ID   SPIN4_MOUSE             Reviewed;         249 AA.
AC   Q8K1L2; Q8BXD0;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   24-JAN-2024, entry version 128.
DE   RecName: Full=Spindlin-4;
GN   Name=Spin4;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J, and NOD; TISSUE=Cerebellum, and Head;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Exhibits H3K4me3-binding activity.
CC       {ECO:0000250|UniProtKB:Q56A73}.
CC   -!- SUBUNIT: Interacts with C11orf84/SPINDOC.
CC       {ECO:0000250|UniProtKB:Q56A73}.
CC   -!- SIMILARITY: Belongs to the SPIN/STSY family. {ECO:0000305}.
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DR   EMBL; AK036158; BAC29325.1; -; mRNA.
DR   EMBL; AK043030; BAC31440.1; -; mRNA.
DR   EMBL; AK047896; BAC33186.1; -; mRNA.
DR   EMBL; AK147852; BAE28181.1; -; mRNA.
DR   EMBL; AK155170; BAE33091.1; -; mRNA.
DR   EMBL; BX470092; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC027796; AAH27796.1; -; mRNA.
DR   CCDS; CCDS30282.1; -.
DR   RefSeq; NP_848868.1; NM_178753.4.
DR   AlphaFoldDB; Q8K1L2; -.
DR   SMR; Q8K1L2; -.
DR   STRING; 10090.ENSMUSP00000094095; -.
DR   PhosphoSitePlus; Q8K1L2; -.
DR   EPD; Q8K1L2; -.
DR   MaxQB; Q8K1L2; -.
DR   PaxDb; 10090-ENSMUSP00000094095; -.
DR   PeptideAtlas; Q8K1L2; -.
DR   ProteomicsDB; 258721; -.
DR   Pumba; Q8K1L2; -.
DR   Antibodypedia; 52266; 95 antibodies from 14 providers.
DR   DNASU; 270624; -.
DR   Ensembl; ENSMUST00000096367.5; ENSMUSP00000094095.4; ENSMUSG00000071722.5.
DR   GeneID; 270624; -.
DR   KEGG; mmu:270624; -.
DR   UCSC; uc009tts.2; mouse.
DR   AGR; MGI:2444925; -.
DR   CTD; 139886; -.
DR   MGI; MGI:2444925; Spin4.
DR   VEuPathDB; HostDB:ENSMUSG00000071722; -.
DR   eggNOG; ENOG502QRYD; Eukaryota.
DR   GeneTree; ENSGT00950000182925; -.
DR   HOGENOM; CLU_068595_0_0_1; -.
DR   InParanoid; Q8K1L2; -.
DR   OMA; HGKKDEW; -.
DR   OrthoDB; 3949689at2759; -.
DR   PhylomeDB; Q8K1L2; -.
DR   TreeFam; TF332665; -.
DR   BioGRID-ORCS; 270624; 2 hits in 76 CRISPR screens.
DR   PRO; PR:Q8K1L2; -.
DR   Proteomes; UP000000589; Chromosome X.
DR   RNAct; Q8K1L2; Protein.
DR   Bgee; ENSMUSG00000071722; Expressed in vas deferens and 140 other cell types or tissues.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR   GO; GO:0035064; F:methylated histone binding; ISS:UniProtKB.
DR   GO; GO:0007276; P:gamete generation; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; IBA:GO_Central.
DR   Gene3D; 2.80.10.70; Spindlin/Ssty; 1.
DR   InterPro; IPR003671; SPIN/Ssty.
DR   InterPro; IPR042567; SPIN/Ssty_sf.
DR   PANTHER; PTHR10405; SPINDLIN; 1.
DR   PANTHER; PTHR10405:SF9; SPINDLIN-4; 1.
DR   Pfam; PF02513; Spin-Ssty; 3.
DR   Genevisible; Q8K1L2; MM.
PE   2: Evidence at transcript level;
KW   Reference proteome.
FT   CHAIN           1..249
FT                   /note="Spindlin-4"
FT                   /id="PRO_0000313795"
FT   REGION          41..90
FT                   /note="Tudor-like domain 1"
FT                   /evidence="ECO:0000255"
FT   REGION          80..85
FT                   /note="Histone H3K4me3 and H3R8me2a binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y657"
FT   REGION          119..168
FT                   /note="Tudor-like domain 2"
FT                   /evidence="ECO:0000255"
FT   REGION          128
FT                   /note="Histone H3K4me3 and H3R8me2a binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y657"
FT   REGION          201..246
FT                   /note="Tudor-like domain 3"
FT                   /evidence="ECO:0000255"
FT   REGION          237..239
FT                   /note="Histone H3K4me3 and H3R8me2a binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y657"
FT   SITE            159
FT                   /note="Histone H3K4me3 and H3R8me2a binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y657"
FT   SITE            170
FT                   /note="Histone H3K4me3 and H3R8me2a binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y657"
FT   CONFLICT        41
FT                   /note="V -> G (in Ref. 1; BAC33186)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   249 AA;  28508 MW;  0B0485959A4A2F10 CRC64;
     MSPPTVPPTG VDGVSAYLMK KRHTHKKQRR KPTFLTHRNI VGCRIQHGWK EGNEPVEQWK
     GTVLEQVSVK PTLYIIKYDG KDSVYGLELH RDKRVLALEI LPERVPSPRI DSRLADSLVG
     KAVGHVFEGE HGKKDEWKGM VLARAPIMDT WFYITYEKDP VLYMYTLLDD YKDGDLRIIP
     DSNYYFPAAE QEPGEVLDSL VGKQVEHAKD DGSKRTGIFI HQVVAKPSVY FIKFDDDIHI
     YVYGLVKTP
//