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Q8K1J6

- TRNT1_MOUSE

UniProt

Q8K1J6 - TRNT1_MOUSE

Protein

CCA tRNA nucleotidyltransferase 1, mitochondrial

Gene

Trnt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Adds and repairs the conserved 3'-CCA sequence necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates.By similarity

    Catalytic activityi

    A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.

    Cofactori

    Magnesium.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei77 – 771By similarity
    Active sitei79 – 791By similarity
    Active sitei121 – 1211By similarity

    GO - Molecular functioni

    1. ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. CTP:3'-cytidine-tRNA cytidylyltransferase activity Source: UniProtKB-EC
    4. CTP:tRNA cytidylyltransferase activity Source: UniProtKB-EC
    5. tRNA adenylyltransferase activity Source: UniProtKB
    6. tRNA binding Source: UniProtKB
    7. tRNA nucleotidyltransferase activity Source: MGI

    GO - Biological processi

    1. tRNA 3'-end processing Source: UniProtKB
    2. tRNA 3'-terminal CCA addition Source: UniProtKB-EC
    3. tRNA processing Source: MGI

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CCA tRNA nucleotidyltransferase 1, mitochondrial (EC:2.7.7.72)
    Alternative name(s):
    mitochondrial tRNA nucleotidyl transferase, CCA-adding
    Short name:
    mt CCA-adding enzyme
    Short name:
    mt tRNA CCA-diphosphorylase
    Short name:
    mt tRNA CCA-pyrophosphorylase
    Short name:
    mt tRNA adenylyltransferase
    Gene namesi
    Name:Trnt1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1917297. Trnt1.

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. centrosome Source: Ensembl
    2. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4141MitochondrionSequence AnalysisAdd
    BLAST
    Chaini42 – 434393CCA tRNA nucleotidyltransferase 1, mitochondrialPRO_0000004783Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei400 – 4001PhosphoserineBy similarity
    Modified residuei402 – 4021N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ8K1J6.
    PaxDbiQ8K1J6.
    PRIDEiQ8K1J6.

    PTM databases

    PhosphoSiteiQ8K1J6.

    Expressioni

    Gene expression databases

    BgeeiQ8K1J6.
    CleanExiMM_TRNT1.
    GenevestigatoriQ8K1J6.

    Interactioni

    Subunit structurei

    Monomer and homodimer.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000108874.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K1J6.
    SMRiQ8K1J6. Positions 30-431.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0617.
    GeneTreeiENSGT00390000009678.
    HOGENOMiHOG000253345.
    HOVERGENiHBG061403.
    InParanoidiQ8K1J6.
    KOiK00974.
    OMAiKGSCDID.
    OrthoDBiEOG7CCBR5.
    PhylomeDBiQ8K1J6.
    TreeFamiTF313253.

    Family and domain databases

    InterProiIPR002646. PolA_pol_head_dom.
    [Graphical view]
    PfamiPF01743. PolyA_pol. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8K1J6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MQSVLYPWHR QVLRCSWSRL CLLKRYLFTM KLQSPEFQSL FTEGLKSLTE    50
    LFAKENHELR IAGGAVRDLL NGVKPQDVDF ATTATPTQMK EMFQSAGIRM 100
    INNKGEKHGT ITARLHEENF EVTTLRIDVT TDGRHAEVEF TTDWQKDAER 150
    RDLTINSMFL GFDGTLFDYF NGYADLKNKK VRFVGHAKQR IQEDYLRILR 200
    YFRFYGRIVD RPGDHDHETL EAIAENAKGL AGISGERIWV ELKKILTGDH 250
    VNHLIHLIYD LGVAPHIGLP ANANLEEFNK VSKNVEGFSP KPMTLLASLF 300
    KVQDDVTKLD LRLKISKEEK NLGLFIVKNR KDLIKATDSS EPLKPYQDFV 350
    IDSREPDATA RVCELLKYQG EHGLLKEMQQ WSVPPFPVSG HDIRKVGISS 400
    GKEIGALLQQ LREQWKKSGY RMEKDELLSY IKKT 434
    Length:434
    Mass (Da):49,895
    Last modified:October 1, 2002 - v1
    Checksum:i0578FB438165838B
    GO
    Isoform 2 (identifier: Q8K1J6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         204-215: FYGRIVDRPGDH → PGIVLGDLTTEK
         216-434: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:215
    Mass (Da):25,018
    Checksum:iB0AEFBB90317372B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti202 – 2021F → I in BAB70663. (PubMed:11504732)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei204 – 21512FYGRI…RPGDH → PGIVLGDLTTEK in isoform 2. 1 PublicationVSP_018616Add
    BLAST
    Alternative sequencei216 – 434219Missing in isoform 2. 1 PublicationVSP_018617Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB063106 mRNA. Translation: BAB70663.1.
    AK011764 mRNA. Translation: BAB27827.3.
    AK135791 mRNA. Translation: BAE22664.1.
    AK152739 mRNA. Translation: BAE31459.1.
    AK166804 mRNA. Translation: BAE39032.1.
    BC031764 mRNA. Translation: AAH31764.1.
    CCDSiCCDS20397.1. [Q8K1J6-1]
    CCDS57443.1. [Q8K1J6-2]
    RefSeqiNP_001229287.1. NM_001242358.1. [Q8K1J6-1]
    NP_001229289.1. NM_001242360.1. [Q8K1J6-2]
    NP_081572.1. NM_027296.3. [Q8K1J6-1]
    XP_006506643.1. XM_006506580.1. [Q8K1J6-1]
    XP_006506644.1. XM_006506581.1. [Q8K1J6-1]
    UniGeneiMm.196332.

