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Q8K1I7

- WIPF1_MOUSE

UniProt

Q8K1I7 - WIPF1_MOUSE

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Protein

WAS/WASL-interacting protein family member 1

Gene

Wipf1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation By similarity. Plays a role in the formation of cell ruffles.By similarity2 Publications

GO - Biological processi

  1. actin filament-based movement Source: MGI
  2. actin polymerization or depolymerization Source: InterPro
  3. response to other organism Source: MGI
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiREACT_198374. Regulation of actin dynamics for phagocytic cup formation.

Names & Taxonomyi

Protein namesi
Recommended name:
WAS/WASL-interacting protein family member 1
Alternative name(s):
Wiskott-Aldrich syndrome protein-interacting protein
Short name:
WASP-interacting protein
Gene namesi
Name:Wipf1
Synonyms:Waspip, Wip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:2178801. Wipf1.

Subcellular locationi

Cytoplasmic vesicle By similarity. Cytoplasmcytoskeleton By similarity. Cell projectionruffle 2 Publications
Note: Vesicle surfaces and along actin tails. Colocalizes with actin stress fibers. When coexpressed with WASL, no longer associated with actin filaments but accumulated in perinuclear and cortical areas like WASL By similarity.By similarity

GO - Cellular componenti

  1. actin cytoskeleton Source: MGI
  2. actin filament Source: MGI
  3. cell projection Source: UniProtKB-KW
  4. cytoplasmic vesicle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493WAS/WASL-interacting protein family member 1PRO_0000065942Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei143 – 1431Phosphoserine1 Publication
Modified residuei330 – 3301Phosphoserine1 Publication
Modified residuei340 – 3401PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8K1I7.
PaxDbiQ8K1I7.
PRIDEiQ8K1I7.

PTM databases

PhosphoSiteiQ8K1I7.

Expressioni

Gene expression databases

BgeeiQ8K1I7.
CleanExiMM_WIPF1.
ExpressionAtlasiQ8K1I7. baseline and differential.
GenevestigatoriQ8K1I7.

Interactioni

Subunit structurei

Binds to WAS within the N-terminal region, at a site distinct from the CDC42-binding site. Binds profilin and actin By similarity. Binds to WASL. Interacts with DBNL.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
WasP703153EBI-644216,EBI-644195

Protein-protein interaction databases

BioGridi229611. 1 interaction.
IntActiQ8K1I7. 6 interactions.
MINTiMINT-260373.

Structurei

3D structure databases

ProteinModelPortaliQ8K1I7.
SMRiQ8K1I7. Positions 29-60, 451-478.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 4918WH2PROSITE-ProRule annotationAdd
BLAST
Repeati342 – 35110XRSGPXPPXP motif 1
Repeati364 – 37310XRSGPXPPXP motif 2
Repeati400 – 40910XRSGPXPPXP motif 3

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni45 – 484Binds actinBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 1312Poly-ProAdd
BLAST
Compositional biasi64 – 10845Gly-richAdd
BLAST
Compositional biasi127 – 492366Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the verprolin family.Curated
Contains 1 WH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG149184.
GeneTreeiENSGT00760000119406.
HOGENOMiHOG000231914.
HOVERGENiHBG054157.
InParanoidiQ8K1I7.
OMAiQSSLHNR.
OrthoDBiEOG7PS1GQ.
PhylomeDBiQ8K1I7.
TreeFamiTF332135.

Family and domain databases

InterProiIPR003124. WH2_dom.
IPR028295. WIP.
[Graphical view]
PANTHERiPTHR23202:SF30. PTHR23202:SF30. 1 hit.
PfamiPF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00246. WH2. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K1I7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPVPPPPAPP PPPTFALANT EKPTLNKTEQ AGRNALLSDI SKGKKLKKTV
60 70 80 90 100
TNDRSAPILD KPKGAGASAG GYGGGGGGGG GGGGGGGGSG GNFGGGGPPG
110 120 130 140 150
LGGLFQAGMP KLRSTANRDN DSGGSRPPIL PPGGRATSAK PFSPPSGPGR
160 170 180 190 200
FPAPSPGHRS GPPEPPRNRM PPPRPDVGSK PDSLPPPVPN TPRPVPSSLH
210 220 230 240 250
NRGSPAGLGA PRPPFPGNRG AAFGAGSARQ NPSGSSSPFP RPPLPPTPSR
260 270 280 290 300
ALDDKPPPPP PPVGNRPSMH REAVPPPPSQ TSKPPVPSTP RPGLGSQAPP
310 320 330 340 350
PPPPPSRPGP PPLPPASNDE IPRLPQRNLS LTSSAPPLPS PGRSGPLPPP
360 370 380 390 400
PSERPPPPVR DPPGRSGPLP PPPPINRNGS TARALPATPQ LPSRSGMDSP
410 420 430 440 450
RSGPRPPLPP DRPGAGAPPP PPPSTSVRNG FQDSSCEDEW ESRFYFHPIS
460 470 480 490
DLPPPEPYVP TTKTYPSKLA RNESRSGSNR RERGAPPLPP IPR
Length:493
Mass (Da):50,080
Last modified:October 1, 2002 - v1
Checksum:i0BADBE69463B5960
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ437262 mRNA. Translation: CAD24773.1.
AK031046 mRNA. Translation: BAC27228.1.
AK156547 mRNA. Translation: BAE33753.1.
BC049788 mRNA. Translation: AAH49788.1.
CCDSiCCDS16131.1.
RefSeqiNP_001276651.1. NM_001289722.1.
NP_001276652.1. NM_001289723.1.
NP_694778.1. NM_153138.4.
XP_006499187.1. XM_006499124.1.
XP_006499190.1. XM_006499127.1.
XP_006499191.1. XM_006499128.1.
UniGeneiMm.223504.

