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Q8K1N1

- PLPL8_MOUSE

UniProt

Q8K1N1 - PLPL8_MOUSE

Protein

Calcium-independent phospholipase A2-gamma

Gene

Pnpla8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Calcium-independent phospholipase A2, which catalyzes the hydrolysis of the sn-2 position of glycerophospholipids, PtdSer and to a lower extent PtdCho. Cleaves membrane phospholipids By similarity.By similarity

    Catalytic activityi

    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

    Enzyme regulationi

    Inhibited by E-6-bromomethylene-3-1-naphthalenyl-2H-tetrahydropyran-2-one (BEL).By similarity

    GO - Molecular functioni

    1. calcium-independent phospholipase A2 activity Source: UniProtKB
    2. lysophospholipase activity Source: UniProtKB-EC

    GO - Biological processi

    1. arachidonic acid metabolic process Source: Ensembl
    2. arachidonic acid secretion Source: Ensembl
    3. cell death Source: Ensembl
    4. fatty acid metabolic process Source: UniProtKB
    5. linoleic acid metabolic process Source: Ensembl
    6. phosphatidylcholine catabolic process Source: Ensembl
    7. phosphatidylethanolamine catabolic process Source: Ensembl
    8. prostaglandin biosynthetic process Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    ReactomeiREACT_199027. Acyl chain remodelling of PE.
    REACT_199038. Acyl chain remodelling of PC.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium-independent phospholipase A2-gamma (EC:3.1.1.5)
    Alternative name(s):
    Intracellular membrane-associated calcium-independent phospholipase A2 gamma
    Short name:
    iPLA2-gamma
    Patatin-like phospholipase domain-containing protein 8
    Gene namesi
    Name:Pnpla8
    Synonyms:Ipla22, Ipla2g
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:1914702. Pnpla8.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. Golgi membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. intracellular Source: UniProtKB
    5. perinuclear region of cytoplasm Source: UniProtKB-SubCell
    6. peroxisomal membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 776776Calcium-independent phospholipase A2-gammaPRO_0000303215Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi4 – 41N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi157 – 1571N-linked (GlcNAc...)Sequence Analysis
    Modified residuei730 – 7301N6-succinyllysine1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ8K1N1.
    PaxDbiQ8K1N1.
    PRIDEiQ8K1N1.

    PTM databases

    PhosphoSiteiQ8K1N1.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8K1N1.
    BgeeiQ8K1N1.
    CleanExiMM_PNPLA8.
    GenevestigatoriQ8K1N1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K1N1.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei469 – 48921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini439 – 634196PatatinAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi475 – 4795GXSXGBy similarity

    Sequence similaritiesi

    Contains 1 patatin domain.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG3621.
    GeneTreeiENSGT00530000063645.
    HOGENOMiHOG000115579.
    HOVERGENiHBG102100.
    InParanoidiQ8K1N1.
    KOiK16815.
    OMAiKYDSKSQ.
    PhylomeDBiQ8K1N1.
    TreeFamiTF319230.

    Family and domain databases

    InterProiIPR016035. Acyl_Trfase/lysoPLipase.
    IPR002641. Patatin/PLipase_A2-rel.
    [Graphical view]
    PfamiPF01734. Patatin. 1 hit.
    [Graphical view]
    SUPFAMiSSF52151. SSF52151. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8K1N1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSINLTLDIY IYFLNNARSL CGKQRSKQLH FVCSKQYWRM NHVNVHREFH    50
    TSKKSCKWNR SEAHCSKHWH SPSNHGLHFG IVRLSTSAPK GLTKVSIHMS 100
    RIKSTLNSVS KAIFGSQNEM VTRLAQFKPS SRILRKVSDK GWLKQKNVKQ 150
    AVESLKNYSD KSAGKNSLAE QKSYFADKEE DSGKHSLFHY TYGITTRFGE 200
    SFSVLANHIN SYFKSKGKMS QTKEDKQLQD KPDLEERKSS SPGPDTVADR 250
    PDSESPLEVK DKLSSPTQMP EAHPVSAKQS IANFLSRPTE GVQALVGGYI 300
    GGLVPKLKSD PKSPPEEQEV SAKTEQAVNK DKKAEEKKRV LLQQEKIIAR 350
    VSIDNRTRAL VQALRRTADP KLCITRVEEL TFHLLEFPEG KGVAIKEKII 400
    PYLLRLRQVK DETLQAAVRE ILALIGYVDP VKGRGIRILT IDGGGTRGVV 450
    ALQTLRKLVE LTQKPIHQLF DYICGVSTGA ILAFMLGLFH MPLDECEELY 500
    RKLGSDVFTQ NVIVGTVKMS WSHAFYDSNT WEKILKDRIG SALMIETARN 550
    PACPKVAAIS TIVNRGQTPK AFVFRNYGHF PGTNSHYLGG CQYKMWQAIR 600
    ASSAAPGYFA EYALGSDLHQ DGGLLLNNPS ALALHECKCI WPDTPLECIV 650
    SLGTGRYESD VRNTSTYTSL KTKLSNVISS ATDTEEVHIM LDGLLPSDTY 700
    FRFNPVICEN IPLDESRDEK LDQLQLEGMK YIERNDQKMK KVAKILSQEK 750
    TTLQKINDWI KLKSDMYEGL PFFSKL 776
    Length:776
    Mass (Da):87,381
    Last modified:October 1, 2002 - v1
    Checksum:i1140CC8358B1E119
    GO

    Sequence cautioni

    The sequence AAH19364.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAB23417.1 differs from that shown. Reason: Erroneous termination at position 777. Translated as stop.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti438 – 4414ILTI → DAWV in AAH19364. (PubMed:15489334)Curated
    Sequence conflicti729 – 7291M → I in BAE40365. (PubMed:16141072)Curated
    Sequence conflicti749 – 7491E → Q in BAE40365. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB044139 mRNA. Translation: BAB97200.1.
    AK004621 mRNA. Translation: BAB23417.1. Sequence problems.
    AK145776 mRNA. Translation: BAE26645.1.
    AK163211 mRNA. Translation: BAE37236.1.
    AK168475 mRNA. Translation: BAE40365.1.
    BC019364 mRNA. Translation: AAH19364.1. Different initiation.
    BC127056 mRNA. Translation: AAI27057.1.
    CCDSiCCDS36436.1.
    RefSeqiNP_080440.2. NM_026164.2.
    UniGeneiMm.54126.

    Genome annotation databases

    EnsembliENSMUST00000043082; ENSMUSP00000043286; ENSMUSG00000036257.
    GeneIDi67452.
    KEGGimmu:67452.
    UCSCiuc007nlp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB044139 mRNA. Translation: BAB97200.1 .
    AK004621 mRNA. Translation: BAB23417.1 . Sequence problems.
    AK145776 mRNA. Translation: BAE26645.1 .
    AK163211 mRNA. Translation: BAE37236.1 .
    AK168475 mRNA. Translation: BAE40365.1 .
    BC019364 mRNA. Translation: AAH19364.1 . Different initiation.
    BC127056 mRNA. Translation: AAI27057.1 .
    CCDSi CCDS36436.1.
    RefSeqi NP_080440.2. NM_026164.2.
    UniGenei Mm.54126.

    3D structure databases

    ProteinModelPortali Q8K1N1.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8K1N1.

    Proteomic databases

    MaxQBi Q8K1N1.
    PaxDbi Q8K1N1.
    PRIDEi Q8K1N1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000043082 ; ENSMUSP00000043286 ; ENSMUSG00000036257 .
    GeneIDi 67452.
    KEGGi mmu:67452.
    UCSCi uc007nlp.1. mouse.

    Organism-specific databases

    CTDi 50640.
    MGIi MGI:1914702. Pnpla8.

    Phylogenomic databases

    eggNOGi COG3621.
    GeneTreei ENSGT00530000063645.
    HOGENOMi HOG000115579.
    HOVERGENi HBG102100.
    InParanoidi Q8K1N1.
    KOi K16815.
    OMAi KYDSKSQ.
    PhylomeDBi Q8K1N1.
    TreeFami TF319230.

    Enzyme and pathway databases

    Reactomei REACT_199027. Acyl chain remodelling of PE.
    REACT_199038. Acyl chain remodelling of PC.

    Miscellaneous databases

    NextBioi 324606.
    PROi Q8K1N1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8K1N1.
    Bgeei Q8K1N1.
    CleanExi MM_PNPLA8.
    Genevestigatori Q8K1N1.

    Family and domain databases

    InterProi IPR016035. Acyl_Trfase/lysoPLipase.
    IPR002641. Patatin/PLipase_A2-rel.
    [Graphical view ]
    Pfami PF01734. Patatin. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52151. SSF52151. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A novel intracellular membrane-bound calcium-independent phospholipase A(2)."
      Tanaka H., Takeya R., Sumimoto H.
      Biochem. Biophys. Res. Commun. 272:320-326(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Egg, Kidney and Lung.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary tumor.
    4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-730, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiPLPL8_MOUSE
    AccessioniPrimary (citable) accession number: Q8K1N1
    Secondary accession number(s): Q3TH33, Q8VEC0, Q9DC20
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3