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Q8K1M4

- GLIS1_MOUSE

UniProt

Q8K1M4 - GLIS1_MOUSE

Protein

Zinc finger protein GLIS1

Gene

Glis1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 2 (21 Dec 2004)
      Previous versions | rss
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    Functioni

    Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3'.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri366 – 39126C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri400 – 42728C2H2-type 2; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri433 – 45725C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri463 – 48725C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri493 – 51725C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. metal ion binding Source: UniProtKB-KW
    3. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
    4. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB
    5. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB

    GO - Biological processi

    1. negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
    2. positive regulation of transcription, DNA-templated Source: MGI
    3. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    4. regulation of transcription from RNA polymerase II promoter Source: MGI

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger protein GLIS1
    Alternative name(s):
    GLI-similar 1
    Gli homologous protein 1
    Short name:
    GliH1
    Gene namesi
    Name:Glis1Imported
    Synonyms:Gli5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:2386723. Glis1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 789789Zinc finger protein GLIS1PRO_0000047210Add
    BLAST

    Proteomic databases

    MaxQBiQ8K1M4.
    PaxDbiQ8K1M4.
    PRIDEiQ8K1M4.

    PTM databases

    PhosphoSiteiQ8K1M4.

    Expressioni

    Tissue specificityi

    In the adult, expressed highly in placenta and kidney and at lower levels in the testis, brain, colon, brown fat tissue and thymus. During embryo development, expressed in the frontal nasal region, branchial arches, somites, vibrissal and hair follicles, limb buds, craniofacial regions, ventral part of the tail, intervertebral disks, teeth, eyes and kidney.2 Publications

    Developmental stagei

    In the embryo, expression is detected at 10 dpc becoming stronger by 11 dpc and continuing through to 16.5 dpc.2 Publications

    Gene expression databases

    BgeeiQ8K1M4.
    CleanExiMM_GLIS1.
    GenevestigatoriQ8K1M4.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K1M4.
    SMRiQ8K1M4. Positions 338-553.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi511 – 52717Bipartite nuclear localization signalSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi627 – 68054Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri366 – 39126C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri400 – 42728C2H2-type 2; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri433 – 45725C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri463 – 48725C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri493 – 51725C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00740000114893.
    HOGENOMiHOG000231136.
    HOVERGENiHBG051736.
    InParanoidiQ8K1M4.
    KOiK09232.
    OMAiLRPNGYH.
    OrthoDBiEOG70S75H.
    PhylomeDBiQ8K1M4.
    TreeFamiTF350216.

    Family and domain databases

    Gene3Di3.30.160.60. 5 hits.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 5 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 4 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 11 Publication (identifier: Q8K1M4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MHCEVAEALS DKRPKEAPGA PGQGRGPVSL GAHMAFRIAV SGGGCGDGNP    50
    LDLLPRLPVP PPRAHDLLRP RSPRDYGVSK TGSGKVNGSY GHSSEKSLLD 100
    LDLAEGPSPS CHQGLFLPAG TPPPRGHPPV CEKLLHFPHP NRSPRPQATF 150
    VNGSLPAAQH IKQEALPDYQ AMVSAHTPLP THCRAPSSMG LPSDLDFPDR 200
    GLTNPAPSCY LLGNEPISDL GPQPEAHLPE GSLKRCCLLG LPPTSSASSS 250
    PCASSDINPV IHSSQTALVS CVNGLRSPPL PGDLGGPPKR SRPGPASSDG 300
    QEGSLQLEAC RKSGFLKQEP MDEFSELFAP HHQGLPPPYP LPQLPTGPGL 350
    GGLGLGLAGR MVAGRQACRW VDCCAAYEQQ EELVRHIEKS HIDQRKGEDF 400
    TCFWAGCVRR YKPFNARYKL LIHMRVHSGE KPNKCMFEGC SKAFSRLENL 450
    KIHLRSHTGE KPYLCQHPGC QKAFSNSSDR AKHQRTHLDT KPYACQIPGC 500
    SKRYTDPSSL RKHVKAHSAK EQQVRKKLHT GADPEADVLS ECLSLQQLQA 550
    STLLPASRGK GSQTLSQELL PGVYPGSVTP QNGLASGILS PSHDVPSRHH 600
    PLEVPTGSHH HLSPLPTAES TRDGLGPSLL SPMVSPLKGL GPPPLPPASQ 650
    SQSPGGQSFS TVPSKPTYPS FQSPPPLPSP QGYQGSFHSI QNCFPYADCY 700
    RATEPAASRD GLVGDAHGFN PLRPSTYSSL STPLSAPGYE TLAETPCPPA 750
    LQPQPAEDLV PSGPEDCGFF PNGAFDHCLS HIPSIYTDT 789
    Length:789
    Mass (Da):84,172
    Last modified:December 21, 2004 - v2
    Checksum:i98D87D9EA20D38FD
    GO
    Isoform 21 Publication (identifier: Q8K1M4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-171: Missing.

