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Q8K1H1

- TDRD7_MOUSE

UniProt

Q8K1H1 - TDRD7_MOUSE

Protein

Tudor domain-containing protein 7

Gene

Tdrd7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Component of specific cytoplasmic RNA granules involved in post-transcriptional regulation of specific genes: probably acts by binding to specific mRNAs and regulating their translation. Required for lens transparency during lens development, by regulating translation of genes such as CRYBB3 and HSPB1 in the developing lens. Also required during spermatogenesis.1 Publication

    GO - Molecular functioni

    1. mRNA binding Source: UniProtKB
    2. protein binding Source: MGI

    GO - Biological processi

    1. germ cell development Source: MGI
    2. lens fiber cell differentiation Source: UniProtKB
    3. lens morphogenesis in camera-type eye Source: UniProtKB
    4. posttranscriptional regulation of gene expression Source: UniProtKB
    5. spermatogenesis Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Spermatogenesis

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tudor domain-containing protein 7
    Alternative name(s):
    PCTAIRE2-binding protein
    Tudor repeat associator with PCTAIRE-2
    Short name:
    Trap
    Gene namesi
    Name:Tdrd7
    Synonyms:Pctaire2bp
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:2140279. Tdrd7.

    Subcellular locationi

    Cytoplasm 3 Publications
    Note: Localizes to cytoplasmic RNA granules. Present in chromatoid body (CB) of spermatids (mammalian counterpart of germplasm, pole plasm or polar granules in Drosophila germ cells), also named processing bodies (P-bodies) in somatic cells. Detected in the multilobular cytoplasmic CBs (also called intermitochondrial cementin) in pachytene spermatocytes and as a single perinuclear CB in haploid round spermatids.

    GO - Cellular componenti

    1. chromatoid body Source: UniProtKB
    2. cytoplasm Source: UniProtKB
    3. mitochondrial matrix Source: Ensembl
    4. P granule Source: MGI
    5. ribonucleoprotein complex Source: MGI
    6. ribonucleoprotein granule Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Mice develop cataracts and glaucoma and males are sterile. Within 4 weeks of birth, mice develop a posterior cataract that becomes severe with age. At later stages, the lens fiber cell compartment develops vacuoles with lens capsule rupture and extrusion of fiber cell mass into the vitreous. In addition, the mass of fiber cells passes through the pupil into the anterior chamber of the eye. By 4 months of age, iris flattening is detected and anterior chamber depth increased. By 6 months of age, the intraocular pressure (IOP) is elevated in some mutants, and the incidence of elevated IOP increases with age, leading to glaucome. Severe optic nerve atrophy characterized by retinal ganglion cell axon loss and excavative remodeling of the optic nerve are observed. In addition, males display sterility due to an arrest in spermatogenesis at the round spermatid stage, likely due to a chromatoid body defect.1 Publication

    Keywords - Diseasei

    Cataract

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10861086Tudor domain-containing protein 7PRO_0000183170Add
    BLAST

    Proteomic databases

    MaxQBiQ8K1H1.
    PaxDbiQ8K1H1.
    PRIDEiQ8K1H1.

    PTM databases

    PhosphoSiteiQ8K1H1.

    Expressioni

    Tissue specificityi

    Mainly expressed in testis. Expressed in spermatogonia, spermatocytes and round spermatids (at protein level). Also expressed in the developing lens.2 Publications

    Developmental stagei

    At embryonic day E12.5, it is expressed in differentiating fiber cells in the posterior lens, but not in the anterior epithelium of the lens (AEL).1 Publication

    Gene expression databases

    ArrayExpressiQ8K1H1.
    BgeeiQ8K1H1.
    CleanExiMM_TDRD7.
    GenevestigatoriQ8K1H1.

    Interactioni

    Subunit structurei

    Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. Found in a complex containing CABLES1, CDK16 and CDK17. Interacts with CABLES1, CDK17 and PIWIL1.3 Publications

    Structurei

    Secondary structure

    1
    1086
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 1715
    Helixi24 – 3512
    Helixi41 – 444
    Helixi49 – 546
    Turni57 – 593
    Beta strandi60 – 645
    Beta strandi70 – 745
    Helixi223 – 23715
    Turni243 – 2453
    Helixi246 – 2549
    Helixi260 – 2689
    Turni270 – 2723
    Beta strandi273 – 2764
    Turni279 – 2813
    Beta strandi286 – 2894
    Helixi290 – 2923
    Beta strandi294 – 2963
    Helixi301 – 3044
    Beta strandi309 – 3124
    Helixi313 – 3153
    Helixi328 – 33811
    Helixi348 – 35710
    Helixi363 – 3664
    Helixi369 – 3757
    Beta strandi376 – 3838
    Turni384 – 3863
    Beta strandi387 – 3926

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LH9NMR-A1-76[»]
    2LY1NMR-A223-400[»]
    2LY2NMR-A223-400[»]
    ProteinModelPortaliQ8K1H1.
    SMRiQ8K1H1. Positions 1-76, 223-400, 651-775, 958-1029.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 7674HTH OST-type 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini222 – 29170HTH OST-type 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini325 – 39470HTH OST-type 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini501 – 55858Tudor 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini691 – 74858Tudor 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni849 – 1086238Interacts with CDK17By similarityAdd
    BLAST
    Regioni881 – 1086206Interacts with CABLES1Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi307 – 3137Poly-Pro

    Sequence similaritiesi

    Belongs to the TDRD7 family.Curated
    Contains 3 HTH OST-type domains.PROSITE-ProRule annotation
    Contains 2 Tudor domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG256635.
    GeneTreeiENSGT00750000117678.
    HOGENOMiHOG000294063.
    HOVERGENiHBG055704.
    InParanoidiB1AWH5.
    OrthoDBiEOG7BW0HQ.

    Family and domain databases

    InterProiIPR025605. OST-HTH/LOTUS_dom.
    IPR002999. Tudor.
    [Graphical view]
    PfamiPF12872. OST-HTH. 1 hit.
    PF00567. TUDOR. 3 hits.
    [Graphical view]
    SMARTiSM00333. TUDOR. 3 hits.
    [Graphical view]
    PROSITEiPS51644. HTH_OST. 3 hits.
    PS50304. TUDOR. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8K1H1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLEADLVSKM LRAVLQSHKN GIVLPRLQGE YRSLTGDWIP FKQLGYPTLE     50
    AYLRSVPAVV RIEASRSGEI VCYAVACTET ARIAQLVARQ RTSKRKIGRQ 100
    INCQMRVKKA MPFFLEGKPK ATLRQPGFAS DYSISRKPNS ALLRDRGSAL 150
    GVKADVDMPP YPDTPVQRHA SMSANSRFSP KSSLPASFQT HISRACPTEV 200
    NDNLNQTVEK PNITPPASYT NKMDEVQNRI KEILDKHNNG IWISKLPHFY 250
    KEFYKEDLNQ GVLQQFEHWP HICTVEKPCG GGQDSLLYPA RREQPLKSDQ 300
    DPEKELPPPP PAPKQEVPSQ GSPAVMPDVK EKVAELLGKY SSGLWASALP 350
    KAFEDMYKVK FPEDALKNLA SLSDVCTINY ISGNTQKAIL YAKLPLPTDK 400
    ILKDEGQAQG DFDIKSMIEQ EYLQIEKNMA ESADEFLEDI TVPPLVIPTE 450
    ASPSVLVVEL SNTNDVVIRY VGKDYSAAQE LMEDEMKEFY SKNPRVTPIQ 500
    TVHVGQLLAV NAEEDAWLRA QIISTDENKI KVCYVDYGFC ENIEKSKAYR 550
    LNPRFCSLSF QATKCKLAGL EVLNDDPDLV KAVESLTCGK IFAVEILDKS 600
    DVPLVVLYDT SGEDDININA TCLKAICDRS LQVHLQVDAM YTNVKVTNIC 650
    SDGTLYCQVP CKGLNKLNDL LHKTEDYFHC KHMTSEYFIS LPFCGKICLF 700
    HCKGKWLRVE ITNVHSSRAL DVQFLDSGNS TSVKVSELRE IPPRFLQEML 750
    AIPPQAIKCC LADLPQSIGM WTPDAVLWLR DSVLNCSDCS IKVTKMDETK 800
    GVAYVYLFTP NNFPDPHRSI NRQITNADLW KHQKDVFLSA VSTAASSPGN 850
    RNGGTPAPGS PAESLRKSHP EVIKKSVLDH TSSFSLEELP PPVHLSRSGE 900
    HMDVYVPVAC HPGHFVIQPW QEIHKLEVLM EEMILYYSVS EERHIAVERD 950
    QVYAAKVENK WYRVLLKGIL TNGLVSVYEL DYGKHELVNI RKVQPLVDVF 1000
    RKLPFQAVTA QLAGVKCSQW SEEASMVFRN HVEKKALVAL VQTVVEHTNP 1050
    WDRKVVLYLV DTSLPDTDTW IHDFMSQYLL ELSKVN 1086
    Length:1,086
    Mass (Da):122,174
    Last modified:October 1, 2002 - v1
    Checksum:i90FF74E1CDD24F86
    GO

    Sequence cautioni

    The sequence CAM17034.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAM22130.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL732615 Genomic DNA. Translation: CAM17026.1.
    AL732615, AL772381 Genomic DNA. Translation: CAM17034.1. Sequence problems.
    AL772381, AL732615 Genomic DNA. Translation: CAM22130.1. Sequence problems.
    BC025099 mRNA. Translation: AAH25099.1.
    BC029689 mRNA. Translation: AAH29689.1.
    CCDSiCCDS18142.1.
    RefSeqiNP_001277404.1. NM_001290475.1.
    NP_666254.1. NM_146142.2.
    XP_006537580.1. XM_006537517.1.
    UniGeneiMm.275413.

    Genome annotation databases

    EnsembliENSMUST00000102929; ENSMUSP00000099993; ENSMUSG00000035517.
    GeneIDi100121.
    KEGGimmu:100121.
    UCSCiuc008stb.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL732615 Genomic DNA. Translation: CAM17026.1 .
    AL732615 , AL772381 Genomic DNA. Translation: CAM17034.1 . Sequence problems.
    AL772381 , AL732615 Genomic DNA. Translation: CAM22130.1 . Sequence problems.
    BC025099 mRNA. Translation: AAH25099.1 .
    BC029689 mRNA. Translation: AAH29689.1 .
    CCDSi CCDS18142.1.
    RefSeqi NP_001277404.1. NM_001290475.1.
    NP_666254.1. NM_146142.2.
    XP_006537580.1. XM_006537517.1.
    UniGenei Mm.275413.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LH9 NMR - A 1-76 [» ]
    2LY1 NMR - A 223-400 [» ]
    2LY2 NMR - A 223-400 [» ]
    ProteinModelPortali Q8K1H1.
    SMRi Q8K1H1. Positions 1-76, 223-400, 651-775, 958-1029.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8K1H1.

    Proteomic databases

    MaxQBi Q8K1H1.
    PaxDbi Q8K1H1.
    PRIDEi Q8K1H1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000102929 ; ENSMUSP00000099993 ; ENSMUSG00000035517 .
    GeneIDi 100121.
    KEGGi mmu:100121.
    UCSCi uc008stb.1. mouse.

    Organism-specific databases

    CTDi 23424.
    MGIi MGI:2140279. Tdrd7.

    Phylogenomic databases

    eggNOGi NOG256635.
    GeneTreei ENSGT00750000117678.
    HOGENOMi HOG000294063.
    HOVERGENi HBG055704.
    InParanoidi B1AWH5.
    OrthoDBi EOG7BW0HQ.

    Miscellaneous databases

    ChiTaRSi TDRD7. mouse.
    NextBioi 354271.
    PROi Q8K1H1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8K1H1.
    Bgeei Q8K1H1.
    CleanExi MM_TDRD7.
    Genevestigatori Q8K1H1.

    Family and domain databases

    InterProi IPR025605. OST-HTH/LOTUS_dom.
    IPR002999. Tudor.
    [Graphical view ]
    Pfami PF12872. OST-HTH. 1 hit.
    PF00567. TUDOR. 3 hits.
    [Graphical view ]
    SMARTi SM00333. TUDOR. 3 hits.
    [Graphical view ]
    PROSITEi PS51644. HTH_OST. 3 hits.
    PS50304. TUDOR. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Eye and Retina.
    3. Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH CABLES1 AND CDK17, INTERACTION WITH CABLES1.
      Tissue: Brain.
    4. "Tudor-related proteins TDRD1/MTR-1, TDRD6 and TDRD7/TRAP: domain composition, intracellular localization, and function in male germ cells in mice."
      Hosokawa M., Shoji M., Kitamura K., Tanaka T., Noce T., Chuma S., Nakatsuji N.
      Dev. Biol. 301:38-52(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A MRNP COMPLEX, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    5. "Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members."
      Vagin V.V., Wohlschlegel J., Qu J., Jonsson Z., Huang X., Chuma S., Girard A., Sachidanandam R., Hannon G.J., Aravin A.A.
      Genes Dev. 23:1749-1762(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PIWIL1.
    6. Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiTDRD7_MOUSE
    AccessioniPrimary (citable) accession number: Q8K1H1
    Secondary accession number(s): B1AWG7, B1AWH5, Q8R181
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2004
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3