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Q8K1H1 (TDRD7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tudor domain-containing protein 7
Alternative name(s):
PCTAIRE2-binding protein
Tudor repeat associator with PCTAIRE-2
Short name=Trap
Gene names
Name:Tdrd7
Synonyms:Pctaire2bp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1086 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of specific cytoplasmic RNA granules involved in post-transcriptional regulation of specific genes: probably acts by binding to specific mRNAs and regulating their translation. Required for lens transparency during lens development, by regulating translation of genes such as CRYBB3 and HSPB1 in the developing lens. Also required during spermatogenesis. Ref.6

Subunit structure

Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. Found in a complex containing CABLES1, CDK16 and CDK17. Interacts with CABLES1, CDK17 and PIWIL1. Ref.3 Ref.4 Ref.5

Subcellular location

Cytoplasm. Note: Localizes to cytoplasmic RNA granules. Present in chromatoid body (CB) of spermatids (mammalian counterpart of germplasm, pole plasm or polar granules in Drosophila germ cells), also named processing bodies (P-bodies) in somatic cells. Detected in the multilobular cytoplasmic CBs (also called intermitochondrial cementin) in pachytene spermatocytes and as a single perinuclear CB in haploid round spermatids. Ref.3 Ref.4 Ref.6

Tissue specificity

Mainly expressed in testis. Expressed in spermatogonia, spermatocytes and round spermatids (at protein level). Also expressed in the developing lens. Ref.4 Ref.6

Developmental stage

At embryonic day E12.5, it is expressed in differentiating fiber cells in the posterior lens, but not in the anterior epithelium of the lens (AEL). Ref.6

Disruption phenotype

Mice develop cataracts and glaucoma and males are sterile. Within 4 weeks of birth, mice develop a posterior cataract that becomes severe with age. At later stages, the lens fiber cell compartment develops vacuoles with lens capsule rupture and extrusion of fiber cell mass into the vitreous. In addition, the mass of fiber cells passes through the pupil into the anterior chamber of the eye. By 4 months of age, iris flattening is detected and anterior chamber depth increased. By 6 months of age, the intraocular pressure (IOP) is elevated in some mutants, and the incidence of elevated IOP increases with age, leading to glaucome. Severe optic nerve atrophy characterized by retinal ganglion cell axon loss and excavative remodeling of the optic nerve are observed. In addition, males display sterility due to an arrest in spermatogenesis at the round spermatid stage, likely due to a chromatoid body defect. Ref.6

Sequence similarities

Belongs to the TDRD7 family.

Contains 3 HTH OST-type domains.

Contains 2 Tudor domains.

Sequence caution

The sequence CAM17034.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAM22130.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10861086Tudor domain-containing protein 7
PRO_0000183170

Regions

Domain3 – 7674HTH OST-type 1
Domain222 – 29170HTH OST-type 2
Domain325 – 39470HTH OST-type 3
Domain501 – 55858Tudor 1
Domain691 – 74858Tudor 2
Region849 – 1086238Interacts with CDK17 By similarity
Region881 – 1086206Interacts with CABLES1
Compositional bias307 – 3137Poly-Pro

Secondary structure

............................................... 1086
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8K1H1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 90FF74E1CDD24F86

FASTA1,086122,174
        10         20         30         40         50         60 
MLEADLVSKM LRAVLQSHKN GIVLPRLQGE YRSLTGDWIP FKQLGYPTLE AYLRSVPAVV 

        70         80         90        100        110        120 
RIEASRSGEI VCYAVACTET ARIAQLVARQ RTSKRKIGRQ INCQMRVKKA MPFFLEGKPK 

       130        140        150        160        170        180 
ATLRQPGFAS DYSISRKPNS ALLRDRGSAL GVKADVDMPP YPDTPVQRHA SMSANSRFSP 

       190        200        210        220        230        240 
KSSLPASFQT HISRACPTEV NDNLNQTVEK PNITPPASYT NKMDEVQNRI KEILDKHNNG 

       250        260        270        280        290        300 
IWISKLPHFY KEFYKEDLNQ GVLQQFEHWP HICTVEKPCG GGQDSLLYPA RREQPLKSDQ 

       310        320        330        340        350        360 
DPEKELPPPP PAPKQEVPSQ GSPAVMPDVK EKVAELLGKY SSGLWASALP KAFEDMYKVK 

       370        380        390        400        410        420 
FPEDALKNLA SLSDVCTINY ISGNTQKAIL YAKLPLPTDK ILKDEGQAQG DFDIKSMIEQ 

       430        440        450        460        470        480 
EYLQIEKNMA ESADEFLEDI TVPPLVIPTE ASPSVLVVEL SNTNDVVIRY VGKDYSAAQE 

       490        500        510        520        530        540 
LMEDEMKEFY SKNPRVTPIQ TVHVGQLLAV NAEEDAWLRA QIISTDENKI KVCYVDYGFC 

       550        560        570        580        590        600 
ENIEKSKAYR LNPRFCSLSF QATKCKLAGL EVLNDDPDLV KAVESLTCGK IFAVEILDKS 

       610        620        630        640        650        660 
DVPLVVLYDT SGEDDININA TCLKAICDRS LQVHLQVDAM YTNVKVTNIC SDGTLYCQVP 

       670        680        690        700        710        720 
CKGLNKLNDL LHKTEDYFHC KHMTSEYFIS LPFCGKICLF HCKGKWLRVE ITNVHSSRAL 

       730        740        750        760        770        780 
DVQFLDSGNS TSVKVSELRE IPPRFLQEML AIPPQAIKCC LADLPQSIGM WTPDAVLWLR 

       790        800        810        820        830        840 
DSVLNCSDCS IKVTKMDETK GVAYVYLFTP NNFPDPHRSI NRQITNADLW KHQKDVFLSA 

       850        860        870        880        890        900 
VSTAASSPGN RNGGTPAPGS PAESLRKSHP EVIKKSVLDH TSSFSLEELP PPVHLSRSGE 

       910        920        930        940        950        960 
HMDVYVPVAC HPGHFVIQPW QEIHKLEVLM EEMILYYSVS EERHIAVERD QVYAAKVENK 

       970        980        990       1000       1010       1020 
WYRVLLKGIL TNGLVSVYEL DYGKHELVNI RKVQPLVDVF RKLPFQAVTA QLAGVKCSQW 

      1030       1040       1050       1060       1070       1080 
SEEASMVFRN HVEKKALVAL VQTVVEHTNP WDRKVVLYLV DTSLPDTDTW IHDFMSQYLL 


ELSKVN 

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Eye and Retina.
[3]"ik3-1/Cables is associated with Trap and Pctaire2."
Yamochi T., Nishimoto I., Okuda T., Matsuoka M.
Biochem. Biophys. Res. Commun. 286:1045-1050(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH CABLES1 AND CDK17, INTERACTION WITH CABLES1.
Tissue: Brain.
[4]"Tudor-related proteins TDRD1/MTR-1, TDRD6 and TDRD7/TRAP: domain composition, intracellular localization, and function in male germ cells in mice."
Hosokawa M., Shoji M., Kitamura K., Tanaka T., Noce T., Chuma S., Nakatsuji N.
Dev. Biol. 301:38-52(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A MRNP COMPLEX, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]"Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members."
Vagin V.V., Wohlschlegel J., Qu J., Jonsson Z., Huang X., Chuma S., Girard A., Sachidanandam R., Hannon G.J., Aravin A.A.
Genes Dev. 23:1749-1762(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PIWIL1.
[6]"Mutations in the RNA granule component TDRD7 cause cataract and glaucoma."
Lachke S.A., Alkuraya F.S., Kneeland S.C., Ohn T., Aboukhalil A., Howell G.R., Saadi I., Cavallesco R., Yue Y., Tsai A.C., Nair K.S., Cosma M.I., Smith R.S., Hodges E., Alfadhli S.M., Al-Hajeri A., Shamseldin H.E., Behbehani A. expand/collapse author list , Hannon G.J., Bulyk M.L., Drack A.V., Anderson P.J., John S.W., Maas R.L.
Science 331:1571-1576(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL732615 Genomic DNA. Translation: CAM17026.1.
AL732615, AL772381 Genomic DNA. Translation: CAM17034.1. Sequence problems.
AL772381, AL732615 Genomic DNA. Translation: CAM22130.1. Sequence problems.
BC025099 mRNA. Translation: AAH25099.1.
BC029689 mRNA. Translation: AAH29689.1.
CCDSCCDS18142.1.
RefSeqNP_001277404.1. NM_001290475.1.
NP_666254.1. NM_146142.2.
XP_006537580.1. XM_006537517.1.
UniGeneMm.275413.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2LH9NMR-A1-76[»]
2LY1NMR-A223-400[»]
2LY2NMR-A223-400[»]
ProteinModelPortalQ8K1H1.
SMRQ8K1H1. Positions 1-76, 223-400, 651-775, 958-1029.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8K1H1.

Proteomic databases

MaxQBQ8K1H1.
PaxDbQ8K1H1.
PRIDEQ8K1H1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102929; ENSMUSP00000099993; ENSMUSG00000035517.
GeneID100121.
KEGGmmu:100121.
UCSCuc008stb.1. mouse.

Organism-specific databases

CTD23424.
MGIMGI:2140279. Tdrd7.

Phylogenomic databases

eggNOGNOG256635.
GeneTreeENSGT00750000117678.
HOGENOMHOG000294063.
HOVERGENHBG055704.
InParanoidB1AWH5.
OrthoDBEOG7BW0HQ.

Gene expression databases

ArrayExpressQ8K1H1.
BgeeQ8K1H1.
CleanExMM_TDRD7.
GenevestigatorQ8K1H1.

Family and domain databases

InterProIPR025605. OST-HTH/LOTUS_dom.
IPR002999. Tudor.
[Graphical view]
PfamPF12872. OST-HTH. 1 hit.
PF00567. TUDOR. 3 hits.
[Graphical view]
SMARTSM00333. TUDOR. 3 hits.
[Graphical view]
PROSITEPS51644. HTH_OST. 3 hits.
PS50304. TUDOR. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTDRD7. mouse.
NextBio354271.
PROQ8K1H1.
SOURCESearch...

Entry information

Entry nameTDRD7_MOUSE
AccessionPrimary (citable) accession number: Q8K1H1
Secondary accession number(s): B1AWG7, B1AWH5, Q8R181
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot