Reviewed, UniProtKB/Swiss-Prot Q8K1M6 (DNM1L_MOUSE)

Names and origin

Protein names

Recommended name:
    Dynamin-1-like protein
    EC=3.6.5.5
Alternative name(s):
    Dynamin-related protein 1
    Dynamin family member proline-rich carboxyl-terminal domain less
      Short name=Dymple

Gene names

Name:	Dnm1l
Synonyms:	Drp1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	742 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions in mitochondrial and peroxisomal division probably by regulating membrane fission. Enzyme hydrolyzing GTP that oligomerizes to form ring-like structures and is able to remodel membranes. May also play a role on organelles of the secretory pathway By similarity.
Catalytic activity	GTP + H2O = GDP + phosphate.
Subunit structure	Homotetramer; N-terminal part binds to the C-terminal part of another DNM1L. Can self-assemble in multimeric ring-like structures. Interacts with GSK3B and FIS1 By similarity.
Subcellular location	Cytoplasm › cytosol By similarity. Endomembrane system; Peripheral membrane protein By similarity. Note: Mainly cytosolic By similarity. Also membrane-associated. Localizes to mitochondria at spots of division events. Associated with peroxisomal membranes, it is recruited in part by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear By similarity.
Tissue specificity	Expressed in the cerebellum and in several regions of the cerebrum and diencephalon. Strongly expressed in the cerebellar Purkinje cells and in the pontile giant neurons. Ref.6
Post-translational modification	Phosphorylated by GSK3B By similarity.
Sequence similarities	Belongs to the dynamin family. Contains 1 GED domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8K1M6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q8K1M6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     539-564: Missing.

Isoform 3 (identifier: Q8K1M6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     85-90: Missing.
     539-575: Missing.

Isoform 4 (identifier: Q8K1M6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-104: Missing.
     105-105: K → M
     539-564: Missing.

Isoform 5 (identifier: Q8K1M6-5)

