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Q8K1I7

- WIPF1_MOUSE

UniProt

Q8K1I7 - WIPF1_MOUSE

Protein

WAS/WASL-interacting protein family member 1

Gene

Wipf1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Plays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation By similarity. Plays a role in the formation of cell ruffles.By similarity2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. actin filament-based movement Source: MGI
    2. actin polymerization or depolymerization Source: InterPro
    3. response to other organism Source: MGI

    Keywords - Ligandi

    Actin-binding

    Enzyme and pathway databases

    ReactomeiREACT_198374. Regulation of actin dynamics for phagocytic cup formation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    WAS/WASL-interacting protein family member 1
    Alternative name(s):
    Wiskott-Aldrich syndrome protein-interacting protein
    Short name:
    WASP-interacting protein
    Gene namesi
    Name:Wipf1
    Synonyms:Waspip, Wip
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:2178801. Wipf1.

    Subcellular locationi

    Cytoplasmic vesicle By similarity. Cytoplasmcytoskeleton By similarity. Cell projectionruffle 2 Publications
    Note: Vesicle surfaces and along actin tails. Colocalizes with actin stress fibers. When coexpressed with WASL, no longer associated with actin filaments but accumulated in perinuclear and cortical areas like WASL By similarity.By similarity

    GO - Cellular componenti

    1. actin cytoskeleton Source: MGI
    2. actin filament Source: MGI
    3. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
    4. ruffle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 493493WAS/WASL-interacting protein family member 1PRO_0000065942Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei143 – 1431Phosphoserine1 Publication
    Modified residuei330 – 3301Phosphoserine1 Publication
    Modified residuei340 – 3401PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8K1I7.
    PaxDbiQ8K1I7.
    PRIDEiQ8K1I7.

    PTM databases

    PhosphoSiteiQ8K1I7.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8K1I7.
    BgeeiQ8K1I7.
    CleanExiMM_WIPF1.
    GenevestigatoriQ8K1I7.

    Interactioni

    Subunit structurei

    Binds to WAS within the N-terminal region, at a site distinct from the CDC42-binding site. Binds profilin and actin By similarity. Binds to WASL. Interacts with DBNL.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    WasP703153EBI-644216,EBI-644195

    Protein-protein interaction databases

    BioGridi229611. 1 interaction.
    IntActiQ8K1I7. 6 interactions.
    MINTiMINT-260373.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K1I7.
    SMRiQ8K1I7. Positions 29-60, 451-478.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini32 – 4918WH2PROSITE-ProRule annotationAdd
    BLAST
    Repeati342 – 35110XRSGPXPPXP motif 1
    Repeati364 – 37310XRSGPXPPXP motif 2
    Repeati400 – 40910XRSGPXPPXP motif 3

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni45 – 484Binds actinBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi2 – 1312Poly-ProAdd
    BLAST
    Compositional biasi64 – 10845Gly-richAdd
    BLAST
    Compositional biasi127 – 492366Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the verprolin family.Curated
    Contains 1 WH2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG149184.
    GeneTreeiENSGT00750000117714.
    HOGENOMiHOG000231914.
    HOVERGENiHBG054157.
    InParanoidiQ8K1I7.
    OMAiQSSLHNR.
    OrthoDBiEOG7PS1GQ.
    PhylomeDBiQ8K1I7.
    TreeFamiTF332135.

    Family and domain databases

    InterProiIPR003124. WH2_dom.
    IPR028295. WIP.
    [Graphical view]
    PANTHERiPTHR23202:SF30. PTHR23202:SF30. 1 hit.
    PfamiPF02205. WH2. 1 hit.
    [Graphical view]
    SMARTiSM00246. WH2. 1 hit.
    [Graphical view]
    PROSITEiPS51082. WH2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8K1I7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPVPPPPAPP PPPTFALANT EKPTLNKTEQ AGRNALLSDI SKGKKLKKTV    50
    TNDRSAPILD KPKGAGASAG GYGGGGGGGG GGGGGGGGSG GNFGGGGPPG 100
    LGGLFQAGMP KLRSTANRDN DSGGSRPPIL PPGGRATSAK PFSPPSGPGR 150
    FPAPSPGHRS GPPEPPRNRM PPPRPDVGSK PDSLPPPVPN TPRPVPSSLH 200
    NRGSPAGLGA PRPPFPGNRG AAFGAGSARQ NPSGSSSPFP RPPLPPTPSR 250
    ALDDKPPPPP PPVGNRPSMH REAVPPPPSQ TSKPPVPSTP RPGLGSQAPP 300
    PPPPPSRPGP PPLPPASNDE IPRLPQRNLS LTSSAPPLPS PGRSGPLPPP 350
    PSERPPPPVR DPPGRSGPLP PPPPINRNGS TARALPATPQ LPSRSGMDSP 400
    RSGPRPPLPP DRPGAGAPPP PPPSTSVRNG FQDSSCEDEW ESRFYFHPIS 450
    DLPPPEPYVP TTKTYPSKLA RNESRSGSNR RERGAPPLPP IPR 493
    Length:493
    Mass (Da):50,080
    Last modified:October 1, 2002 - v1
    Checksum:i0BADBE69463B5960
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ437262 mRNA. Translation: CAD24773.1.
    AK031046 mRNA. Translation: BAC27228.1.
    AK156547 mRNA. Translation: BAE33753.1.
    BC049788 mRNA. Translation: AAH49788.1.
    CCDSiCCDS16131.1.
    RefSeqiNP_001276651.1. NM_001289722.1.
    NP_001276652.1. NM_001289723.1.
    NP_694778.1. NM_153138.4.
    XP_006499187.1. XM_006499124.1.
    XP_006499190.1. XM_006499127.1.
    XP_006499191.1. XM_006499128.1.
    UniGeneiMm.223504.

    Genome annotation databases

    EnsembliENSMUST00000094681; ENSMUSP00000092268; ENSMUSG00000075284.
    ENSMUST00000102679; ENSMUSP00000099740; ENSMUSG00000075284.
    ENSMUST00000102680; ENSMUSP00000099741; ENSMUSG00000075284.
    GeneIDi215280.
    KEGGimmu:215280.
    UCSCiuc008kcu.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ437262 mRNA. Translation: CAD24773.1 .
    AK031046 mRNA. Translation: BAC27228.1 .
    AK156547 mRNA. Translation: BAE33753.1 .
    BC049788 mRNA. Translation: AAH49788.1 .
    CCDSi CCDS16131.1.
    RefSeqi NP_001276651.1. NM_001289722.1.
    NP_001276652.1. NM_001289723.1.
    NP_694778.1. NM_153138.4.
    XP_006499187.1. XM_006499124.1.
    XP_006499190.1. XM_006499127.1.
    XP_006499191.1. XM_006499128.1.
    UniGenei Mm.223504.

    3D structure databases

    ProteinModelPortali Q8K1I7.
    SMRi Q8K1I7. Positions 29-60, 451-478.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 229611. 1 interaction.
    IntActi Q8K1I7. 6 interactions.
    MINTi MINT-260373.

    PTM databases

    PhosphoSitei Q8K1I7.

    Proteomic databases

    MaxQBi Q8K1I7.
    PaxDbi Q8K1I7.
    PRIDEi Q8K1I7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000094681 ; ENSMUSP00000092268 ; ENSMUSG00000075284 .
    ENSMUST00000102679 ; ENSMUSP00000099740 ; ENSMUSG00000075284 .
    ENSMUST00000102680 ; ENSMUSP00000099741 ; ENSMUSG00000075284 .
    GeneIDi 215280.
    KEGGi mmu:215280.
    UCSCi uc008kcu.1. mouse.

    Organism-specific databases

    CTDi 7456.
    MGIi MGI:2178801. Wipf1.

    Phylogenomic databases

    eggNOGi NOG149184.
    GeneTreei ENSGT00750000117714.
    HOGENOMi HOG000231914.
    HOVERGENi HBG054157.
    InParanoidi Q8K1I7.
    OMAi QSSLHNR.
    OrthoDBi EOG7PS1GQ.
    PhylomeDBi Q8K1I7.
    TreeFami TF332135.

    Enzyme and pathway databases

    Reactomei REACT_198374. Regulation of actin dynamics for phagocytic cup formation.

    Miscellaneous databases

    NextBioi 374662.
    PROi Q8K1I7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8K1I7.
    Bgeei Q8K1I7.
    CleanExi MM_WIPF1.
    Genevestigatori Q8K1I7.

    Family and domain databases

    InterProi IPR003124. WH2_dom.
    IPR028295. WIP.
    [Graphical view ]
    PANTHERi PTHR23202:SF30. PTHR23202:SF30. 1 hit.
    Pfami PF02205. WH2. 1 hit.
    [Graphical view ]
    SMARTi SM00246. WH2. 1 hit.
    [Graphical view ]
    PROSITEi PS51082. WH2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Phosphatidylinositol 4,5-biphosphate (PIP2)-induced vesicle movement depends on N-WASP and involves Nck, WIP, and Grb2."
      Benesch S., Lommel S., Steffen A., Stradal T.E.B., Scaplehorn N., Way M., Wehland J., Rottner K.
      J. Biol. Chem. 277:37771-37776(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH WASL.
      Strain: C57BL/6.
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Spleen and Thymus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Limb.
    4. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143 AND SER-330, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    5. "Actin-binding protein-1 interacts with WASp-interacting protein to regulate growth factor-induced dorsal ruffle formation."
      Cortesio C.L., Perrin B.J., Bennin D.A., Huttenlocher A.
      Mol. Biol. Cell 21:186-197(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DBNL.

    Entry informationi

    Entry nameiWIPF1_MOUSE
    AccessioniPrimary (citable) accession number: Q8K1I7
    Secondary accession number(s): Q3U0U8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Recruited to PIP5K-induced vesicle surfaces in the absence of functional WASL.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3