Genome annotation databases

EnsembliENSMUST00000094681; ENSMUSP00000092268; ENSMUSG00000075284.
ENSMUST00000102679; ENSMUSP00000099740; ENSMUSG00000075284.
ENSMUST00000102680; ENSMUSP00000099741; ENSMUSG00000075284.
GeneIDi215280.
KEGGimmu:215280.
UCSCiuc008kcu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ437262 mRNA. Translation: CAD24773.1 .
AK031046 mRNA. Translation: BAC27228.1 .
AK156547 mRNA. Translation: BAE33753.1 .
BC049788 mRNA. Translation: AAH49788.1 .
CCDSi CCDS16131.1.
RefSeqi NP_001276651.1. NM_001289722.1.
NP_001276652.1. NM_001289723.1.
NP_694778.1. NM_153138.4.
XP_006499187.1. XM_006499124.1.
XP_006499190.1. XM_006499127.1.
XP_006499191.1. XM_006499128.1.
UniGenei Mm.223504.

3D structure databases

ProteinModelPortali Q8K1I7.
SMRi Q8K1I7. Positions 29-60, 451-478.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 229611. 1 interaction.
IntActi Q8K1I7. 6 interactions.
MINTi MINT-260373.

PTM databases

PhosphoSitei Q8K1I7.

Proteomic databases

MaxQBi Q8K1I7.
PaxDbi Q8K1I7.
PRIDEi Q8K1I7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000094681 ; ENSMUSP00000092268 ; ENSMUSG00000075284 .
ENSMUST00000102679 ; ENSMUSP00000099740 ; ENSMUSG00000075284 .
ENSMUST00000102680 ; ENSMUSP00000099741 ; ENSMUSG00000075284 .
GeneIDi 215280.
KEGGi mmu:215280.
UCSCi uc008kcu.1. mouse.

Organism-specific databases

CTDi 7456.
MGIi MGI:2178801. Wipf1.

Phylogenomic databases

eggNOGi NOG149184.
GeneTreei ENSGT00760000119406.
HOGENOMi HOG000231914.
HOVERGENi HBG054157.
InParanoidi Q8K1I7.
OMAi QSSLHNR.
OrthoDBi EOG7PS1GQ.
PhylomeDBi Q8K1I7.
TreeFami TF332135.

Enzyme and pathway databases

Reactomei REACT_198374. Regulation of actin dynamics for phagocytic cup formation.

Miscellaneous databases

NextBioi 374662.
PROi Q8K1I7.
SOURCEi Search...

Gene expression databases

Bgeei Q8K1I7.
CleanExi MM_WIPF1.
ExpressionAtlasi Q8K1I7. baseline and differential.
Genevestigatori Q8K1I7.

Family and domain databases

InterProi IPR003124. WH2_dom.
IPR028295. WIP.
[Graphical view ]
PANTHERi PTHR23202:SF30. PTHR23202:SF30. 1 hit.
Pfami PF02205. WH2. 1 hit.
[Graphical view ]
SMARTi SM00246. WH2. 1 hit.
[Graphical view ]
PROSITEi PS51082. WH2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Phosphatidylinositol 4,5-biphosphate (PIP2)-induced vesicle movement depends on N-WASP and involves Nck, WIP, and Grb2."
    Benesch S., Lommel S., Steffen A., Stradal T.E.B., Scaplehorn N., Way M., Wehland J., Rottner K.
    J. Biol. Chem. 277:37771-37776(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH WASL.
    Strain: C57BL/6.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Spleen and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Limb.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143 AND SER-330, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "Actin-binding protein-1 interacts with WASp-interacting protein to regulate growth factor-induced dorsal ruffle formation."
    Cortesio C.L., Perrin B.J., Bennin D.A., Huttenlocher A.
    Mol. Biol. Cell 21:186-197(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DBNL.

Entry informationi

Entry nameiWIPF1_MOUSE
AccessioniPrimary (citable) accession number: Q8K1I7
Secondary accession number(s): Q3U0U8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Recruited to PIP5K-induced vesicle surfaces in the absence of functional WASL.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3