Q8K1I7 (WIPF1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
WAS/WASL-interacting protein family member 1

Alternative name(s):
Wiskott-Aldrich syndrome protein-interacting protein
Short name=WASP-interacting protein

Gene names

Name:	Wipf1
Synonyms:	Waspip, Wip

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation By similarity. Plays a role in the formation of cell ruffles. Ref.1 Ref.6
Subunit structure	Binds to WAS within the N-terminal region, at a site distinct from the CDC42-binding site. Binds profilin and actin By similarity. Binds to WASL. Interacts with DBNL. Ref.1 Ref.6
Subcellular location	Cytoplasmic vesicle By similarity. Cytoplasm › cytoskeleton By similarity. Cell projection › ruffle. Note: Vesicle surfaces and along actin tails. Colocalizes with actin stress fibers. When coexpressed with WASL, no longer associated with actin filaments but accumulated in perinuclear and cortical areas like WASL By similarity. Ref.1 Ref.6
Miscellaneous	Recruited to PIP5K-induced vesicle surfaces in the absence of functional WASL.
Sequence similarities	Belongs to the verprolin family. Contains 1 WH2 domain.

Ontologies

Keywords
Cellular component	Cell projection Cytoplasm Cytoplasmic vesicle Cytoskeleton
Domain	Repeat
Ligand	Actin-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton organization Inferred from electronic annotation. Source: Compara actin filament-based movement Inferred from direct assay Ref.1. Source: MGI positive regulation of protein export from nucleus Inferred from electronic annotation. Source: Compara response to other organism Inferred from mutant phenotype PubMed 22966049. Source: MGI
Cellular_component	actin filament Inferred from direct assay PubMed 22966049. Source: MGI cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Was	P70315	3	EBI-644216,EBI-644195

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	WAS/WASL-interacting protein family member 1		PRO_0000065942
Regions
	Domain	32 – 49	18	WH2
	Repeat	342 – 351	10	XRSGPXPPXP motif 1
	Repeat	364 – 373	10	XRSGPXPPXP motif 2
	Repeat	400 – 409	10	XRSGPXPPXP motif 3
	Region	45 – 48	4	Binds actin By similarity
	Compositional bias	2 – 13	12	Poly-Pro
	Compositional bias	64 – 108	45	Gly-rich
	Compositional bias	127 – 492	366	Pro-rich
Amino acid modifications
	Modified residue	268	1	Phosphoserine Ref.5
	Modified residue	330	1	Phosphoserine By similarity
	Modified residue	332	1	Phosphothreonine Ref.4
	Modified residue	340	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8K1I7 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 0BADBE69463B5960	FASTA	493	50,080
	10 20 30 40 50 60 MPVPPPPAPP PPPTFALANT EKPTLNKTEQ AGRNALLSDI SKGKKLKKTV TNDRSAPILD 70 80 90 100 110 120 KPKGAGASAG GYGGGGGGGG GGGGGGGGSG GNFGGGGPPG LGGLFQAGMP KLRSTANRDN 130 140 150 160 170 180 DSGGSRPPIL PPGGRATSAK PFSPPSGPGR FPAPSPGHRS GPPEPPRNRM PPPRPDVGSK 190 200 210 220 230 240 PDSLPPPVPN TPRPVPSSLH NRGSPAGLGA PRPPFPGNRG AAFGAGSARQ NPSGSSSPFP 250 260 270 280 290 300 RPPLPPTPSR ALDDKPPPPP PPVGNRPSMH REAVPPPPSQ TSKPPVPSTP RPGLGSQAPP 310 320 330 340 350 360 PPPPPSRPGP PPLPPASNDE IPRLPQRNLS LTSSAPPLPS PGRSGPLPPP PSERPPPPVR 370 380 390 400 410 420 DPPGRSGPLP PPPPINRNGS TARALPATPQ LPSRSGMDSP RSGPRPPLPP DRPGAGAPPP 430 440 450 460 470 480 PPPSTSVRNG FQDSSCEDEW ESRFYFHPIS DLPPPEPYVP TTKTYPSKLA RNESRSGSNR 490 RERGAPPLPP IPR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Phosphatidylinositol 4,5-biphosphate (PIP2)-induced vesicle movement depends on N-WASP and involves Nck, WIP, and Grb2." Benesch S., Lommel S., Steffen A., Stradal T.E.B., Scaplehorn N., Way M., Wehland J., Rottner K. J. Biol. Chem. 277:37771-37776(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH WASL. Strain: C57BL/6. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Spleen and Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Limb.
[4]	"Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line." Shu H., Chen S., Bi Q., Mumby M., Brekken D.L. Mol. Cell. Proteomics 3:279-286(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-332 AND SER-340, MASS SPECTROMETRY. Tissue: B-cell lymphoma.
[5]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268, MASS SPECTROMETRY. Tissue: Macrophage.
[6]	"Actin-binding protein-1 interacts with WASp-interacting protein to regulate growth factor-induced dorsal ruffle formation." Cortesio C.L., Perrin B.J., Bennin D.A., Huttenlocher A. Mol. Biol. Cell 21:186-197(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DBNL.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ437262 mRNA. Translation: CAD24773.1. AK031046 mRNA. Translation: BAC27228.1. AK156547 mRNA. Translation: BAE33753.1. BC049788 mRNA. Translation: AAH49788.1.
IPI	IPI00169768.
RefSeq	NP_694778.1. NM_153138.3.
UniGene	Mm.223504.
3D structure databases
ProteinModelPortal	Q8K1I7.
SMR	Q8K1I7. Positions 29-60, 451-478.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8K1I7. 5 interactions.
MINT	MINT-260373.
PTM databases
PhosphoSite	Q8K1I7.
Proteomic databases
PaxDb	Q8K1I7.
PRIDE	Q8K1I7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000094681; ENSMUSP00000092268; ENSMUSG00000075284. ENSMUST00000102679; ENSMUSP00000099740; ENSMUSG00000075284. ENSMUST00000102680; ENSMUSP00000099741; ENSMUSG00000075284.
GeneID	215280.
KEGG	mmu:215280.
Organism-specific databases
CTD	7456.
MGI	MGI:2178801. Wipf1.
Phylogenomic databases
eggNOG	NOG149184.
GeneTree	ENSGT00690000102034.
HOGENOM	HOG000231914.
HOVERGEN	HBG054157.
InParanoid	Q8K1I7.
OMA	ALDDKPP.
OrthoDB	EOG49CQ90.
Gene expression databases
ArrayExpress	Q8K1I7.
Bgee	Q8K1I7.
CleanEx	MM_WIPF1.
Genevestigator	Q8K1I7.
GermOnline	ENSMUSG00000075284. Mus musculus.
Family and domain databases
InterPro	IPR003124. WH2_dom. [Graphical view]
Pfam	PF02205. WH2. 1 hit. [Graphical view]
SMART	SM00246. WH2. 1 hit. [Graphical view]
PROSITE	PS51082. WH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	374662.
SOURCE	Search...

Entry information

Entry name

WIPF1_MOUSE

Accession

Primary (citable) accession number: Q8K1I7
Secondary accession number(s): Q3U0U8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program