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Protein

Calcium-independent phospholipase A2-gamma

Gene

Pnpla8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-independent phospholipase A2, which catalyzes the hydrolysis of the sn-2 position of glycerophospholipids, PtdSer and to a lower extent PtdCho. Cleaves membrane phospholipids (By similarity).By similarity

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by E-6-bromomethylene-3-1-naphthalenyl-2H-tetrahydropyran-2-one (BEL).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei477NucleophilePROSITE-ProRule annotation1
Active sitei621Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-1482788. Acyl chain remodelling of PC.
R-MMU-1482839. Acyl chain remodelling of PE.

Chemistry databases

SwissLipidsiSLP:000000594.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-independent phospholipase A2-gamma (EC:3.1.1.5)
Alternative name(s):
Intracellular membrane-associated calcium-independent phospholipase A2 gamma
Short name:
iPLA2-gamma
Patatin-like phospholipase domain-containing protein 8
Gene namesi
Name:Pnpla8
Synonyms:Ipla22, Ipla2g
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1914702. Pnpla8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei469 – 489HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3259504.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003032151 – 776Calcium-independent phospholipase A2-gammaAdd BLAST776

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi4N-linked (GlcNAc...)Sequence analysis1
Glycosylationi157N-linked (GlcNAc...)Sequence analysis1
Modified residuei730N6-succinyllysineCombined sources1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8K1N1.
PaxDbiQ8K1N1.
PeptideAtlasiQ8K1N1.
PRIDEiQ8K1N1.

PTM databases

iPTMnetiQ8K1N1.
PhosphoSitePlusiQ8K1N1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000036257.
CleanExiMM_PNPLA8.
ExpressionAtlasiQ8K1N1. baseline and differential.
GenevisibleiQ8K1N1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043286.

Structurei

3D structure databases

ProteinModelPortaliQ8K1N1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini439 – 634PNPLAPROSITE-ProRule annotationAdd BLAST196

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi443 – 448GXGXXGPROSITE-ProRule annotation6
Motifi475 – 479GXSXGPROSITE-ProRule annotation5
Motifi621 – 623DGA/GPROSITE-ProRule annotation3

Sequence similaritiesi

Contains 1 PNPLA (patatin-like phospholipase) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4231. Eukaryota.
ENOG410XQXJ. LUCA.
GeneTreeiENSGT00530000063645.
HOGENOMiHOG000115579.
HOVERGENiHBG102100.
InParanoidiQ8K1N1.
KOiK16815.
OMAiKYDSKSQ.
OrthoDBiEOG091G05AJ.
PhylomeDBiQ8K1N1.
TreeFamiTF319230.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002641. Patatin/PLipase_A2-rel.
[Graphical view]
PfamiPF01734. Patatin. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.
PROSITEiPS51635. PNPLA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K1N1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSINLTLDIY IYFLNNARSL CGKQRSKQLH FVCSKQYWRM NHVNVHREFH
60 70 80 90 100
TSKKSCKWNR SEAHCSKHWH SPSNHGLHFG IVRLSTSAPK GLTKVSIHMS
110 120 130 140 150
RIKSTLNSVS KAIFGSQNEM VTRLAQFKPS SRILRKVSDK GWLKQKNVKQ
160 170 180 190 200
AVESLKNYSD KSAGKNSLAE QKSYFADKEE DSGKHSLFHY TYGITTRFGE
210 220 230 240 250
SFSVLANHIN SYFKSKGKMS QTKEDKQLQD KPDLEERKSS SPGPDTVADR
260 270 280 290 300
PDSESPLEVK DKLSSPTQMP EAHPVSAKQS IANFLSRPTE GVQALVGGYI
310 320 330 340 350
GGLVPKLKSD PKSPPEEQEV SAKTEQAVNK DKKAEEKKRV LLQQEKIIAR
360 370 380 390 400
VSIDNRTRAL VQALRRTADP KLCITRVEEL TFHLLEFPEG KGVAIKEKII
410 420 430 440 450
PYLLRLRQVK DETLQAAVRE ILALIGYVDP VKGRGIRILT IDGGGTRGVV
460 470 480 490 500
ALQTLRKLVE LTQKPIHQLF DYICGVSTGA ILAFMLGLFH MPLDECEELY
510 520 530 540 550
RKLGSDVFTQ NVIVGTVKMS WSHAFYDSNT WEKILKDRIG SALMIETARN
560 570 580 590 600
PACPKVAAIS TIVNRGQTPK AFVFRNYGHF PGTNSHYLGG CQYKMWQAIR
610 620 630 640 650
ASSAAPGYFA EYALGSDLHQ DGGLLLNNPS ALALHECKCI WPDTPLECIV
660 670 680 690 700
SLGTGRYESD VRNTSTYTSL KTKLSNVISS ATDTEEVHIM LDGLLPSDTY
710 720 730 740 750
FRFNPVICEN IPLDESRDEK LDQLQLEGMK YIERNDQKMK KVAKILSQEK
760 770
TTLQKINDWI KLKSDMYEGL PFFSKL
Length:776
Mass (Da):87,381
Last modified:October 1, 2002 - v1
Checksum:i1140CC8358B1E119
GO

Sequence cautioni

The sequence AAH19364 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB23417 differs from that shown. Reason: Erroneous termination at position 777. Translated as stop.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti438 – 441ILTI → DAWV in AAH19364 (PubMed:15489334).Curated4
Sequence conflicti729M → I in BAE40365 (PubMed:16141072).Curated1
Sequence conflicti749E → Q in BAE40365 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB044139 mRNA. Translation: BAB97200.1.
AK004621 mRNA. Translation: BAB23417.1. Sequence problems.
AK145776 mRNA. Translation: BAE26645.1.
AK163211 mRNA. Translation: BAE37236.1.
AK168475 mRNA. Translation: BAE40365.1.
BC019364 mRNA. Translation: AAH19364.1. Different initiation.
BC127056 mRNA. Translation: AAI27057.1.
CCDSiCCDS36436.1.
RefSeqiNP_080440.2. NM_026164.2.
UniGeneiMm.54126.

Genome annotation databases

EnsembliENSMUST00000043082; ENSMUSP00000043286; ENSMUSG00000036257.
GeneIDi67452.
KEGGimmu:67452.
UCSCiuc007nlp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB044139 mRNA. Translation: BAB97200.1.
AK004621 mRNA. Translation: BAB23417.1. Sequence problems.
AK145776 mRNA. Translation: BAE26645.1.
AK163211 mRNA. Translation: BAE37236.1.
AK168475 mRNA. Translation: BAE40365.1.
BC019364 mRNA. Translation: AAH19364.1. Different initiation.
BC127056 mRNA. Translation: AAI27057.1.
CCDSiCCDS36436.1.
RefSeqiNP_080440.2. NM_026164.2.
UniGeneiMm.54126.

3D structure databases

ProteinModelPortaliQ8K1N1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043286.

Chemistry databases

ChEMBLiCHEMBL3259504.
SwissLipidsiSLP:000000594.

PTM databases

iPTMnetiQ8K1N1.
PhosphoSitePlusiQ8K1N1.

Proteomic databases

MaxQBiQ8K1N1.
PaxDbiQ8K1N1.
PeptideAtlasiQ8K1N1.
PRIDEiQ8K1N1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043082; ENSMUSP00000043286; ENSMUSG00000036257.
GeneIDi67452.
KEGGimmu:67452.
UCSCiuc007nlp.1. mouse.

Organism-specific databases

CTDi50640.
MGIiMGI:1914702. Pnpla8.

Phylogenomic databases

eggNOGiKOG4231. Eukaryota.
ENOG410XQXJ. LUCA.
GeneTreeiENSGT00530000063645.
HOGENOMiHOG000115579.
HOVERGENiHBG102100.
InParanoidiQ8K1N1.
KOiK16815.
OMAiKYDSKSQ.
OrthoDBiEOG091G05AJ.
PhylomeDBiQ8K1N1.
TreeFamiTF319230.

Enzyme and pathway databases

ReactomeiR-MMU-1482788. Acyl chain remodelling of PC.
R-MMU-1482839. Acyl chain remodelling of PE.

Miscellaneous databases

PROiQ8K1N1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036257.
CleanExiMM_PNPLA8.
ExpressionAtlasiQ8K1N1. baseline and differential.
GenevisibleiQ8K1N1. MM.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002641. Patatin/PLipase_A2-rel.
[Graphical view]
PfamiPF01734. Patatin. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.
PROSITEiPS51635. PNPLA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLPL8_MOUSE
AccessioniPrimary (citable) accession number: Q8K1N1
Secondary accession number(s): Q3TH33, Q8VEC0, Q9DC20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.