    Show »
    Length:618
    Mass (Da):66,209
    Checksum:i25055F3D35CECBF1
    GO

    Sequence cautioni

    The sequence AAH66157.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti173 – 1731V → A in AAM93156. (PubMed:12042312)Curated
    Sequence conflicti354 – 3541G → R in AAM93156. (PubMed:12042312)Curated
    Sequence conflicti378 – 3781E → G in AAH66157. (PubMed:15489334)Curated
    Sequence conflicti384 – 3841V → E in AAH66157. (PubMed:15489334)Curated
    Sequence conflicti466 – 4661Q → R in AAM93156. (PubMed:12042312)Curated
    Sequence conflicti634 – 6341V → A in AAH66157. (PubMed:15489334)Curated
    Sequence conflicti668 – 6681Y → S in AAH66157. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 171171Missing in isoform 2. 1 PublicationVSP_051623Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF486579 mRNA. Translation: AAM93156.1.
    AF220434 mRNA. Translation: AAM12150.1.
    BC066157 mRNA. Translation: AAH66157.1. Different initiation.
    CCDSiCCDS38831.1. [Q8K1M4-1]
    RefSeqiNP_671754.2. NM_147221.2. [Q8K1M4-1]
    XP_006503036.1. XM_006502973.1. [Q8K1M4-1]
    XP_006503037.1. XM_006502974.1. [Q8K1M4-1]
    XP_006503038.1. XM_006502975.1. [Q8K1M4-1]
    UniGeneiMm.331757.

    Genome annotation databases

    EnsembliENSMUST00000046005; ENSMUSP00000035650; ENSMUSG00000034762. [Q8K1M4-1]
    GeneIDi230587.
    KEGGimmu:230587.
    UCSCiuc008tzv.1. mouse. [Q8K1M4-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF486579 mRNA. Translation: AAM93156.1 .
    AF220434 mRNA. Translation: AAM12150.1 .
    BC066157 mRNA. Translation: AAH66157.1 . Different initiation.
    CCDSi CCDS38831.1. [Q8K1M4-1 ]
    RefSeqi NP_671754.2. NM_147221.2. [Q8K1M4-1 ]
    XP_006503036.1. XM_006502973.1. [Q8K1M4-1 ]
    XP_006503037.1. XM_006502974.1. [Q8K1M4-1 ]
    XP_006503038.1. XM_006502975.1. [Q8K1M4-1 ]
    UniGenei Mm.331757.

    3D structure databases

    ProteinModelPortali Q8K1M4.
    SMRi Q8K1M4. Positions 338-553.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8K1M4.

    Proteomic databases

    MaxQBi Q8K1M4.
    PaxDbi Q8K1M4.
    PRIDEi Q8K1M4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000046005 ; ENSMUSP00000035650 ; ENSMUSG00000034762 . [Q8K1M4-1 ]
    GeneIDi 230587.
    KEGGi mmu:230587.
    UCSCi uc008tzv.1. mouse. [Q8K1M4-1 ]

    Organism-specific databases

    CTDi 148979.
    MGIi MGI:2386723. Glis1.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00740000114893.
    HOGENOMi HOG000231136.
    HOVERGENi HBG051736.
    InParanoidi Q8K1M4.
    KOi K09232.
    OMAi LRPNGYH.
    OrthoDBi EOG70S75H.
    PhylomeDBi Q8K1M4.
    TreeFami TF350216.

    Miscellaneous databases

    NextBioi 379951.
    PROi Q8K1M4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8K1M4.
    CleanExi MM_GLIS1.
    Genevestigatori Q8K1M4.

    Family and domain databases

    Gene3Di 3.30.160.60. 5 hits.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 5 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of Glis1, a novel Gli-related, Kruppel-like zinc finger protein containing transactivation and repressor functions."
      Kim Y.-S., Lewandoski M., Perantoni A.O., Kurebayashi S., Nakanishi G., Jetten A.M.
      J. Biol. Chem. 277:30901-30913(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: BALB/cImported.
      Tissue: Kidney1 Publication.
    2. "A novel gene, GliH1, with homology to the Gli zinc finger domain not required for mouse development."
      Nakashima M., Tanese N., Ito M., Auerbach W., Bai C., Furukawa T., Toyono T., Akamine A., Joyner A.L.
      Mech. Dev. 119:21-34(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 116-789 (ISOFORM 1).
      Strain: C57BL/6JImported.
      Tissue: KidneyImported.

    Entry informationi

    Entry nameiGLIS1_MOUSE
    AccessioniPrimary (citable) accession number: Q8K1M4
    Secondary accession number(s): Q6NZF6, Q8R4Z3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 21, 2004
    Last sequence update: December 21, 2004
    Last modified: October 1, 2014
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3