    Genome annotation databases

    EnsembliENSMUST00000057578; ENSMUSP00000060900; ENSMUSG00000013736. [Q8K1J6-1]
    ENSMUST00000113247; ENSMUSP00000108873; ENSMUSG00000013736. [Q8K1J6-2]
    ENSMUST00000113248; ENSMUSP00000108874; ENSMUSG00000013736. [Q8K1J6-1]
    ENSMUST00000113249; ENSMUSP00000108875; ENSMUSG00000013736. [Q8K1J6-1]
    GeneIDi70047.
    KEGGimmu:70047.
    UCSCiuc009dcy.2. mouse. [Q8K1J6-1]
    uc029vxx.1. mouse. [Q8K1J6-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB063106 mRNA. Translation: BAB70663.1 .
    AK011764 mRNA. Translation: BAB27827.3 .
    AK135791 mRNA. Translation: BAE22664.1 .
    AK152739 mRNA. Translation: BAE31459.1 .
    AK166804 mRNA. Translation: BAE39032.1 .
    BC031764 mRNA. Translation: AAH31764.1 .
    CCDSi CCDS20397.1. [Q8K1J6-1 ]
    CCDS57443.1. [Q8K1J6-2 ]
    RefSeqi NP_001229287.1. NM_001242358.1. [Q8K1J6-1 ]
    NP_001229289.1. NM_001242360.1. [Q8K1J6-2 ]
    NP_081572.1. NM_027296.3. [Q8K1J6-1 ]
    XP_006506643.1. XM_006506580.1. [Q8K1J6-1 ]
    XP_006506644.1. XM_006506581.1. [Q8K1J6-1 ]
    UniGenei Mm.196332.

    3D structure databases

    ProteinModelPortali Q8K1J6.
    SMRi Q8K1J6. Positions 30-431.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000108874.

    PTM databases

    PhosphoSitei Q8K1J6.

    Proteomic databases

    MaxQBi Q8K1J6.
    PaxDbi Q8K1J6.
    PRIDEi Q8K1J6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000057578 ; ENSMUSP00000060900 ; ENSMUSG00000013736 . [Q8K1J6-1 ]
    ENSMUST00000113247 ; ENSMUSP00000108873 ; ENSMUSG00000013736 . [Q8K1J6-2 ]
    ENSMUST00000113248 ; ENSMUSP00000108874 ; ENSMUSG00000013736 . [Q8K1J6-1 ]
    ENSMUST00000113249 ; ENSMUSP00000108875 ; ENSMUSG00000013736 . [Q8K1J6-1 ]
    GeneIDi 70047.
    KEGGi mmu:70047.
    UCSCi uc009dcy.2. mouse. [Q8K1J6-1 ]
    uc029vxx.1. mouse. [Q8K1J6-2 ]

    Organism-specific databases

    CTDi 51095.
    MGIi MGI:1917297. Trnt1.

    Phylogenomic databases

    eggNOGi COG0617.
    GeneTreei ENSGT00390000009678.
    HOGENOMi HOG000253345.
    HOVERGENi HBG061403.
    InParanoidi Q8K1J6.
    KOi K00974.
    OMAi KGSCDID.
    OrthoDBi EOG7CCBR5.
    PhylomeDBi Q8K1J6.
    TreeFami TF313253.

    Miscellaneous databases

    NextBioi 330891.
    PROi Q8K1J6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8K1J6.
    CleanExi MM_TRNT1.
    Genevestigatori Q8K1J6.

    Family and domain databases

    InterProi IPR002646. PolA_pol_head_dom.
    [Graphical view ]
    Pfami PF01743. PolyA_pol. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of mammalian mitochondrial tRNA nucleotidyltransferases."
      Nagaike T., Suzuki T., Tomari Y., Takemoto-Hori C., Negayama F., Watanabe K., Ueda T.
      J. Biol. Chem. 276:40041-40049(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Blastocyst, Bone marrow macrophage, Egg and Embryo.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Mammary gland.
    4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-402, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiTRNT1_MOUSE
    AccessioniPrimary (citable) accession number: Q8K1J6
    Secondary accession number(s): Q3TKW1
    , Q3UX99, Q920N6, Q9CSX0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2003
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3