The sequence of this isoform differs from the canonical sequence as follows:
     85-90: Missing.
     214-227: RRTLAVITKLDLMD → KGRCLYLMDVDLQW
     228-742: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 742	742	Dynamin-1-like protein		PRO_0000206567
Regions
	Domain	650 – 741	92	GED
	Nucleotide binding	32 – 39	8	GTP By similarity
	Nucleotide binding	152 – 156	5	GTP By similarity
	Nucleotide binding	221 – 224	4	GTP By similarity
	Region	1 – 349	349	N-terminal dimerization domain By similarity
	Region	454 – 654	201	Interaction with GSK3B By similarity
	Region	548 – 742	195	C-terminal dimerization domain By similarity
Amino acid modifications
	Modified residue	289	1	N6-acetyllysine By similarity
	Modified residue	535	1	Phosphoserine Ref.7
	Modified residue	622	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 104	104	Missing in isoform 4.		VSP_013689
	Alternative sequence	85 – 90	6	Missing in isoform 3 and isoform 5.		VSP_013690
	Alternative sequence	105	1	K → M in isoform 4.		VSP_013691
	Alternative sequence	214 – 227	14	RRTLA…LDLMD → KGRCLYLMDVDLQW in isoform 5.		VSP_013692
	Alternative sequence	228 – 742	515	Missing in isoform 5.		VSP_013693
	Alternative sequence	539 – 575	37	Missing in isoform 3.		VSP_013694
	Alternative sequence	539 – 564	26	Missing in isoform 2 and isoform 4.		VSP_013695
Experimental info
	Sequence conflict	165	1	P → L in BAC06576. Ref.1
	Sequence conflict	320	1	Q → R in BAC06576. Ref.1
	Sequence conflict	519	1	E → A in BAC34640. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: 5A8679B61A444847	FASTA	742	82,658
	10 20 30 40 50 60 MEALIPVINK LQDVFNTVGA DIIQLPQIVV VGTQSSGKSS VLESLVGRDL LPRGTGVVTR 70 80 90 100 110 120 RPLILQLVHV SPEDKRKTTG EENGKFQSWR VEAEEWGKFL HTKNKLYTDF DEIRQEIENE 130 140 150 160 170 180 TERISGNNKG VSPEPIHLKV FSPNVVNLTL VDLPGMTKVP VGDQPKDIEL QIRELILRFI 190 200 210 220 230 240 SNPNSIILAV TAANTDMATS EALKISREVD PDGRRTLAVI TKLDLMDAGT DAMDVLMGRV 250 260 270 280 290 300 IPVKLGIIGV VNRSQLDINN KKSVTDSIRD EYAFLQKKYP SLANRNGTKY LARTLNRLLM 310 320 330 340 350 360 HHIRDCLPEL KTRINVLAAQ YQSLLNSYGE PVDDKSATLL QLITKFATEY CNTIEGTAKY 370 380 390 400 410 420 IETSELCGGA RICYIFHETF GRTLESVDPL GGLNTIDILT AIRNATGPRP ALFVPEVSFE 430 440 450 460 470 480 LLVKRQIKRL EEPSLRCVEL VHEEMQRIIQ HCSNYSTQEL LRFPKLHDAI VEVVTCLLRK 490 500 510 520 530 540 RLPVTNEMVH NLVAIELAYI NTKHPDFADA CGLMNNNIEE QRRNRLAREL PSAGSRDKSS 550 560 570 580 590 600 KVPSALAPAS QEPPPAASAE ADGKLIQDNR RETKNVPSAG GGIGDGGQEP TTGNWRGMLK 610 620 630 640 650 660 TSKAEELLAE EKSKPIPIMP ASPQKGHAVN LLDVPVPVAR KLSAREQRDC EVIERLIKSY 670 680 690 700 710 720 FLIVRKNIQD SVPKAVMHFL VNHVKDTLQS ELVGQLYKSS LLDDLLTESE DMAQRRKEAA 730 740 DMLKALQGAS QIIAEIRETH LW
	Isoform 2. Checksum: 3C922A7A135B9A37	FASTA	716	80,212
	10 20 30 40 50 60 MEALIPVINK LQDVFNTVGA DIIQLPQIVV VGTQSSGKSS VLESLVGRDL LPRGTGVVTR 70 80 90 100 110 120 RPLILQLVHV SPEDKRKTTG EENGKFQSWR VEAEEWGKFL HTKNKLYTDF DEIRQEIENE 130 140 150 160 170 180 TERISGNNKG VSPEPIHLKV FSPNVVNLTL VDLPGMTKVP VGDQPKDIEL QIRELILRFI 190 200 210 220 230 240 SNPNSIILAV TAANTDMATS EALKISREVD PDGRRTLAVI TKLDLMDAGT DAMDVLMGRV 250 260 270 280 290 300 IPVKLGIIGV VNRSQLDINN KKSVTDSIRD EYAFLQKKYP SLANRNGTKY LARTLNRLLM 310 320 330 340 350 360 HHIRDCLPEL KTRINVLAAQ YQSLLNSYGE PVDDKSATLL QLITKFATEY CNTIEGTAKY 370 380 390 400 410 420 IETSELCGGA RICYIFHETF GRTLESVDPL GGLNTIDILT AIRNATGPRP ALFVPEVSFE 430 440 450 460 470 480 LLVKRQIKRL EEPSLRCVEL VHEEMQRIIQ HCSNYSTQEL LRFPKLHDAI VEVVTCLLRK 490 500 510 520 530 540 RLPVTNEMVH NLVAIELAYI NTKHPDFADA CGLMNNNIEE QRRNRLAREL PSAGSRDKLI 550 560 570 580 590 600 QDNRRETKNV PSAGGGIGDG GQEPTTGNWR GMLKTSKAEE LLAEEKSKPI PIMPASPQKG 610 620 630 640 650 660 HAVNLLDVPV PVARKLSARE QRDCEVIERL IKSYFLIVRK NIQDSVPKAV MHFLVNHVKD 670 680 690 700 710 TLQSELVGQL YKSSLLDDLL TESEDMAQRR KEAADMLKAL QGASQIIAEI RETHLW
	Isoform 3. Checksum: 68ED85D93914B4DC	FASTA	699	78,011
	10 20 30 40 50 60 MEALIPVINK LQDVFNTVGA DIIQLPQIVV VGTQSSGKSS VLESLVGRDL LPRGTGVVTR 70 80 90 100 110 120 RPLILQLVHV SPEDKRKTTG EENGVEAEEW GKFLHTKNKL YTDFDEIRQE IENETERISG 130 140 150 160 170 180 NNKGVSPEPI HLKVFSPNVV NLTLVDLPGM TKVPVGDQPK DIELQIRELI LRFISNPNSI 190 200 210 220 230 240 ILAVTAANTD MATSEALKIS REVDPDGRRT LAVITKLDLM DAGTDAMDVL MGRVIPVKLG 250 260 270 280 290 300 IIGVVNRSQL DINNKKSVTD SIRDEYAFLQ KKYPSLANRN GTKYLARTLN RLLMHHIRDC 310 320 330 340 350 360 LPELKTRINV LAAQYQSLLN SYGEPVDDKS ATLLQLITKF ATEYCNTIEG TAKYIETSEL 370 380 390 400 410 420 CGGARICYIF HETFGRTLES VDPLGGLNTI DILTAIRNAT GPRPALFVPE VSFELLVKRQ 430 440 450 460 470 480 IKRLEEPSLR CVELVHEEMQ RIIQHCSNYS TQELLRFPKL HDAIVEVVTC LLRKRLPVTN 490 500 510 520 530 540 EMVHNLVAIE LAYINTKHPD FADACGLMNN NIEEQRRNRL ARELPSAGSR DKVPSAGGGI 550 560 570 580 590 600 GDGGQEPTTG NWRGMLKTSK AEELLAEEKS KPIPIMPASP QKGHAVNLLD VPVPVARKLS 610 620 630 640 650 660 AREQRDCEVI ERLIKSYFLI VRKNIQDSVP KAVMHFLVNH VKDTLQSELV GQLYKSSLLD 670 680 690 DLLTESEDMA QRRKEAADML KALQGASQII AEIRETHLW
	Isoform 4. Checksum: C5D0BCE4B4622502	FASTA	612	68,701
	10 20 30 40 50 60 MLYTDFDEIR QEIENETERI SGNNKGVSPE PIHLKVFSPN VVNLTLVDLP GMTKVPVGDQ 70 80 90 100 110 120 PKDIELQIRE LILRFISNPN SIILAVTAAN TDMATSEALK ISREVDPDGR RTLAVITKLD 130 140 150 160 170 180 LMDAGTDAMD VLMGRVIPVK LGIIGVVNRS QLDINNKKSV TDSIRDEYAF LQKKYPSLAN 190 200 210 220 230 240 RNGTKYLART LNRLLMHHIR DCLPELKTRI NVLAAQYQSL LNSYGEPVDD KSATLLQLIT 250 260 270 280 290 300 KFATEYCNTI EGTAKYIETS ELCGGARICY IFHETFGRTL ESVDPLGGLN TIDILTAIRN 310 320 330 340 350 360 ATGPRPALFV PEVSFELLVK RQIKRLEEPS LRCVELVHEE MQRIIQHCSN YSTQELLRFP 370 380 390 400 410 420 KLHDAIVEVV TCLLRKRLPV TNEMVHNLVA IELAYINTKH PDFADACGLM NNNIEEQRRN 430 440 450 460 470 480 RLARELPSAG SRDKLIQDNR RETKNVPSAG GGIGDGGQEP TTGNWRGMLK TSKAEELLAE 490 500 510 520 530 540 EKSKPIPIMP ASPQKGHAVN LLDVPVPVAR KLSAREQRDC EVIERLIKSY FLIVRKNIQD 550 560 570 580 590 600 SVPKAVMHFL VNHVKDTLQS ELVGQLYKSS LLDDLLTESE DMAQRRKEAA DMLKALQGAS 610 QIIAEIRETH LW
	Isoform 5. Checksum: 29C66A3A04924F55	FASTA	221	24,488
	10 20 30 40 50 60 MEALIPVINK LQDVFNTVGA DIIQLPQIVV VGTQSSGKSS VLESLVGRDL LPRGTGVVTR 70 80 90 100 110 120 RPLILQLVHV SPEDKRKTTG EENGVEAEEW GKFLHTKNKL YTDFDEIRQE IENETERISG 130 140 150 160 170 180 NNKGVSPEPI HLKVFSPNVV NLTLVDLPGM TKVPVGDQPK DIELQIRELI LRFISNPNSI 190 200 210 220 ILAVTAANTD MATSEALKIS REVDPDGKGR CLYLMDVDLQ W

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Stage-specific enhanced expression of mitochondrial fusion and fission factors during spermatogenesis in rat testis." Honda S., Hirose S. Biochem. Biophys. Res. Commun. 311:424-432(2003) [PubMed: 14592431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Osteoclast.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: C57BL/6J. Tissue: Adipose tissue and Spinal ganglion.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4). Strain: C57BL/6. Tissue: Brain, Mammary gland and Thymus.
[4]	"Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method." Piao Y., Ko N.T., Lim M.K., Ko M.S.H. Genome Res. 11:1553-1558(2001) [PubMed: 11544199] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 448-659 (ISOFORM 1).
[5]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 626-640 AND 725-737, MASS SPECTROMETRY. Tissue: Hippocampus.
[6]	"Dymple, a novel dynamin-like high molecular weight GTPase lacking a proline-rich carboxyl-terminal domain in mammalian cells." Kamimoto T., Nagai Y., Onogi H., Muro Y., Wakabayashi T., Hagiwara M. J. Biol. Chem. 273:1044-1051(1998) [PubMed: 9422767] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 18973353] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-535, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB079133 mRNA. Translation: BAC06576.1. AK051443 mRNA. Translation: BAC34640.1. AK080871 mRNA. Translation: BAC38054.1. BC027538 mRNA. Translation: AAH27538.1. BC040777 mRNA. Translation: AAH40777.1. BC079635 mRNA. Translation: AAH79635.1. CF914619 mRNA. No translation available.
IPI	IPI00172221. IPI00471349. IPI00556723. IPI00556781. IPI00556857.
RefSeq	NP_001021118.1. NP_690029.2.
UniGene	Mm.218820
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8K1M6. 1 interaction.
STRING	Q8K1M6.
PTM databases
PhosphoSite	Q8K1M6.
Proteomic databases
PRIDE	Q8K1M6.
Genome annotation databases
Ensembl	ENSMUST00000023477; ENSMUSP00000023477; ENSMUSG00000022789; Mus musculus. [Genome view] ENSMUST00000047122; ENSMUSP00000093944; ENSMUSG00000022789; Mus musculus. [Genome view] ENSMUST00000115749; ENSMUSP00000111415; ENSMUSG00000022789; Mus musculus. [Genome view]
GeneID	74006.
KEGG	mmu:74006.
UCSC	uc007yik.1. mouse. uc007yil.1. mouse. uc007yin.1. mouse. uc007yio.1. mouse.
Organism-specific databases
CTD	74006.
MGI	MGI:1921256. Dnm1l.
Phylogenomic databases
HOGENOM	Q8K1M6.
HOVERGEN	Q8K1M6.
OMA	NDPATWK.
Enzyme and pathway databases
BRENDA	3.6.5.5. 244.
Gene expression databases
ArrayExpress	Q8K1M6.
Bgee	Q8K1M6.
CleanEx	MM_DNM1L.
Genevestigator	Q8K1M6.
GermOnline	ENSMUSG00000022789. Mus musculus.
Family and domain databases
InterPro	IPR000375. Dynamin_central. IPR001401. Dynamin_GTPase. IPR019762. Dynamin_GTPase_CS. IPR003130. GED. [Graphical view]
Pfam	PF01031. Dynamin_M. 1 hit. PF00350. Dynamin_N. 1 hit. PF02212. GED. 1 hit. [Graphical view]
PRINTS	PR00195. DYNAMIN.
SMART	SM00053. DYNc. 1 hit. SM00302. GED. 1 hit. [Graphical view]
PROSITE	PS00410. DYNAMIN. 1 hit. PS51388. GED. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	339508.
SOURCE	Search...

Entry information

Entry name

DNM1L_MOUSE

Accession

Primary (citable) accession number: Q8K1M6
Secondary accession number(s): Q8BNQ5 , Q8BQ64, Q8CGD0, Q8K1A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 10, 2005
Last modified:	November 3, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)