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Q8K1M4 (GLIS1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein GLIS1
Alternative name(s):
GLI-similar 1
Gli homologous protein 1
Short name=GliH1
Gene names
Name:Glis1
Synonyms:Gli5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length789 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3'. Ref.1 Ref.2

Subcellular location

Nucleus Ref.1.

Tissue specificity

In the adult, expressed highly in placenta and kidney and at lower levels in the testis, brain, colon, brown fat tissue and thymus. During embryo development, expressed in the frontal nasal region, branchial arches, somites, vibrissal and hair follicles, limb buds, craniofacial regions, ventral part of the tail, intervertebral disks, teeth, eyes and kidney. Ref.1 Ref.2

Developmental stage

In the embryo, expression is detected at 10 dpc becoming stronger by 11 dpc and continuing through to 16.5 dpc. Ref.1 Ref.2

Sequence similarities

Belongs to the GLI C2H2-type zinc-finger protein family. Ref.1

Contains 5 C2H2-type zinc fingers.

Sequence caution

The sequence AAH66157.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: Q8K1M4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.2 (identifier: Q8K1M4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-171: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 789789Zinc finger protein GLIS1
PRO_0000047210

Regions

Zinc finger366 – 39126C2H2-type 1
Zinc finger400 – 42728C2H2-type 2; atypical
Zinc finger433 – 45725C2H2-type 3
Zinc finger463 – 48725C2H2-type 4
Zinc finger493 – 51725C2H2-type 5
Motif511 – 52717Bipartite nuclear localization signal Potential
Compositional bias627 – 68054Pro-rich

Natural variations

Alternative sequence1 – 171171Missing in isoform 2. Ref.2
VSP_051623

Experimental info

Sequence conflict1731V → A in AAM93156. Ref.1
Sequence conflict3541G → R in AAM93156. Ref.1
Sequence conflict3781E → G in AAH66157. Ref.3
Sequence conflict3841V → E in AAH66157. Ref.3
Sequence conflict4661Q → R in AAM93156. Ref.1
Sequence conflict6341V → A in AAH66157. Ref.3
Sequence conflict6681Y → S in AAH66157. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 21, 2004. Version 2.
Checksum: 98D87D9EA20D38FD

FASTA78984,172
        10         20         30         40         50         60 
MHCEVAEALS DKRPKEAPGA PGQGRGPVSL GAHMAFRIAV SGGGCGDGNP LDLLPRLPVP 

        70         80         90        100        110        120 
PPRAHDLLRP RSPRDYGVSK TGSGKVNGSY GHSSEKSLLD LDLAEGPSPS CHQGLFLPAG 

       130        140        150        160        170        180 
TPPPRGHPPV CEKLLHFPHP NRSPRPQATF VNGSLPAAQH IKQEALPDYQ AMVSAHTPLP 

       190        200        210        220        230        240 
THCRAPSSMG LPSDLDFPDR GLTNPAPSCY LLGNEPISDL GPQPEAHLPE GSLKRCCLLG 

       250        260        270        280        290        300 
LPPTSSASSS PCASSDINPV IHSSQTALVS CVNGLRSPPL PGDLGGPPKR SRPGPASSDG 

       310        320        330        340        350        360 
QEGSLQLEAC RKSGFLKQEP MDEFSELFAP HHQGLPPPYP LPQLPTGPGL GGLGLGLAGR 

       370        380        390        400        410        420 
MVAGRQACRW VDCCAAYEQQ EELVRHIEKS HIDQRKGEDF TCFWAGCVRR YKPFNARYKL 

       430        440        450        460        470        480 
LIHMRVHSGE KPNKCMFEGC SKAFSRLENL KIHLRSHTGE KPYLCQHPGC QKAFSNSSDR 

       490        500        510        520        530        540 
AKHQRTHLDT KPYACQIPGC SKRYTDPSSL RKHVKAHSAK EQQVRKKLHT GADPEADVLS 

       550        560        570        580        590        600 
ECLSLQQLQA STLLPASRGK GSQTLSQELL PGVYPGSVTP QNGLASGILS PSHDVPSRHH 

       610        620        630        640        650        660 
PLEVPTGSHH HLSPLPTAES TRDGLGPSLL SPMVSPLKGL GPPPLPPASQ SQSPGGQSFS 

       670        680        690        700        710        720 
TVPSKPTYPS FQSPPPLPSP QGYQGSFHSI QNCFPYADCY RATEPAASRD GLVGDAHGFN 

       730        740        750        760        770        780 
PLRPSTYSSL STPLSAPGYE TLAETPCPPA LQPQPAEDLV PSGPEDCGFF PNGAFDHCLS 


HIPSIYTDT 

« Hide

Isoform 2 [UniParc].

Checksum: 25055F3D35CECBF1
Show »

FASTA61866,209

References

« Hide 'large scale' references
[1]"Identification of Glis1, a novel Gli-related, Kruppel-like zinc finger protein containing transactivation and repressor functions."
Kim Y.-S., Lewandoski M., Perantoni A.O., Kurebayashi S., Nakanishi G., Jetten A.M.
J. Biol. Chem. 277:30901-30913(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: BALB/c.
Tissue: Kidney.
[2]"A novel gene, GliH1, with homology to the Gli zinc finger domain not required for mouse development."
Nakashima M., Tanese N., Ito M., Auerbach W., Bai C., Furukawa T., Toyono T., Akamine A., Joyner A.L.
Mech. Dev. 119:21-34(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 116-789 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF486579 mRNA. Translation: AAM93156.1.
AF220434 mRNA. Translation: AAM12150.1.
BC066157 mRNA. Translation: AAH66157.1. Different initiation.
CCDSCCDS38831.1. [Q8K1M4-1]
RefSeqNP_671754.2. NM_147221.2. [Q8K1M4-1]
XP_006503036.1. XM_006502973.1. [Q8K1M4-1]
XP_006503037.1. XM_006502974.1. [Q8K1M4-1]
XP_006503038.1. XM_006502975.1. [Q8K1M4-1]
UniGeneMm.331757.

3D structure databases

ProteinModelPortalQ8K1M4.
SMRQ8K1M4. Positions 338-553.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8K1M4.

Proteomic databases

MaxQBQ8K1M4.
PaxDbQ8K1M4.
PRIDEQ8K1M4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000046005; ENSMUSP00000035650; ENSMUSG00000034762. [Q8K1M4-1]
GeneID230587.
KEGGmmu:230587.
UCSCuc008tzv.1. mouse. [Q8K1M4-1]

Organism-specific databases

CTD148979.
MGIMGI:2386723. Glis1.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00740000114893.
HOGENOMHOG000231136.
HOVERGENHBG051736.
InParanoidQ8K1M4.
KOK09232.
OMALRPNGYH.
OrthoDBEOG70S75H.
PhylomeDBQ8K1M4.
TreeFamTF350216.

Gene expression databases

BgeeQ8K1M4.
CleanExMM_GLIS1.
GenevestigatorQ8K1M4.

Family and domain databases

Gene3D3.30.160.60. 5 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio379951.
PROQ8K1M4.
SOURCESearch...

Entry information

Entry nameGLIS1_MOUSE
AccessionPrimary (citable) accession number: Q8K1M4
Secondary accession number(s): Q6NZF6, Q8R4Z3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: July 9, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot