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Q8K1N1

- PLPL8_MOUSE

UniProt

Q8K1N1 - PLPL8_MOUSE

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Protein

Calcium-independent phospholipase A2-gamma

Gene

Pnpla8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Calcium-independent phospholipase A2, which catalyzes the hydrolysis of the sn-2 position of glycerophospholipids, PtdSer and to a lower extent PtdCho. Cleaves membrane phospholipids (By similarity).By similarity

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by E-6-bromomethylene-3-1-naphthalenyl-2H-tetrahydropyran-2-one (BEL).By similarity

GO - Molecular functioni

  1. calcium-independent phospholipase A2 activity Source: UniProtKB
  2. lysophospholipase activity Source: UniProtKB-EC

GO - Biological processi

  1. arachidonic acid metabolic process Source: Ensembl
  2. arachidonic acid secretion Source: Ensembl
  3. cell death Source: Ensembl
  4. fatty acid metabolic process Source: UniProtKB
  5. linoleic acid metabolic process Source: Ensembl
  6. phosphatidylcholine catabolic process Source: Ensembl
  7. phosphatidylethanolamine catabolic process Source: Ensembl
  8. prostaglandin biosynthetic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

ReactomeiREACT_199027. Acyl chain remodelling of PE.
REACT_199038. Acyl chain remodelling of PC.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-independent phospholipase A2-gamma (EC:3.1.1.5)
Alternative name(s):
Intracellular membrane-associated calcium-independent phospholipase A2 gamma
Short name:
iPLA2-gamma
Patatin-like phospholipase domain-containing protein 8
Gene namesi
Name:Pnpla8
Synonyms:Ipla22, Ipla2g
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:1914702. Pnpla8.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. Golgi apparatus Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
  4. intracellular Source: UniProtKB
  5. peroxisomal membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 776776Calcium-independent phospholipase A2-gammaPRO_0000303215Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi4 – 41N-linked (GlcNAc...)Sequence Analysis
Glycosylationi157 – 1571N-linked (GlcNAc...)Sequence Analysis
Modified residuei730 – 7301N6-succinyllysine1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8K1N1.
PaxDbiQ8K1N1.
PRIDEiQ8K1N1.

PTM databases

PhosphoSiteiQ8K1N1.

Expressioni

Gene expression databases

BgeeiQ8K1N1.
CleanExiMM_PNPLA8.
ExpressionAtlasiQ8K1N1. baseline and differential.
GenevestigatoriQ8K1N1.

Structurei

3D structure databases

ProteinModelPortaliQ8K1N1.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei469 – 48921HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini439 – 634196PatatinAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi475 – 4795GXSXGBy similarity

Sequence similaritiesi

Contains 1 patatin domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3621.
GeneTreeiENSGT00530000063645.
HOGENOMiHOG000115579.
HOVERGENiHBG102100.
InParanoidiQ8K1N1.
KOiK16815.
OMAiKYDSKSQ.
PhylomeDBiQ8K1N1.
TreeFamiTF319230.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002641. Patatin/PLipase_A2-rel.
[Graphical view]
PfamiPF01734. Patatin. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8K1N1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSINLTLDIY IYFLNNARSL CGKQRSKQLH FVCSKQYWRM NHVNVHREFH
60 70 80 90 100
TSKKSCKWNR SEAHCSKHWH SPSNHGLHFG IVRLSTSAPK GLTKVSIHMS
110 120 130 140 150
RIKSTLNSVS KAIFGSQNEM VTRLAQFKPS SRILRKVSDK GWLKQKNVKQ
160 170 180 190 200
AVESLKNYSD KSAGKNSLAE QKSYFADKEE DSGKHSLFHY TYGITTRFGE
210 220 230 240 250
SFSVLANHIN SYFKSKGKMS QTKEDKQLQD KPDLEERKSS SPGPDTVADR
260 270 280 290 300
PDSESPLEVK DKLSSPTQMP EAHPVSAKQS IANFLSRPTE GVQALVGGYI
310 320 330 340 350
GGLVPKLKSD PKSPPEEQEV SAKTEQAVNK DKKAEEKKRV LLQQEKIIAR
360 370 380 390 400
VSIDNRTRAL VQALRRTADP KLCITRVEEL TFHLLEFPEG KGVAIKEKII
410 420 430 440 450
PYLLRLRQVK DETLQAAVRE ILALIGYVDP VKGRGIRILT IDGGGTRGVV
460 470 480 490 500
ALQTLRKLVE LTQKPIHQLF DYICGVSTGA ILAFMLGLFH MPLDECEELY
510 520 530 540 550
RKLGSDVFTQ NVIVGTVKMS WSHAFYDSNT WEKILKDRIG SALMIETARN
560 570 580 590 600
PACPKVAAIS TIVNRGQTPK AFVFRNYGHF PGTNSHYLGG CQYKMWQAIR
610 620 630 640 650
ASSAAPGYFA EYALGSDLHQ DGGLLLNNPS ALALHECKCI WPDTPLECIV
660 670 680 690 700
SLGTGRYESD VRNTSTYTSL KTKLSNVISS ATDTEEVHIM LDGLLPSDTY
710 720 730 740 750
FRFNPVICEN IPLDESRDEK LDQLQLEGMK YIERNDQKMK KVAKILSQEK
760 770
TTLQKINDWI KLKSDMYEGL PFFSKL
Length:776
Mass (Da):87,381
Last modified:October 1, 2002 - v1
Checksum:i1140CC8358B1E119
GO

Sequence cautioni

The sequence AAH19364.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAB23417.1 differs from that shown. Reason: Erroneous termination at position 777. Translated as stop.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti438 – 4414ILTI → DAWV in AAH19364. (PubMed:15489334)Curated
Sequence conflicti729 – 7291M → I in BAE40365. (PubMed:16141072)Curated
Sequence conflicti749 – 7491E → Q in BAE40365. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB044139 mRNA. Translation: BAB97200.1.
AK004621 mRNA. Translation: BAB23417.1. Sequence problems.
AK145776 mRNA. Translation: BAE26645.1.
AK163211 mRNA. Translation: BAE37236.1.
AK168475 mRNA. Translation: BAE40365.1.
BC019364 mRNA. Translation: AAH19364.1. Different initiation.
BC127056 mRNA. Translation: AAI27057.1.
CCDSiCCDS36436.1.
RefSeqiNP_080440.2. NM_026164.2.
UniGeneiMm.54126.

Genome annotation databases

EnsembliENSMUST00000043082; ENSMUSP00000043286; ENSMUSG00000036257.
GeneIDi67452.
KEGGimmu:67452.
UCSCiuc007nlp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB044139 mRNA. Translation: BAB97200.1 .
AK004621 mRNA. Translation: BAB23417.1 . Sequence problems.
AK145776 mRNA. Translation: BAE26645.1 .
AK163211 mRNA. Translation: BAE37236.1 .
AK168475 mRNA. Translation: BAE40365.1 .
BC019364 mRNA. Translation: AAH19364.1 . Different initiation.
BC127056 mRNA. Translation: AAI27057.1 .
CCDSi CCDS36436.1.
RefSeqi NP_080440.2. NM_026164.2.
UniGenei Mm.54126.

3D structure databases

ProteinModelPortali Q8K1N1.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q8K1N1.

Proteomic databases

MaxQBi Q8K1N1.
PaxDbi Q8K1N1.
PRIDEi Q8K1N1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000043082 ; ENSMUSP00000043286 ; ENSMUSG00000036257 .
GeneIDi 67452.
KEGGi mmu:67452.
UCSCi uc007nlp.1. mouse.

Organism-specific databases

CTDi 50640.
MGIi MGI:1914702. Pnpla8.

Phylogenomic databases

eggNOGi COG3621.
GeneTreei ENSGT00530000063645.
HOGENOMi HOG000115579.
HOVERGENi HBG102100.
InParanoidi Q8K1N1.
KOi K16815.
OMAi KYDSKSQ.
PhylomeDBi Q8K1N1.
TreeFami TF319230.

Enzyme and pathway databases

Reactomei REACT_199027. Acyl chain remodelling of PE.
REACT_199038. Acyl chain remodelling of PC.

Miscellaneous databases

NextBioi 324606.
PROi Q8K1N1.
SOURCEi Search...

Gene expression databases

Bgeei Q8K1N1.
CleanExi MM_PNPLA8.
ExpressionAtlasi Q8K1N1. baseline and differential.
Genevestigatori Q8K1N1.

Family and domain databases

InterProi IPR016035. Acyl_Trfase/lysoPLipase.
IPR002641. Patatin/PLipase_A2-rel.
[Graphical view ]
Pfami PF01734. Patatin. 1 hit.
[Graphical view ]
SUPFAMi SSF52151. SSF52151. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel intracellular membrane-bound calcium-independent phospholipase A(2)."
    Tanaka H., Takeya R., Sumimoto H.
    Biochem. Biophys. Res. Commun. 272:320-326(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Egg, Kidney and Lung.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary tumor.
  4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-730, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiPLPL8_MOUSE
AccessioniPrimary (citable) accession number: Q8K1N1
Secondary accession number(s): Q3TH33, Q8VEC0, Q9DC20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3