RLSRHKEPAS ENVSVRTRKY 250 260 270 280 290 300 SGSSLSNMGA YSRSLPRLYK ATDNQMSPLS LPPRSSLGNS RRGQLGEKDL PHSLVDSDNY 310 320 330 340 350 360 LNFSSLSSGA SPYKTCLSEG NPYVSSALSV PASPRVARKM LLASTSSDDF DRASYSGTSP 370 380 390 400 410 420 SHSFISGEPD RVLVARRNFS CGSMELDDSD LESLRQSSET PQPVLRERKS SISSISGRDD 430 440 450 460 470 480 LMDYHRRQRE ERLREQEMER LERQRLETIL SLCAEYTKPE GRRLSAGTTV ADVQKINKEL 490 500 510 520 530 540 EKLQLSDEES VFEDALVCPD ARYRCHRKGS LQDVDVAGFG NLGHSASFLA PRGSRSDELL 550 560 570 580 590 600 GDLTRTPPSS SAAFLKATNE SSYLSILPKT PEDIGEEQRT QELAAMEDAR MVILNNLEEL 610 620 630 640 650 660 EQKIKDINDQ MDESSRELDM ECALLDGEQK SETAELMKEK EILDHLNRKI TELEKNIVGE 670 680 690 700 710 720 KTKEKVKLDA EREKLERLQE LYSEQKTQLD NCPESMREQL QQQLKRDADL LDVESKHFED 730 740 750 760 770 780 LEFQQLEHES RLDEEKENLT QQLLREVAEY QRNIVARKEK ISALKKQASH IVQQAQREQD 790 800 810 820 830 840 HFVKEKNNLI MMLQREKENL CNLEKKYSSL TGGKGFPINP NTLKEGYISV NEINESCGNS 850 860 870 880 890 900 TNLSPSTQFP ADADAAVTEP ALAVPVSQPQ SSEHFRSLEE RKKQHKEGLY LSDTLPRKKT 910 920 930 940 950 960 TPSLSPHFSS ATMGRSTTPK AHLPLGQSNS CGSVLPHSLA TMTKDSESRR MLRGYNHQQM 970 980 990 1000 1010 1020 SEGQRQKPEF YSRTASESNV YLNSFHYPDR SYKDQAYDTL SLDSSDSMET SISACSPDNI 1030 1040 1050 1060 1070 1080 SSASTSNIAR IEEMERLLKQ AHAEKTRLLE SREREMEAKK RALEEEKRRR EILEKRLQEE 1090 1100 1110 1120 1130 1140 TSQRQKLIEK EVKIREKQRA QARPLTRYLP VRKEDFDLRS HVETAGHNID TCFHVSITEK 1150 1160 1170 1180 1190 1200 TCRGYLIKMG GKIKTWKKRW FVFDRNKRTF SYYADKHEAK LKGVIYFQAI EEVYYDHLKN 1210 1220 1230 1240 ANKSPNPLLT FSVKTHDRIY YMVAPSPEAM RIWMDVIVTG AEGYTHFLL
	Isoform 2 [UniParc]. Checksum: 7CC09EC3F4379D09	FASTA	1,302	147,399
	10 20 30 40 50 60 MAEDSHMQKQ LEFQNGSLEE GFVVRSLENE PQNMMESLSP RKYSSSLKFK ANGDYSGSYL 70 80 90 100 110 120 TLSQPVSAKR SPSPMGTSVR SSPSLAKIQG SKQFCDGIDK NISMKPPISF LSSAASLGGY 130 140 150 160 170 180 PLGKADLDHY TGRDSERSTR LSEKPPYSRY SSRNKSHDSV YFLGGLEGRK TSGSLLTMWN 190 200 210 220 230 240 GNSLSCTGSS PISRSGAASM PSSPKQVRKM NLQDHSTLQP RLSRHKEPAS ENVSVRTRKY 250 260 270 280 290 300 SGSSLSNMGA YSRSLPRLYK ATDNQMSPLS LPPRSSLGNS RRGQLGEKDL PHSLVDSDNY 310 320 330 340 350 360 LNFSSLSSGA SPYKTCLSEG NPYVSSALSV PASPRVARKM LLASTSSDDF DRASYSGTSP 370 380 390 400 410 420 SHSFISGEPD RVLVARRNFS CGSMELDDSD LESLRQSSET PQPVLRERKS SISSISGRDD 430 440 450 460 470 480 LMDYHRRQRE ERLREQEMER LERQRLETIL SLCAEYTKPE GRRLSAGTTV ADVQKINKEL 490 500 510 520 530 540 EKLQLSDEES VFEDALVCPD ARYRCHRKGS LQDVDVAGFG NLGHSASFLA PRGSRSDELL 550 560 570 580 590 600 GDLTRTPPSS SAAFLKATNE SSYLSILPKT PEDIGEEQRT QELAAMEDAR MVILNNLEEL 610 620 630 640 650 660 EQKIKDINDQ MDESSRELDM ECALLDGEQK SETAELMKEK EILDHLNRKI TELEKNIVGE 670 680 690 700 710 720 KTKEKVKLDA EREKLERLQE LYSEQKTQLD NCPESMREQL QQQLKRDADL LDVESKHFED 730 740 750 760 770 780 LEFQQLEHES RLDEEKENLT QQLLREVAEY QRNIVARKEK ISALKKQASH IVQQAQREQD 790 800 810 820 830 840 HFVKEKNNLI MMLQREKENL CNLEKKYSSL TGGKGFPINP NTLKEGYISV NEINESCGNS 850 860 870 880 890 900 TNLSPSTQFP ADADAAVTEP ALAVPHRTAV YSGFMSPSTL SPSVTEPSSA TWPEVMTTSV 910 920 930 940 950 960 DPFPLNDTPP PLPAKKHRRQ QPQEQQHFRS LEERKKQHKE GLYLSDTLPR KKTTPSLSPH 970 980 990 1000 1010 1020 FSSATMGRST TPKAHLPLGQ SNSCGSVLPH SLATMTKDSE SRRMLRGYNH QQMSEGQRQK 1030 1040 1050 1060 1070 1080 PEFYSRTASE SNVYLNSFHY PDRSYKDQAY DTLSLDSSDS METSISACSP DNISSASTSN 1090 1100 1110 1120 1130 1140 IARIEEMERL LKQAHAEKTR LLESREREME AKKRALEEEK RRREILEKRL QEETSQRQKL 1150 1160 1170 1180 1190 1200 IEKEVKIREK QRAQARPLTR YLPVRKEDFD LRSHVETAGH NIDTCFHVSI TEKTCRGYLI 1210 1220 1230 1240 1250 1260 KMGGKIKTWK KRWFVFDRNK RTFSYYADKH EAKLKGVIYF QAIEEVYYDH LKNANKSPNP 1270 1280 1290 1300 LLTFSVKTHD RIYYMVAPSP EAMRIWMDVI VTGAEGYTHF LL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Guo J.H., Yu L. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: BALB/c.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-793 (ISOFORM 1). Strain: C57BL/6J. Tissue: Spleen.
[4]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 570-579 AND 698-705, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[5]	"LL5beta: a regulator of postsynaptic differentiation identified in a screen for synaptically enriched transcripts at the neuromuscular junction." Kishi M., Kummer T.T., Eglen S.J., Sanes J.R. J. Cell Biol. 169:355-366(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[6]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-389, MASS SPECTROMETRY. Tissue: Liver.
[7]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-894 AND SER-976, MASS SPECTROMETRY. Tissue: Liver.
[8]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-465 AND SER-510, MASS SPECTROMETRY. Tissue: Melanoma.
[9]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-465 AND SER-510, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF506821 mRNA. Translation: AAM33635.1. BC050915 mRNA. Translation: AAH50915.1. BC060683 mRNA. Translation: AAH60683.1. AK049595 mRNA. Translation: BAC33833.1. AK165251 mRNA. Translation: BAE38105.1.
IPI	IPI00330773. IPI00403827.
RefSeq	NP_001239371.1. NM_001252442.1. NP_700461.2. NM_153412.4.
UniGene	Mm.211477.
3D structure databases
ProteinModelPortal	Q8K1N2.
SMR	Q8K1N2. Positions 1144-1237.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8K1N2. 1 interaction.
STRING	10090.ENSMUSP00000075672.
PTM databases
PhosphoSite	Q8K1N2.
Proteomic databases
PaxDb	Q8K1N2.
PRIDE	Q8K1N2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000036355; ENSMUSP00000046496; ENSMUSG00000033149. ENSMUST00000076333; ENSMUSP00000075672; ENSMUSG00000033149.
GeneID	208177.
KEGG	mmu:208177.
UCSC	uc007zjb.2. mouse. uc012agj.1. mouse.
Organism-specific databases
CTD	90102.
MGI	MGI:2444981. Phldb2.
Phylogenomic databases
eggNOG	NOG86245.
GeneTree	ENSGT00530000063148.
HOGENOM	HOG000286004.
HOVERGEN	HBG082124.
OMA	LNSFHYP.
OrthoDB	EOG4FJ881.
Gene expression databases
ArrayExpress	Q8K1N2.
Bgee	Q8K1N2.
Genevestigator	Q8K1N2.
GermOnline	ENSMUSG00000033149. Mus musculus.
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. [Graphical view]
Pfam	PF00169. PH. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	372202.
SOURCE	Search...

Entry information

Entry name

PHLB2_MOUSE

Accession

Primary (citable) accession number: Q8K1N2
Secondary accession number(s): Q3TNI3, Q80Y16, Q8BKV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program