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Q8K1I7 (WIPF1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
WAS/WASL-interacting protein family member 1
Alternative name(s):
Wiskott-Aldrich syndrome protein-interacting protein
Short name=WASP-interacting protein
Gene names
Name:Wipf1
Synonyms:Waspip, Wip
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation By similarity. Plays a role in the formation of cell ruffles. Ref.1 Ref.5

Subunit structure

Binds to WAS within the N-terminal region, at a site distinct from the CDC42-binding site. Binds profilin and actin By similarity. Binds to WASL. Interacts with DBNL. Ref.1 Ref.5

Subcellular location

Cytoplasmic vesicle By similarity. Cytoplasmcytoskeleton By similarity. Cell projectionruffle. Note: Vesicle surfaces and along actin tails. Colocalizes with actin stress fibers. When coexpressed with WASL, no longer associated with actin filaments but accumulated in perinuclear and cortical areas like WASL By similarity. Ref.1 Ref.5

Miscellaneous

Recruited to PIP5K-induced vesicle surfaces in the absence of functional WASL.

Sequence similarities

Belongs to the verprolin family.

Contains 1 WH2 domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

WasP703153EBI-644216,EBI-644195

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 493493WAS/WASL-interacting protein family member 1
PRO_0000065942

Regions

Domain32 – 4918WH2
Repeat342 – 35110XRSGPXPPXP motif 1
Repeat364 – 37310XRSGPXPPXP motif 2
Repeat400 – 40910XRSGPXPPXP motif 3
Region45 – 484Binds actin By similarity
Compositional bias2 – 1312Poly-Pro
Compositional bias64 – 10845Gly-rich
Compositional bias127 – 492366Pro-rich

Amino acid modifications

Modified residue1431Phosphoserine Ref.4
Modified residue3301Phosphoserine Ref.4
Modified residue3401Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K1I7 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 0BADBE69463B5960

FASTA49350,080
        10         20         30         40         50         60 
MPVPPPPAPP PPPTFALANT EKPTLNKTEQ AGRNALLSDI SKGKKLKKTV TNDRSAPILD 

        70         80         90        100        110        120 
KPKGAGASAG GYGGGGGGGG GGGGGGGGSG GNFGGGGPPG LGGLFQAGMP KLRSTANRDN 

       130        140        150        160        170        180 
DSGGSRPPIL PPGGRATSAK PFSPPSGPGR FPAPSPGHRS GPPEPPRNRM PPPRPDVGSK 

       190        200        210        220        230        240 
PDSLPPPVPN TPRPVPSSLH NRGSPAGLGA PRPPFPGNRG AAFGAGSARQ NPSGSSSPFP 

       250        260        270        280        290        300 
RPPLPPTPSR ALDDKPPPPP PPVGNRPSMH REAVPPPPSQ TSKPPVPSTP RPGLGSQAPP 

       310        320        330        340        350        360 
PPPPPSRPGP PPLPPASNDE IPRLPQRNLS LTSSAPPLPS PGRSGPLPPP PSERPPPPVR 

       370        380        390        400        410        420 
DPPGRSGPLP PPPPINRNGS TARALPATPQ LPSRSGMDSP RSGPRPPLPP DRPGAGAPPP 

       430        440        450        460        470        480 
PPPSTSVRNG FQDSSCEDEW ESRFYFHPIS DLPPPEPYVP TTKTYPSKLA RNESRSGSNR 

       490 
RERGAPPLPP IPR 

« Hide

References

« Hide 'large scale' references
[1]"Phosphatidylinositol 4,5-biphosphate (PIP2)-induced vesicle movement depends on N-WASP and involves Nck, WIP, and Grb2."
Benesch S., Lommel S., Steffen A., Stradal T.E.B., Scaplehorn N., Way M., Wehland J., Rottner K.
J. Biol. Chem. 277:37771-37776(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH WASL.
Strain: C57BL/6.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Spleen and Thymus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Limb.
[4]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143 AND SER-330, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]"Actin-binding protein-1 interacts with WASp-interacting protein to regulate growth factor-induced dorsal ruffle formation."
Cortesio C.L., Perrin B.J., Bennin D.A., Huttenlocher A.
Mol. Biol. Cell 21:186-197(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DBNL.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ437262 mRNA. Translation: CAD24773.1.
AK031046 mRNA. Translation: BAC27228.1.
AK156547 mRNA. Translation: BAE33753.1.
BC049788 mRNA. Translation: AAH49788.1.
CCDSCCDS16131.1.
RefSeqNP_001276651.1. NM_001289722.1.
NP_001276652.1. NM_001289723.1.
NP_694778.1. NM_153138.4.
XP_006499187.1. XM_006499124.1.
XP_006499190.1. XM_006499127.1.
XP_006499191.1. XM_006499128.1.
UniGeneMm.223504.

3D structure databases

ProteinModelPortalQ8K1I7.
SMRQ8K1I7. Positions 29-60, 451-478.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid229611. 1 interaction.
IntActQ8K1I7. 6 interactions.
MINTMINT-260373.

PTM databases

PhosphoSiteQ8K1I7.

Proteomic databases

MaxQBQ8K1I7.
PaxDbQ8K1I7.
PRIDEQ8K1I7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000094681; ENSMUSP00000092268; ENSMUSG00000075284.
ENSMUST00000102679; ENSMUSP00000099740; ENSMUSG00000075284.
ENSMUST00000102680; ENSMUSP00000099741; ENSMUSG00000075284.
GeneID215280.
KEGGmmu:215280.
UCSCuc008kcu.1. mouse.

Organism-specific databases

CTD7456.
MGIMGI:2178801. Wipf1.

Phylogenomic databases

eggNOGNOG149184.
GeneTreeENSGT00750000117714.
HOGENOMHOG000231914.
HOVERGENHBG054157.
InParanoidQ8K1I7.
OMAQSSLHNR.
OrthoDBEOG7PS1GQ.
PhylomeDBQ8K1I7.
TreeFamTF332135.

Gene expression databases

ArrayExpressQ8K1I7.
BgeeQ8K1I7.
CleanExMM_WIPF1.
GenevestigatorQ8K1I7.

Family and domain databases

InterProIPR003124. WH2_dom.
IPR028295. WIP.
[Graphical view]
PANTHERPTHR23202:SF30. PTHR23202:SF30. 1 hit.
PfamPF02205. WH2. 1 hit.
[Graphical view]
SMARTSM00246. WH2. 1 hit.
[Graphical view]
PROSITEPS51082. WH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio374662.
PROQ8K1I7.
SOURCESearch...

Entry information

Entry nameWIPF1_MOUSE
AccessionPrimary (citable) accession number: Q8K1I7
Secondary accession number(s): Q3U0U8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot