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Protein

Limbin

Gene

Evc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Positive regulator of the hedgehog signaling pathway. Plays a critical role in bone formation and skeletal development. May be involved in early embryonic morphogenesis.1 Publication

GO - Biological processi

  • smoothened signaling pathway Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-5632684. Hedgehog 'on' state.
R-MMU-5635838. Activation of SMO.

Names & Taxonomyi

Protein namesi
Recommended name:
Limbin
Gene namesi
Name:Evc2
Synonyms:Lbn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1915775. Evc2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 210ExtracellularSequence analysisAdd BLAST181
Transmembranei211 – 231HelicalSequence analysisAdd BLAST21
Topological domaini232 – 1220CytoplasmicSequence analysisAdd BLAST989

GO - Cellular componenti

  • ciliary membrane Source: Reactome
  • cilium Source: UniProtKB
  • cytoplasm Source: UniProtKB-KW
  • cytoskeleton Source: UniProtKB-KW
  • integral component of membrane Source: UniProtKB-KW
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoskeleton, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000008436430 – 1220LimbinAdd BLAST1191

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi100N-linked (GlcNAc...)Sequence analysis1
Glycosylationi109N-linked (GlcNAc...)Sequence analysis1
Glycosylationi130N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8K1G2.
PaxDbiQ8K1G2.
PRIDEiQ8K1G2.

PTM databases

iPTMnetiQ8K1G2.
PhosphoSitePlusiQ8K1G2.

Expressioni

Tissue specificityi

Expressed in long and cranial bones, kidney and heart. Strongly expressed in proliferating chondrocytes, osteoblasts and osteoclasts.1 Publication

Developmental stagei

Found in the embryo at day E7, E11, E15, and E17. At the limb bud formation stage E11, it is expressed in fore- and hindlimb buds, branchial arches, and facial primordia.

Gene expression databases

BgeeiENSMUSG00000050248.
CleanExiMM_EVC2.
GenevisibleiQ8K1G2. MM.

Interactioni

Subunit structurei

Interacts with EVC.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055130.

Structurei

3D structure databases

ProteinModelPortaliQ8K1G2.
SMRiQ8K1G2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili355 – 404Sequence analysisAdd BLAST50
Coiled coili563 – 644Sequence analysisAdd BLAST82
Coiled coili854 – 875Sequence analysisAdd BLAST22
Coiled coili920 – 1005Sequence analysisAdd BLAST86

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGSC. Eukaryota.
ENOG4111Y8B. LUCA.
GeneTreeiENSGT00390000014437.
HOGENOMiHOG000113288.
HOVERGENiHBG045574.
InParanoidiQ8K1G2.
KOiK19608.
OMAiTQILESH.
OrthoDBiEOG091G0FQY.
PhylomeDBiQ8K1G2.
TreeFamiTF331379.

Family and domain databases

InterProiIPR022076. Limbin.
IPR026501. Limbin/Ellis-van_Creveld.
[Graphical view]
PANTHERiPTHR16795. PTHR16795. 1 hit.
PfamiPF12297. EVC2_like. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K1G2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGATGPTGAG GRATWVLAGN ILAAALVLGS GPRALPPSFP ALGPGSPSRP
60 70 80 90 100
GPAGPWASSQ YSDISREARG PFENGVIFQK CSLVSGQSES QTMHVQLSVN
110 120 130 140 150
NTRTPTSVNL SNLLVLDEIT GLAVKESPGN NTQDGIQTFR KSFLQVGECY
160 170 180 190 200
SVSYTASLDP TALGTGESLD LPARLIFQSP SQNRTQLKAP FTITVEEKIM
210 220 230 240 250
VLPNHGLHAA GFIAAFLISL LLTVAALFFL ARGRCLQGGM LSRCRIQHPE
260 270 280 290 300
NKLEPSPFTS ANGVSQDLSL NDQVVAILTS EEPGSMLQAL EELEIATLNQ
310 320 330 340 350
ADADLEACRN QISKDIIALL MKNLVSGGHL SPQTERKMAA AFKKQFLLLE
360 370 380 390 400
NEIQEEYERK MLALTAECDL EMRKKTENQY QREMVAMEEA EEVLKRVSER
410 420 430 440 450
SAAECSSLLR TLHGLEQEDM QRSLTLDQAE DFAQAHRQLA VFQRNELHSI
460 470 480 490 500
VYTQIQSAVS KGELRPEVAK MMLQDYSKTQ ESVEELMDFF QATKRYHLSK
510 520 530 540 550
RFGHREYLVQ RLQAMETRVQ GLLNTAATQL TSLIHKHERA GYLDEDQMET
560 570 580 590 600
LLERAQTETF SIKQKLDNDL KQEKKRLHQR LITRRRRELL QKHKEQQKEQ
610 620 630 640 650
VSLGEASSTA EDAVQYLHQW RSVMAEHTAA LEELQERLDQ AALDDLRVLT
660 670 680 690 700
VSLSEKATEE LRRLQSTAMT QELLKRSAPW LFLQQILEEH SRESAARTTQ
710 720 730 740 750
LEAEERERGQ ELVQGVRQRL QQDALEAYTE EQAELRHWEH LVFMKLCCAA
760 770 780 790 800
ISLSEEDLLR VRQEAQGCFS QLDRSLALPR VRARVLQQQA QMAWREAEFR
810 820 830 840 850
KLDQALAAPE LQSKARKLRS KGRGKADLLK KNLEDKIRLF EERAPVELAD
860 870 880 890 900
QVRGELLQER VQRLEAQEAH FAESLVALQF QKVARAAETL SVYTALLSIQ
910 920 930 940 950
DLLLGELSES ETLTKSACVQ ILESHRPELQ ELQELERKLE DQLVQQEEAE
960 970 980 990 1000
QQRVLESWQR WAADGPGLSE PEEMDPERQV SAILRQALNK GQKLLEQHQQ
1010 1020 1030 1040 1050
RVREEWQNGA VLEDSLESIE ADTMASLCSQ GLRLVSYLSR MTMVPGSTLL
1060 1070 1080 1090 1100
RLLSVVLPAA SQPQLLALLD AVSEKHSDHT AENESSGEQA QAEQSKRRKH
1110 1120 1130 1140 1150
QVWWKVLDSR FRADLVSQGL ERMLWARQKK ERILKKIYVP VQERVMFPGK
1160 1170 1180 1190 1200
GSWPHLSLEP IGELAPIPIT GADAMDILNT GEKIFVFRSP REPEISLRVP
1210 1220
PRRKKNFLNA KKANRALGLD
Length:1,220
Mass (Da):137,639
Last modified:October 1, 2002 - v1
Checksum:iE67671714A866B1D
GO

Sequence cautioni

The sequence BAC32167 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083066 mRNA. Translation: BAC06589.1.
BC037473 mRNA. Translation: AAH37473.1.
BC064473 mRNA. Translation: AAH64473.1.
AK044977 mRNA. Translation: BAC32167.1. Different initiation.
CCDSiCCDS19248.1.
RefSeqiNP_666032.1. NM_145920.3.
UniGeneiMm.25506.

Genome annotation databases

EnsembliENSMUST00000056365; ENSMUSP00000055130; ENSMUSG00000050248.
GeneIDi68525.
KEGGimmu:68525.
UCSCiuc008xfr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083066 mRNA. Translation: BAC06589.1.
BC037473 mRNA. Translation: AAH37473.1.
BC064473 mRNA. Translation: AAH64473.1.
AK044977 mRNA. Translation: BAC32167.1. Different initiation.
CCDSiCCDS19248.1.
RefSeqiNP_666032.1. NM_145920.3.
UniGeneiMm.25506.

3D structure databases

ProteinModelPortaliQ8K1G2.
SMRiQ8K1G2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055130.

PTM databases

iPTMnetiQ8K1G2.
PhosphoSitePlusiQ8K1G2.

Proteomic databases

MaxQBiQ8K1G2.
PaxDbiQ8K1G2.
PRIDEiQ8K1G2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000056365; ENSMUSP00000055130; ENSMUSG00000050248.
GeneIDi68525.
KEGGimmu:68525.
UCSCiuc008xfr.2. mouse.

Organism-specific databases

CTDi132884.
MGIiMGI:1915775. Evc2.

Phylogenomic databases

eggNOGiENOG410IGSC. Eukaryota.
ENOG4111Y8B. LUCA.
GeneTreeiENSGT00390000014437.
HOGENOMiHOG000113288.
HOVERGENiHBG045574.
InParanoidiQ8K1G2.
KOiK19608.
OMAiTQILESH.
OrthoDBiEOG091G0FQY.
PhylomeDBiQ8K1G2.
TreeFamiTF331379.

Enzyme and pathway databases

ReactomeiR-MMU-5632684. Hedgehog 'on' state.
R-MMU-5635838. Activation of SMO.

Miscellaneous databases

PROiQ8K1G2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000050248.
CleanExiMM_EVC2.
GenevisibleiQ8K1G2. MM.

Family and domain databases

InterProiIPR022076. Limbin.
IPR026501. Limbin/Ellis-van_Creveld.
[Graphical view]
PANTHERiPTHR16795. PTHR16795. 1 hit.
PfamiPF12297. EVC2_like. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLBN_MOUSE
AccessioniPrimary (citable) accession number: Q8K1G2
Secondary accession number(s): Q8BRF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.